pCMV-Spike(SARS-Cov-2)
$99.00
In stock
SKU
PL53036
100μg liquid plasmid(Instantly):$120
| name | pCMV-Spike(SARS-Cov-2) | another name | apCMV-Spike(SARS-Cov-2) |
| Insert Size (bp) | 3822bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | COVID-19 | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGATCCCCTG AGGGGGCCCC CATGGGCTAG AGGATCCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGA GCTTGGCCCA TTGCATACGT TGTATCCATA TCATAATATG TACATTTATA TTGGCTCATG TCCAACATTA CCGCCATGTT GACATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCC CTCGAAGCTT ACATGTGGTA CCGAGCTCGG ATCCTGAGAA CTTCAGGGTG AGTCTATGGG ACCCTTGATG TTTTCTTTCC CCTTCTTTTC TATGGTTAAG TTCATGTCAT AGGAAGGGGA GAAGTAACAG GGTACACATA TTGACCAAAT CAGGGTAATT TTGCATTTGT AATTTTAAAA AATGCTTTCT TCTTTTAATA TACTTTTTTG TTTATCTTAT TTCTAATACT TTCCCTAATC TCTTTCTTTC AGGGCAATAA TGATACAATG TATCATGCCT CTTTGCACCA TTCTAAAGAA TAACAGTGAT AATTTCTGGG TTAAGGCAAT AGCAATATTT CTGCATATAA ATATTTCTGC ATATAAATTG TAACTGATGT AAGAGGTTTC ATATTGCTAA TAGCAGCTAC AATCCAGCTA CCATTCTGCT TTTATTTTAT GGTTGGGATA AGGCTGGATT ATTCTGAGTC CAAGCTAGGC CCTTTTGCTA ATCATGTTCA TACCTCTTAT CTTCCTCCCA CAGCTCCTGG GCAACGTGCT GGTCTGTGTG CTGGCCCATC ACTTTGGCAA AGCACGTGAG ATCTGAATTC GCCACCATGT TTGTTTTTCT TGTTTTATTG CCACTAGTCT CTAGTCAGTG TGTTAATCTT ACAACCAGAA CTCAATTACC CCCTGCATAC ACTAATTCTT TCACACGTGG TGTTTATTAC CCTGACAAAG TTTTCAGATC CTCAGTTTTA CATTCAACTC AGGACTTGTT CTTACCTTTC TTTTCCAATG TTACTTGGTT CCATGCTATA CATGTCTCTG GGACCAATGG TACTAAGAGG TTTGATAACC CTGTCCTACC ATTTAATGAT GGTGTTTATT TTGCTTCCAC TGAGAAGTCT AACATAATAA GAGGCTGGAT TTTTGGTACT ACTTTAGATT CGAAGACCCA GTCCCTACTT ATTGTTAATA ACGCTACTAA TGTTGTTATT AAAGTCTGTG AATTTCAATT TTGTAATGAT CCATTTTTGG GTGTTTATTA CCACAAAAAC AACAAAAGTT GGATGGAAAG TGAGTTCAGA GTTTATTCTA GTGCGAATAA TTGCACTTTT GAATATGTCT CTCAGCCTTT TCTTATGGAC CTTGAAGGAA AACAGGGTAA TTTCAAAAAT CTTAGGGAAT TTGTGTTTAA GAATATTGAT GGTTATTTTA AAATATATTC TAAGCACACG CCTATTAATT TAGTGCGTGA TCTCCCTCAG GGTTTTTCGG CTTTAGAACC ATTGGTAGAT TTGCCAATAG GTATTAACAT CACTAGGTTT CAAACTTTAC TTGCTTTACA TAGAAGTTAT TTGACTCCTG GTGATTCTTC TTCAGGTTGG ACAGCTGGTG CTGCAGCTTA TTATGTGGGT TATCTTCAAC CTAGGACTTT TCTATTAAAA TATAATGAAA ATGGAACCAT TACAGATGCT GTAGACTGTG CACTTGACCC TCTCTCAGAA ACAAAGTGTA CGTTGAAATC CTTCACTGTA GAAAAAGGAA TCTATCAAAC TTCTAACTTT AGAGTCCAAC CAACAGAATC TATTGTTAGA TTTCCTAATA TTACAAACTT GTGCCCTTTT GGTGAAGTTT TTAACGCCAC CAGATTTGCA TCTGTTTATG CTTGGAACAG GAAGAGAATC AGCAACTGTG TTGCTGATTA TTCTGTCCTA TATAATTCCG CATCATTTTC CACTTTTAAG TGTTATGGAG TGTCTCCTAC TAAATTAAAT GATCTCTGCT TTACTAATGT CTATGCAGAT TCATTTGTAA TTAGAGGTGA TGAAGTCAGA CAAATCGCTC CAGGGCAAAC TGGAAAGATT GCTGATTATA ATTATAAATT ACCAGATGAT TTTACAGGCT GCGTTATAGC TTGGAATTCT AACAATCTTG ATTCTAAGGT TGGTGGTAAT TATAATTACC TGTATAGATT GTTTAGGAAG TCTAATCTCA AACCTTTTGA GAGAGATATT TCAACTGAAA TCTATCAGGC CGGTAGCACA CCTTGTAATG GTGTTGAAGG TTTTAATTGT TACTTTCCTT TACAATCATA TGGTTTCCAA CCCACTAATG GTGTTGGTTA CCAACCATAC AGAGTAGTAG TACTTTCTTT TGAACTTCTA CATGCACCAG CAACTGTTTG TGGACCTAAA AAGTCTACTA ATTTGGTTAA AAACAAATGT GTCAATTTCA ACTTCAATGG TTTAACAGGC ACAGGTGTTC TTACTGAGTC TAACAAAAAG TTTCTGCCTT TCCAACAATT TGGCAGAGAC ATTGCTGACA CTACTGATGC TGTCCGTGAT CCACAGACAC TTGAGATTCT TGACATTACA CCATGTTCTT TTGGTGGTGT CAGTGTTATA ACACCAGGAA CAAATACTTC TAACCAGGTT GCTGTTCTTT ATCAGGATGT TAACTGCACA GAAGTCCCTG TTGCTATTCA TGCAGATCAA CTTACTCCTA CTTGGCGTGT TTATTCTACA GGTTCTAATG TTTTTCAAAC ACGTGCAGGC TGTTTAATAG GGGCTGAACA TGTCAACAAC TCATATGAGT GTGACATACC CATTGGTGCA GGTATATGCG CTAGTTATCA GACTCAGACT AATTCTCCTC GGCGGGCACG TAGTGTAGCT AGTCAATCCA TCATTGCCTA CACTATGTCA CTTGGTGCAG AAAATTCAGT TGCTTACTCT AATAACTCTA TTGCCATACC CACAAATTTT ACTATTAGTG TTACCACAGA AATTCTACCA GTGTCTATGA CCAAGACATC AGTAGATTGT ACAATGTACA TTTGTGGTGA TTCAACTGAA TGCAGCAATC TTTTGTTGCA ATATGGCAGT TTTTGTACAC AATTAAACCG TGCTTTAACT GGAATAGCTG TTGAACAAGA CAAAAACACC CAAGAAGTTT TTGCACAAGT CAAACAAATT TACAAAACAC CACCAATTAA AGATTTTGGT GGTTTTAATT TTTCACAAAT ATTACCAGAT CCATCAAAAC CAAGCAAGAG GTCATTTATT GAAGATCTAC TTTTCAACAA AGTGACACTT GCAGATGCTG GCTTCATCAA ACAATATGGT GATTGCCTTG GTGATATTGC TGCTAGAGAC CTCATTTGTG CACAAAAGTT TAACGGCCTT ACTGTTTTGC CACCTTTGCT CACAGATGAA ATGATTGCTC AATACACTTC TGCACTGTTA GCGGGTACAA TCACTTCTGG TTGGACCTTT GGTGCAGGTG CTGCATTACA AATACCATTT GCTATGCAAA TGGCTTATAG GTTTAATGGT ATTGGAGTTA CACAGAATGT TCTCTATGAG AACCAAAAAT TGATTGCCAA CCAATTTAAT AGTGCTATTG GCAAAATTCA AGACTCACTT TCTTCCACAG CAAGTGCACT TGGAAAACTT CAAGATGTGG TCAACCAAAA TGCACAAGCT TTAAACACGC TTGTTAAACA ACTTAGCTCC AATTTTGGTG CAATTTCAAG TGTTTTAAAT GATATCCTTT CACGTCTTGA CAAAGTTGAG GCTGAAGTGC AAATTGATAG GTTGATCACA GGCAGACTTC AAAGTTTGCA GACATATGTG ACTCAACAAT TAATTAGAGC TGCAGAAATC AGAGCTTCTG CTAATCTTGC TGCTACTAAA ATGTCAGAGT GTGTACTTGG ACAATCAAAA AGAGTTGATT TTTGTGGAAA GGGCTATCAT CTTATGTCCT TCCCTCAGTC AGCACCTCAT GGTGTAGTCT TCTTGCATGT GACTTATGTC CCTGCACAAG AAAAGAACTT CACAACTGCT CCTGCCATTT GTCATGATGG AAAAGCACAC TTTCCTCGTG AAGGTGTCTT TGTTTCAAAT GGCACACACT GGTTTGTAAC ACAAAGGAAT TTTTATGAAC CACAAATCAT TACTACAGAC AACACATTTG TGTCTGGTAA CTGTGATGTT GTAATAGGAA TTGTCAACAA CACAGTTTAT GATCCTTTGC AACCTGAATT AGACTCATTC AAGGAGGAGT TAGATAAATA TTTTAAGAAT CATACATCAC CAGATGTTGA TTTAGGTGAC ATCTCTGGCA TTAATGCTTC AGTTGTAAAC ATTCAAAAAG AAATTGACCG CCTCAATGAG GTTGCCAAGA ATTTAAATGA ATCTCTCATC GATCTCCAAG AACTTGGAAA GTATGAGCAG TATATAAAAT GGCCATGGTA CATTTGGCTA GGTTTTATAG CTGGCTTGAT TGCCATAGTA ATGGTGACAA TTATGCTTTG CTGTATGACC AGTTGCTGTA GTTGTCTCAA GGGCTGTTGT TCTTGTGGAT CCTGCTGCAA ATTTGATGAA GACGACTCTG AGCCAGTGCT CAAAGGAGTC AAATTACATT ACACATAAGA ATTCACCCCA CCAGTGCAGG CTGCCTATCA GAAAGTGGTG GCTGGTGTGG CTAATGCCCT GGCCCACAAG TTTCACTAAG CTCGCTTCCT TGCTGTCCAA TTTCTATTAA AGGTTCCTTG GTTCCCTAAG TCCAACTACT AAACTGGGGG ATATTATGAA GGGCCTTGAG CATCTGGATT CTGCCTAATA AAAAACATTT ATTTTCATTG CAATGATGTA TTTAAATTAT TTCTGAATAT TTTACTAAAA AGGGAATGTG GGAGGTCAGT GCATTTAAAA CATAAAGAAA TGAAGAGCTA GTTCAAACCT TGGGAAAATA CACTATATCT TAAACTCCAT GAAAGAAGGT GAGGCTGCAA ACAGCTAATG CACATTGGCA ACAGCCCTGA TGCCTATGCC TTATTCATCC CTCAGAAAAG GATTCAAGTA GAGGCTTGAT TTGGAGGTTA AAGTTTGGCT ATGCTGTATT TTACATTACT TATTGTTTTA GCTGTCCTCA TGAATGTCTT TTCACTACCC ATTTGCTTAT CCTGCATCTC TCAGCCTTGA CTCCACTCAG TTCTCTTGCT TAGAGATACC ACCTTTCCCC TGAAGTGTTC CTTCCATGTT TTACGGCGAG ATGGTTTCTC CTCGCCTGGC CACTCAGCCT TAGTTGTCTC TGTTGTCTTA TAGAGGTCTA CTTGAAGAAG GAAAAACAGG GGGCATGGTT TGACTGTCCT GTGAGCCCTT CTTCCCTGCC TCCCCCACTC ACAGTGACCC GGAATCCCTC GACATGGCAG TCTAGCACTA GTGCGGCCGC AGATCTGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGT
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