pCMV-TRPA1(human)-3×FLAG-SV40-Neo
$99.00
In stock
SKU
PL53500
NM_007332.3;ANKTM1; FEPS; FEPS1
100μg liquid plasmid(Instantly):$120
| name | pCMV-TRPA1(human)-3×FLAG-SV40-Neo | another name | apCMV-TRPA1(human)-3×FLAG-SV40-Neo |
| Insert Size (bp) | 3360bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCGCCACC ATGAAGCGCA GCCTGAGGAA GATGTGGCGC CCTGGAGAAA AGAAGGAGCC CCAGGGCGTT GTCTATGAGG ATGTGCCGGA CGACACGGAG GATTTCAAGG AATCGCTTAA GGTGGTTTTT GAAGGAAGTG CATATGGATT ACAAAACTTT AATAAGCAAA AGAAATTAAA AAGATGTGAC GATATGGACA CCTTCTTCTT GCATTATGCT GCAGCAGAAG GCCAAATTGA GCTAATGGAG AAGATCACCA GAGATTCCTC TTTGGAAGTG CTGCATGAAA TGGATGATTA TGGAAATACC CCTCTGCATT GTGCTGTAGA AAAAAACCAA ATTGAAAGCG TTAAGTTTCT TCTCAGCAGA GGAGCAAACC CAAATCTCCG AAACTTCAAC ATGATGGCTC CTCTCCACAT AGCTGTGCAG GGCATGAATA ATGAGGTGAT GAAGGTCTTG CTTGAGCATA GAACTATTGA TGTTAATTTG GAAGGAGAAA ATGGAAACAC AGCTGTGATC ATTGCGTGCA CCACAAATAA TAGCGAAGCA TTGCAGATTT TGCTTAAAAA AGGAGCTAAG CCATGTAAAT CAAATAAATG GGGATGTTTC CCTATTCACC AAGCTGCATT TTCAGGTTCC AAAGAATGCA TGGAAATAAT ACTAAGGTTT GGTGAAGAGC ATGGGTACAG TAGACAGTTG CACATTAACT TTATGAATAA TGGGAAAGCC ACCCCTCTCC ACCTGGCTGT GCAAAATGGT GACTTGGAAA TGATCAAAAT GTGCCTGGAC AATGGTGCAC AAATAGACCC AGTGGAGAAG GGAAGGTGCA CAGCCATTCA TTTTGCTGCC ACCCAGGGAG CCACTGAGAT TGTTAAACTG ATGATATCGT CCTATTCTGG TAGCGTGGAT ATTGTTAACA CAACCGATGG ATGTCATGAG ACCATGCTTC ACAGAGCTTC ATTGTTTGAT CACCATGAGC TAGCAGACTA TTTAATTTCA GTGGGAGCAG ATATTAATAA GATCGATTCT GAAGGACGCT CTCCACTTAT ATTAGCAACT GCTTCTGCAT CTTGGAATAT TGTAAATTTG CTACTCTCTA AAGGTGCCCA AGTAGACATA AAAGATAATT TTGGACGTAA TTTTCTGCAT TTAACTGTAC AGCAACCTTA TGGATTAAAA AATCTGCGAC CTGAATTTAT GCAGATGCAA CAGATCAAAG AGCTGGTAAT GGATGAAGAC AACGATGGGT GTACTCCTCT ACATTATGCA TGTAGACAGG GGGGCCCTGG TTCTGTAAAT AACCTACTTG GCTTTAATGT GTCCATTCAT TCCAAAAGCA AAGATAAGAA ATCACCTCTG CATTTTGCAG CCAGTTATGG GCGTATCAAT ACCTGTCAGA GGCTCCTACA AGACATAAGT GATACGAGGC TTCTGAATGA AGGTGACCTT CATGGAATGA CTCCTCTCCA TCTGGCAGCA AAGAATGGAC ATGATAAAGT AGTTCAGCTT CTTCTGAAAA AAGGTGCATT GTTTCTCAGT GACCACAATG GCTGGACAGC TTTGCATCAT GCGTCCATGG GCGGGTACAC TCAGACCATG AAGGTCATTC TTGATACTAA TTTGAAGTGC ACAGATCGCC TGGATGAAGA CGGGAACACT GCACTTCACT TTGCTGCAAG GGAAGGCCAC GCCAAAGCCG TTGCGCTTCT TCTGAGCCAC AATGCTGACA TAGTCCTGAA CAAGCAGCAG GCCTCCTTTT TGCACCTTGC ACTTCACAAT AAGAGGAAGG AGGTTGTTCT TACGATCATC AGGAGCAAAA GATGGGATGA ATGTCTTAAG ATTTTCAGTC ATAATTCTCC AGGCAATAAA TGTCCAATTA CAGAAATGAT AGAATACCTC CCTGAATGCA TGAAGGTACT TTTAGATTTC TGCATGTTGC ATTCCACAGA AGACAAGTCC TGCCGAGACT ATTATATCGA GTATAATTTC AAATATCTTC AATGTCCATT AGAATTCACC AAAAAAACAC CTACACAGGA TGTTATATAT GAACCGCTTA CAGCCCTCAA CGCAATGGTA CAAAATAACC GCATAGAGCT TCTCAATCAT CCTGTGTGTA AAGAATATTT ACTCATGAAA TGGTTGGCTT ATGGATTTAG AGCTCATATG ATGAATTTAG GATCTTACTG TCTTGGTCTC ATACCTATGA CCATTCTCGT TGTCAATATA AAACCAGGAA TGGCTTTCAA CTCAACTGGC ATCATCAATG AAACTAGTGA TCATTCAGAA ATACTAGATA CCACGAATTC ATATCTAATA AAAACTTGTA TGATTTTAGT GTTTTTATCA AGTATATTTG GGTATTGCAA AGAAGCGGGG CAAATTTTCC AACAGAAAAG GAATTATTTT ATGGATATAA GCAATGTTCT TGAATGGATT ATCTACACGA CGGGCATCAT TTTTGTGCTG CCCTTGTTTG TTGAAATACC AGCTCATCTG CAGTGGCAAT GTGGAGCAAT TGCTGTTTAC TTCTATTGGA TGAATTTCTT ATTGTATCTT CAAAGATTTG AAAATTGTGG AATTTTTATT GTTATGTTGG AGGTAATTTT GAAAACTTTG TTGAGGTCTA CAGTTGTATT TATCTTCCTT CTTCTGGCTT TTGGACTCAG CTTTTACATC CTCCTGAATT TACAGGATCC CTTCAGCTCT CCATTGCTTT CTATAATCCA GACCTTCAGC ATGATGCTAG GAGATATCAA TTATCGAGAG TCCTTCCTAG AACCATATCT GAGAAATGAA TTGGCACATC CAGTTCTGTC CTTTGCACAA CTTGTTTCCT TCACAATATT TGTCCCAATT GTCCTCATGA ATTTACTTAT TGGTTTGGCA GTTGGCGACA TTGCTGAGGT CCAGAAACAT GCATCATTGA AGAGGATAGC TATGCAGGTG GAACTTCATA CCAGCTTAGA GAAGAAGCTG CCACTTTGGT TTCTACGCAA AGTGGATCAG AAATCCACCA TCGTGTATCC CAACAAACCC AGATCTGGTG GGATGTTATT CCATATATTC TGTTTTTTAT TTTGCACTGG GGAAATAAGA CAAGAAATAC CAAATGCTGA TAAATCTTTA GAAATGGAAA TATTAAAGCA GAAATACCGG CTGAAGGATC TTACTTTTCT CCTGGAAAAA CAGCATGAGC TCATTAAACT GATCATTCAG AAGATGGAGA TCATCTCTGA GACAGAGGAT GATGATAGCC ATTGTTCTTT TCAAGACAGG TTTAAGAAAG AGCAGATGGA ACAAAGGAAT AGCAGATGGA ATACTGTGTT GAGAGCAGTC AAGGCAAAAA CACACCATCT TGAGCCTCTC GAGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGT AGTGAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTC
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