pBBR1-Lac-T3-hslO(pseudomonas)
$113.00
In stock
SKU
PL53734
100μg liquid plasmid(Instantly):$156
| name | pBBR1-Lac-T3-hslO(pseudomonas) | another name | apBBR1-Lac-T3-hslO(pseudomonas) |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Pseudomonas | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pBBR1 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACCTTCGGGA GCGCCTGAAG CCCGTTCTGG ACGCCCTGGG GCCGTTGAAT CGGGATATGC AGGCCAAGGC CGCCGCGATC ATCAAGGCCG TGGGCGAAAA GCTGCTGACG GAACAGCGGG AAGTCCAGCG CCAGAAACAG GCCCAGCGCC AGCAGGAACG CGGGCGCGCA CATTTCCCCG AAAAGTGCCA CCTGGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG CGATAGCTAG ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC AGCTGGGGCG CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA GTAAACTGGA TGGCTTTCTT GCCGCCAAGG ATCTGATGGC GCAGGGGATC AAGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT GGCGATTCAG GTTCATCATG CCGTTTGTGA TGGCTTCCAT GTCGGCAGAA TGCTTAATGA ATTACAACAG TTTTTATGCA TGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG CAATTAACCC TCACTAAAGG GAACAAAAGC TGGGTACCAT GACTGATTTG CCGGATACCG ACTTCACTCA ACGCTTCATC TTCGATGAGA GCGACACCCG CGGCGAGCTG GTGGCGCTGG AGCGCAGTTA CGCGGAAGTC CTCGCCAAAC ACCCCTATCC CGAGCCGGTT GCACAGCTGC TTGGCGAACT GATGGCGGCG GCATCGTTGC TGGTCGGCAC CCTGAAATTC GATGGCTTGC TGATTCTCCA GGCCCGCTCC GAAGGCCCGG TGCCGCTGCT GATGATCGAG TGCTCCAGCG AGCGCGAGAT CCGTGGCCTG GCCCGTTACG ACGCGGAGCA GATCGCCCCT GATGCCACCC TCGCCGACCT GATGCCCAAC GGCGTCCTGG CCCTGACCGT GGACCCGACC AATGGCCAGC GCTACCAGGG CATCGTCGAC CTGGACGGCA CCAACCTGGC GGAATGCTTC ACCAACTACT TCGTCATGTC GCAACAGACC GGCACCCGTT TCTGGCTCTA TGCCGACGGC CGCAGCGCCC GTGGCCTGCT CCTGCAACAA CTGCCCGCCG ACCGCCTGCG TGATCAGGAA GAGCGTGACG CCAGCTGGCA GCACCTCACT GCCCTGGCCA GCACCCTGAC CGCCGATGAG CTGCTGAGCC TGGACAACGA AACCGTGCTC CACCGCCTGT ACCACGAAGA AGCCGTGCGC CTGTTCGACG TGCAGCCGCT GCGTTTTCGC TGCAGTTGCT CGCGGGAACG GTCGGGCAAT GCGCTGGTCA GCCTGGGTCT GGAAGATGCC CAGCAACTGG TGGTCGAACA TGGCGGCAGC ATCGAGATCG ATTGCCAGTT CTGCAACGAA CGCTACCTGT TCGATGCCGC TGATATCGCG CAATTGTTCG CGGGGGCGGG CGTGGACACG CCGTCAGATA CTCGTCACTA ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC CTGCAGCCCG GGGGATCCAC TAGTTCTAGA GCGGCCGCCA CCGCGGTGGA GCTCCAATTC GCCCTATAGT GAGTCGTATT ACGCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGAAA TTGTAAGCGT TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA GGCCGACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTGCTACGC CTGAATAAGT GATAATAAGC GGATGAATGG CAGAAATTCG AAAGCAAATT CGACCCGGTC GTCGGTTCAG GGCAGGGTCG TTAAATAGCC GCTTATGTCT ATTGCTGGTT TACCGGTTTA TTGACTACCG GAAGCAGTGT GACCGTGTGC TTCTCAAATG CCTGAGGCCA GTTTGCTCAG GCTCTCCCCG TGGAGGTAAT AATTGACGAT ATGATCATTT ATTCTGCCTC CCAGAGCCTG ATAAAAACGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGAGGCGGC TACAGCCGAT AGTCTGGAAC AGCGCACTTA CGGGTTGCTG CGCAACCCAA GTGCTACCGG CGCGGCAGCG TGACCCGTGT CGGCGGCTCC AACGGCTCGC CATCGTCCAG AAAACACGGC TCATCGGGCA TCGGCAGGCG CTGCTGCCCG CGCCGTTCCC ATTCCTCCGT TTCGGTCAAG GCTGGCAGGT CTGGTTCCAT GCCCGGAATG CCGGGCTGGC TGGGCGGCTC CTCGCCGGGG CCGGTCGGTA GTTGCTGCTC GCCCGGATAC AGGGTCGGGA TGCGGCGCAG GTCGCCATGC CCCAACAGCG ATTCGTCCTG GTCGTCGTGA TCAACCACCA CGGCGGCACT GAACACCGAC AGGCGCAACT GGTCGCGGGG CTGGCCCCAC GCCACGCGGT CATTGACCAC GTAGGCCGAC ACGGTGCCGG GGCCGTTGAG CTTCACGACG GAGATCCAGC GCTCGGCCAC CAAGTCCTTG ACTGCGTATT GGACCGTCCG CAAAGAACGT CCGATGAGCT TGGAAAGTGT CTTCTGGCTG ACCACCACGG CGTTCTGGTG GCCCATCTGC GCCACGAGGT GATGCAGCAG CATTGCCGCC GTGGGTTTCC TCGCAATAAG CCCGGCCCAC GCCTCATGCG CTTTGCGTTC CGTTTGCACC CAGTGACCGG GCTTGTTCTT GGCTTGAATG CCGATTTCTC TGGACTGCGT GGCCATGCTT ATCTCCATGC GGTAGGGTGC CGCACGGTTG CGGCACCATG CGCAATCAGC TGCAACTTTT CGGCAGCGCG ACAACAATTA TGCGTTGCGT AAAAGTGGCA GTCAATTACA GATTTTCTTT AACCTACGCA ATGAGCTATT GCGGGGGGTG CCGCAATGAG CTGTTGCGTA CCCCCCTTTT TTAAGTTGTT GATTTTTAAG TCTTTCGCAT TTCGCCCTAT ATCTAGTTCT TTGGTGCCCA AAGAAGGGCA CCCCTGCGGG GTTCCCCCAC GCCTTCGGCG CGGCTCCCCC TCCGGCAAAA AGTGGCCCCT CCGGGGCTTG TTGATCGACT GCGCGGCCTT CGGCCTTGCC CAAGGTGGCG CTGCCCCCTT GGAACCCCCG CACTCGCCGC CGTGAGGCTC GGGGGGCAGG CGGGCGGGCT TCGCCTTCGA CTGCCCCCAC TCGCATAGGC TTGGGTCGTT CCAGGCGCGT CAAGGCCAAG CCGCTGCGCG GTCGCTGCGC GAGCCTTGAC CCGCCTTCCA CTTGGTGTCC AACCGGCAAG CGAAGCGCGC AGGCCGCAGG CCGGAGGCTT TTCCCCAGAG AAAATTAAAA AAATTGATGG GGCAAGGCCG CAGGCCGCGC AGTTGGAGCC GGTGGGTATG TGGTCGAAGG CTGGGTAGCC GGTGGGCAAT CCCTGTGGTC AAGCTCGTGG GCAGGCGCAG CCTGTCCATC AGCTTGTCCA GCAGGGTTGT CCACGGGCCG AGCGAAGCGA GCCAGCCGGT GGCCGCTCGC GGCCATCGTC CACATATCCA CGGGCTGGCA AGGGAGCGCA GCGACCGCGC AGGGCGAAGC CCGGAGAGCA AGCCCGTAGG GCGCCGCAGC CGCCGTAGGC GGTCACGACT TTGCGAAGCA AAGTCTAGTG AGTATACTCA AGCATTGAGT GGCCCGCCGG AGGCACCGCC TTGCGCTGCC CCCGTCGAGC CGGTTGGACA CCAAAAGGGA GGGGCAGGCA TGGCGGCATA CGCGATCATG CGATGCAAGA AGCTGGCGAA AATGGGCAAC GTGGCGGCCA GTCTCAAGCA CGCCTACCGC GAGCGCGAGA CGCCCAACGC TGACGCCAGC AGGACGCCAG AGAACGAGCA CTGGGCGGCC AGCAGCACCG ATGAAGCGAT GGGCCGACTG CGCGAGTTGC TGCCAGAGAA GCGGCGCAAG GACGCTGTGT TGGCGGTCGA GTACGTCATG ACGGCCAGCC CGGAATGGTG GAAGTCGGCC AGCCAAGAAC AGCAGGCGGC GTTCTTCGAG AAGGCGCACA AGTGGCTGGC GGACAAGTAC GGGGCGGATC GCATCGTGAC GGCCAGCATC CACCGTGACG AAACCAGCCC GCACATGACC GCGTTCGTGG TGCCGCTGAC GCAGGACGGC AGGCTGTCGG CCAAGGAGTT CATCGGCAAC AAAGCGCAGA TGACCCGCGA CCAGACCACG TTTGCGGCCG CTGTGGCCGA TCTAGGGCTG CAACGGGGCA TCGAGGGCAG CAAGGCACGT CACACGCGCA TTCAGGCGTT CTACGAGGCC CTGGAGCGGC CACCAGTGGG CCACGTCACC ATCAGCCCGC AAGCGGTCGA GCCACGCGCC TATGCACCGC AGGGATTGGC CGAAAAGCTG GGAATCTCAA AGCGCGTTGA GACGCCGGAA GCCGTGGCCG ACCGGCTGAC AAAAGCGGTT CGGCAGGGGT ATGAGCCTGC CCTACAGGCC GCCGCAGGAG CGCGTGAGAT GCGCAAGAAG GCCGATCAAG CCCAAGAGAC GGCCCGAG
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