pPGK-Fluc-Ripk1(mouse)-3UTR-SV40-Rluc
$70.00
In stock
SKU
PL53820
100μg liquid plasmid(Instantly):$91
| name | pPGK-Fluc-Ripk1(mouse)-3UTR-SV40-Rluc | another name | apPGK-Fluc-Ripk1(mouse)-3UTR-SV40-Rluc |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Luciferase Report | Fragment type | UTR |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Rluc,Fluc | |
| Promoter | PGK | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG GTTTTTTTAA GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTTTA ACAAAATATT AACGCTTACA ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGCG GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGATTCT TCTGACACAA CAGTCTCGAA CCAAAGGCTG GAGCCACCAT GGCTTCCAAG GTGTACGACC CCGAGCAACG CAAACGCATG ATCACTGGGC CTCAGTGGTG GGCTCGCTGC AAGCAAATGA ACGTGCTGGA CTCCTTCATC AACTACTATG ATTCCGAGAA GCACGCCGAG AACGCCGTGA TTTTTCTGCA TGGTAACGCT GCCTCCAGCT ACCTGTGGAG GCACGTCGTG CCTCACATCG AGCCCGTGGC TAGATGCATC ATCCCTGATC TGATCGGAAT GGGTAAGTCC GGCAAGAGCG GGAATGGCTC ATATCGCCTC CTGGATCACT ACAAGTACCT CACCGCTTGG TTCGAGCTGC TGAACCTTCC AAAGAAAATC ATCTTTGTGG GCCACGACTG GGGGGCTTGT CTGGCCTTTC ACTACTCCTA CGAGCACCAA GACAAGATCA AGGCCATCGT CCATGCTGAG AGTGTCGTGG ACGTGATCGA GTCCTGGGAC GAGTGGCCTG ACATCGAGGA GGATATCGCC CTGATCAAGA GCGAAGAGGG CGAGAAAATG GTGCTTGAGA ATAACTTCTT CGTCGAGACC ATGCTCCCAA GCAAGATCAT GCGGAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG CCATTCAAGG AGAAGGGCGA GGTTAGACGG CCTACCCTCT CCTGGCCTCG CGAGATCCCT CTCGTTAAGG GAGGCAAGCC CGACGTCGTC CAGATTGTCC GCAACTACAA CGCCTACCTT CGGGCCAGCG ACGATCTGCC TAAGATGTTC ATCGAGTCCG ACCCTGGGTT CTTTTCCAAC GCTATTGTCG AGGGAGCTAA GAAGTTCCCT AACACCGAGT TCGTGAAGGT GAAGGGCCTC CACTTCAGCC AGGAGGACGC TCCAGATGAA ATGGGTAAGT ACATCAAGAG CTTCGTGGAG CGCGTGCTGA AGAACGAGCA GACCGGTGGT GGGAGCGGAG GTGGCGGATC AGGTGGCGGA GGCTCCGGAG GGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAATC GATAGCGATA AGGATCCTCT TTGCGCTTGC GTTTTCCCTT GTCCAGATAG CCCAGTAGCT GACATTCATC CGGGGTCAGC ACCGTTTCTG CGGACTGGCT TTCTACGTAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTCAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACTATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAATTC GAAATGACCG ACCAAGCGAC GCCCAACCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATTT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTATAGTCC GGAAATACAG GAACGCACGC TGGATGGCCC TTCGCTGGGA TGGTGAAACC ATGAAAAATG GCAGCTTCAG TGGATTAAGT GGGGGTAATG TGGCCTGTAC CCTCTGGTTG CATAGGTATT CATACGGTTA AAATTTATCA GGCGCGATTG CGGCAGTTTT TCGGGTGGTT TGTTGCCATT TTTACCTGTC TGCTGCCGTG ATCGCGCTGA ACGCGTTTTA GCGGTGCGTA CAATTAAGGG ATTATGGTAA ATCCACTTAC TGTCTGCCCT CGTAGCCATC GAGATAAACC GCAGTACTCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTACCGG GTAGGGGAGG CGCTTTTCCC AAGGCAGTCT GGAGCATGCG CTTTAGCAGC CCCGCTGGGC ACTTGGCGCT ACACAAGTGG CCTCTGGCCT CGCACACATT CCACATCCAC CGGTAGGCGC CAACCGGCTC CGTTCTTTGG TGGCCCCTTC GCGCCACCTT CTACTCCTCC CCTAGTCAGG AAGTTCCCCC CCGCCCCGCA GCTCGCGTCG TGCAGGACGT GACAAATGGA AGTAGCACGT CTCACTAGTC TCGTGCAGAT GGACAGCACC GCTGAGCAAT GGAAGCGGGT AGGCCTTTGG GGCAGCGGCC AATAGCAGCT TTGCTCCTTC GCTTTCTGGG CTCAGAGGCT GGGAAGGGGT GGGTCCGGGG GCGGGCTCAG GGGCGGGCTC AGGGGCGGGG CGGGCGCCCG AAGGTCCTCC GGAGGCCCGG CATTCTGCAC GCTTCAAAAG CGCACGTCTG CCGCGCTGTT CTCCTCTTCC TCATCTCCGG GCCTTTCGAC CTGCAGCCCA AGCTTGGCAA TCCGGTACTG TTGGTAAAGC CACCATGGAA GATGCCAAAA ACATTAAGAA GGGCCCAGCG CCATTCTACC CACTCGAAGA CGGGACCGCC GGCGAGCAGC TGCACAAAGC CATGAAGCGC TACGCCCTGG TGCCCGGCAC CATCGCCTTT ACCGACGCAC ATATCGAGGT GGACATTACC TACGCCGAGT ACTTCGAGAT GAGCGTTCGG CTGGCAGAAG CTATGAAGCG CTATGGGCTG AATACAAACC ATCGGATCGT GGTGTGCAGC GAGAATAGCT TGCAGTTCTT CATGCCCGTG TTGGGTGCCC TGTTCATCGG TGTGGCTGTG GCCCCAGCTA ACGACATCTA CAACGAGCGC GAGCTGCTGA ACAGCATGGG CATCAGCCAG CCCACCGTCG TATTCGTGAG CAAGAAAGGG CTGCAAAAGA TCCTCAACGT GCAAAAGAAG CTACCGATCA TACAAAAGAT CATCATCATG GATAGCAAGA CCGACTACCA GGGCTTCCAA AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCGGCT TCAACGAGTA CGACTTCGTG CCCGAGAGCT TCGACCGGGA CAAAACCATC GCCCTGATCA TGAACAGTAG TGGCAGTACC GGATTGCCCA AGGGCGTAGC CCTACCGCAC CGCACCGCTT GTGTCCGATT CAGTCATGCC CGCGACCCCA TCTTCGGCAA CCAGATCATC CCCGACACCG CTATCCTCAG CGTGGTGCCA TTTCACCACG GCTTCGGCAT GTTCACCACG CTGGGCTACT TGATCTGCGG CTTTCGGGTC GTGCTCATGT ACCGCTTCGA GGAGGAGCTA TTCTTGCGCA GCTTGCAAGA CTATAAGATT CAATCTGCCC TGCTGGTGCC CACACTATTT AGCTTCTTCG CTAAGAGCAC TCTCATCGAC AAGTACGACC TAAGCAACTT GCACGAGATC GCCAGCGGCG GGGCGCCGCT CAGCAAGGAG GTAGGTGAGG CCGTGGCCAA ACGCTTCCAC CTACCAGGCA TCCGCCAGGG CTACGGCCTG ACAGAAACAA CCAGCGCCAT TCTGATCACC CCCGAAGGGG ACGACAAGCC TGGCGCAGTA GGCAAGGTGG TGCCCTTCTT CGAGGCTAAG GTGGTGGACT TGGACACCGG TAAGACACTG GGTGTGAACC AGCGCGGCGA GCTGTGCGTC CGTGGCCCCA TGATCATGAG CGGCTACGTT AACAACCCCG AGGCTACAAA CGCTCTCATC GACAAGGACG GCTGGCTGCA CAGCGGCGAC ATCGCCTACT GGGACGAGGA CGAGCACTTC TTCATCGTGG ACCGGCTGAA GAGCCTGATC AAATACAAGG GCTACCAGGT AGCCCCAGCC GAACTGGAGA GCATCCTGCT GCAACACCCC AACATCTTCG ACGCCGGGGT CGCCGGCCTG CCCGACGACG ATGCCGGCGA GCTGCCCGCC GCAGTCGTCG TGCTGGAACA CGGTAAAACC ATGACCGAGA AGGAGATCGT GGACTATGTG GCCAGCCAGG TTACAACCGC CAAGAAGCTG CGCGGTGGTG TTGTGTTCGT GGACGAGGTG CCTAAAGGAC TGACCGGCAA GTTGGACGCC CGCAAGATCC GCGAGATTCT CATTAAGGCC AAGAAGGGCG GCAAGATCGC CGTGTAATTC TAGTTGTTTA AACGAGCTCG CTAGCTGGCC TTGTTGGCCA TTCTGGCACT CATTGGCACT TCATCCTCCT TTGTTGGGCT ATCCTGTACT CAGTAGGATA TTTGGGAACA TTCCTGGCCT CCATCTGCAA GATGCCAACA GTACAAGTCA ACCTCACAAC ACCCACCAGG TGTGAGAAAA ATAACTAGAT ATTGCTACAT GTTCTTTGGA GGGACTCTCC TGTAACGTTA TTTCCCCCAT CCCCAGACAA GGTTTCTCAG TTCTCGCTGT CCTGGAACTC ACTTTGTAGA CCAGATTGGT CTCCCAATTC AGAGATCTGC CTGCCTCTGC CTCCTGAGTG CTGGGAATAA AGGCATGTGC CACCACCACC CAGCTTCCTG CTGTAAGTTT ATATGCCTTA CTTCTCCTGT CACACAGGCT AGGCCATCAT TAACAGCATG GAACATGCAC AGTGGAAGGG AATTGGAGCA GGGAAGGTTC CAGGCTTTCC TGAAGGAGCC TGCATAGTCT TTAAAAAATG TTTTCTTTTT AACTTCTAAT GCTGTTTTAA GAAGACAGAA TTAGAAACTG CTTCTAGTGG AGTTTTAAGG AGAATAAAAT AGCAAGGGAG GCCAGAGCTG GTTTTGGAAT TGCAGTAGCA ATCAGGCTCG AGTCTAGAGT CGACCTGCAG G
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