pCMV-LINC00330(human)
$84.00
In stock
SKU
PL53202
NR_038433.3;NCRNA00330
100μg liquid plasmid(Instantly):$105
| name | pCMV-LINC00330(human) | another name | apCMV-LINC00330(human) |
| Insert Size (bp) | 2035bp | Plasmid host | Mammary cell |
| Plasmid uses | Transcription regulation | Fragment type | ncRNA |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACTGCTAGCT TGGGATCTTT GTGAAGGAAC CTTACTTCTG TGGTGTGACA TAATTGGACA AACTACCTAC AGAGATTTAA AGCTCTAAGG TAAATATAAA ATTTTTAAGT GTATAATGTG TTAAACTAGC TGCATATGCT TGCTGCTTGA GAGTTTTGCT TACTGAGTAT GATTTATGAA AATATTATAC ACAGGAGCTA GTGATTCTAA TTGTTTGTGT ATTTTAGATT CACAGTCCCA AGGCTCATTT CAGGCCCCTC AGTCCTCACA GTCTGTTCAT GATCATAATC AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT CGATCCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGTTTACAAT TTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGCCAGG TCTTGGACTG GTGAGAACGG CTTGCTCGGC AGCTTCGATG TGTGCTGGAG GGAGAATAAA GGTCTAAGAT GTGCGATAGA GGGAAGTCGC ATTGAATTAT GTGCTGTGTA GGGATCGCTG GTATCAAATA TGTGTGCCCA CCCCTGGCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGC CATAACTTCG TATAATGTAT GCTATACGAA GTTATGGCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGAGC TTGCAATTCG CGCGTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTAGT ACGAGGCCCT TTCACTCATT AGATGCATGT CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGGACTCT AGCCTAGGCC GCGGGACGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATTAGGCCT ATTTAGGTGA CACTATAGAA CAAGTTTGTA CAAAAAAGCA GGCTGGTACC AAACAGTTGA GAACAGACTC CACCTTGTAG GACAGTGGCA TTTCTGAAAG TTCAGAACAA TTCCCCGAGC AACTTTACCT CACAATCTCA TTGGAACGGC TGCATCTGTG GGGCCCTGGG AGAGACTCAG CAGCAGACAG GGTCTCCAGA GCACACGACA AGAGGAAGAA TGCATGAAGG TCCACCGGCA GTCGAATCAG CCAAAGCACA AGACAAACAC AAAGTCTTGC TCTGTCGCCC AGGCTGGAGT GCAGTGGTGC GACCTTGGCT CACTGGTTTG GGGCATGTTC CTGGGTGTGT CTGTGAGGAG CAAGAAAGCC AGTTCAAGTC CCAAAACCTC AAAAGTAGGG AAGCCGACAG GGCAGCCTTA AGTCTGTGGC TGAAGGCCTG AGAGACCCTG GCAAATCACT GATATAAGTC CAAAAGCTGA AGAACTTGGA ATCTGATGTT GGAATCTGAT CTTGGCCCAT CCTTGAAGAA TCTGGGGGTA ATAAATACCC TGAACATTGA TGAACCCAAA GGGAAGCCAG AAGGTCAGAT GTCAGCTGTC GGGAAGAAAG GCTGGATAGG AAATAGGAAA AAGCAGGGCA AACAAGGAGC TTCAAGGACT GAAACCCTTA AGAACCACTT GACTCTGTCT CTCACCACCT CCAGCCTTGA TGACACGGTG ACATATAGAA GAACTGATGC CCTTTGCTTT GAGCTGCACA AAAACCTGGC GCTGGACTTG GAGAAGCTGA AAGACAGGAC CCGGCAGGAG CCTGAGGAGC TGCAGGCTGC TAGCCTGTAG ACCACCGTGC TGTGTTAGCC ACTGCAGCTG GGACCATTCT GACACTCAGA GCCTGGAAAT GGCTGCTCCC TCCTGCCTGC TATCCAAGTA TCCTGTGGAT ACTTGGCTTT TCTCTTGTGG GCAACACTAA CCTGAAACTC TTTGGGGAAG GGAATTGAGG AAATGTAGTT CCAAGATTAG CCAATTTAAC TCAGCATAAA CCCATCACAG GGTTTGACTA CAGAGTTATA ACGTTATTCT GTAGGCAATG AAAACAGAAG TTTTTGAAAA AGGCATCAAT TTGATGAAAT TGATATTTTG GGGAAGATTA ATCTTCTGCT GAGTAAAATA TAACAGGTTG GAGGAAGATG AAAATGGAGA GAGTAGAGTG TAATTAAAAG TTTGTTGGAG GCCGGGCACG GGATTACGGC TCACTCCTGT AATCCCAGCA CTTTGGGAGG CTGAGGTGGG CGGATCACCT GAGGTCAGGA GCTTGAGATC ATCCCGGCCA ACATAGTGAA ACCCTGTCTC TAATAAAAAT ACGAAAAATT AGCTGGGCAT GGTGGCGCAC ACCTGTAATC TCAGCTACTC GGGAGGCTGA GGCAGGAGAA TCCCTTGAAC CCAGGAGGCA GAGGTTGCAA TGAGCCAAGA TGGTGTGATT GCACTCCAGC CCAGGGGACA ATGTGAGACT CTGTCTCAAA AAAAAAAAAA AATTTGTTGT AGTAGTCCAG GTGTCCGGGT GTTGTTACCA GGGCACCCAT GATGAAATAA CTGGGAGAGT CCTTTTTTCT GACTGGTTTT CTTCAGTCCC TTTATCTACA GGCACAACTG CTGAAGAAAC CAGATGGCCT GGGATGGCAC CAGAGCTTTT TTACCCTTGA CCAGATACTA GAGGGAATTA AGACCCCACA AGTGGGCACG AACTGGAACT GGCGACCCCT AGTTGCCTTC AGATCATTAA CACATCATTA TAATGCTAAA ATTCCCTGCC ATTTTGTGAA CATGGGTTGC ATGAAGACGT AAGTTTATGA ATTGCCTCTG CACACCTAGA GTCCCACCCT GTACAGGCTA ACATTCCTCC CTACATGCAC CCCCCCACAA ACACACCCTG CCTCCCCCAG TCCTTAGAAA CCCTATGCCT GGCCGGGCGC AGTGGCTCAC GCCTGTAATC CCAGCACTTT GGGAGGCTGA GGCGGGCGGA TCACTAGGTC AGGAGATCGA GACCATCCTG GCTAACACGG TGAAACCCTG TCTTTACTAA AAATCACGCG TACCCAGCTT TCTTGTACAA AGTGGTCCCT ATAGTGAGTC GTATTATAAG CTAGGCACTG G
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