pCMV-FLAG-KDM6A(human)(1-848aa)
$84.00
In stock
SKU
PL53160
100μg liquid plasmid(Instantly):$105
| name | pCMV-FLAG-KDM6A(human)(1-848aa) | another name | apCMV-FLAG-KDM6A(human)(1-848aa) |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTGATCT ATACATTGAA TCAATATTGG CAATTAGCCA TATTAGTCAT TGGTTATATA GCATAAATCA ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA ATTGATCTAC CATGGACTAC AAAGACGATG ACGACAAGCT TATGAAATCC TGCGGAGTGT CGCTCGCTAC CGCCGCCGCT GCCGCCGCCG CTTTCGGTGA TGAGGAAAAG AAAATGGCGG CGGGAAAAGC GAGCGGCGAG AGCGAGGAGG CGTCCCCCAG CCTGACAGCC GAGGAGAGGG AGGCGCTCGG CGGACTGGAC AGCCGCCTCT TTGGGTTCGT GAGATTTCAT GAAGATGGCG CCAGGACGAA GGCCCTACTG GGCAAGGCTG TTCGCTGCTA TGAATCTCTA ATCTTAAAAG CTGAAGGAAA AGTGGAGTCT GATTTCTTTT GTCAATTAGG TCACTTCAAC CTCTTATTGG AAGATTATCC AAAAGCATTA TCTGCATACC AGAGGTACTA CAGTTTACAG TCTGACTACT GGAAGAATGC TGCCTTTTTA TATGGTCTTG GTTTGGTCTA CTTCCATTAT AATGCATTTC AGTGGGCAAT TAAAGCATTT CAGGAGGTGC TTTATGTTGA TCCCAGCTTT TGTCGAGCCA AGGAAATTCA TTTACGACTT GGGCTTATGT TCAAAGTGAA CACAGACTAT GAGTCTAGTT TAAAGCATTT TCAGTTAGCT TTGGTTGACT GTAATCCCTG CACTTTGTCC AATGCTGAAA TTCAATTTCA CATTGCCCAC TTATATGAAA CCCAGAGGAA ATATCATTCT GCAAAAGAAG CTTATGAACA ACTTTTGCAG ACAGAGAATC TTTCTGCACA AGTAAAAGCA ACTGTCTTAC AACAGTTAGG TTGGATGCAT CACACTGTAG ATCTCCTGGG AGATAAAGCC ACCAAGGAAA GCTATGCTAT TCAGTATCTC CAAAAGTCCT TGGAAGCAGA TCCTAATTCT GGCCAGTCCT GGTATTTCCT CGGAAGGTGC TATTCAAGTA TTGGGAAAGT TCAGGATGCC TTTATATCTT ACAGGCAGTC TATTGATAAA TCAGAAGCAA GTGCAGATAC ATGGTGTTCA ATAGGTGTGC TATATCAGCA GCAAAATCAG CCCATGGATG CTTTACAGGC CTATATTTGT GCTGTACAAT TGGACCATGG CCATGCTGCA GCCTGGATGG ACCTAGGCAC TCTCTATGAA TCCTGCAACC AGCCTCAGGA TGCCATTAAA TGCTACTTAA ATGCAACTAG AAGCAAAAGT TGTAGTAATA CCTCTGCACT TGCAGCACGA ATTAAGTATT TACAGGCTCA GTTGTGTAAC CTTCCACAAG GTAGTCTACA GAATAAAACT AAATTACTTC CTAGTATTGA GGAGGCGTGG AGCCTACCAA TTCCCGCAGA GCTTACCTCC AGGCAGGGTG CCATGAACAC AGCACAGCAG AATACTTCTG ACAATTGGAG TGGTGGACAT GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA TTACAGCATT TGGAACAGCT CCGCGCAAAT AGAAATAATT TAAATCCAGC ACAGAAACTG ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACCAACA GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT GGGCAGCCTT TGGCCAATGG ACCCTTTTCT GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGAA GTACAGACAC TATTTTGATA GGCAATAATC ATATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTCGGCAGGT CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC TCTGGTATTC AGAATCAGAA CGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ATATTGACGG TGCCTGAAAC AAGCAGGCAC ACTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG ATGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAC TCTGTTGCCT CTTCACCATC TTCAGCCATT TCAACAGCAA CACCTTCTCC AAAATCCACT GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACTAGGT ACCAGTCGAC TCTAGAGGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CTCGAGGAAC TGAAAAACCA GAAAGTTAAT TCCCTATAGT GAGTCGTATT AAATTCGTAA TCATGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTT
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