pCMV-EGFP-REST(mouse)-SV40-Neo
$99.00
In stock
SKU
PL52675
NM_011263.2;2610008J04Rik; AA407358; NRSF; REST4
100μg liquid plasmid(Instantly):$120
| name | pCMV-EGFP-REST(mouse)-SV40-Neo | another name | apCMV-EGFP-REST(mouse)-SV40-Neo |
| Insert Size (bp) | 3249bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCTCGAGGC GGCGGAGGGA GTGGAGGAGG GGGAAGCAAG CTTCGAATTC TGATGGCCAC CCAGGTGATG GGGCAGTCTT CTGGAGGAGG CAGTCTCTTC AACAACAGTG CCAACATGGG CATGGCCTTA ACCAACGACA TGTACGACCT GCACGAGCTC TCGAAAGCTG AACTGGCAGC CCCTCAGCTC ATCATGTTAG CCAACGTGGC CCTGACGGGG GAGGCAAGCG GCAGCTGCTG CGATTACCTG GTCGGTGAAG AGAGGCAGAT GGCCGAATTG ATGCCCGTGG GAGACAACCA CTTCTCAGAA AGTGAAGGAG AAGGCCTGGA AGAGTCGGCT GACCTCAAAG GGCTGGAAAA CATGGAACTG GGAAGTTTGG AGCTAAGTGC TGTAGAACCC CAGCCCGTAT TTGAAGCCTC AGCTGCCCCA GAAATATACA GCGCCAATAA AGATCCCGCT CCAGAAACAC CCGTGGCGGA AGACAAATGC AGGAGTTCTA AGGCCAAGCC CTTCCGGTGT AAGCCTTGCC AGTACGAAGC CGAATCTGAA GAGCAGTTTG TGCATCACAT CCGGATTCAC AGCGCTAAGA AGTTCTTTGT GGAGGAAAGT GCAGAGAAAC AGGCCAAAGC CTGGGAGTCG GGGTCGTCTC CGGCCGAAGA GGGCGAGTTC TCCAAAGGCC CCATCCGCTG TGACCGCTGT GGCTACAATA CCAACCGGTA TGACCACTAC ATGGCACACC TGAAGCACCA CCTGCGAGCT GGCGAGAACG AGCGCATCTA CAAGTGCATC ATCTGCACGT ACACGACGGT CAGCGAGTAC CACTGGAGGA AACACCTGAG AAACCATTTC CCCAGGAAAG TCTACACCTG CAGCAAGTGC AACTACTTCT CAGACAGAAA AAATAACTAC GTTCAGCACG TGCGAACTCA CACAGGAGAA CGCCCGTATA AATGTGAACT TTGTCCTTAC TCAAGCTCTC AGAAGACTCA TCTAACGCGA CACATGCGGA CTCATTCAGG TGAGAAGCCA TTTAAATGTG ATCAGTGCAA TTATGTGGCC TCTAATCAGC ATGAAGTGAC CCGACATGCA AGACAGGTTC ACAACGGGCC TAAACCTCTT AATTGCCCGC ACTGTGACTA CAAAACAGCA GATAGAAGCA ACTTCAAAAA GCACGTGGAG CTGCATGTTA ACCCACGGCA GTTCAACTGC CCCGTGTGTG ACTACGCGGC TTCTAAGAAG TGTAATCTAC AATACCATTT CAAATCTAAG CATCCCACCT GTCCCAGCAA AACAATGGAT GTCTCCAAAG TGAAGCTAAA GAAAACCAAA AAGAGAGAGG CTGACCTGCT TAATAACGCC GTCAGCAACG AGAAGATGGA GAATGAGCAA ACAAAAACAA AGGGGGATGT GTCTGGGAAG AAGAACGAGA AACCTGTAAA AGCTGTGGGA AAAGATGCTT CAAAAGAGAA GAAGCCTGGT AGCAGTGTCT CAGTGGTCCA GGTAACTACC AGGACTCGGA AGTCAGCGGT GGCGGCGGAG ACTAAAGCAG CAGAGGTGAA ACACACAGAC GGACAAACAG GAAACAATCC AGAAAAGCCC TGTAAAGCCA AGAAAAACAA AAGAAAGAAG GATGCTGAGG CCCATCCCTC CGAAGAGCCT GTGAACGAGG GACCAGTGAC AAAAAAGAAA AAGAAGTCTG AGTGCAAATC AAAAATCGGT ACCAACGTGC CAAAGGGCGG CGGCCGAGCG GAGGAGAGGC CGGGGGTCAA GAAGCAAAGC GCTTCCCTTA AGAAAGGCAC AAAGAAGACG CCGCCCAAGA CAAAGACAAG TAAAAAAGGT GGCAAACTTG CTCCAAAGGG GATGGGGCAG ACAGAACCTT CTTCTGGGGC ATTGGCTCAA GTGGGGGTGT CTCCAGACCC TGCCCTCATT CAGGCAGAGG TCACCGGGTC AGGATCTTCT CAGACAGAGC TTCCTTCACC CATGGATATT GCTAAGTCAG AGCCCGCCCA GATGGAGGTT TCCCTAACAG GGCCACCTCC GGTGGAGCCT GCTCAAATGG AGCCATCGCC TGCGAAACCT CCCCAGGTAG AAGCACCCAC TTACCCCCAG CCTCCCCAAA GGGGGCCTGC CCCTCCCACG GGGCCTGCCC CTCCCACGGG GCCTGCCCCT CCCACGGAGC CTGCCCCTCC CACGGGGCTT GCCGAGATGG AACCTTCTCC CACGGAGCCT TCCCAGAAGG AACCACCTCC CAGTATGGAG CCTCCCTGCC CCGAGGAGCT GCCTCAGGCC GAGCCACCTC CTATGGAGGA TTGTCAGAAG GAGCTGCCTT CTCCCGTGGA GCCCGCTCAG ATTGAGGTTG CTCAGACGGC CCCTACGCAG GTTCAGGAGG AGCCCCCTCC TGTCTCGGAG CCACCTCGGG TGAAGCCAAC CAAAAGATCA TCTCTCCGGA AAGACAGAGC AGAGAAGGAG CTGAGCCTGC TGAGTGAGAT GGCGCGGCAG GAGCAGGTCC TCATGGGGGT TGGCTTGGTG CCTGTTAGAG ACAGCAAGCT TCTGAAGGGA AACAAGAGCG CCCAGGACCC CCCAGCCCCA CCGTCACCAT CGCCAAAGGG AAACTCGAGG GAAGAGACAC CCAAGGACCA AGAAATGGTC TCTGATGGGG AAGGAACTAT AGTATTCCCT CTCAAGAAAG GAGGACCAGA GGAAGCTGGA GAGAGTCCAG CTGAGTTGGC TGCTCTCAAG GAGTCTGCCC GTGTTTCATC CTCTGAACAA AACTCAGCCA TGCCAGAGGG TGGAGCATCA CACAGCAAGT GTCAGACTGG CTCCTCTGGG CTTTGTGACG TGGACACTGA GCAGAAGACA GATACTGTCC CCATGAAAGA CTCCGCAGCA GAGCCAGTGT CCCCTCCTAC CCCAACAGTG GACCGTGACG CAGGGTCACC AGCTGTAGTG GCCTCCCCTC CTATCACGTT GGCTGAAAAC GAGTCTCAGG AAATTGATGA AGATGAAGGC ATCCATAGCC ATGATGGAAG TGACCTGAGT GACAACATGT CTGAGGGGAG TGACGACTCA GGACTGCACG GGGCTCGGCC GACACCACCA GAAGCTACGT CAAAAAATGG GAAGGCAGGG TTGGCTGGTA AAGTGACTGA GGGAGAGTTT GTGTGTATTT TCTGTGATCG TTCTTTTAGA AAGGAAAAAG ATTATAGCAA ACACCTCAAT CGCCACTTGG TGAATGTGTA CTTCCTAGAA GAAGCAGCTG AGGAGCAGGA GGAGCAGGAG GAGCGGGAGG AGCAGGAGTA GGGATCCACC GGATCTAGAT AACTGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TAACGCGTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTCCTGA GGCGGAAAGA ACCAGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCTAGGG GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG AACCCGCGCT ATGACGGCAA TAAAAAGACA GAATAAAACG CACGGTGTTG GGTCGTTTGT TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCCT CAGGTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCATG CAT
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