NM_005334.3;CFF; HCF; HCF-1; HCF1; HFC1; MRX3; PPP1R89;VCAF
100μg liquid plasmid(Instantly):$163
| name | pCMV-HCFC1(human)-3×FLAG-SV40-Neo | another name | apCMV-HCFC1(human)-3×FLAG-SV40-Neo |
| Insert Size (bp) | 6108bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG TACCGAGCTC GGATCCGCCA CCATGGCTTC GGCCGTGTCG CCCGCCAACT TGCCAGCGGT GCTTCTGCAG CCCCGCTGGA AGCGAGTGGT GGGCTGGTCG GGTCCGGTGC CACGGCCCCG CCACGGCCAC CGCGCCGTGG CCATCAAGGA GCTCATCGTG GTGTTTGGCG GCGGCAACGA GGGAATAGTG GACGAACTGC ACGTGTACAA CACGGCAACC AACCAGTGGT TCATCCCAGC CGTGAGGGGG GACATTCCCC CTGGGTGTGC AGCCTATGGC TTCGTGTGTG ACGGGACTCG CCTCCTGGTG TTTGGTGGGA TGGTGGAGTA TGGGAAATAC AGCAATGACC TCTACGAACT CCAGGCGAGC CGGTGGGAGT GGAAGAGACT CAAAGCAAAG ACGCCCAAAA ACGGGCCCCC TCCGTGTCCT CGACTCGGGC ACAGCTTCTC CCTTGTGGGC AACAAATGCT ACCTGTTTGG GGGTCTGGCC AATGATAGCG AGGACCCAAA GAACAACATT CCAAGGTACC TGAATGACTT ATATATCCTG GAATTACGGC CAGGCTCTGG AGTGGTAGCC TGGGACATTC CCATCACTTA CGGGGTCCTA CCACCACCCC GGGAGTCACA TACTGCCGTG GTCTACACCG AAAAAGACAA TAAGAAGTCC AAGCTGGTGA TCTACGGCGG GATGAGTGGC TGCAGGCTGG GGGACCTGTG GACCCTAGAT ATTGACACCC TGACGTGGAA TAAGCCCAGT CTCAGCGGGG TGGCGCCTCT TCCTCGCAGT CTCCACTCGG CAACCACCAT CGGAAATAAA ATGTACGTGT TTGGTGGCTG GGTGCCTCTC GTCATGGATG ACGTCAAAGT GGCCACACAC GAGAAGGAGT GGAAGTGTAC CAACACGCTG GCTTGTCTCA ACCTGGATAC CATGGCCTGG GAGACCATCC TGATGGATAC ACTGGAGGAC AACATCCCCC GTGCTCGGGC TGGCCACTGC GCAGTCGCCA TCAACACCCG CCTGTACATT TGGAGTGGGC GTGACGGCTA CCGCAAGGCC TGGAACAACC AGGTCTGCTG CAAGGACCTC TGGTACCTAG AGACAGAAAA GCCACCACCC CCAGCCCGAG TACAACTGGT ACGCGCCAAC ACCAACTCCC TGGAGGTGAG CTGGGGGGCA GTGGCAACAG CCGACAGCTA CCTTCTCCAG CTCCAGAAAT ATGACATTCC TGCCACGGCT GCTACTGCCA CCTCCCCTAC ACCCAATCCG GTCCCATCTG TGCCTGCCAA CCCTCCCAAG AGCCCTGCCC CAGCAGCAGC CGCACCTGCT GTGCAGCCGC TGACCCAAGT AGGCATCACG CTCCTGCCCC AGGCTGCCCC CGCACCCCCG ACCACCACCA CCATCCAGGT CTTGCCAACG GTGCCTGGCA GCTCCATTTC TGTGCCCACC GCAGCCAGGA CTCAAGGTGT CCCTGCTGTT CTCAAAGTGA CCGGTCCTCA GGCTACAACA GGAACTCCAT TGGTCACCAT GCGACCTGCC AGCCAGGCTG GGAAAGCCCC TGTCACCGTG ACCTCCCTTC CCGCCGGAGT GCGGATGGTT GTGCCAACAC AGAGTGCCCA GGGAACGGTG ATTGGCAGTA GCCCACAGAT GAGTGGGATG GCCGCACTGG CCGCTGCGGC CGCTGCCACC CAGAAGATCC CCCCTTCCTC GGCACCCACG GTGCTGAGTG TCCCAGCGGG TACCACCATC GTGAAGACCA TGGCTGTGAC ACCTGGCACT ACCACCCTCC CAGCCACTGT GAAGGTGGCC TCCTCGCCAG TCATGGTGAG CAACCCTGCC ACTCGCATGC TGAAGACTGC AGCCGCCCAG GTGGGGACAT CGGTTTCCTC CGCCACCAAC ACGTCTACCC GCCCTATCAT CACAGTGCAC AAGTCAGGCA CTGTGACAGT GGCCCAGCAA GCCCAGGTGG TGACCACAGT TGTGGGCGGG GTCACCAAGA CCATCACCCT GGTGAAGAGC CCCATCTCTG TCCCAGGAGG CAGTGCTCTG ATTTCCAATC TGGGCAAAGT GATGTCGGTG GTCCAGACCA AACCAGTTCA GACTTCAGCA GTCACAGGCC AGGCGTCCAC GGGTCCTGTG ACTCAGATCA TCCAGACCAA AGGGCCCCTG CCAGCGGGAA CAATCCTGAA GCTGGTGACC TCAGCAGATG GCAAGCCCAC CACCATCATC ACTACCACGC AGGCCAGTGG GGCGGGGACC AAGCCCACCA TCCTGGGCAT CAGCAGCGTC TCCCCCAGTA CCACCAAGCC CGGCACGACC ACCATCATCA AAACCATCCC CATGTCGGCC ATCATCACCC AGGCGGGCGC CACGGGTGTG ACCAGCAGTC CTGGCATCAA GTCCCCCATC ACCATCATCA CCACCAAGGT GATGACTTCA GGAACTGGAG CACCTGCGAA AATCATCACT GCTGTCCCCA AAATTGCCAC TGGCCACGGG CAGCAGGGAG TGACCCAGGT GGTGCTTAAG GGGGCCCCGG GACAGCCAGG CACCATCCTC CGCACTGTGC CCATGGGGGG TGTTCGCCTG GTCACACCCG TCACCGTCTC CGCCGTCAAG CCAGCCGTCA CCACGTTGGT TGTGAAAGGC ACCACAGGTG TCACGACCCT AGGCACAGTG ACAGGCACCG TCTCCACCAG CCTTGCCGGG GCGGGGGGCC ACAGCACTAG TGCTTCCCTG GCCACGCCCA TCACCACCTT GGGCACCATT GCCACCCTCT CAAGCCAGGT GATCAACCCC ACTGCCATCA CTGTGTCGGC CGCACAGACC ACGCTGACAG CGGCAGGCGG GCTCACAACC CCAACCATCA CCATGCAGCC CGTGTCCCAG CCCACCCAGG TAACTCTGAT CACGGCACCT AGTGGGGTGG AGGCCCAGCC TGTGCATGAC CTCCCTGTGT CCATTCTGGC CTCCCCGACT ACAGAACAGC CCACCGCCAC AGTTACCATC GCCGACTCAG GCCAGGGTGA TGTGCAGCCT GGCACTGTCA CCTTGGTGTG CTCCAACCCA CCCTGTGAGA CCCACGAGAC TGGCACCACC AACACGGCCA CCACTACTGT TGTGGCTAAC CTTGGGGGAC ACCCCCAGCC CACCCAAGTG CAGTTCGTCT GTGACAGACA GGAGGCAGCT GCTTCTCTTG TGACCTCGAC TGTGGGCCAG CAGAATGGTA GCGTGGTCCG AGTCTGTTCG AACCCGCCCT GCGAGACCCA CGAGACGGGC ACCACCAACA CCGCCACCAC CGCCACCTCC AACATGGCCG GGCAGCATGG CTGCTCAAAC CCACCCTGCG AGACCCACGA GACGGGCACC ACCAACACTG CCACTACAGC CATGTCGAGC GTCGGCGCCA ACCACCAGCG AGATGCCCGT CGGGCCTGTG CAGCTGGCAC CCCTGCCGTG ATCCGGATCA GTGTGGCCAC TGGGGCGCTG GAGGCAGCCC AGGGCTCTAA GTCCCAGTGC CAAACCCGCC AGACCAGCGC GACCAGCACC ACCATGACTG TGATGGCCAC CGGGGCCCCG TGCTCGGCCG GCCCACTCCT TGGGCCGAGC ATGGCACGGG AGCCCGGGGG CCGCAGCCCT GCTTTTGTGC AGTTGGCCCC TCTGAGCAGC AAAGTCAGGC TGAGCAGCCC AAGCATTAAG GACCTTCCTG CGGGGCGCCA CAGCCATGCG GTCAGCACCG CTGCCATGAC CCGTTCCAGC GTGGGTGCTG GGGAGCCCCG CATGGCACCT GTGTGCGAGA GCCTCCAGGG TGGCTCGCCC AGCACCACAG TGACTGTGAC AGCCCTGGAG GCACTGCTGT GCCCCTCGGC CACCGTGACC CAAGTCTGCT CCAACCCACC ATGTGAGACC CACGAGACAG GCACCACCAA CACCGCCACT ACCTCGAATG CAGGCAGCGC CCAGAGGGTG TGCTCCAACC CGCCATGCGA GACCCACGAG ACGGGCACCA CCCACACGGC CACCACCGCT ACTTCAAACG GGGGCACGGG CCAGCCCGAG GGTGGGCAGC AGCCCCCTGC TGGTCGCCCC TGTGAGACAC ACCAGACCAC TTCCACTGGC ACCACCATGT CGGTCAGCGT GGGTGCCCTG CTTCCCGACG CCACTTCTTC CCACAGGACC GTGGAGTCTG GCCTAGAGGT GGCGGCGGCA CCCAGCGTCA CCCCCCAGGC TGGCACCGCG CTGCTGGCTC CTTTCCCAAC ACAGAGGGTG TGCTCCAACC CCCCCTGTGA GACCCACGAG ACGGGCACCA CTCACACGGC CACCACTGTC ACTTCCAACA TGAGTTCAAA CCAAGACCCC CCACCTGCTG CCAGCGATCA GGGAGAGGTG GAGAGCACCC AGGGCGACAG CGTGAACATC ACCAGCTCCA GTGCCATCAC GACAACCGTG TCCTCCACAC TGACGCGGGC TGTGACCACC GTGACGCAGT CCACACCGGT CCCGGGCCCC TCTGTGCCGC CCCCAGAGGA ACTCCAGGTG TCGCCAGGTC CTCGCCAGCA GCTGCCGCCA CGGCAGCTTC TGCAGTCGGC TTCCACAGCC CTGATGGGGG AGTCCGCCGA GGTCCTGTCA GCCTCCCAGA CCCCTGAGCT CCCGGCCGCC GTGGATCTGA GCAGCACAGG GGAGCCATCT TCGGGCCAGG AGTCTGCCGG CTCTGCGGTG GTGGCCACTG TGGTGGTCCA GCCACCCCCA CCCACACAGT CCGAAGTAGA CCAGTTATCA CTTCCCCAAG AGCTAATGGC CGAGGCCCAA GCTGGCACCA CCACCCTCAT GGTAACGGGG CTCACCCCCG AGGAGCTGGC AGTGACGGCT GCTGCAGAAG CAGCTGCCCA GGCCGCAGCC ACGGAGGAAG CCCAGGCCCT GGCCATCCAG GCGGTGCTCC AGGCCGCGCA GCAGGCCGTC ATGGGCACCG GCGAGCCCAT GGACACCTCC GAGGCAGCAG CAACCGTGAC TCAGGCGGAG CTGGGGCACC TGTCGGCCGA GGGTCAGGAG GGCCAGGCCA CCACCATACC CATTGTGCTG ACACAGCAGG AGCTGGCTGC CCTGGTGCAG CAGCAGCAGC TGCAGGAGGC CCAGGCCCAG CAGCAGCATC ACCACCTCCC CACTGAGGCC CTGGCCCCTG CCGACAGTCT CAACGACCCA GCCATTGAGA GCAATTGCCT CAATGAGCTG GCCGGCACGG TCCCCAGCAC TGTGGCGCTG CTGCCCTCAA CGGCCACTGA GAGCCTGGCT CCATCCAACA CATTTGTGGC CCCCCAGCCG GTTGTGGTGG CCAGCCCAGC CAAGCTGCAG GCTGCAGCTA CCCTGACCGA AGTGGCCAAT GGCATCGAGT CCCTGGGTGT GAAGCCAGAC CTGCCGCCCC CACCCAGCAA AGCCCCCATG AAGAAGGAAA ACCAGTGGTT TGATGTGGGA GTCATTAAGG GCACCAATGT AATGGTGACA CACTATTTCC TGCCACCAGA TGATGCTGTC CCATCAGACG ATGATTTGGG CACCGTCCCT GACTATAACC AGCTGAAGAA GCAGGAGCTG CAGCCAGGCA CAGCCTATAA GTTTCGTGTT GCCGGAATCA ATGCCTGTGG CCGGGGGCCC TTCAGCGAAA TCTCAGCCTT TAAGACGTGC CTGCCTGGTT TCCCAGGGGC CCCTTGTGCC ATTAAAATCA GCAAAAGTCC GGATGGTGCT CACCTCACCT GGGAGCCACC CTCTGTGACC TCCGGCAAGA TTATCGAGTA CTCCGTGTAC CTGGCCATCC AGAGCTCACA GGCTGGGGGC GAGCTCAAGA GCTCCACCCC GGCCCAGCTG GCCTTCATGC GGGTGTACTG CGGGCCCAGC CCCTCCTGCC TGGTGCAGTC CTCCAGCCTT TCCAACGCCC ACATCGACTA CACCACCAAG CCCGCCATCA TCTTCCGCAT CGCCGCCCGC AATGAGAAGG GCTATGGCCC GGCCACACAA GTGAGGTGGC TGCAGGAAAC CAGTAAAGAC AGCTCTGGCA CCAAGCCAGC CAACAAGCGG CCCATGTCCT CTCCAGAAAT GAAATCTGCT CCAAAGAAAT CTAAGGCCGA TGGTCAGGAA TTCTGCAGAT ATCCAGCACA GTGGCGGCCG CTCGAGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGTAGTGAGG GCCCGATATC TCGACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCCTGGAA ACGGGGGAGG CTAACTGAAA CACGGAAGGA GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG GTGTTGGGTC GTTTGTTCAT AAACGCGGGG TTCGGTCCCA GGGCTGGCAC TCTGTCGATA CCCCACCGAG ACCCCATTGG GGCCAATACG CCCGCGTTTC TTCCTTTTCC CCACCCCACC CCCCAAGTTC GGGTGAAGGC CCAGGGCTCG CAGCCAACGT CGGGGCGGCA GGCCCTGCCA TAGCAGATCT GCGCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGCATC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG GGGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT CTCCCGATCC CCTATGGTCG ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATCTGCT CCCTGCTTGT GTGTTGGAGG TCGCTGAGTA GTGCGCGAGC AAAATTTAAG CTACAACAAG GCAAGGCTTG ACCGACAATT GCATGAAGAA TCTGCTTAGG
