pUT-mini-Tn5-Gm-Tac-RBS-EGFP-SacB
$128.00
In stock
SKU
PL53519
100μg liquid plasmid(Instantly):$171
| name | pUT-mini-Tn5-Gm-Tac-RBS-EGFP-SacB | another name | apUT-mini-Tn5-Gm-Tac-RBS-EGFP-SacB |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | Tac | Replicon | R6K |
| Competence | S17-1λpir | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCCTTTTT GTCCGGTGTT GGGTTGAAGG TGAAGCCGGT CGGGGCCGCA GCGGGGGCCG GCTTTTCAGC CTTGCCCCCC TGCTTCGGCC GCCGTGGCTC CGGCGTCTTG GGTGCCGGCG CGGGTTCCGC AGCCTTGGCC TGCGGTGCGG GCACATCGGC GGGCTTGGCC TTGATGTGCC GCCTGGCGTG CGAGCGGAAC GTCTCGTAGG AGAACTTGAC CTTCCCCGTT TCCCGCATGT GCTCCCAAAT GGTGACGAGC GCATAGCCGG ACGCTAACGC CGCCTCGACA TCCGCCCTCA CCGCCAGGAA CGCAACCGCA GCCTCATCAC GCCGGCGCTT CTTGGCCGCG CGGGATTCAA CCCACTCGGC CAGCTCGTCG GTGTAGCTCT TTGGCATCGT CTCTCGCCTG TCCCCTCAGT TCAGTAATTT CCTGCATTTG CCTGTTTCCA GTCGGTAGAT ATTCCACAAA ACAGCAGGGA AGCAGCGCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GGCTACCGCC GGCGTAACAG ATGAGGGCAA GCGGATGGCT GATGAAACCA AGCCAACCAG GAAGGGCAGC CCACCTATCA AGGTGTACTG CCTTCCAGAC GAACGAAGAG CGATTGAGGA AAAGGCGGCG GCGGCCGGCA TGAGCCTGTC GGCCTACCTG CTGGCCGTCG GCCAGGGCTA CAAAATCACG GGCGTCGTGG ACTATGAGCA CGTCCGCGAG CTGGCCCGCA TCAATGGCGA CCTGGGCCGC CTGGGCGGCC TGCTGAAACT CTGGCTCACC GACGACCCGC GCACGGCGCG GTTCGGTGAT GCCACGATCC TCGCCCTGCT GGCGAAGATC GAAGAGAAGC AGGACGAGCT TGGCAAGGTC ATGATGGGCG TGGTCCGCCC GAGGGCAGAG CCATGACTTT TTTAGCCGCT AAAACGGCCG GGGGGTGCGC GTGATTGCCA AGCACGTCCC CATGCGCTCC ATCAAGAAGA GCGACTTCGC GGAGCTGGTG AAGTACATCA CCGACGAGCA AGGCAAGACC GAGCGCCTGG GTCACGTGCG CGTCACGAAC TGCGAGGCAA ACACCCTGCC CGCTGTCATG GCCGAGGTGA TGGCGACCCA GCACGGCAAC ACCCGTTCCG AGGCCGACAA GACCTATCAC CTGCTGGTTA GCTTCCGCGC GGGAGAGAAG CCCGACGCGG AGACGTTGCG CGCGATTGAG GACCGCATCT GCGCTGGGCT TGGCTTCGCC GAGCATCAGC GCGTCAGTGC CGTGCATCAC GACACCGACA ACCTGCACAT CCATATCGCC ATCAACAAGA TTCACCCGAC CCGAAACACC ATCCATGAGC CGTATCGGGC CTACCGCGCC CTCGCTGACC TCTGCGCGAC GCTCGAACGG GACTACGGGC TTGAGCGTGA CAATCACGAA ACGCGGCAGC GCGTTTCCGA GAACCGCGCG AACGACATGG AGCGGCACGC GGGCGTGGAA AGCCTGGTCG GCTGGATCCG GCCACGATGC GTCCGGCGTA GAGGATCTGA AGATCAGCAG TTCAACCTGT TGATAGTACG TACTAAGCTC TCATGTTTCA CGTACTAAGC TCTCATGTTT AACGTACTAA GCTCTCATGT TTAACGAACT AAACCCTCAT GGCTAACGTA CTAAGCTCTC ATGGCTAACG TACTAAGCTC TCATGTTTCA CGTACTAAGC TCTCATGTTT GAACAATAAA ATTAATATAA ATCAGCAACT TAAATAGCCT CTAAGGTTTT AAGTTTTATA AGAAAAAAAA GAATATATAA GGCTTTTAAA GCTTTTAAGG TTTAACGGTT GTGGACAACA AGCCAGGGAT GTAACGCACT GAGAAGCCCT TAGAGCCTCT CAAAGCAATT TTGAGTGACA CAGGAACACT TAACGGCTGA CATGGGAATT CCACATGTGG AATTCCACAT GTGGAATTGT GAGCGGATAA CAATTTGTGG AATTCCCGGG AGAGCTCCTG ACTCTTATAC ACAAGTTCGG CCACGGCCTA GGCGGCCGCT TGACAATTAA TCATCGGCTC GTATAATGCC TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATACCG GTATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AGGGAGCTCC AAATAAAACG AAAAGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATGCGG CCGCAGCTTG TCGACGAATT CCCGGGGATC CGGTGATTGA TTGAGCAAGC TTTATGCTTG TAAACCGTTT TGTGAAAAAA TTTTTAAAAT AAAAAAGGGG ACCTCTAGGG TCCCCAATTA ATTAGTAATA TAATCTATTA AAGGTCATTC AAAAGGTCAT CCACCGGATC AATTCCCCTG CTCACACAGA CTGGGTACCA AGCTCTCGGT AACATCAAGG CCCGATCCTT GGAGCCCTTG CCCTCCCGAC GATGATCGTG CCGTGATCGA AATCCAGATC CTTGACCCAC AGTTGCAAAC CCTCACTGAT CCGTCCCGTC CGGGTCGACC AAAGCGGCCA TCGTGCCTCC CCACTCCTGC AGTTCGGGGG CATGGATGCG CGGATAGCCG CTGCTGGTTT CCTGGATGCC GACGGATTTG CACTGCCGGT AGAACTCCGC GAGGTCGTCC AGCCTCAGGC AGCAGCTGAA CCAACTCGCG AGGGGATCGA GCCCGGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTTA GGTGGCGGTA CTTGGGTCGA TATCAAAGTG CATCACTTCT TCCCGTATGC CCAACTTTGT ATAGAGAGCC ACTGCGGGAT CGTCACCGTA ATCTGCTTGC ACGTAGATCA CATAAGCACC AAGCGCGTTG GCCTCATGCT TGAGGAGATT GATGAGCGCG GTGGCAATGC CCTGCCTCCG GTGCTCGCCG GAGACTGCGA GATCATAGAT ATAGATCTCA CTACGCGGCT GCTCAAACCT GGGCAGAACG TAAGCCGCGA GAGCGCCAAC AACCGCTTCT TGGTCGAAGG CAGCAAGCGC GATGAATGTC TTACTACGGA GCAAGTTCCC GAGGTAATCG GAGTCCGGCT GATGTTGGGA GTAGGTGGCT ACGTCTCCGA ACTCACGACC GAAAAGATCA AGAGCAGCCC GCATGGATTT GACTTGGTCA GGGCCGAGCC TACATGTGCG AATGATGCCC ATACTTGAGC CACCTAACTT TGTTTTAGGG CGACTGCCCT GCTGCGTAAC ATCGTTGCTG CTGCGTAACA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CAGATCTTGA TCCCCTGCGC CATCAGATCC TTGGCGGCAA GAAAGCCATC CAGTTTACTT TGCAGGGCTT CCCAACCTTA CCAGAGGGCG CCCCAGCTGG CAATTCCGGT TCGCTTGCTG TCCATAAAAC CGCCCAGTCT AGCTATCGTC ATGTAAGCCC ACTGCAAGCT ACCTCTTTCT CTTTGCGCTT GCGTTTTCCC TTGTCCAGAT AGCCCAGTAG CTGACATTCA TCCGGGGTAG CACCGTTTCT GCGGACTGGC TTTCTACGTG TTCCGCTTCC TTTAGCAGCC CTTGCGCCCT GATGCTTGCG GCAGCGTGAA GCTCGCGCAG ATCAGTTGGT TGATTTTGTC CACTACGTGA AAGGCGAATC ACCAAGGTAG TCGGCAAATA ATGTCTAACA ATTCGTTCAA GCCGACGCCG CTTCGCGGCG CGGTTAACTC AAGCGTTAGA TGCACTAAGC ACATAATTGC TCACAGCCAA ACTATCAGGT CAAGTCGCTT TTATTATTTT TAAGCGTGCA TAATAAGCCC TACACAAAAT TGGGAGATAT ATCATGAAAG GCGCTTTTCT TGTTATCGCA ATAGTTGGGC GAAGTAATCG CAACATCCGC ATTAACAATC TAGCGAGGGC TTGGTAAGGT GATCCGGTGG ATGACCTTTT GAATGACCTT TAATAGATTA TATTACTAAT TAATTGGGGA CCCTAGAGGT CCCCTTTTTT ATTTTAAAAA TTTTTTCACA AAACGGTTTA CAAGCATAAA GCTTGCTCAA TCAATCACCG GATCCCCGGG AATTCGGCCT AGGTGGCCAG ATCTGATCAA GAGACAGAAA CGCAAAAGAA AATGCCGATA TTAATACGAC TCACTATAGG GGAATTGTGA GCGGATAACA ATTCCCCTCT AGAAATAATT TTGTTAACTT TAAGAAGGAG ATATACATAT GATTACCAGT GCACTGCATC GTGCGGCGGA TTGGGCGAAA AGCGTGTTTT CTAGTGCTGC GCTGGGTGAT CCGCGTCGTA CCGCGCGTCT GGTGAATGTT GCGGCGCAAC TGGCCAAATA TAGCGGCAAA AGCATTACCA TTAGCAGCGA AGGCAGCAAA GCCATGCAGG AAGGCGCGTA TCGTTTTATT CGTAATCCGA ACGTGAGCGC GGAAGCGATT CGTAAAGCGG GTGCCATGCA GACCGTGAAA CTGGCCCAGG AATTTCCGGA ACTGCTGGCA ATTGAAGATA CCACCTCTCT GAGCTATCGT CATCAGGTGG CGGAAGAACT GGGCAAACTG GGTAGCATTC AGGATAAAAG CCGTGGTTGG TGGGTGCATA GCGTGCTGCT GCTGGAAGCG ACCACCTTTC GTACCGTGGG CCTGCTGCAT CAAGAATGGT GGATGCGTCC GGATGATCCG GCGGATGCGG ATGAAAAAGA AAGCGGCAAA TGGCTGGCCG CTGCTGCAAC TTCGCGTCTG AGAATGGGCA GCATGATGAG CAACGTGATT GCGGTGTGCG ATCGTGAAGC GGATATTCAT GCGTATCTGC AAGATAAACT GGCCCATAAC GAACGTTTTG TGGTGCGTAG CAAACATCCG CGTAAAGATG TGGAAAGCGG CCTGTATCTG TATGATCACC TGAAAAACCA GCCGGAACTG GGCGGCTATC AGATTAGCAT TCCGCAGAAA GGCGTGGTGG ATAAACGTGG CAAACGTAAA AACCGTCCGG CGCGTAAAGC GAGCCTGAGC CTGCGTAGCG GCCGTATTAC CCTGAAACAG GGCAACATTA CCCTGAACGC GGTGCTGGCC GAAGAAATTA ATCCGCCGAA AGGCGAAACC CCGCTGAAAT GGCTGCTGCT GACCAGCGAG CCGGTGGAAA GTCTGGCCCA AGCGCTGCGT GTGATTGATA TTTATACCCA TCGTTGGCGC ATTGAAGAAT TTCACAAAGC GTGGAAAACG GGTGCGGGTG CGGAACGTCA GCGTATGGAA GAACCGGATA ACCTGGAACG TATGGTGAGC ATTCTGAGCT TTGTGGCGGT GCGTCTGCTG CAACTGCGTG AATCTTTTAC TCCGCCGCAA GCACTGCGTG CGCAGGGCCT GCTGAAAGAA GCGGAACACG TTGAAAGCCA GAGCGCGGAA ACCGTGCTGA CCCCGGATGA ATGCCAACTG CTGGGCTATC TGGATAAAGG CAAACGCAAA CGCAAAGAAA AAGCGGGCAG CCTGCAATGG GCGTATATGG CGATTGCGCG TCTGGGCGGC TTTATGGATA GCAAACGTAC CGGCATTGCG AGCTGGGGTG CGCTGTGGGA AGGTTGGGAA GCGCTGCAAA GCAAACTGGA TGGCTTTCTG GCCGCGAAAG ACCTGATGGC GCAGGGCATT AAAATCTAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGACG CGTGCTATCG TCATGTAAGC CCACTGATCG GCATTTTCTT TTGCGTTTTT ATTTGTTAAC TGTTAATTGT CCTTGTTCAA GGATGCTGTC TTTGACAACA GATGTTTTCT TGCCTTTGAT GTTCAGCAGG AAGCTCGGCG CAAACGTTGA TTGTTTGTCT GCGTAGAATC CTCTGTTTGT CATATAGCTT GTAATCACGA CATTGTTTCC TTTCGCTTGA GGTACAGCGA AGTGTGAGTA AGTAAAGGTT ACATCGTTAG GATCAAGATC CATTTTTAAC ACAAGGCCAG TTTTGTTCAG CGGCTTGTAT GGGCCAGTTA AAGAATTAGA AACATAACCA AGCATGTAAA TATCGTTAGA CGTAATGCCG TCAATCGTCA TTTTTGATCC GCGGGAGTCA GTGAACAGGT ACCATTTGCC GTTCATTTTA AAGACGTTCG CGCGTTCAAT TTCATCTGTT ACTGTGTTAG ATGCAATCAG CGGTTTCATC ACTTTTTTCA GTGTGTAATC ATCGTTTAGC TCAATCATAC CGAGAGCGCC GTTTGCTAAC TCAGCCGTGC GTTTTTTATC GCTTTGCAGA AGTTTTTGAC TTTCTTGACG GAAGAATGAT GTGCTTTTGC CATAGTATGC TTTGTTAAAT AAAGATTCTT CGCCTTGGTA GCCATCTTCA GTTCCAGTGT TTGCTTCAAA TACTAAGTAT TTGTGGCCTT TATCTTCTAC GTAGTGAGGA TCTCTCAGCG TATGGTTGTC GCCTGAGCTG TAGTTGCCTT CATCGATGAA CTGCTGTACA TTTTGATACG TTTTTCCGTC ACCGTCAAAG ATTGATTTAT AATCCTCTAC ACCGTTGATG TTCAAAGAGC TGTCTGATGC TGATACGTTA ACTTGTGCAG TTGTCAGTGT TTGTTTGCCG TAATGTTTAC CGGAGAAATC AGTGTAGAAT AAACGGATTT TTCCGTCAGA TGTAAATGTG GCTGAACCTG ACCATTCTTG TGTTTGGTCT TTTAGGATAG AATCATTTGC ATCGAATTTG TCGCTGTCTT TAAAGACGCG GCCAGCGTTT TTCCAGCTGT CAATAGAAGT TTCGCCGACT TTTTGATAGA ACATGTAAAT CGATGTGTCA TCCGCATTTT TAGGATCTCC GGCTAATGCA AAGACGATGT GGTAGCCGTG ATAGTTTGCG ACAGTGCCGT CAGCGTTTTG TAATGGCCAG CTGTCCCAAA CGTCCAGGCC TTTTGCAGAA GAGATATTTT TAATTGTGGA CGAATCAAAT TCAGAAACTT GATATTTTTC ATTTTTTTGC TGTTCAGGGA TTTGCAGCAT ATCATGGCGT GTAATATGGG AAATGCCGTA TGTTTCCTTA TATGGCTTTT GGTTCGTTTC TTTCGCAAAC GCTTGAGTTG CGCCTCCTGC CAGCAGTGCG GTAGTAAAGG TTAATACTGT TGCTTGTTTT GCAAACTTTT TGATGTTCAT CGTTCATGTC TCCTTTTTTA TGTACTGTGT TAGCGGTCTG CTTCTTCCAG CCCTCCTGTT TGAAGATGGC AAGTTAGTTA CGCACAATAA AAAAAGACCT AAAATATGTA AGGGGTGACG CCAAAGTATA CACTTTGCCC TTTACACATT TTAGGTCTTG CCTGCTTTAT CAGTAACAAA CCCGCGCGAT TTACTTTTCG ACCTCATTCT ATTAGACTCT CGTTTGGATT GCAACTGGTC TATTTTCCTC TTTTGTTTGA TAGAAAATCA TAAAAGGATT TGCAGACTAC GGGCCTAAAG AACTAAAAAA TCTATCTGTT TCTTTTCATT CTCTGTATTT TTTATAGTTT CTGTTGCATG GGCATAAAGT TGCCTTTTTA ATCACAATTC AGAAAATATC ATAATATCTC ATTTCACTAA ATAATAGTGA ACGGCAGGTA TATGTGGGTT ACCGACTGCG GCCTGAGTTT TTTAAGTGAC GTAAAATCGT GTTGAGGCCA ACGCCCATAA TGCGGGCTGT TGCCCGGCAT CCAACGCCAT TCATGGCCAT ATCAATGATT TTCTGGTGCG TACCGGGTTG AGAAGCGGTG TAAGTGAACT GCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCA
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