pDIRECT23A-AtU6-BRI1-sgRNA1-sgRNA2-35S-Cas9
$113.00
In stock
SKU
PL52494
100μg liquid plasmid(Instantly):$134
| name | pDIRECT23A-AtU6-BRI1-sgRNA1-sgRNA2-35S-Cas9 | another name | apDIRECT23A-AtU6-BRI1-sgRNA1-sgRNA2-35S-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Bar | Fluorescent labeling | |
| Promoter | Replicon | pVS1 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGC ATTCTAGGTA CTAAAACAAT TCATCCAGTA AAATATAATA TTTTATTTTC TCCCAATCAG GCTTGATCCC CAGTAAGTCA AAAAATAGCT CGACATACTG TTCTTCCCCG ATATCCTCCC TGATCGACCG GACGCAGAAG GCAATGTCAT ACCACTTGTC CGCCCTGCCG CTTCTCCCAA GATCAATAAA GCCACTTACT TTGCCATCTT TCACAAAGAT GTTGCTGTCT CCCAGGTCGC CGTGGGAAAA GACAAGTTCC TCTTCGGGCT TTTCCGTCTT TAAAAAATCA TACAGCTCGC GCGGATCTTT AAATGGAGTG TCTTCTTCCC AGTTTTCGCA ATCCACATCG GCCAGATCGT TATTCAGTAA GTAATCCAAT TCGGCTAAGC GGCTGTCTAA GCTATTCGTA TAGGGACAAT CCGATATGTC GATGGAGTGA AAGAGCCTGA TGCACTCCGC ATACAGCTCG ATAATCTTTT CAGGGCTTTG TTCATCTTCA TACTCTTCCG AGCAAAGGAC GCCATCGGCC TCACTCATGA GCAGATTGCT CCAGCCATCA TGCCGTTCAA AGTGCAGGAC CTTTGGAACA GGCAGCTTTC CTTCCAGCCA TAGCATCATG TCCTTTTCCC GTTCCACATC ATAGGTGGTC CCTTTATACC GGCTGTCCGT CATTTTTAAA TATAGGTTTT CATTTTCTCC CACCAGCTTA TATACCTTAG CAGGAGACAT TCCTTCCGTA TCTTTTACGC AGCGGTATTT TTCGATCAGT TTTTTCAATT CCGGTGATAT TCTCATTTTA GCCATTTATT ATTTCCTTCC TCTTTTCTAC AGTATTTAAA GATACCCCAA GAAGCTAATT ATAACAAGAC GAACTCCAAT TCACTGTTCC TTGCATTCTA AAACCTTAAA TACCAGAAAA CAGCTTTTTC AAAGTTGTTT TCAAAGTTGG CGTATAACAT AGTATCGACG GAGCCGATTT TGAAACCGCG GTGATCACAG GCAGCAACGC TCTGTCATCG TTACAATCAA CATGCTACCC TCCGCGAGAT CATCCGTGTT TCAAACCCGG CAGCTTAGTT GCCGTTCTTC CGAATAGCAT CGGTAACATG AGCAAAGTCT GCCGCCTTAC AACGGCTCTC CCGCTGACGC CGTCCCGGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGAAT TAACGCCGAA TTAATTCGGG GGATCTGGAT TTTAGTACTG GATTTTGGTT TTAGGAATTA GAAATTTTAT TGATAGAAGT ATTTTACAAA TACAAATACA TACTAAGGGT TTCTTATATG CTCAACACAT GAGCGAAACC CTATAGGAAC CCTAATTCCC TTATCTGGGA ACTACTCACA CATTATTATG GAGAAACTCG AGTCAAATCT CGGTGACGGG CAGGACCGGA CGGGGCGGTA CCGGCAGGCT GAAGTCCAGC TGCCAGAAAC CCACGTCATG CCAGTTCCCG TGCTTGAAGC CGGCCGCCCG CAGCATGCCG CGGGGGGCAT ATCCGAGCGC CTCGTGCATG CGCACGCTCG GGTCGTTGGG CAGCCCGATG ACAGCGACCA CGCTCTTGAA GCCCTGTGCC TCCAGGGACT TCAGCAGATG GGTGTAGAGC GTGGAGCCCA GTCCCGTCCG CTGGTGGCGG GGGGAGACGT ACACGGTCGA CTCGGCCGTC CAGTCGTAGG CGTTGCGTGC CTTCCAGGGG CCCGCGTAGG CGATGCCGGC GACCTCGCCG TCCACCTCGG CGACGAGCCA GGGATAGCGC TCCCGCAGAC GGACGAGGTC GTCCGTCCAC TCCTGCGGTT CCTGCGGCTC GGTACGGAAG TTGACCGTGC TTGTCTCGAT GTAGTGGTTG ACGATGGTGC AGACCGCCGG CATGTCCGCC TCGGTGGCAC GGCGGATGTC GGCCGGGCGT CGTTCTGGGC TCATGGTAGA CTCGAGAGAG ATAGATTTGT AGAGAGAGAC TGGTGATTTC AGCGTGTCCT CTCCAAATGA AATGAACTTC CTTATATAGA GGAAGGGTCT TGCGAAGGAT AGTGGGATTG TGCGTCATCC CTTACGTCAG TGGAGATATC ACATCAATCC ACTTGCTTTG AAGACGTGGT TGGAACGTCT TCTTTTTCCA CGATGCTCCT CGTGGGTGGG GGTCCATCTT TGGGACCACT GTCGGCAGAG GCATCTTGAA CGATAGCCTT TCCTTTATCG CAATGATGGC ATTTGTAGGT GCCACCTTCC TTTTCTACTG TCCTTTTGAT GAAGTGACAG ATAGCTGGGC AATGGAATCC GAGGAGGTTT CCCGATATTA CCCTTTGTTG AAAAGTCTCA ATAGCCCTTT GGTCTTCTGA GACTGTATCT TTGATATTCT TGGAGTAGAC GAGAGTGTCG TGCTCCACCA TGTTCACATC AATCCACTTG CTTTGAAGAC GTGGTTGGAA CGTCTTCTTT TTCCACGATG CTCCTCGTGG GTGGGGGTCC ATCTTTGGGA CCACTGTCGG CAGAGGCATC TTGAACGATA GCCTTTCCTT TATCGCAATG ATGGCATTTG TAGGTGCCAC CTTCCTTTTC TACTGTCCTT TTGATGAAGT GACAGATAGC TGGGCAATGG AATCCGAGGA GGTTTCCCGA TATTACCCTT TGTTGAAAAG TCTCAATAGC CCTTTGGTCT TCTGAGACTG TATCTTTGAT ATTCTTGGAG TAGACGAGAG TGTCGTGCTC CACCATGTTG GCAAGCTGCT CTAGCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC ATGATTACGA ATTCGAGCTC GGTACCCGGG GATCGGCGCG CCAGATTTGC CTTTTCAATT TCAGAAAGAA TGCTAACCCA CAGATGGTTA GAGAGGCTTA CGCAGCAGGT ATCATCAAGA CGATCTACCC GAGCAATAAT CTCCAGGAAA TCAAATACCT TCCCAAGAAG GTTAAAGATG CAGTCAAAAG ATTCAGGACT AACTGCATCA AGAACACAGA GAAAGATATA TTTCTCAAGA TCAGAAGTAC TATTCCAGTA TGGACGATTC AAGGCTTGCT TCACAAACCA AGGCAAGTAA TAGAGATTGG AGTCTCTAAA AAGGTAGTTC CCACTGAATC AAAGGCCATG GAGTCAAAGA TTCAAATAGA GGACCTAACA GAACTCGCCG TAAAGACTGG CGAACAGTTC ATACAGAGTC TCTTACGACT CAATGACAAG AAGAAAATCT TCGTCAACAT GGTGGAGCAC GACACACTTG TCTACTCCAA AAATATCAAA GATACAGTCT CAGAAGACCA AAGGGCAATT GAGACTTTTC AACAAAGGGT AATATCCGGA AACCTCCTCG GATTCCATTG CCCAGCTATC TGTCACTTTA TTGTGAAGAT AGTGGAAAAG GAAGGTGGCT CCTACAAATG CCATCATTGC GATAAAGGAA AGGCCATCGT TGAAGATGCC TCTGCCGACA GTGGTCCCAA AGATGGACCC CCACCCACGA GGAGCATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC AAAGCAAGTG GATTGATGTG ATATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA TCCTTCGCAA GACCCTTCCT CTATATAAGG AAGTTCATTT CATTTGGAGA GAACACGGGG GACTCCTGCA GGTAGATCGC TCGTCGACAT GGATAAGAAG TACTCTATCG GACTCGATAT CGGAACTAAC TCTGTGGGAT GGGCTGTGAT CACCGATGAG TACAAGGTGC CATCTAAGAA GTTCAAGGTT CTCGGAAACA CCGATAGGCA CTCTATCAAG AAAAACCTTA TCGGTGCTCT CCTCTTCGAT TCTGGTGAAA CTGCTGAGGC TACCAGACTC AAGAGAACCG CTAGAAGAAG GTACACCAGA AGAAAGAACA GGATCTGCTA CCTCCAAGAG ATCTTCTCTA ACGAGATGGC TAAAGTGGAT GATTCATTCT TCCACAGGCT CGAAGAGTCA TTCCTCGTGG AAGAAGATAA GAAGCACGAG AGGCACCCTA TCTTCGGAAA CATCGTTGAT GAGGTGGCAT ACCACGAGAA GTACCCTACT ATCTACCACC TCAGAAAGAA GCTCGTTGAT TCTACTGATA AGGCTGATCT CAGGCTCATC TACCTCGCTC TCGCTCACAT GATCAAGTTC AGAGGACACT TCCTCATCGA GGGTGATCTC AACCCTGATA ACTCTGATGT GGATAAGTTG TTCATCCAGC TCGTGCAGAC CTACAACCAG CTTTTCGAAG AGAACCCTAT CAACGCTTCA GGTGTGGATG CTAAGGCTAT CCTCTCTGCT AGGCTCTCTA AGTCAAGAAG GCTTGAGAAC CTCATTGCTC AGCTCCCTGG TGAGAAGAAG AACGGACTTT TCGGAAACTT GATCGCTCTC TCTCTCGGAC TCACCCCTAA CTTCAAGTCT AACTTCGATC TCGCTGAGGA TGCAAAGCTC CAGCTCTCAA AGGATACCTA CGATGATGAT CTCGATAACC TCCTCGCTCA GATCGGAGAT CAGTACGCTG ATTTGTTCCT CGCTGCTAAG AACCTCTCTG ATGCTATCCT CCTCAGTGAT ATCCTCAGAG TGAACACCGA GATCACCAAG GCTCCACTCT CAGCTTCTAT GATCAAGAGA TACGATGAGC ACCACCAGGA TCTCACACTT CTCAAGGCTC TTGTTAGACA GCAGCTCCCA GAGAAGTACA AAGAGATTTT CTTCGATCAG TCTAAGAACG GATACGCTGG TTACATCGAT GGTGGTGCAT CTCAAGAAGA GTTCTACAAG TTCATCAAGC CTATCCTCGA GAAGATGGAT GGAACCGAGG AACTCCTCGT GAAGCTCAAT AGAGAGGATC TTCTCAGAAA GCAGAGGACC TTCGATAACG GATCTATCCC TCATCAGATC CACCTCGGAG AGTTGCACGC TATCCTTAGA AGGCAAGAGG ATTTCTACCC ATTCCTCAAG GATAACAGGG AAAAGATTGA GAAGATTCTC ACCTTCAGAA TCCCTTACTA CGTGGGACCT CTCGCTAGAG GAAACTCAAG ATTCGCTTGG ATGACCAGAA AGTCTGAGGA AACCATCACC CCTTGGAACT TCGAAGAGGT GGTGGATAAG GGTGCTAGTG CTCAGTCTTT CATCGAGAGG ATGACCAACT TCGATAAGAA CCTTCCAAAC GAGAAGGTGC TCCCTAAGCA CTCTTTGCTC TACGAGTACT TCACCGTGTA CAACGAGTTG ACCAAGGTTA AGTACGTGAC CGAGGGAATG AGGAAGCCTG CTTTTTTGTC AGGTGAGCAA AAGAAGGCTA TCGTTGATCT CTTGTTCAAG ACCAACAGAA AGGTGACCGT GAAGCAGCTC AAAGAGGATT ACTTCAAGAA AATCGAGTGC TTCGATTCAG TTGAGATTTC TGGTGTTGAG GATAGGTTCA ACGCATCTCT CGGAACCTAC CACGATCTCC TCAAGATCAT TAAGGATAAG GATTTCTTGG ATAACGAGGA AAACGAGGAT ATCTTGGAGG ATATCGTTCT TACCCTCACC CTCTTTGAAG ATAGAGAGAT GATTGAAGAA AGGCTCAAGA CCTACGCTCA TCTCTTCGAT GATAAGGTGA TGAAGCAGTT GAAGAGAAGA AGATACACTG GTTGGGGAAG GCTCTCAAGA AAGCTCATTA ACGGAATCAG GGATAAGCAG TCTGGAAAGA CAATCCTTGA TTTCCTCAAG TCTGATGGAT TCGCTAACAG AAACTTCATG CAGCTCATCC ACGATGATTC TCTCACCTTT AAAGAGGATA TCCAGAAGGC TCAGGTTTCA GGACAGGGTG ATAGTCTCCA TGAGCATATC GCTAACCTCG CTGGATCTCC TGCAATCAAG AAGGGAATCC TCCAGACTGT GAAGGTTGTG GATGAGTTGG TGAAGGTGAT GGGAAGGCAT AAGCCTGAGA ACATCGTGAT CGAAATGGCT AGAGAGAACC AGACCACTCA GAAGGGACAG AAGAACTCTA GGGAAAGGAT GAAGAGGATC GAGGAAGGTA TCAAAGAGCT TGGATCTCAG ATCCTCAAAG AGCACCCTGT TGAGAACACT CAGCTCCAGA ATGAGAAGCT CTACCTCTAC TACCTCCAGA ACGGAAGGGA TATGTATGTG GATCAAGAGT TGGATATCAA CAGGCTCTCT GATTACGATG TTGATCATAT CGTGCCACAG TCATTCTTGA AGGATGATTC TATCGATAAC AAGGTGCTCA CCAGGTCTGA TAAGAACAGG GGTAAGAGTG ATAACGTGCC AAGTGAAGAG GTTGTGAAGA AAATGAAGAA CTATTGGAGG CAGCTCCTCA ACGCTAAGCT CATCACTCAG AGAAAGTTCG ATAACTTGAC TAAGGCTGAG AGGGGAGGAC TCTCTGAATT GGATAAGGCA GGATTCATCA AGAGGCAGCT TGTGGAAACC AGGCAGATCA CTAAGCACGT TGCACAGATC CTCGATTCTA GGATGAACAC CAAGTACGAT GAGAACGATA AGTTGATCAG GGAAGTGAAG GTTATCACCC TCAAGTCAAA GCTCGTGTCT GATTTCAGAA AGGATTTCCA ATTCTACAAG GTGAGGGAAA TCAACAACTA CCACCACGCT CACGATGCTT ACCTTAACGC TGTTGTTGGA ACCGCTCTCA TCAAGAAGTA TCCTAAGCTC GAGTCAGAGT TCGTGTACGG TGATTACAAG GTGTACGATG TGAGGAAGAT GATCGCTAAG TCTGAGCAAG AGATCGGAAA GGCTACCGCT AAGTATTTCT TCTACTCTAA CATCATGAAT TTCTTCAAGA CCGAGATTAC CCTCGCTAAC GGTGAGATCA GAAAGAGGCC ACTCATCGAG ACAAACGGTG AAACAGGTGA GATCGTGTGG GATAAGGGAA GGGATTTCGC TACCGTTAGA AAGGTGCTCT CTATGCCACA GGTGAACATC GTTAAGAAAA CCGAGGTGCA GACCGGTGGA TTCTCTAAAG AGTCTATCCT CCCTAAGAGG AACTCTGATA AGCTCATTGC TAGGAAGAAG GATTGGGACC CTAAGAAATA CGGTGGTTTC GATTCTCCTA CCGTGGCTTA CTCTGTTCTC GTTGTGGCTA AGGTTGAGAA GGGAAAGAGT AAGAAGCTCA AGTCTGTTAA GGAACTTCTC GGAATCACTA TCATGGAAAG GTCATCTTTC GAGAAGAACC CAATCGATTT CCTCGAGGCT AAGGGATACA AAGAGGTTAA GAAGGATCTC ATCATCAAGC TCCCAAAGTA CTCACTCTTC GAACTCGAGA ACGGTAGAAA GAGGATGCTC GCTTCTGCTG GTGAGCTTCA AAAGGGAAAC GAGCTTGCTC TCCCATCTAA GTACGTTAAC TTTCTTTACC TCGCTTCTCA CTACGAGAAG TTGAAGGGAT CTCCAGAAGA TAACGAGCAG AAGCAACTTT TCGTTGAGCA GCACAAGCAC TACTTGGATG AGATCATCGA GCAGATCTCT GAGTTCTCTA AAAGGGTGAT CCTCGCTGAT GCAAACCTCG ATAAGGTGTT GTCTGCTTAC AACAAGCACA GAGATAAGCC TATCAGGGAA CAGGCAGAGA ACATCATCCA TCTCTTCACC CTTACCAACC TCGGTGCTCC TGCTGCTTTC AAGTACTTCG ATACAACCAT CGATAGGAAG AGATACACCT CTACCAAAGA AGTGCTCGAT GCTACCCTCA TCCATCAGTC TATCACTGGA CTCTACGAGA CTAGGATCGA TCTCTCACAG CTCGGTGGTG ATTCAAGGGC TGATCCTAAG AAGAAGAGGA AGGTTTGACT CGAGATATGA AGATGAAGAT GAAATATTTG GTGTGTCAAA TAAAAGTGTG CTGGAATTGC CCTTAAGCTT CGTTGAACAA CGGAAACTCG ACTTGCCTTC CGCACAATAC ATCATTTCTT CTTAGCTTTT TTTCTTCTTC TTCGTTCATA CAGTTTTTTT TTGTTTATCA GCTTACATTT TCTTGAACCG TAGCTTTCGT TTTCTTCTTT TTAACTTTCC ATTCGGAGTT TTTGTATCTT GTTTCATAGT TTGTCCCAGG ATTAGAATGA TTAGGCATCG AACCTTCAAG AATTTGATTG AATAAAACAT CTTCATTCTT AAGATATGAA GATAATCTTC AAAAGGCCCC TGGGAATCTG AAAGAAGAGA AGCAGGCCCA TTTATATGGG AAAGAACAAT AGTATTTCTT ATATAGGCCC ATTTAAGTTG AAAACAATCT TCAAAAGTCC CACATCGCTT AGATAAGAAA ACGAAGCTGA GTTTATATAC AGCTAGAGTC GAAGTAGTGA TTGTTGGGTC ATAACGATAT CTCGTTTTAG AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG AGTCGGTGCT TTTTTTGTCC CTTCGAAGGG CAATTCTGCA GATATCCATC ACACTGGCAT CGCTACCTTA GGACCGTTAT AGTTAATTAC CCTGTTATCC CTATTAATTA AGAGCTCAGA AATCTCAAAA TTCCGGCAGA ACAATTTTGA ATCTCGATCC GTAGAAACGA GACGGTCATT GTTTTAGTTC CACCACGATT ATATTTGAAA TTTACGTGAG TGTGAGTGAG ACTTGCATAA GAAAATAAAA TCTTTAGTTG GGAAAAAATT CAATAATATA AATGGGCTTG AGAAGGAAGC GAGGGATAGG CCTTTTTCTA AAATAGGCCC ATTTAAGCTA TTAACAATCT TCAAAAGTAC CACAGCGCTT AGGTAAAGAA AGCAGCTGAG TTTATATATG GTTAGAGACG AAGTAGTGAT TGTAACGCCA TCGAAAGTAC ACGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG TCCGTTATCA ACTTGAAAAA GTGGCACCGA GTCGGTGCTT TTTTTCTAGA CCCAGCTTTC TTGTACGTTT AAACTATCAG TGTTTGACAG GATATATTGG CGGGTAAACC TAAGAGAAAA GAGCGTTTAT TAGAATAATC GGATATTTAA AAGGGCGTGA AAAGGTTTAT CCGTTCGTCC ATTTGTATGT GCATGCCAAC CACAGGGTTC CCCTCGGGAT CAAAGTACTT TGATCCAACC CCTCCGCTGC TATAGTGCAG TCGGCTTCTG ACGTTCAGTG CAGCCGTCTT CTGAAAACGA CATGTCGCAC AAGTCCTAAG TTACGCGACA GGCTGCCGCC CTGCCCTTTT CCTGGCGTTT TCTTGTCGCG TGTTTTAGTC GCATAAAGTA GAATACTTGC GACTAGAACC GGAGACATTA CGCCATGAAC AAGAGCGCCG CCGCTGGCCT GCTGGGCTAT GCCCGCGTCA GCACCGACGA CCAGGACTTG ACCAACCAAC GGGCCGAACT GCACGCGGCC GGCTGCACCA AGCTGTTTTC CGAGAAGATC ACCGGCACCA GGCGCGACCG CCCGGAGCTG GCCAGGATGC TTGACCACCT ACGCCCTGGC GACGTTGTGA CAGTGACCAG GCTAGACCGC CTGGCCCGCA GCACCCGCGA CCTACTGGAC ATTGCCGAGC GCATCCAGGA GGCCGGCGCG GGCCTGCGTA GCCTGGCAGA GCCGTGGGCC GACACCACCA CGCCGGCCGG CCGCATGGTG TTGACCGTGT TCGCCGGCAT TGCCGAGTTC GAGCGTTCCC TAATCATCGA CCGCACCCGG AGCGGGCGCG AGGCCGCCAA GGCCCGAGGC GTGAAGTTTG GCCCCCGCCC TACCCTCACC CCGGCACAGA TCGCGCACGC CCGCGAGCTG ATCGACCAGG AAGGCCGCAC CGTGAAAGAG GCGGCTGCAC TGCTTGGCGT GCATCGCTCG ACCCTGTACC GCGCACTTGA GCGCAGCGAG GAAGTGACGC CCACCGAGGC CAGGCGGCGC GGTGCCTTCC GTGAGGACGC ATTGACCGAG GCCGACGCCC TGGCGGCCGC CGAGAATGAA CGCCAAGAGG AACAAGCATG AAACCGCACC AGGACGGCCA GGACGAACCG TTTTTCATTA CCGAAGAGAT CGAGGCGGAG ATGATCGCGG CCGGGTACGT GTTCGAGCCG CCCGCGCACG TCTCAACCGT GCGGCTGCAT GAAATCCTGG CCGGTTTGTC TGATGCCAAG CTGGCGGCCT GGCCGGCCAG CTTGGCCGCT GAAGAAACCG AGCGCCGCCG TCTAAAAAGG TGATGTGTAT TTGAGTAAAA CAGCTTGCGT CATGCGGTCG CTGCGTATAT GATGCGATGA GTAAATAAAC AAATACGCAA GGGGAACGCA TGAAGGTTAT CGCTGTACTT AACCAGAAAG GCGGGTCAGG CAAGACGACC ATCGCAACCC ATCTAGCCCG CGCCCTGCAA CTCGCCGGGG CCGATGTTCT GTTAGTCGAT TCCGATCCCC AGGGCAGTGC CCGCGATTGG GCGGCCGTGC GGGAAGATCA ACCGCTAACC GTTGTCGGCA TCGACCGCCC GACGATTGAC CGCGACGTGA AGGCCATCGG CCGGCGCGAC TTCGTAGTGA TCGACGGAGC GCCCCAGGCG GCGGACTTGG CTGTGTCCGC GATCAAGGCA GCCGACTTCG TGCTGATTCC GGTGCAGCCA AGCCCTTACG ACATATGGGC CACCGCCGAC CTGGTGGAGC TGGTTAAGCA GCGCATTGAG GTCACGGATG GAAGGCTACA AGCGGCCTTT GTCGTGTCGC GGGCGATCAA AGGCACGCGC ATCGGCGGTG AGGTTGCCGA GGCGCTGGCC GGGTACGAGC TGCCCATTCT TGAGTCCCGT ATCACGCAGC GCGTGAGCTA CCCAGGCACT GCCGCCGCCG GCACAACCGT TCTTGAATCA GAACCCGAGG GCGACGCTGC CCGCGAGGTC CAGGCGCTGG CCGCTGAAAT TAAATCAAAA CTCATTTGAG TTAATGAGGT AAAGAGAAAA TGAGCAAAAG CACAAACACG CTAAGTGCCG GCCGTCCGAG CGCACGCAGC AGCAAGGCTG CAACGTTGGC CAGCCTGGCA GACACGCCAG CCATGAAGCG GGTCAACTTT CAGTTGCCGG CGGAGGATCA CACCAAGCTG AAGATGTACG CGGTACGCCA AGGCAAGACC ATTACCGAGC TGCTATCTGA ATACATCGCG CAGCTACCAG AGTAAATGAG CAAATGAATA AATGAGTAGA TGAATTTTAG CGGCTAAAGG AGGCGGCATG GAAAATCAAG AACAACCAGG CACCGACGCC GTGGAATGCC CCATGTGTGG AGGAACGGGC GGTTGGCCAG GCGTAAGCGG CTGGGTTGCC TGCCGGCCCT GCAATGGCAC TGGAACCCCC AAGCCCGAGG AATCGGCGTG AGCGGTCGCA AACCATCCGG CCCGGTACAA ATCGGCGCGG CGCTGGGTGA TGACCTGGTG GAGAAGTTGA AGGCCGCGCA GGCCGCCCAG CGGCAACGCA TCGAGGCAGA AGCACGCCCC GGTGAATCGT GGCAAGCGGC CGCTGATCGA ATCCGCAAAG AATCCCGGCA ACCGCCGGCA GCCGGTGCGC CGTCGATTAG GAAGCCGCCC AAGGGCGACG AGCAACCAGA TTTTTTCGTT CCGATGCTCT ATGACGTGGG CACCCGCGAT AGTCGCAGCA TCATGGACGT GGCCGTTTTC CGTCTGTCGA AGCGTGACCG ACGAGCTGGC GAGGTGATCC GCTACGAGCT TCCAGACGGG CACGTAGAGG TTTCCGCAGG GCCGGCCGGC ATGGCCAGTG TGTGGGATTA CGACCTGGTA CTGATGGCGG TTTCCCATCT AACCGAATCC ATGAACCGAT ACCGGGAAGG GAAGGGAGAC AAGCCCGGCC GCGTGTTCCG TCCACACGTT GCGGACGTAC TCAAGTTCTG CCGGCGAGCC GATGGCGGAA AGCAGAAAGA CGACCTGGTA GAAACCTGCA TTCGGTTAAA CACCACGCAC GTTGCCATGC AGCGTACGAA GAAGGCCAAG AACGGCCGCC TGGTGACGGT ATCCGAGGGT GAAGCCTTGA TTAGCCGCTA CAAGATCGTA AAGAGCGAAA CCGGGCGGCC GGAGTACATC GAGATCGAGC TAGCTGATTG GATGTACCGC GAGATCACAG AAGGCAAGAA CCCGGACGTG CTGACGGTTC ACCCCGATTA CTTTTTGATC GATCCCGGCA TCGGCCGTTT TCTCTACCGC CTGGCACGCC GCGCCGCAGG CAAGGCAGAA GCCAGATGGT TGTTCAAGAC GATCTACGAA CGCAGTGGCA GCGCCGGAGA GTTCAAGAAG TTCTGTTTCA CCGTGCGCAA GCTGATCGGG TCAAATGACC TGCCGGAGTA CGATTTGAAG GAGGAGGCGG GGCAGGCTGG CCCGATCCTA GTCATGCGCT ACCGCAACCT GATCGAGGGC GAAGCATCCG CCGGTTCCTA ATGTACGGAG CAGATGCTAG GGCAAATTGC CCTAGCAGGG GAAAAAGGTC GAAAAGGTCT CTTTCCTGTG GATAGCACGT ACATTGGGAA CCCAAAGCCG TACATTGGGA ACCGGAACCC GTACATTGGG AACCCAAAGC CGTACATTGG GAACCGGTCA CACATGTAAG TGACTGATAT AAAAGAGAAA AAAGGCGATT TTTCCGCCTA AAACTCTTTA AAACTTATTA AAACTCTTAA AACCCGCCTG GCCTGTGCAT AACTGTCTGG CCAGCGCACA GCCCAAGAGC TGCAAAAAGC GCCTACCCTT CGGTCGCTGC GCTCCCTACG CCCCGCCGCT TCGCGTCGGC CTATCGCGGC CGCTGGCCGC TCAAAAATGG CTGGCCTACG GCCAGGCAAT CTACCAGGGC GCGGACAAGC CGCGCCGTCG CCACTCGACC GCCGGCGCCC ACATCAAGGC ACCCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCGCAGC CATGACCCAG TCACGTAGCG ATAGCGGAGT GTATACTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCCCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTAT
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