pNRF2(mouse)-mut-Fluc-hPEST-SV40-Neo
$70.00
In stock
SKU
PL52517
100μg liquid plasmid(Instantly):$91
| name | pNRF2(mouse)-mut-Fluc-hPEST-SV40-Neo | another name | apNRF2(mouse)-mut-Fluc-hPEST-SV40-Neo |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Rluc,Fluc | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CAGGTGCCAG AACATTTCTC TGGCCTAACT GGCCGGTACC ATTAGTTATG AAGTAGCAGC AAAAATACTT TTCTAGTTGA TCGGTGTCAC AACACGAACT ATATTAAAGG GTCGCTGCAT TACGACGGTT GAGAAACGCT GCTCTAAAAA CAGCTTGAGA GGTGGGGCCT TATGAGATCA TACCTCAAAA TTCCCAAATA GAGTAAGTAC GTGTAAAGGA ACCCTGAGAG TCTTAGAATC CACTTCGGTG ATTCTGTTAG AGCTGTGCCC GCTTGCTGGG CTCTGGGTGG ATGAAGTGAT ACTGAATCGG TGTTTGGGCC CCGAGCCCAG GGCACGTGGG AGAAGTGGAG GGCCTTCGAG TAAGCGAGCA CGAGTTTGCA GCGTGGACTC ATCCATCTCC CTGGGGCAGA GGTGTTTGAG CACCAGCAAC TGAATGGGAC CTGGGGCCAG AGGGCAGATC GGTGTTTGCA AAGTCCGGGT CCCAGCTCAG AGCCCTAACC CCGAAACTGG CGCCACAGTC AGCCGGTGGA GCGAGATAAA GCGGGCAAGG TTCTGCAGGT CCAAATCAGG GAGTGGCAGC TCCGATAGCA GCGCACGGGC CGGGGCTCCC GATCGGTGTG CACCAACTGT TTAAACTGCT TCAAAGAGCC AGGGCTGGAA TCGGTGTCGG CAAACAGCTG CTAATCTCTA AAGCTTGGCA ATCCGGTACT GTTGGTAAAG CCACCATGGA AGATGCCAAA AACATTAAGA AGGGCCCAGC GCCATTCTAC CCACTCGAAG ACGGGACCGC CGGCGAGCAG CTGCACAAAG CCATGAAGCG CTACGCCCTG GTGCCCGGCA CCATCGCCTT TACCGACGCA CATATCGAGG TGGACATTAC CTACGCCGAG TACTTCGAGA TGAGCGTTCG GCTGGCAGAA GCTATGAAGC GCTATGGGCT GAATACAAAC CATCGGATCG TGGTGTGCAG CGAGAATAGC TTGCAGTTCT TCATGCCCGT GTTGGGTGCC CTGTTCATCG GTGTGGCTGT GGCCCCAGCT AACGACATCT ACAACGAGCG CGAGCTGCTG AACAGCATGG GCATCAGCCA GCCCACCGTC GTATTCGTGA GCAAGAAAGG GCTGCAAAAG ATCCTCAACG TGCAAAAGAA GCTACCGATC ATACAAAAGA TCATCATCAT GGATAGCAAG ACCGACTACC AGGGCTTCCA AAGCATGTAC ACCTTCGTGA CTTCCCATTT GCCACCCGGC TTCAACGAGT ACGACTTCGT GCCCGAGAGC TTCGACCGGG ACAAAACCAT CGCCCTGATC ATGAACAGTA GTGGCAGTAC CGGATTGCCC AAGGGCGTAG CCCTACCGCA CCGCACCGCT TGTGTCCGAT TCAGTCATGC CCGCGACCCC ATCTTCGGCA ACCAGATCAT CCCCGACACC GCTATCCTCA GCGTGGTGCC ATTTCACCAC GGCTTCGGCA TGTTCACCAC GCTGGGCTAC TTGATCTGCG GCTTTCGGGT CGTGCTCATG TACCGCTTCG AGGAGGAGCT ATTCTTGCGC AGCTTGCAAG ACTATAAGAT TCAATCTGCC CTGCTGGTGC CCACACTATT TAGCTTCTTC GCTAAGAGCA CTCTCATCGA CAAGTACGAC CTAAGCAACT TGCACGAGAT CGCCAGCGGC GGGGCGCCGC TCAGCAAGGA GGTAGGTGAG GCCGTGGCCA AACGCTTCCA CCTACCAGGC ATCCGCCAGG GCTACGGCCT GACAGAAACA ACCAGCGCCA TTCTGATCAC CCCCGAAGGG GACGACAAGC CTGGCGCAGT AGGCAAGGTG GTGCCCTTCT TCGAGGCTAA GGTGGTGGAC TTGGACACCG GTAAGACACT GGGTGTGAAC CAGCGCGGCG AGCTGTGCGT CCGTGGCCCC ATGATCATGA GCGGCTACGT TAACAACCCC GAGGCTACAA ACGCTCTCAT CGACAAGGAC GGCTGGCTGC ACAGCGGCGA CATCGCCTAC TGGGACGAGG ACGAGCACTT CTTCATCGTG GACCGGCTGA AGAGCCTGAT CAAATACAAG GGCTACCAGG TAGCCCCAGC CGAACTGGAG AGCATCCTGC TGCAACACCC CAACATCTTC GACGCCGGGG TCGCCGGCCT GCCCGACGAC GATGCCGGCG AGCTGCCCGC CGCAGTCGTC GTGCTGGAAC ACGGTAAAAC CATGACCGAG AAGGAGATCG TGGACTATGT GGCCAGCCAG GTTACAACCG CCAAGAAGCT GCGCGGTGGT GTTGTGTTCG TGGACGAGGT GCCTAAAGGA CTGACCGGCA AGTTGGACGC CCGCAAGATC CGCGAGATTC TCATTAAGGC CAAGAAGGGC GGCAAGATCG CCGTGAATTC TCACGGCTTC CCTCCCGAGG TGGAGGAGCA GGCCGCCGGC ACCCTGCCCA TGAGCTGCGC CCAGGAGAGC GGCATGGATA GACACCCTGC TGCTTGCGCC AGCGCCAGGA TCAACGTCTA AGGCCGCGAC TCTAGAGTCG GGGCGGCCGG CCGCTTCGAG CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TAAAATCGAT AAGGATCCGT TTGCGTATTG GGCGCTCTTC CGCTGATCTG CGCAGCACCA TGGCCTGAAA TAACCTCTGA AAGAGGAACT TGGTTAGCTA CCTTCTGAGG CGGAAAGAAC CAGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCGA TTCTTCTGAC ACTAGCGCCA CCATGATCGA ACAAGACGGC CTCCATGCTG GCAGTCCCGC AGCTTGGGTC GAACGCTTGT TCGGGTACGA CTGGGCCCAG CAGACCATCG GATGTAGCGA TGCGGCCGTG TTCCGTCTAA GCGCTCAAGG CCGGCCCGTG CTGTTCGTGA AGACCGACCT GAGCGGCGCC CTGAACGAGC TTCAAGACGA GGCTGCCCGC CTGAGCTGGC TGGCCACCAC CGGCGTACCC TGCGCCGCTG TGTTGGATGT TGTGACCGAA GCCGGCCGGG ACTGGCTGCT GCTGGGCGAG GTCCCTGGCC AGGATCTGCT GAGCAGCCAC CTTGCCCCCG CTGAGAAGGT TTCTATCATG GCCGATGCAA TGCGGCGCCT GCACACCCTG GACCCCGCTA CCTGCCCCTT CGACCACCAG GCTAAGCATC GGATCGAGCG TGCTCGGACC CGCATGGAGG CCGGCCTGGT GGACCAGGAC GACCTGGACG AGGAGCATCA GGGCCTGGCC CCCGCTGAAC TGTTCGCCCG ACTGAAAGCC CGCATGCCGG ACGGTGAGGA CCTGGTTGTC ACACACGGAG ATGCCTGCCT CCCTAACATC ATGGTCGAGA ATGGCCGCTT CTCCGGCTTC ATCGACTGCG GTCGCCTAGG AGTTGCCGAC CGCTACCAGG ACATCGCCCT GGCCACCCGC GACATCGCTG AGGAGCTTGG CGGCGAGTGG GCCGACCGCT TCTTAGTCTT GTACGGCATC GCAGCTCCCG ACAGCCAGCG CATCGCCTTC TACCGCTTGC TCGACGAGTT CTTTTAATGA TCTAGAACCG GTCATGGCCG CAATAAAATA TCTTTATTTT CATTACATCT GTGTGTTGGT TTTTTGTGTG TTCGAACTAG ATGCTGTCGA CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC GGTGGGCGCG GGGCATGACT ATCGTCGCCG CACTTATGAC TGTCTTCTTT ATCATGCAAC TCGTAGGACA GGTGCCGGCA GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGCGGCC GCAAATGCTA AACCACTGCA GTGGTTACCA GTGCTTGATC AGTGAGGCAC CGATCTCAGC GATCTGCCTA TTTCGTTCGT CCATAGTGGC CTGACTCCCC GTCGTGTAGA TCACTACGAT TCGTGAGGGC TTACCATCAG GCCCCAGCGC AGCAATGATG CCGCGAGAGC CGCGTTCACC GGCCCCCGAT TTGTCAGCAA TGAACCAGCC AGCAGGGAGG GCCGAGCGAA GAAGTGGTCC TGCTACTTTG TCCGCCTCCA TCCAGTCTAT GAGCTGCTGT CGTGATGCTA GAGTAAGAAG TTCGCCAGTG AGTAGTTTCC GAAGAGTTGT GGCCATTGCT ACTGGCATCG TGGTATCACG CTCGTCGTTC GGTATGGCTT CGTTCAACTC TGGTTCCCAG CGGTCAAGCC GGGTCACATG ATCACCCATA TTATGAAGAA ATGCAGTCAG CTCCTTAGGG CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCGGTGTTGT CGCTCATGGT AATGGCAGCA CTACACAATT CTCTTACCGT CATGCCATCC GTAAGATGCT TTTCCGTGAC CGGCGAGTAC TCAACCAAGT CGTTTTGTGA GTAGTGTATA CGGCGACCAA GCTGCTCTTG CCCGGCGTCT ATACGGGACA ACACCGCGCC ACATAGCAGT ACTTTGAAAG TGCTCATCAT CGGGAATCGT TCTTCGGGGC GGAAAGACTC AAGGATCTTG CCGCTATTGA GATCCAGTTC GATATAGCCC ACTCTTGCAC CCAGTTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC GGGGTGTGCA AAAACAGGCA AGCAAAATGC CGCAAAGAAG GGAATGAGTG CGACACGAAA ATGTTGGATG CTCATACTCG TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTACTA GTACGTCTCT CAAGGATAAG TAAGTAATAT TAAGGTACGG GAGGTATTGG ACAGGCCGCA ATAAAATATC TTTATTTTCA TTACATCTGT GTGTTGGTTT TTTGTGTGAA TCGATAGTAC TAACATACGC TCTCCATCAA AACAAAACGA AACAAAACAA ACTAGCAAAA TAGGCTGTCC CCAGTGCAAG TG
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
