pDIRECT23C-CmYLCV-Csy4-BRI1-sgRNA1-sgRNA2
$113.00
In stock
SKU
PL52738
100μg liquid plasmid(Instantly):$134
| name | pDIRECT23C-CmYLCV-Csy4-BRI1-sgRNA1-sgRNA2 | another name | apDIRECT23C-CmYLCV-Csy4-BRI1-sgRNA1-sgRNA2 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Bar | Fluorescent labeling | |
| Promoter | Replicon | pVS1 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGC ATTCTAGGTA CTAAAACAAT TCATCCAGTA AAATATAATA TTTTATTTTC TCCCAATCAG GCTTGATCCC CAGTAAGTCA AAAAATAGCT CGACATACTG TTCTTCCCCG ATATCCTCCC TGATCGACCG GACGCAGAAG GCAATGTCAT ACCACTTGTC CGCCCTGCCG CTTCTCCCAA GATCAATAAA GCCACTTACT TTGCCATCTT TCACAAAGAT GTTGCTGTCT CCCAGGTCGC CGTGGGAAAA GACAAGTTCC TCTTCGGGCT TTTCCGTCTT TAAAAAATCA TACAGCTCGC GCGGATCTTT AAATGGAGTG TCTTCTTCCC AGTTTTCGCA ATCCACATCG GCCAGATCGT TATTCAGTAA GTAATCCAAT TCGGCTAAGC GGCTGTCTAA GCTATTCGTA TAGGGACAAT CCGATATGTC GATGGAGTGA AAGAGCCTGA TGCACTCCGC ATACAGCTCG ATAATCTTTT CAGGGCTTTG TTCATCTTCA TACTCTTCCG AGCAAAGGAC GCCATCGGCC TCACTCATGA GCAGATTGCT CCAGCCATCA TGCCGTTCAA AGTGCAGGAC CTTTGGAACA GGCAGCTTTC CTTCCAGCCA TAGCATCATG TCCTTTTCCC GTTCCACATC ATAGGTGGTC CCTTTATACC GGCTGTCCGT CATTTTTAAA TATAGGTTTT CATTTTCTCC CACCAGCTTA TATACCTTAG CAGGAGACAT TCCTTCCGTA TCTTTTACGC AGCGGTATTT TTCGATCAGT TTTTTCAATT CCGGTGATAT TCTCATTTTA GCCATTTATT ATTTCCTTCC TCTTTTCTAC AGTATTTAAA GATACCCCAA GAAGCTAATT ATAACAAGAC GAACTCCAAT TCACTGTTCC TTGCATTCTA AAACCTTAAA TACCAGAAAA CAGCTTTTTC AAAGTTGTTT TCAAAGTTGG CGTATAACAT AGTATCGACG GAGCCGATTT TGAAACCGCG GTGATCACAG GCAGCAACGC TCTGTCATCG TTACAATCAA CATGCTACCC TCCGCGAGAT CATCCGTGTT TCAAACCCGG CAGCTTAGTT GCCGTTCTTC CGAATAGCAT CGGTAACATG AGCAAAGTCT GCCGCCTTAC AACGGCTCTC CCGCTGACGC CGTCCCGGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGAAT TAACGCCGAA TTAATTCGGG GGATCTGGAT TTTAGTACTG GATTTTGGTT TTAGGAATTA GAAATTTTAT TGATAGAAGT ATTTTACAAA TACAAATACA TACTAAGGGT TTCTTATATG CTCAACACAT GAGCGAAACC CTATAGGAAC CCTAATTCCC TTATCTGGGA ACTACTCACA CATTATTATG GAGAAACTCG AGTCAAATCT CGGTGACGGG CAGGACCGGA CGGGGCGGTA CCGGCAGGCT GAAGTCCAGC TGCCAGAAAC CCACGTCATG CCAGTTCCCG TGCTTGAAGC CGGCCGCCCG CAGCATGCCG CGGGGGGCAT ATCCGAGCGC CTCGTGCATG CGCACGCTCG GGTCGTTGGG CAGCCCGATG ACAGCGACCA CGCTCTTGAA GCCCTGTGCC TCCAGGGACT TCAGCAGATG GGTGTAGAGC GTGGAGCCCA GTCCCGTCCG CTGGTGGCGG GGGCTGACGT ACACGGTCGA CTCGGCCGTC CAGTCGTAGG CGTTGCGTGC CTTCCAGGGG CCCGCGTAGG CGATGCCGGC GACCTCGCCG TCCACCTCGG CGACGAGCCA GGGATAGCGC TCCCGCAGAC GGACGAGGTC GTCCGTCCAC TCCTGCGGTT CCTGCGGCTC GGTACGGAAG TTGACCGTGC TTGTCTCGAT GTAGTGGTTG ACGATGGTGC AGACCGCCGG CATGTCCGCC TCGGTGGCAC GGCGGATGTC GGCCGGGCGT CGTTCTGGGC TCATGGTAGA CTCGAGAGAG ATAGATTTGT AGAGAGAGAC TGGTGATTTC AGCGTGTCCT CTCCAAATGA AATGAACTTC CTTATATAGA GGAAGGGTCT TGCGAAGGAT AGTGGGATTG TGCGTCATCC CTTACGTCAG TGGAGATATC ACATCAATCC ACTTGCTTTG AAGACGTGGT TGGAACGTCT TCTTTTTCCA CGATGCTCCT CGTGGGTGGG GGTCCATCTT TGGGACCACT GTCGGCAGAG GCATCTTGAA CGATAGCCTT TCCTTTATCG CAATGATGGC ATTTGTAGGT GCCACCTTCC TTTTCTACTG TCCTTTTGAT GAAGTGACAG ATAGCTGGGC AATGGAATCC GAGGAGGTTT CCCGATATTA CCCTTTGTTG AAAAGTCTCA ATAGCCCTTT GGTCTTCTGA GACTGTATCT TTGATATTCT TGGAGTAGAC GAGAGTGTCG TGCTCCACCA TGTTCACATC AATCCACTTG CTTTGAAGAC GTGGTTGGAA CGTCTTCTTT TTCCACGATG CTCCTCGTGG GTGGGGGTCC ATCTTTGGGA CCACTGTCGG CAGAGGCATC TTGAACGATA GCCTTTCCTT TATCGCAATG ATGGCATTTG TAGGTGCCAC CTTCCTTTTC TACTGTCCTT TTGATGAAGT GACAGATAGC TGGGCAATGG AATCCGAGGA GGTTTCCCGA TATTACCCTT TGTTGAAAAG TCTCAATAGC CCTTTGGTCT TCTGAGACTG TATCTTTGAT ATTCTTGGAG TAGACGAGAG TGTCGTGCTC CACCATGTTG GCAAGCTGCT CTAGCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC ATGATTACGA ATTCGAGCTC GGTACCCGGG GATCGGCGCG CCAGATTTGC CTTTTCAATT TCAGAAAGAA TGCTAACCCA CAGATGGTTA GAGAGGCTTA CGCAGCAGGT ATCATCAAGA CGATCTACCC GAGCAATAAT CTCCAGGAAA TCAAATACCT TCCCAAGAAG GTTAAAGATG CAGTCAAAAG ATTCAGGACT AACTGCATCA AGAACACAGA GAAAGATATA TTTCTCAAGA TCAGAAGTAC TATTCCAGTA TGGACGATTC AAGGCTTGCT TCACAAACCA AGGCAAGTAA TAGAGATTGG AGTCTCTAAA AAGGTAGTTC CCACTGAATC AAAGGCCATG GAGTCAAAGA TTCAAATAGA GGACCTAACA GAACTCGCCG TAAAGACTGG CGAACAGTTC ATACAGAGTC TCTTACGACT CAATGACAAG AAGAAAATCT TCGTCAACAT GGTGGAGCAC GACACACTTG TCTACTCCAA AAATATCAAA GATACAGTCT CAGAAGACCA AAGGGCAATT GAGACTTTTC AACAAAGGGT AATATCCGGA AACCTCCTCG GATTCCATTG CCCAGCTATC TGTCACTTTA TTGTGAAGAT AGTGGAAAAG GAAGGTGGCT CCTACAAATG CCATCATTGC GATAAAGGAA AGGCCATCGT TGAAGATGCC TCTGCCGACA GTGGTCCCAA AGATGGACCC CCACCCACGA GGAGCATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC AAAGCAAGTG GATTGATGTG ATATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA TCCTTCGCAA GACCCTTCCT CTATATAAGG AAGTTCATTT CATTTGGAGA GAACACGGGG GACTCCTGCA GGAAAAATGG ATCATTATCT TGATATTAGA CTTAGACCTG ATCCAGAATT TCCACCAGCT CAACTTATGT CTGTTCTTTT TGGAAAACTT CATCAAGCTC TTGTTGCTCA AGGAGGAGAT AGAATTGGAG TTTCTTTTCC TGATCTTGAT GAATCAAGAT CAAGACTTGG AGAAAGACTT AGAATTCATG CTTCTGCTGA TGATCTTAGA GCTTTGCTTG CTAGACCTTG GCTTGAAGGA CTTAGAGATC ATCTTCAATT TGGAGAACCA GCTGTTGTTC CACATCCAAC TCCTTATAGA CAAGTTTCAA GAGTTCAAGC TAAATCTAAT CCAGAAAGAC TTAGAAGAAG ACTTATGAGA AGACATGATC TTTCTGAAGA AGAAGCTAGA AAAAGAATTC CTGATACTGT TGCTAGAGCT TTGGATTTGC CTTTTGTTAC ACTTAGATCA CAATCTACTG GACAACATTT TAGACTTTTT ATTAGACATG GACCACTTCA AGTTACTGCT GAAGAAGGAG GATTTACTTG TTATGGACTT TCTAAGGGAG GTTTTGTTCC TTGGTTTGGA TCTGGAGCTA CTAATTTTTC TCTTCTTAAG CAAGCTGGAG ATGTTGAAGA AAATCCTGGA CCCATGGATA AGAAGTACTC TATCGGACTC GATATCGGAA CTAACTCTGT GGGATGGGCT GTGATCACCG ATGAGTACAA GGTGCCATCT AAGAAGTTCA AGGTTCTCGG AAACACCGAT AGGCACTCTA TCAAGAAAAA CCTTATCGGT GCTCTCCTCT TCGATTCTGG TGAAACTGCT GAGGCTACCA GACTCAAGAG AACCGCTAGA AGAAGGTACA CCAGAAGAAA GAACAGGATC TGCTACCTCC AAGAGATCTT CTCTAACGAG ATGGCTAAAG TGGATGATTC ATTCTTCCAC AGGCTCGAAG AGTCATTCCT CGTGGAAGAA GATAAGAAGC ACGAGAGGCA CCCTATCTTC GGAAACATCG TTGATGAGGT GGCATACCAC GAGAAGTACC CTACTATCTA CCACCTCAGA AAGAAGCTCG TTGATTCTAC TGATAAGGCT GATCTCAGGC TCATCTACCT CGCTCTCGCT CACATGATCA AGTTCAGAGG ACACTTCCTC ATCGAGGGTG ATCTCAACCC TGATAACTCT GATGTGGATA AGTTGTTCAT CCAGCTCGTG CAGACCTACA ACCAGCTTTT CGAAGAGAAC CCTATCAACG CTTCAGGTGT GGATGCTAAG GCTATCCTCT CTGCTAGGCT CTCTAAGTCA AGAAGGCTTG AGAACCTCAT TGCTCAGCTC CCTGGTGAGA AGAAGAACGG ACTTTTCGGA AACTTGATCG CTCTCTCTCT CGGACTCACC CCTAACTTCA AGTCTAACTT CGATCTCGCT GAGGATGCAA AGCTCCAGCT CTCAAAGGAT ACCTACGATG ATGATCTCGA TAACCTCCTC GCTCAGATCG GAGATCAGTA CGCTGATTTG TTCCTCGCTG CTAAGAACCT CTCTGATGCT ATCCTCCTCA GTGATATCCT CAGAGTGAAC ACCGAGATCA CCAAGGCTCC ACTCTCAGCT TCTATGATCA AGAGATACGA TGAGCACCAC CAGGATCTCA CACTTCTCAA GGCTCTTGTT AGACAGCAGC TCCCAGAGAA GTACAAAGAG ATTTTCTTCG ATCAGTCTAA GAACGGATAC GCTGGTTACA TCGATGGTGG TGCATCTCAA GAAGAGTTCT ACAAGTTCAT CAAGCCTATC CTCGAGAAGA TGGATGGAAC CGAGGAACTC CTCGTGAAGC TCAATAGAGA GGATCTTCTC AGAAAGCAGA GGACCTTCGA TAACGGATCT ATCCCTCATC AGATCCACCT CGGAGAGTTG CACGCTATCC TTAGAAGGCA AGAGGATTTC TACCCATTCC TCAAGGATAA CAGGGAAAAG ATTGAGAAGA TTCTCACCTT CAGAATCCCT TACTACGTGG GACCTCTCGC TAGAGGAAAC TCAAGATTCG CTTGGATGAC CAGAAAGTCT GAGGAAACCA TCACCCCTTG GAACTTCGAA GAGGTGGTGG ATAAGGGTGC TAGTGCTCAG TCTTTCATCG AGAGGATGAC CAACTTCGAT AAGAACCTTC CAAACGAGAA GGTGCTCCCT AAGCACTCTT TGCTCTACGA GTACTTCACC GTGTACAACG AGTTGACCAA GGTTAAGTAC GTGACCGAGG GAATGAGGAA GCCTGCTTTT TTGTCAGGTG AGCAAAAGAA GGCTATCGTT GATCTCTTGT TCAAGACCAA CAGAAAGGTG ACCGTGAAGC AGCTCAAAGA GGATTACTTC AAGAAAATCG AGTGCTTCGA TTCAGTTGAG ATTTCTGGTG TTGAGGATAG GTTCAACGCA TCTCTCGGAA CCTACCACGA TCTCCTCAAG ATCATTAAGG ATAAGGATTT CTTGGATAAC GAGGAAAACG AGGATATCTT GGAGGATATC GTTCTTACCC TCACCCTCTT TGAAGATAGA GAGATGATTG AAGAAAGGCT CAAGACCTAC GCTCATCTCT TCGATGATAA GGTGATGAAG CAGTTGAAGA GAAGAAGATA CACTGGTTGG GGAAGGCTCT CAAGAAAGCT CATTAACGGA ATCAGGGATA AGCAGTCTGG AAAGACAATC CTTGATTTCC TCAAGTCTGA TGGATTCGCT AACAGAAACT TCATGCAGCT CATCCACGAT GATTCTCTCA CCTTTAAAGA GGATATCCAG AAGGCTCAGG TTTCAGGACA GGGTGATAGT CTCCATGAGC ATATCGCTAA CCTCGCTGGA TCTCCTGCAA TCAAGAAGGG AATCCTCCAG ACTGTGAAGG TTGTGGATGA GTTGGTGAAG GTGATGGGAA GGCATAAGCC TGAGAACATC GTGATCGAAA TGGCTAGAGA GAACCAGACC ACTCAGAAGG GACAGAAGAA CTCTAGGGAA AGGATGAAGA GGATCGAGGA AGGTATCAAA GAGCTTGGAT CTCAGATCCT CAAAGAGCAC CCTGTTGAGA ACACTCAGCT CCAGAATGAG AAGCTCTACC TCTACTACCT CCAGAACGGA AGGGATATGT ATGTGGATCA AGAGTTGGAT ATCAACAGGC TCTCTGATTA CGATGTTGAT CATATCGTGC CACAGTCATT CTTGAAGGAT GATTCTATCG ATAACAAGGT GCTCACCAGG TCTGATAAGA ACAGGGGTAA GAGTGATAAC GTGCCAAGTG AAGAGGTTGT GAAGAAAATG AAGAACTATT GGAGGCAGCT CCTCAACGCT AAGCTCATCA CTCAGAGAAA GTTCGATAAC TTGACTAAGG CTGAGAGGGG AGGACTCTCT GAATTGGATA AGGCAGGATT CATCAAGAGG CAGCTTGTGG AAACCAGGCA GATCACTAAG CACGTTGCAC AGATCCTCGA TTCTAGGATG AACACCAAGT ACGATGAGAA CGATAAGTTG ATCAGGGAAG TGAAGGTTAT CACCCTCAAG TCAAAGCTCG TGTCTGATTT CAGAAAGGAT TTCCAATTCT ACAAGGTGAG GGAAATCAAC AACTACCACC ACGCTCACGA TGCTTACCTT AACGCTGTTG TTGGAACCGC TCTCATCAAG AAGTATCCTA AGCTCGAGTC AGAGTTCGTG TACGGTGATT ACAAGGTGTA CGATGTGAGG AAGATGATCG CTAAGTCTGA GCAAGAGATC GGAAAGGCTA CCGCTAAGTA TTTCTTCTAC TCTAACATCA TGAATTTCTT CAAGACCGAG ATTACCCTCG CTAACGGTGA GATCAGAAAG AGGCCACTCA TCGAGACAAA CGGTGAAACA GGTGAGATCG TGTGGGATAA GGGAAGGGAT TTCGCTACCG TTAGAAAGGT GCTCTCTATG CCACAGGTGA ACATCGTTAA GAAAACCGAG GTGCAGACCG GTGGATTCTC TAAAGAGTCT ATCCTCCCTA AGAGGAACTC TGATAAGCTC ATTGCTAGGA AGAAGGATTG GGACCCTAAG AAATACGGTG GTTTCGATTC TCCTACCGTG GCTTACTCTG TTCTCGTTGT GGCTAAGGTT GAGAAGGGAA AGAGTAAGAA GCTCAAGTCT GTTAAGGAAC TTCTCGGAAT CACTATCATG GAAAGGTCAT CTTTCGAGAA GAACCCAATC GATTTCCTCG AGGCTAAGGG ATACAAAGAG GTTAAGAAGG ATCTCATCAT CAAGCTCCCA AAGTACTCAC TCTTCGAACT CGAGAACGGT AGAAAGAGGA TGCTCGCTTC TGCTGGTGAG CTTCAAAAGG GAAACGAGCT TGCTCTCCCA TCTAAGTACG TTAACTTTCT TTACCTCGCT TCTCACTACG AGAAGTTGAA GGGATCTCCA GAAGATAACG AGCAGAAGCA ACTTTTCGTT GAGCAGCACA AGCACTACTT GGATGAGATC ATCGAGCAGA TCTCTGAGTT CTCTAAAAGG GTGATCCTCG CTGATGCAAA CCTCGATAAG GTGTTGTCTG CTTACAACAA GCACAGAGAT AAGCCTATCA GGGAACAGGC AGAGAACATC ATCCATCTCT TCACCCTTAC CAACCTCGGT GCTCCTGCTG CTTTCAAGTA CTTCGATACA ACCATCGATA GGAAGAGATA CACCTCTACC AAAGAAGTGC TCGATGCTAC CCTCATCCAT CAGTCTATCA CTGGACTCTA CGAGACTAGG ATCGATCTCT CACAGCTCGG TGGTGATTCA AGGGCTGATC CTAAGAAGAA GAGGAAGGTT TGACTCGAGA TATGAAGATG AAGATGAAAT ATTTGGTGTG TCAAATAAAA AGCTTGTGTG CTTAAGTTTG TGTTTTTTTC TTGGCTTGTT GTGTTATGAA TTTGTGGCTT TTTCTAATAT TAAATGAATG TAAGATCACA TTATAATGAA TAAACAAATG TTTCTATAAT CCATTGTGAA TGTTTTGTTG GATCTCTTCT GCAGCATATA ACTACTGTAT GTGCTATGGT ATGGACTATG GAATATGATT AAAGATAAGG AGCTCCGGTG ACGGACGGCG CGCTGGCAGA CATACTGTCC CACAAATGAA GATGGAATCT GTAAAAGAAA ACGCGTGAAA TAATGCGTCT GACAAAGGTT AGGTCGGCTG CCTTTAATCA ATACCAAAGT GGTCCCTACC ACGATGGAAA AACTGTGCAG TCGGTTTGGC TTTTTCTGAC GAACAAATAA GATTCGTGGC CGACAGGTGG GGGTCCACCA TGTGAAGGCA TCTTCAGACT CCAATAATGG AGCAATGACG TAAGGGCTTA CGAAATAAGT AAGGGTAGTT TGGGAAATGT CCACTCACCC GTCAGTCTAT AAATACTTAG CCCCTCCCTC ATTGTTAAGG GAGCAAAATC TCAGAGAGAT AGTCCTAGAG AGAGAAAGAG AGCAAGTAGC CTAGAAGTAG TCAAGGCGGC GAAGTATTCA GGCACGTGGC CAGGAAGAAG AAAAGCCAAG ACGACGAAAA CAGGTAAGAG CTAAGCTTCC TGCAGGTTCA CTGCCGTATA GGCAGTTGGG TCATAACGAT ATCTCGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CGTTCACTGC CGTATAGGCA GTAACGCCAT CGAAAGTACA CGTTTTAGAG CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCGTT CACTGCCGTA TAGGCAGGTC GATCGACAAG CTCGAGTTTC TCCATAATAA TGTGTGAGTA GTTCCCAGAT AAGGGAATTA GGGTTCCTAT AGGGTTTCGC TCATGTGTTG AGCATATAAG AAACCCTTAG TATGTATTTG TATTTGTAAA ATACTTCTAT CAATAAAATT TCTAATTCCT AAAACCAAAA TCCAGTACTA AAATCCAGAT CCCCCGAATT ACCGGTGGCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGCTAGA GCAGCTTGAG CTTGGATCAG ATTGTCGTTT CCCGCCTTCA GTTTAAACTA TCAGTGTTTG ACAGGATATA TTGGCGGGTA AACCTAAGAG AAAAGAGCGT TTATTAGAAT AATCGGATAT TTAAAAGGGC GTGAAAAGGT TTATCCGTTC GTCCATTTGT ATGTGCATGC CAACCACAGG GTTCCCCTCG GGATCAAAGT ACTTTGATCC AACCCCTCCG CTGCTATAGT GCAGTCGGCT TCTGACGTTC AGTGCAGCCG TCTTCTGAAA ACGACATGTC GCACAAGTCC TAAGTTACGC GACAGGCTGC CGCCCTGCCC TTTTCCTGGC GTTTTCTTGT CGCGTGTTTT AGTCGCATAA AGTAGAATAC TTGCGACTAG AACCGGAGAC ATTACGCCAT GAACAAGAGC GCCGCCGCTG GCCTGCTGGG CTATGCCCGC GTCAGCACCG ACGACCAGGA CTTGACCAAC CAACGGGCCG AACTGCACGC GGCCGGCTGC ACCAAGCTGT TTTCCGAGAA GATCACCGGC ACCAGGCGCG ACCGCCCGGA GCTGGCCAGG ATGCTTGACC ACCTACGCCC TGGCGACGTT GTGACAGTGA CCAGGCTAGA CCGCCTGGCC CGCAGCACCC GCGACCTACT GGACATTGCC GAGCGCATCC AGGAGGCCGG CGCGGGCCTG CGTAGCCTGG CAGAGCCGTG GGCCGACACC ACCACGCCGG CCGGCCGCAT GGTGTTGACC GTGTTCGCCG GCATTGCCGA GTTCGAGCGT TCCCTAATCA TCGACCGCAC CCGGAGCGGG CGCGAGGCCG CCAAGGCCCG AGGCGTGAAG TTTGGCCCCC GCCCTACCCT CACCCCGGCA CAGATCGCGC ACGCCCGCGA GCTGATCGAC CAGGAAGGCC GCACCGTGAA AGAGGCGGCT GCACTGCTTG GCGTGCATCG CTCGACCCTG TACCGCGCAC TTGAGCGCAG CGAGGAAGTG ACGCCCACCG AGGCCAGGCG GCGCGGTGCC TTCCGTGAGG ACGCATTGAC CGAGGCCGAC GCCCTGGCGG CCGCCGAGAA TGAACGCCAA GAGGAACAAG CATGAAACCG CACCAGGACG GCCAGGACGA ACCGTTTTTC ATTACCGAAG AGATCGAGGC GGAGATGATC GCGGCCGGGT ACGTGTTCGA GCCGCCCGCG CACGGCTCAA CCGTGCGGCT GCATGAAATC CTGGCCGGTT TGTCTGATGC CAAGCTGGCG GCCTGGCCGG CCAGCTTGGC CGCTGAAGAA ACCGAGCGCC GCCGTCTAAA AAGGTGATGT GTATTTGAGT AAAACAGCTT GCGTCATGCG GTCGCTGCGT ATATGATGCG ATGAGTAAAT AAACAAATAC GCAAGGGGAA CGCATGAAGG TTATCGCTGT ACTTAACCAG AAAGGCGGGT CAGGCAAGAC GACCATCGCA ACCCATCTAG CCCGCGCCCT GCAACTCGCC GGGGCCGATG TTCTGTTAGT CGATTCCGAT CCCCAGGGCA GTGCCCGCGA TTGGGCGGCC GTGCGGGAAG ATCAACCGCT AACCGTTGTC GGCATCGACC GCCCGACGAT TGACCGCGAC GTGAAGGCCA TCGGCCGGCG CGACTTCGTA GTGATCGACG GAGCGCCCCA GGCGGCGGAC TTGGCTGTGT CCGCGATCAA GGCAGCCGAC TTCGTGCTGA TTCCGGTGCA GCCAAGCCCT TACGACATAT GGGCCACCGC CGACCTGGTG GAGCTGGTTA AGCAGCGCAT TGAGGTCACG GATGGAAGGC TACAAGCGGC CTTTGTCGTG TCGCGGGCGA TCAAAGGCAC GCGCATCGGC GGTGAGGTTG CCGAGGCGCT GGCCGGGTAC GAGCTGCCCA TTCTTGAGTC CCGTATCACG CAGCGCGTGA GCTACCCAGG CACTGCCGCC GCCGGCACAA CCGTTCTTGA ATCAGAACCC GAGGGCGACG CTGCCCGCGA GGTCCAGGCG CTGGCCGCTG AAATTAAATC AAAACTCATT TGAGTTAATG AGGTAAAGAG AAAATGAGCA AAAGCACAAA CACGCTAAGT GCCGGCCGTC CGAGCGCACG CAGCAGCAAG GCTGCAACGT TGGCCAGCCT GGCAGACACG CCAGCCATGA AGCGGGTCAA CTTTCAGTTG CCGGCGGAGG ATCACACCAA GCTGAAGATG TACGCGGTAC GCCAAGGCAA GACCATTACC GAGCTGCTAT CTGAATACAT CGCGCAGCTA CCAGAGTAAA TGAGCAAATG AATAAATGAG TAGATGAATT TTAGCGGCTA AAGGAGGCGG CATGGAAAAT CAAGAACAAC CAGGCACCGA CGCCGTGGAA TGCCCCATGT GTGGAGGAAC GGGCGGTTGG CCAGGCGTAA GCGGCTGGGT TGCCTGCCGG CCCTGCAATG GCACTGGAAC CCCCAAGCCC GAGGAATCGG CGTGAGCGGT CGCAAACCAT CCGGCCCGGT ACAAATCGGC GCGGCGCTGG GTGATGACCT GGTGGAGAAG TTGAAGGCCG CGCAGGCCGC CCAGCGGCAA CGCATCGAGG CAGAAGCACG CCCCGGTGAA TCGTGGCAAG CGGCCGCTGA TCGAATCCGC AAAGAATCCC GGCAACCGCC GGCAGCCGGT GCGCCGTCGA TTAGGAAGCC GCCCAAGGGC GACGAGCAAC CAGATTTTTT CGTTCCGATG CTCTATGACG TGGGCACCCG CGATAGTCGC AGCATCATGG ACGTGGCCGT TTTCCGTCTG TCGAAGCGTG ACCGACGAGC TGGCGAGGTG ATCCGCTACG AGCTTCCAGA CGGGCACGTA GAGGTTTCCG CAGGGCCGGC CGGCATGGCC AGTGTGTGGG ATTACGACCT GGTACTGATG GCGGTTTCCC ATCTAACCGA ATCCATGAAC CGATACCGGG AAGGGAAGGG AGACAAGCCC GGCCGCGTGT TCCGTCCACA CGTTGCGGAC GTACTCAAGT TCTGCCGGCG AGCCGATGGC GGAAAGCAGA AAGACGACCT GGTAGAAACC TGCATTCGGT TAAACACCAC GCACGTTGCC ATGCAGCGTA CGAAGAAGGC CAAGAACGGC CGCCTGGTGA CGGTATCCGA GGGTGAAGCC TTGATTAGCC GCTACAAGAT CGTAAAGAGC GAAACCGGGC GGCCGGAGTA CATCGAGATC GAGCTAGCTG ATTGGATGTA CCGCGAGATC ACAGAAGGCA AGAACCCGGA CGTGCTGACG GTTCACCCCG ATTACTTTTT GATCGATCCC GGCATCGGCC GTTTTCTCTA CCGCCTGGCA CGCCGCGCCG CAGGCAAGGC AGAAGCCAGA TGGTTGTTCA AGACGATCTA CGAACGCAGT GGCAGCGCCG GAGAGTTCAA GAAGTTCTGT TTCACCGTGC GCAAGCTGAT CGGGTCAAAT GACCTGCCGG AGTACGATTT GAAGGAGGAG GCGGGGCAGG CTGGCCCGAT CCTAGTCATG CGCTACCGCA ACCTGATCGA GGGCGAAGCA TCCGCCGGTT CCTAATGTAC GGAGCAGATG CTAGGGCAAA TTGCCCTAGC AGGGGAAAAA GGTCGAAAAG GCCTCTTTCC TGTGGATAGC ACGTACATTG GGAACCCAAA GCCGTACATT GGGAACCGGA ACCCGTACAT TGGGAACCCA AAGCCGTACA TTGGGAACCG GTCACACATG TAAGTGACTG ATATAAAAGA GAAAAAAGGC GATTTTTCCG CCTAAAACTC TTTAAAACTT ATTAAAACTC TTAAAACCCG CCTGGCCTGT GCATAACTGT CTGGCCAGCG CACAGCCCAA GAGCTGCAAA AAGCGCCTAC CCTTCGGTCG CTGCGCTCCC TACGCCCCGC CGCTTCGCGT CGGCCTATCG CGGCCGCTGG CCGCTCAAAA ATGGCTGGCC TACGGCCAGG CAATCTACCA GGGCGCGGAC AAGCCGCGCC GTCGCCACTC GACCGCCGGC GCCCACATCA AGGCACCCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAAACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC CCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTAT
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