pCMV-TRPA1(human)-Hyg
$99.00
In stock
SKU
PL53122
100μg liquid plasmid(Instantly):$120
| name | pCMV-TRPA1(human)-Hyg | another name | apCMV-TRPA1(human)-Hyg |
| Insert Size (bp) | 3357bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GCCACCATGA AGCGCAGCCT GAGGAAGATG TGGCGCCCTG GAGAAAAGAA GGAGCCCCAG GGCGTTGTCT ATGAGGATGT GCCGGACGAC ACGGAGGATT TCAAGGAATC GCTTAAGGTG GTTTTTGAAG GAAGTGCATA TGGATTACAA AACTTTAATA AGCAAAAGAA ATTAAAAAGA TGTGACGATA TGGACACCTT CTTCTTGCAT TATGCTGCAG CAGAAGGCCA AATTGAGCTA ATGGAGAAGA TCACCAGAGA TTCCTCTTTG GAAGTGCTGC ATGAAATGGA TGATTATGGA AATACCCCTC TGCATTGTGC TGTAGAAAAA AACCAAATTG AAAGCGTTAA GTTTCTTCTC AGCAGAGGAG CAAACCCAAA TCTCCGAAAC TTCAACATGA TGGCTCCTCT CCACATAGCT GTGCAGGGCA TGAATAATGA GGTGATGAAG GTCTTGCTTG AGCATAGAAC TATTGATGTT AATTTGGAAG GAGAAAATGG AAACACAGCT GTGATCATTG CGTGCACCAC AAATAATAGC GAAGCATTGC AGATTTTGCT TAAAAAAGGA GCTAAGCCAT GTAAATCAAA TAAATGGGGA TGTTTCCCTA TTCACCAAGC TGCATTTTCA GGTTCCAAAG AATGCATGGA AATAATACTA AGGTTTGGTG AAGAGCATGG GTACAGTAGA CAGTTGCACA TTAACTTTAT GAATAATGGG AAAGCCACCC CTCTCCACCT GGCTGTGCAA AATGGTGACT TGGAAATGAT CAAAATGTGC CTGGACAATG GTGCACAAAT AGACCCAGTG GAGAAGGGAA GGTGCACAGC CATTCATTTT GCTGCCACCC AGGGAGCCAC TGAGATTGTT AAACTGATGA TATCGTCCTA TTCTGGTAGC GTGGATATTG TTAACACAAC CGATGGATGT CATGAGACCA TGCTTCACAG AGCTTCATTG TTTGATCACC ATGAGCTAGC AGACTATTTA ATTTCAGTGG GAGCAGATAT TAATAAGATC GATTCTGAAG GACGCTCTCC ACTTATATTA GCAACTGCTT CTGCATCTTG GAATATTGTA AATTTGCTAC TCTCTAAAGG TGCCCAAGTA GACATAAAAG ATAATTTTGG ACGTAATTTT CTGCATTTAA CTGTACAGCA ACCTTATGGA TTAAAAAATC TGCGACCTGA ATTTATGCAG ATGCAACAGA TCAAAGAGCT GGTAATGGAT GAAGACAACG ATGGGTGTAC TCCTCTACAT TATGCATGTA GACAGGGGGG CCCTGGTTCT GTAAATAACC TACTTGGCTT TAATGTGTCC ATTCATTCCA AAAGCAAAGA TAAGAAATCA CCTCTGCATT TTGCAGCCAG TTATGGGCGT ATCAATACCT GTCAGAGGCT CCTACAAGAC ATAAGTGATA CGAGGCTTCT GAATGAAGGT GACCTTCATG GAATGACTCC TCTCCATCTG GCAGCAAAGA ATGGACATGA TAAAGTAGTT CAGCTTCTTC TGAAAAAAGG TGCATTGTTT CTCAGTGACC ACAATGGCTG GACAGCTTTG CATCATGCGT CCATGGGCGG GTACACTCAG ACCATGAAGG TCATTCTTGA TACTAATTTG AAGTGCACAG ATCGCCTGGA TGAAGACGGG AACACTGCAC TTCACTTTGC TGCAAGGGAA GGCCACGCCA AAGCCGTTGC GCTTCTTCTG AGCCACAATG CTGACATAGT CCTGAACAAG CAGCAGGCCT CCTTTTTGCA CCTTGCACTT CACAATAAGA GGAAGGAGGT TGTTCTTACG ATCATCAGGA GCAAAAGATG GGATGAATGT CTTAAGATTT TCAGTCATAA TTCTCCAGGC AATAAATGTC CAATTACAGA AATGATAGAA TACCTCCCTG AATGCATGAA GGTACTTTTA GATTTCTGCA TGTTGCATTC CACAGAAGAC AAGTCCTGCC GAGACTATTA TATCGAGTAT AATTTCAAAT ATCTTCAATG TCCATTAGAA TTCACCAAAA AAACACCTAC ACAGGATGTT ATATATGAAC CGCTTACAGC CCTCAACGCA ATGGTACAAA ATAACCGCAT AGAGCTTCTC AATCATCCTG TGTGTAAAGA ATATTTACTC ATGAAATGGT TGGCTTATGG ATTTAGAGCT CATATGATGA ATTTAGGATC TTACTGTCTT GGTCTCATAC CTATGACCAT TCTCGTTGTC AATATAAAAC CAGGAATGGC TTTCAACTCA ACTGGCATCA TCAATGAAAC TAGTGATCAT TCAGAAATAC TAGATACCAC GAATTCATAT CTAATAAAAA CTTGTATGAT TTTAGTGTTT TTATCAAGTA TATTTGGGTA TTGCAAAGAA GCGGGGCAAA TTTTCCAACA GAAAAGGAAT TATTTTATGG ATATAAGCAA TGTTCTTGAA TGGATTATCT ACACGACGGG CATCATTTTT GTGCTGCCCT TGTTTGTTGA AATACCAGCT CATCTGCAGT GGCAATGTGG AGCAATTGCT GTTTACTTCT ATTGGATGAA TTTCTTATTG TATCTTCAAA GATTTGAAAA TTGTGGAATT TTTATTGTTA TGTTGGAGGT AATTTTGAAA ACTTTGTTGA GGTCTACAGT TGTATTTATC TTCCTTCTTC TGGCTTTTGG ACTCAGCTTT TACATCCTCC TGAATTTACA GGATCCCTTC AGCTCTCCAT TGCTTTCTAT AATCCAGACC TTCAGCATGA TGCTAGGAGA TATCAATTAT CGAGAGTCCT TCCTAGAACC ATATCTGAGA AATGAATTGG CACATCCAGT TCTGTCCTTT GCACAACTTG TTTCCTTCAC AATATTTGTC CCAATTGTCC TCATGAATTT ACTTATTGGT TTGGCAGTTG GCGACATTGC TGAGGTCCAG AAACATGCAT CATTGAAGAG GATAGCTATG CAGGTGGAAC TTCATACCAG CTTAGAGAAG AAGCTGCCAC TTTGGTTTCT ACGCAAAGTG GATCAGAAAT CCACCATCGT GTATCCCAAC AAACCCAGAT CTGGTGGGAT GTTATTCCAT ATATTCTGTT TTTTATTTTG CACTGGGGAA ATAAGACAAG AAATACCAAA TGCTGATAAA TCTTTAGAAA TGGAAATATT AAAGCAGAAA TACCGGCTGA AGGATCTTAC TTTTCTCCTG GAAAAACAGC ATGAGCTCAT TAAACTGATC ATTCAGAAGA TGGAGATCAT CTCTGAGACA GAGGATGATG ATAGCCATTG TTCTTTTCAA GACAGGTTTA AGAAAGAGCA GATGGAACAA AGGAATAGCA GATGGAATAC TGTGTTGAGA GCAGTCAAGG CAAAAACACA CCATCTTGAG CCTTAGGCGG CCGCTCGAGT CTAGAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA CCTAGAAGTT CCTATTCCGA AGTTCCTATT CTCTAGAAAG TATAGGAACT TCCTTGGCCA AAAAGCCTGA ACTCACCGCG ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG TCTCCGACCT GATGCAGCTC TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG GAGGGCGTGG ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT ATGTTTATCG GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG AATTCAGCGA GAGCCTGACC TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG ACCTGCCTGA AACCGAACTG CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA TCGCTGCGGC CGATCTTAGC CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG GTCAATACAC TACATGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT GGCAAACTGT GATGGACGAC ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA TGCTTTGGGC CGAGGACTGC CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCA ACAATGTCCT GACGGACAAT GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT TCGGGGATTC CCAATACGAG GTCGCCAACA TCTTCTTCTG GAGGCCGTGG TTGGCTTGTA TGGAGCAGCA GACGCGCTAC TTCGAGCGGA GGCATCCGGA GCTTGCAGGA TCGCCGCGGC TCCGGGCGTA TATGCTCCGC ATTGGTCTTG ACCAACTCTA TCAGAGCTTG GTTGACGGCA ATTTCGATGA TGCAGCTTGG GCGCAGGGTC GATGCGACGC AATCGTCCGA TCCGGAGCCG GGACTGTCGG GCGTACACAA ATCGCCCGCA GAAGCGCGGC CGTCTGGACC GATGGCTGTG TAGAAGTACT CGCCGATAGT GGAAACCGAC GCCCCAGCAC TCGTCCGAGG GCAAAGGAAT AGCACGTACT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTC
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