pCMV-CEMIP(human)-EGFP-SV40-Neo
$171.00
In stock
SKU
PL53389
NM_001293298.2;CCSP1; HYBID; KIAA1199; TMEM2L
100μg liquid plasmid(Instantly):$192
| name | pCMV-CEMIP(human)-EGFP-SV40-Neo | another name | apCMV-CEMIP(human)-EGFP-SV40-Neo |
| Insert Size (bp) | 4083bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCC ACCATGGGAG CTGCTGGGAG GCAGGACTTC CTCTTCAAGG CCATGCTGAC CATCAGCTGG CTCACTCTGA CCTGCTTCCC TGGGGCCACA TCCACAGTGG CTGCTGGGTG CCCTGACCAG AGCCCTGAGT TGCAACCCTG GAACCCTGGC CATGACCAAG ACCACCATGT GCATATCGGC CAGGGCAAGA CACTGCTGCT CACCTCTTCT GCCACGGTCT ATTCCATCCA CATCTCAGAG GGAGGCAAGC TGGTCATTAA AGACCACGAC GAGCCGATTG TTTTGCGAAC CCGGCACATC CTGATTGACA ACGGAGGAGA GCTGCATGCT GGGAGTGCCC TCTGCCCTTT CCAGGGCAAT TTCACCATCA TTTTGTATGG AAGGGCTGAT GAAGGTATTC AGCCGGATCC TTACTATGGT CTGAAGTACA TTGGGGTTGG TAAAGGAGGC GCTCTTGAGT TGCATGGACA GAAAAAGCTC TCCTGGACAT TTCTGAACAA GACCCTTCAC CCAGGTGGCA TGGCAGAAGG AGGCTATTTT TTTGAAAGGA GCTGGGGCCA CCGTGGAGTT ATTGTTCATG TCATCGACCC CAAATCAGGC ACAGTCATCC ATTCTGACCG GTTTGACACC TATAGATCCA AGAAAGAGAG TGAACGTCTG GTCCAGTATT TGAACGCGGT GCCCGATGGC AGGATCCTTT CTGTTGCAGT GAATGATGAA GGTTCTCGAA ATCTGGATGA CATGGCCAGG AAGGCGATGA CCAAATTGGG AAGCAAACAC TTCCTGCACC TTGGATTTAG ACACCCTTGG AGTTTTCTAA CTGTGAAAGG AAATCCATCA TCTTCAGTGG AAGACCATAT TGAATATCAT GGACATCGAG GCTCTGCTGC TGCCCGGGTA TTCAAATTGT TCCAGACAGA GCATGGCGAA TATTTCAATG TTTCTTTGTC CAGTGAGTGG GTTCAAGACG TGGAGTGGAC GGAGTGGTTC GATCATGATA AAGTATCTCA GACTAAAGGT GGGGAGAAAA TTTCAGACCT CTGGAAAGCT CACCCAGGAA AAATATGCAA TCGTCCCATT GATATACAGG CCACTACAAT GGATGGAGTT AACCTCAGCA CCGAGGTTGT CTACAAAAAA GGCCAGGATT ATAGGTTTGC TTGCTACGAC CGGGGCAGAG CCTGCCGGAG CTACCGTGTA CGGTTCCTCT GTGGGAAGCC TGTGAGGCCC AAACTCACAG TCACCATTGA CACCAATGTG AACAGCACCA TTCTGAACTT GGAGGATAAT GTACAGTCAT GGAAACCTGG AGATACCCTG GTCATTGCCA GTACTGATTA CTCCATGTAC CAGGCAGAAG AGTTCCAGGT GCTTCCCTGC AGATCCTGCG CCCCCAACCA GGTCAAAGTG GCAGGGAAAC CAATGTACCT GCACATCGGG GAGGAGATAG ACGGCGTGGA CATGCGGGCG GAGGTTGGGC TTCTGAGCCG GAACATCATA GTGATGGGGG AGATGGAGGA CAAATGCTAC CCCTACAGAA ACCACATCTG CAATTTCTTT GACTTCGATA CCTTTGGGGG CCACATCAAG TTTGCTCTGG GATTTAAGGC AGCACACTTG GAGGGCACGG AGCTGAAGCA TATGGGACAG CAGCTGGTGG GTCAGTACCC GATTCACTTC CACCTGGCCG GTGATGTAGA CGAAAGGGGA GGTTATGACC CACCCACATA CATCAGGGAC CTCTCCATCC ATCATACATT CTCTCGCTGC GTCACAGTCC ATGGCTCCAA TGGCTTGTTG ATCAAGGACG TTGTGGGCTA TAACTCTTTG GGCCACTGCT TCTTCACGGA AGATGGGCCG GAGGAACGCA ACACTTTTGA CCACTGTCTT GGCCTCCTTG TCAAGTCTGG AACCCTCCTC CCCTCGGACC GTGACAGCAA GATGTGCAAG ATGATCACAG AGGACTCCTA CCCGGGGTAC ATCCCCAAGC CCAGGCAAGA CTGCAATGCT GTGTCCACCT TCTGGATGGC CAATCCCAAC AACAACCTCA TCAACTGTGC CGCTGCAGGA TCTGAGGAAA CTGGATTTTG GTTTATTTTT CACCACGTAC CAACGGGCCC CTCCGTGGGA ATGTACTCCC CAGGTTATTC AGAGCACATT CCACTGGGAA AATTCTATAA CAACCGAGCA CATTCCAACT ACCGGGCTGG CATGATCATA GACAACGGAG TCAAAACCAC CGAGGCCTCT GCCAAGGACA AGCGGCCGTT CCTCTCAATC ATCTCTGCCA GATACAGCCC TCACCAGGAC GCCGACCCGC TGAAGCCCCG GGAGCCGGCC ATCATCAGAC ACTTCATTGC CTACAAGAAC CAGGACCACG GGGCCTGGCT GCGCGGCGGG GATGTGTGGC TGGACAGCTG CCGGTTTGCT GACAATGGCA TTGGCCTGAC CCTGGCCAGT GGTGGAACCT TCCCGTATGA CGACGGCTCC AAGCAAGAGA TAAAGAACAG CTTGTTTGTT GGCGAGAGTG GCAACGTGGG GACGGAAATG ATGGACAATA GGATCTGGGG CCCTGGCGGC TTGGACCATA GCGGAAGGAC CCTCCCTATA GGCCAGAATT TTCCAATTAG AGGAATTCAG TTATATGATG GCCCCATCAA CATCCAAAAC TGCACTTTCC GAAAGTTTGT GGCCCTGGAG GGCCGGCACA CCAGCGCCCT GGCCTTCCGC CTGAATAATG CCTGGCAGAG CTGCCCCCAT AACAACGTGA CCGGCATTGC CTTTGAGGAC GTTCCGATTA CTTCCAGAGT GTTCTTCGGA GAGCCTGGGC CCTGGTTCAA CCAGCTGGAC ATGGATGGGG ATAAGACATC TGTGTTCCAT GACGTCGACG GCTCCGTGTC CGAGTACCCT GGCTCCTACC TCACGAAGAA TGACAACTGG CTGGTCCGGC ACCCAGACTG CATCAATGTT CCCGACTGGA GAGGGGCCAT TTGCAGTGGG TGCTATGCAC AGATGTACAT TCAAGCCTAC AAGACCAGTA ACCTGCGAAT GAAGATCATC AAGAATGACT TCCCCAGCCA CCCTCTTTAC CTGGAGGGGG CGCTCACCAG GAGCACCCAT TACCAGCAAT ACCAACCGGT TGTCACCCTG CAGAAGGGCT ACACCATCCA CTGGGACCAG ACGGCCCCCG CCGAACTCGC CATCTGGCTC ATCAACTTCA ACAAGGGCGA CTGGATCCGA GTGGGGCTCT GCTACCCGCG AGGCACCACA TTCTCCATCC TCTCGGATGT TCACAATCGC CTGCTGAAGC AAACGTCCAA GACGGGCGTC TTCGTGAGGA CCTTGCAGAT GGACAAAGTG GAGCAGAGCT ACCCTGGCAG GAGCCACTAC TACTGGGACG AGGACTCAGG GCTGTTGTTC CTGAAGCTGA AAGCTCAGAA CGAGAGAGAG AAGTTTGCTT TCTGCTCCAT GAAAGGCTGT GAGAGGATAA AGATTAAAGC TCTGATTCCA AAGAACGCAG GCGTCAGTGA CTGCACAGCC ACAGCTTACC CCAAGTTCAC CGAGAGGGCT GTCGTAGACG TGCCGATGCC CAAGAAGCTC TTTGGTTCTC AGCTGAAAAC AAAGGACCAT TTCTTGGAGG TGAAGATGGA GAGTTCCAAG CAGCACTTCT TCCACCTCTG GAACGACTTC GCTTACATTG AAGTGGATGG GAAGAAGTAC CCCAGTTCGG AGGATGGCAT CCAGGTGGTG GTGATTGACG GGAACCAAGG GCGCGTGGTG AGCCACACGA GCTTCAGGAA CTCCATTCTG CAAGGCATAC CATGGCAGCT TTTCAACTAT GTGGCGACCA TCCCTGACAA TTCCATAGTG CTTATGGCAT CAAAGGGAAG ATACGTCTCC AGAGGCCCAT GGACCAGAGT GCTGGAAAAG CTTGGGGCAG ACAGGGGTCT CAAGTTGAAA GAGCAAATGG CATTCGTTGG CTTCAAAGGC AGCTTCCGGC CCATCTGGGT GACACTGGAC ACTGAGGATC ACAAAGCCAA AATCTTCCAA GTTGTGCCCA TCCCTGTGGT GAAGAAGAAG AAGTTGGGAG GAGGGGGAAG CGATCCACCG GTCGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAA GCGGCCGCGA CTCTAGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAAGGCGTA AATTGTAAGC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTCCTG AGGCGGAAAG AACCAGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCTAGG GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGTGTT GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCC TCAGGTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCAT GCAT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
