pARNTL(human)-Fluc-TK-Rluc
$70.00
In stock
SKU
PL52453
100μg liquid plasmid(Instantly):$91
| name | pARNTL(human)-Fluc-TK-Rluc | another name | apARNTL(human)-Fluc-TK-Rluc |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Rluc,Fluc | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGGCTCGA CAGATCAACT GGCCGGTACC TGCGGCTCCT CCATTGGTGG GCGGGGAAGG GGGGTTGGGC ACAGCGATTG GTGGGCGGGG GGCCGGGCCT GGGCCGGCGG GGAGCGGATT GGTCGGAAAG TAGGTTAGTG GTGCGACATT TAGGGAAGGC AGAAAGTAGG TCAGGGACGG AGGTGCCTGT TTACCCGCGC CAGATCTGGC CTCGGCGGCC AAGCTTGGCA ATCCGGTACT GTTGGTAAAG CCACCATGGA AGATGCCAAA AACATTAAGA AGGGCCCAGC GCCATTCTAC CCACTCGAAG ACGGGACCGC CGGCGAGCAG CTGCACAAAG CCATGAAGCG CTACGCCCTG GTGCCCGGCA CCATCGCCTT TACCGACGCA CATATCGAGG TGGACATTAC CTACGCCGAG TACTTCGAGA TGAGCGTTCG GCTGGCAGAA GCTATGAAGC GCTATGGGCT GAATACAAAC CATCGGATCG TGGTGTGCAG CGAGAATAGC TTGCAGTTCT TCATGCCCGT GTTGGGTGCC CTGTTCATCG GTGTGGCTGT GGCCCCAGCT AACGACATCT ACAACGAGCG CGAGCTGCTG AACAGCATGG GCATCAGCCA GCCCACCGTC GTATTCGTGA GCAAGAAAGG GCTGCAAAAG ATCCTCAACG TGCAAAAGAA GCTACCGATC ATACAAAAGA TCATCATCAT GGATAGCAAG ACCGACTACC AGGGCTTCCA AAGCATGTAC ACCTTCGTGA CTTCCCATTT GCCACCCGGC TTCAACGAGT ACGACTTCGT GCCCGAGAGC TTCGACCGGG ACAAAACCAT CGCCCTGATC ATGAACAGTA GTGGCAGTAC CGGATTGCCC AAGGGCGTAG CCCTACCGCA CCGCACCGCT TGTGTCCGAT TCAGTCATGC CCGCGACCCC ATCTTCGGCA ACCAGATCAT CCCCGACACC GCTATCCTCA GCGTGGTGCC ATTTCACCAC GGCTTCGGCA TGTTCACCAC GCTGGGCTAC TTGATCTGCG GCTTTCGGGT CGTGCTCATG TACCGCTTCG AGGAGGAGCT ATTCTTGCGC AGCTTGCAAG ACTATAAGAT TCAATCTGCC CTGCTGGTGC CCACACTATT TAGCTTCTTC GCTAAGAGCA CTCTCATCGA CAAGTACGAC CTAAGCAACT TGCACGAGAT CGCCAGCGGC GGGGCGCCGC TCAGCAAGGA GGTAGGTGAG GCCGTGGCCA AACGCTTCCA CCTACCAGGC ATCCGCCAGG GCTACGGCCT GACAGAAACA ACCAGCGCCA TTCTGATCAC CCCCGAAGGG GACGACAAGC CTGGCGCAGT AGGCAAGGTG GTGCCCTTCT TCGAGGCTAA GGTGGTGGAC TTGGACACCG GTAAGACACT GGGTGTGAAC CAGCGCGGCG AGCTGTGCGT CCGTGGCCCC ATGATCATGA GCGGCTACGT TAACAACCCC GAGGCTACAA ACGCTCTCAT CGACAAGGAC GGCTGGCTGC ACAGCGGCGA CATCGCCTAC TGGGACGAGG ACGAGCACTT CTTCATCGTG GACCGGCTGA AGAGCCTGAT CAAATACAAG GGCTACCAGG TAGCCCCAGC CGAACTGGAG AGCATCCTGC TGCAACACCC CAACATCTTC GACGCCGGGG TCGCCGGCCT GCCCGACGAC GATGCCGGCG AGCTGCCCGC CGCAGTCGTC GTGCTGGAAC ACGGTAAAAC CATGACCGAG AAGGAGATCG TGGACTATGT GGCCAGCCAG GTTACAACCG CCAAGAAGCT GCGCGGTGGT GTTGTGTTCG TGGACGAGGT GCCTAAAGGA CTGACCGGCA AGTTGGACGC CCGCAAGATC CGCGAGATTC TCATTAAGGC CAAGAAGGGC GGCAAGATCG CCGTGAATTC TGCTTGCAAG AACTGGTTCA GTAGCTTAAG CCACTTTGTG ATCCACCTTA ACAGCCACGG CTTCCCTCCC GAGGTGGAGG AGCAGGCCGC CGGCACCCTG CCCATGAGCT GCGCCCAGGA GAGCGGCATG GATAGACACC CTGCTGCTTG CGCCAGCGCC AGGATCAACG TCTAACTGCA GTCTAGAGTC GGGGCGGCCG GCCGCTTCGA GCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTTAAATGAG TCTTCGGACC TCGCGGGGGC CGCTTAAGCG GTGGTTAGGG TTTGTCTGAC GCGGGGGGAG GGGGAAGGAA CGAAACACTC TCATTCGGAG GCGGCTCGGG GTTTGGTCTT GGTGGCCACG GGCACGCAGA AGAGCGCCGC GATCCTCTTA AGCACCCCCC CGCCCTCCGT GGAGGCGGGG GTTTGGTCGG CGGGTGGTAA CTGGCGGGCC GCTGACTCGG GCGGGTCGCG CGCCCCAGAG TGTGACCTTT TCGGTCTGCT CGCAGACCCC CGGGCGGCGC CGCCGCGGCG GCGACGGGCT CGCTGGGTCC TAGGCTCCAT GGGGACCGTA TACGTGGACA GGCTCTGGAG CATCCGCACG ACTGCGGTGA TATTACCGGA GACCTTCTGC GGGACGAGCC GGGTCACGCG GCTGACGCGG AGCGTCCGTT GGGCGACAAA CACCAGGACG GGGCACAGGT ACACTATCTT GTCACCCGGA GGCGCGAGGG ACTGCAGGAG CTTCAGGGAG TGGCGCAGCT GCTTCATCCC CGTGGCCCGT TGCTCGCGTT TGCTGGCGGT GTCCCCGGAA GAAATATATT TGCATGTCTT TAGTTCTATG ATGACACAAA CCCCGCCCAG CGTCTTGTCA TTGGCGAATT CGAACACGCA GATGCAGTCG GGGCGGCGCG GTCCCAGGTC CACTTCGCAT ATTAAGGTGA CGCGTGTGGC CTCGAACACC GAGCGACCCT GCAGCGACCC GCTTAAAAGC TTGATTCTTC TGACACAACA GTCTCGAACT TAAGCTGCAG AAGTTGGTCG TGAGGCACTG GGCAGGTAAG TATCAAGGTT ACAAGACAGG TTTAAGGAGA CCAATAGAAA CTGGGCTTGT CGAGACAGAG AAGACTCTTG CGTTTCTGAT AGGCACCTAT TGGTCTTACT GACATCCACT TTGCCTTTCT CTCCACAGGT GTCCACTCCC AGTTCAATTA CAGCTCTTAA GGCTAGAGTA CTTAATACGA CTCACTATAG GCTAGCCACC ATGACTTCGA AAGTTTATGA TCCAGAACAA AGGAAACGGA TGATAACTGG TCCGCAGTGG TGGGCCAGAT GTAAACAAAT GAATGTTCTT GATTCATTTA TTAATTATTA TGATTCAGAA AAACATGCAG AAAATGCTGT TATTTTTTTA CATGGTAACG CGGCCTCTTC TTATTTATGG CGACATGTTG TGCCACATAT TGAGCCAGTA GCGCGGTGTA TTATACCAGA CCTTATTGGT ATGGGCAAAT CAGGCAAATC TGGTAATGGT TCTTATAGGT TACTTGATCA TTACAAATAT CTTACTGCAT GGTTTGAACT TCTTAATTTA CCAAAGAAGA TCATTTTTGT CGGCCATGAT TGGGGTGCTT GTTTGGCATT TCATTATAGC TATGAGCATC AAGATAAGAT CAAAGCAATA GTTCACGCTG AAAGTGTAGT AGATGTGATT GAATCATGGG ATGAATGGCC TGATATTGAA GAAGATATTG CGTTGATCAA ATCTGAAGAA GGAGAAAAAA TGGTTTTGGA GAATAACTTC TTCGTGGAAA CCATGTTGCC ATCAAAAATC ATGAGAAAGT TAGAACCAGA AGAATTTGCA GCATATCTTG AACCATTCAA AGAGAAAGGT GAAGTTCGTC GTCCAACATT ATCATGGCCT CGTGAAATCC CGTTAGTAAA AGGTGGTAAA CCTGACGTTG TACAAATTGT TAGGAATTAT AATGCTTATC TACGTGCAAG TGATGATTTA CCAAAAATGT TTATTGAATC GGACCCAGGA TTCTTTTCCA ATGCTATTGT TGAAGGTGCC AAGAAGTTTC CTAATACTGA ATTTGTCAAA GTAAAAGGTC TTCATTTTTC GCAAGAAGAT GCACCTGATG AAATGGGAAA ATATATCAAA TCGTTCGTTG AGCGAGTTCT CAAAAATGAA CAATAATTCT AGAGCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAA AATCGATAAG GATCCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC G
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