PYCm-HSP70-Linker-OVA(chicken)
100μg liquid plasmid(Instantly):$120
100μg liquid plasmid(Instantly):$120
| name | PYCm-HSP70-Linker-OVA(chicken) | another name | aPYCm-HSP70-Linker-OVA(chicken) |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Chicken | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | U6 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCG CCACTATCCA ACTACACTCA TAATGCTTAA AGAAAATTGG CATTATTTTA CCAATTTTTT TAAAACAAAA AAAGAATAAA ATATTTTCTT ATTATTTAGC TAAAATTGTA CTAAATTTTA TACCAAAAAA AATATATATA AATATATGTA ATATATAATA TATATATATA TATATGTATA TATGTATGTA TGTATATATG TATGTATGTA TATATGTATG TATGTATATA TGTATGTATG TATGTATAGT TTATATTTAT TTGGCAAGTT TTTCTGTAAA AATGTTTAAT TAAAAAAAAA AATATATAAC ATGCCAATAT GCACTATTCA TTTTATGGAG GCAATTTTAT GAGTAACCAT TTTTACTTTT TTTTTACATA TGCGCATACT TCGAGTTATA CAATGTTATT GGAGACGCGT AGAGGCCTCG TCTCAGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CTTTTTTTGG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTCAACA TATGCTGATA ACCAACCAGG AGTCTTAATA CAAGTATATG AAGGAGAAAG AGCTTTAACA AAAGATAATA ATTTATTAGG TAAATTTCAT TTAGATGGTA TCCCACCTGC TCCTAGAAAA GTACCACAAA TTGAAGTTAC ATTCGATATT GATGCTAATG GTATTTTAAA TGTTACAGCT GTTGAAAAAA GTACTGGTAA ACAAAACCAT ATTACAATTA CAAATGATAA AGGTAGATTA TCTCCTGAAG AAATTGATAG AATGGTTAAT GATGCTGAAA AATATAAAGC AGAAGATGAA GAAAATAAAA AAAGAATTGA AGCCAGAAAT AGTCTTGAAA ATTATTGCTA TGGTGTTAAA AGTTCATTAG AAGATCAAAA AATTAAAGAA AAATTACAAC CTAATGAAGT TGAAACATGT ATGAAATCTG TTACTTCTAT TCTTGAATGG TTAGAAAAAA ATCAATTAGC TGGAAAGGAT GAATATGAAG CTAAACAAAA AGAAGCTGAA GCTGTATGCT CTCCAATTAT GTCTAAAATA TATCAAGATG CTGGTGCAGC TGCTGGTGGT ATGCCAGGTG GAATGCCAGG TGGAATGCCA GGAGGAATGC CAGGAGGAAT GCCAGGAGGA ATGAACTTCC CAGGTGGAAT GCCAGGAGGA ATGGGAGCAC CCGCTGGAGC CCCAGCGGGC TCGGGACCAA CTGTTGAAGA AGTTGATGGT TCGGGAATGG GCTCCATCGG CGCAGCAAGC ATGGAATTTT GTTTTGATGT ATTCAAGGAG CTCAAAGTCC ACCATGCCAA TGAGAACATC TTCTACTGCC CCATTGCCAT CATGTCAGCT CTAGCCATGG TATACCTGGG TGCAAAAGAC AGCACCAGGA CACAGATAAA TAAGGTTGTT CGCTTTGATA AACTTCCAGG ATTCGGAGAC AGTATTGAAG CTCAGTGTGG CACATCTGTA AACGTTCACT CTTCACTTAG AGACATCCTC AACCAAATCA CCAAACCAAA TGATGTTTAT TCGTTCAGCC TTGCCAGTAG ACTTTATGCT GAAGAGAGAT ACCCAATCCT GCCAGAATAC TTGCAGTGTG TGAAGGAACT GTATAGAGGA GGCTTGGAAC CTATCAACTT TCAAACAGCT GCAGATCAAG CCAGAGAGCT CATCAATTCC TGGGTAGAAA GTCAGACAAA TGGAATTATC AGAAATGTCC TTCAGCCAAG CTCCGTGGAT TCTCAAACTG CAATGGTTCT GGTTAATGCC ATTGTCTTCA AAGGACTGTG GGAGAAAACA TTTAAGGATG AAGACACACA AGCAATGCCT TTCAGAGTGA CTGAGCAAGA AAGCAAACCT GTGCAGATGA TGTACCAGAT TGGTTTATTT AGAGTGGCAT CAATGGCTTC TGAGAAAATG AAGATCCTGG AGCTTCCATT TGCCAGTGGG ACAATGAGCA TGTTGGTGCT GTTGCCTGAT GAAGTCTCAG GCCTTGAGCA GCTTGAGAGT ATAATCAACT TTGAAAAACT GACTGAATGG ACCAGTTCTA ATGTTATGGA AGAGAGGAAG ATCAAAGTGT ACTTACCTCG CATGAAGATG GAGGAAAAAT ACAACCTCAC ATCTGTCTTA ATGGCTATGG GCATTACTGA CGTGTTTAGC TCTTCAGCCA ATCTGTCTGG CATCTCCTCA GCAGAGAGCC TGAAGATATC TCAAGCTGTC CATGCAGCAC ATGCAGAAAT CAATGAAGCA GGCAGAGAGG TGGTAGGGTC AGCAGAGGCT GGAGTGGATG CTGCAAGCGT CTCTGAAGAA TTTAGGGCTG ACCATCCATT CCTCTTCTGT ATCAAGCACA TCGCAACCAA CGCCGTTCTC TTCTTTGGCA GATGTGTTTC CCCTTAAATA AATTAATTTT TTTTTTTTTT TTTTTTTTAT ATTGTTGTTC TGTAGTTCTT CTTTTGTGAA TAGTATTTTT ATATATGTAC TGTTAATTTT AAAATGTGGA ACGCCACGAA CATATTTTCT CGAACAAAAT ATATATTTTT TGTGTCGAAA TATATAAATT TGTGTCGAAT ATATAAATTT GTGTACTTCC TTTTTGTTGT ATGCATGAAA AAAAGGAATA ACATCAAAGG GGGTCGAGTT ATTATGCTCT CTAGACCCTA AAACAAACTT GTACATCACC ACTACTTTAA TCATACGTAA TATCACTACT TTAAGCATAC GTAATATCAC TACTTTAATC ATACGTAATA TCATTAGATG TAATATCATC AGATGTAATA TCATCAGATG TAATATCATC AGATGTAATA TCATTAAATG TGAAAGCGTG TTAGCACTTG GTATCGGTTT AGCTTTCCAG TCATGCTGGC AATCACATGC TAATATGTGA GGTGTGCCCT TTTTAACTAC ACTCAAAATA ACATGATTTA ATTAACATTG GCGAGCAGCA TATCACTTTT GATGCACTAG CTCTGAAATG TGTACAAGTT TTCATTTGCT AAAGTTACAA AAAAAGGGGG AGTTTTTATT TGAGGCAAGT GCGTTAAAAT GGAAAATTAA AAAAATGAAA AAATGAAAAA AAATGAAAAA TTAAAAAAAA TGAAAAAATG AAAGAATGGC AGAATGGTGA AAGAGCGGGA GGTGGCGAAT TCCTTAAGTT TACAATTTAA TATTCATACT TTAAGTATTT TTTGTAGTAT CCTAGATATT GTGCTTTAAA TGCTCACCCC TCAAAGCACC AGTAATATTT TCATCCACTG AAATACCATT AAATTTTCAA AAAAATACTA TGCATATAAT GTTATACATA TAAACATAAA ACGCCATGTA AATCAAAAAA TATATAAAAA TATGTATAAA AATAAATATG CACTAAATAT AAGCTAATTA TGCATAAAAA TTAAAGTGCC CTTTATTAAC TAGTCGTAAT TATTTATATT TCTATGTTAT AAAAAAATCC TCATATAATA ATATAATTAA TATATGTAAT GTTTTTTTTA TTTTATAATT TTAATATAAA ATAATATGTA AATTAATTCA AAAAATAAAT ATAATTGTTG TGAAACAAAA AACGTAATTT TTTCATTTGC CTTCAAAATT TAAATTTATT TTAATATTTC CTAAAATATA TATACTTTGT GTATAAATAT ATAAAAATAT ATATTTGCTT ATAAATAAAT AAAAAATTTT ATAAAACATA GGGGGATCTA TGGTTGGTTC GCTAAACTGC ATCGTCGCTG TGTCCCAGAA CATGGGCATC GGCAAGAACG GGGACCTGCC CTGGCCACCG CTCAGGAACG AATTTAGATA TTTCCAGAGA ATGACCACAA CCTCTTCAGT AGAAGGTAAA CAGAATCTGG TGATTATGGG TAAGAAGACC TGGTTCTCCA TTCCTGAGAA GAATCGACCT TTAAAGGGTA GAATTAATTT AGTTCTCAGC AGAGAACTCA AGGAACCTCC ACAAGGAGCT CATTTTCTTT CCAGAAGTCT AGATGATGCC TTAAAACTTA CTGAACAACC AGAATTAGCA AATAAAGTAG ACATGGTCTG GATAGTTGGT GGCAGTTCTG TTTATAAGGA AGCCATGAAT CACCCAGGCC ATCTTAAACT ATTTGTGACA AGGATCATGC AAGACTTTGA AAGTGACACG TTTTTTCCAG AAATTGATTT GGAGAAATAT AAACTTCTGC CAGAATACCC AGGTGTTCTC TCTGATGTCC AGGAGGAGAA AGGCATTAAG TACAAATTTG AAGTATATGA GAAGAATGAT GCAAGCGGAG GAGGTGGATC TGGTGGAGGT GGAAGTGCAA GCGTGACAGG GGGAATGGCT AGCAAGTGGG ATCAGAAGGG TATGGACATT GCCTATGAGG AGGCGGCCTT AGGTTACAAA GAGGGTGGTG TTCCTATTGG CGGATGTCTT ATCAATAACA AAGACGGAAG TGTTCTCGGT CGTGGTCACA ACATGAGATT TCAAAAGGGA TCCGCCACAC TACATGGTGA GATCTCCACT TTGGAAAACT GTGGGAGATT AGAGGGCAAA GTGTACAAAG ATACCACTTT GTATACGACG CTGTCTCCAT GCGACATGTG TACAGGTGCC ATCATCATGT ATGGTATTCC ACGCTGTGTT GTCGGTGAGA ACGTTAATTT CAAAAGTAAG GGCGAGAAAT ATTTACAAAC TAGAGGTCAC GAGGTTGTTG TTGTTGACGA TGAGAGGTGT AAAAAGATCA TGAAACAATT TATCGATGAA AGACCTCAGG ATTGGTTTGA AGATATTGGT GAGGCTTCGG AACCATTTAA GAACGTCTAC TTGCTACCTC AAACAAACCA ATTGCTGGGT TTGTACACCA TCATCAGAAA TAAGAATACA ACTAGACCTG ATTTCATTTT CTACTCCGAT AGAATCATCA GATTGTTGGT TGAAGAAGGT TTGAACCATC TACCTGTGCA AAAGCAAATT GTGGAAACTG ACACCAACGA AAACTTCGAA GGTGTCTCAT TCATGGGTAA AATCTGTGGT GTTTCCATTG TCAGAGCTGG TGAATCGATG GAGCAAGGAT TAAGAGACTG TTGTAGGTCT GTGCGTATCG GTAAAATTTT AATTCAAAGG GACGAGGAGA CTGCTTTACC AAAGTTATTC TACGAAAAAT TACCAGAGGA TATATCTGAA AGGTATGTCT TCCTATTAGA CCCAATGCTG GCCACCGGTG GTAGTGCTAT CATGGCTACA GAAGTCTTGA TTAAGAGAGG TGTTAAGCCA GAGAGAATTT ACTTCTTAAA CCTAATCTGT AGTAAGGAAG GGATTGAAAA ATACCATGCC GCCTTCCCAG AGGTCAGAAT TGTTACTGGT GCCCTCGACA GAGGTCTAGA TGAAAACAAG TATCTAGTTC CAGGGTTGGG TGACTTTGGT GACAGATACT ACTGTGTTGG TTCGGGAGAG GGCAGAGGAT CCCTGCTAAC ATGCGGTGAT GTCGAGGAGA ATCCTGGCCC AGAATCGATG GACTATAAGG ACCACGACGG AGACTACAAG GATCATGATA TTGATTACAA AGACGATGAC GATAAGATGG CCCCAAAGAA GAAGCGGAAG GTCGGTATCC ACGGAGTCCC AGCAGCCGAC AAGAAGTACA GCATCGGCCT GGACATCGGC ACCAACTCTG TGGGCTGGGC CGTGATCACC GACGAGTACA AGGTGCCCAG CAAGAAATTC AAGGTGCTGG GCAACACCGA CCGGCACAGC ATCAAGAAGA ACCTGATCGG AGCCCTGCTG TTCGACAGCG GCGAAACAGC CGAGGCCACC CGGCTGAAGA GAACCGCCAG AAGAAGATAC ACCAGACGGA AGAACCGGAT CTGCTATCTG CAAGAGATCT TCAGCAACGA GATGGCCAAG GTGGACGACA GCTTCTTCCA CAGACTGGAA GAGTCCTTCC TGGTGGAAGA GGATAAGAAG CACGAGCGGC ACCCCATCTT CGGCAACATC GTGGACGAGG TGGCCTACCA CGAGAAGTAC CCCACCATCT ACCACCTGAG AAAGAAACTG GTGGACAGCA CCGACAAGGC CGACCTGCGG CTGATCTATC TGGCCCTGGC CCACATGATC AAGTTCCGGG GCCACTTCCT GATCGAGGGC GACCTGAACC CCGACAACAG CGACGTGGAC AAGCTGTTCA TCCAGCTGGT GCAGACCTAC AACCAGCTGT TCGAGGAAAA CCCCATCAAC GCCAGCGGCG TGGACGCCAA GGCCATCCTG TCTGCCAGAC TGAGCAAGAG CAGACGGCTG GAAAATCTGA TCGCCCAGCT GCCCGGCGAG AAGAAGAATG GCCTGTTCGG AAACCTGATT GCCCTGAGCC TGGGCCTGAC CCCCAACTTC AAGAGCAACT TCGACCTGGC CGAGGATGCC AAACTGCAGC TGAGCAAGGA CACCTACGAC GACGACCTGG ACAACCTGCT GGCCCAGATC GGCGACCAGT ACGCCGACCT GTTTCTGGCC GCCAAGAACC TGTCCGACGC CATCCTGCTG AGCGACATCC TGAGAGTGAA CACCGAGATC ACCAAGGCCC CCCTGAGCGC CTCTATGATC AAGAGATACG ACGAGCACCA CCAGGACCTG ACCCTGCTGA AAGCTCTCGT GCGGCAGCAG CTGCCTGAGA AGTACAAAGA GATTTTCTTC GACCAGAGCA AGAACGGCTA CGCCGGCTAC ATTGACGGCG GAGCCAGCCA GGAAGAGTTC TACAAGTTCA TCAAGCCCAT CCTGGAAAAG ATGGACGGCA CCGAGGAACT GCTCGTGAAG CTGAACAGAG AGGACCTGCT GCGGAAGCAG CGGACCTTCG ACAACGGCAG CATCCCCCAC CAGATCCACC TGGGAGAGCT GCACGCCATT CTGCGGCGGC AGGAAGATTT TTACCCATTC CTGAAGGACA ACCGGGAAAA GATCGAGAAG ATCCTGACCT TCCGCATCCC CTACTACGTG GGCCCTCTGG CCAGGGGAAA CAGCAGATTC GCCTGGATGA CCAGAAAGAG CGAGGAAACC ATCACCCCCT GGAACTTCGA GGAAGTGGTG GACAAGGGCG CTTCCGCCCA GAGCTTCATC GAGCGGATGA CCAACTTCGA TAAGAACCTG CCCAACGAGA AGGTGCTGCC CAAGCACAGC CTGCTGTACG AGTACTTCAC CGTGTATAAC GAGCTGACCA AAGTGAAATA CGTGACCGAG GGAATGAGAA AGCCCGCCTT CCTGAGCGGC GAGCAGAAAA AGGCCATCGT GGACCTGCTG TTCAAGACCA ACCGGAAAGT GACCGTGAAG CAGCTGAAAG AGGACTACTT CAAGAAAATC GAGTGCTTCG ACTCCGTGGA AATCTCCGGC GTGGAAGATC GGTTCAACGC CTCCCTGGGC ACATACCACG ATCTGCTGAA AATTATCAAG GACAAGGACT TCCTGGACAA TGAGGAAAAC GAGGACATTC TGGAAGATAT CGTGCTGACC CTGACACTGT TTGAGGACAG AGAGATGATC GAGGAACGGC TGAAAACCTA TGCCCACCTG TTCGACGACA AAGTGATGAA GCAGCTGAAG CGGCGGAGAT ACACCGGCTG GGGCAGGCTG AGCCGGAAGC TGATCAACGG CATCCGGGAC AAGCAGTCCG GCAAGACAAT CCTGGATTTC CTGAAGTCCG ACGGCTTCGC CAACAGAAAC TTCATGCAGC TGATCCACGA CGACAGCCTG ACCTTTAAAG AGGACATCCA GAAAGCCCAG GTGTCCGGCC AGGGCGATAG CCTGCACGAG CACATTGCCA ATCTGGCCGG CAGCCCCGCC ATTAAGAAGG GCATCCTGCA GACAGTGAAG GTGGTGGACG AGCTCGTGAA AGTGATGGGC CGGCACAAGC CCGAGAACAT CGTGATCGAA ATGGCCAGAG AGAACCAGAC CACCCAGAAG GGACAGAAGA ACAGCCGCGA GAGAATGAAG CGGATCGAAG AGGGCATCAA AGAGCTGGGC AGCCAGATCC TGAAAGAACA CCCCGTGGAA AACACCCAGC TGCAGAACGA GAAGCTGTAC CTGTACTACC TGCAGAATGG GCGGGATATG TACGTGGACC AGGAACTGGA CATCAACCGG CTGTCCGACT ACGATGTGGA CCATATCGTG CCTCAGAGCT TTCTGAAGGA CGACTCCATC GACAACAAGG TGCTGACCAG AAGCGACAAG AACCGGGGCA AGAGCGACAA CGTGCCCTCC GAAGAGGTCG TGAAGAAGAT GAAGAACTAC TGGCGGCAGC TGCTGAACGC CAAGCTGATT ACCCAGAGAA AGTTCGACAA TCTGACCAAG GCCGAGAGAG GCGGCCTGAG CGAACTGGAT AAGGCCGGCT TCATCAAGAG ACAGCTGGTG GAAACCCGGC AGATCACAAA GCACGTGGCA CAGATCCTGG ACTCCCGGAT GAACACTAAG TACGACGAGA ATGACAAGCT GATCCGGGAA GTGAAAGTGA TCACCCTGAA GTCCAAGCTG GTGTCCGATT TCCGGAAGGA TTTCCAGTTT TACAAAGTGC GCGAGATCAA CAACTACCAC CACGCCCACG ACGCCTACCT GAACGCCGTC GTGGGAACCG CCCTGATCAA AAAGTACCCT AAGCTGGAAA GCGAGTTCGT GTACGGCGAC TACAAGGTGT ACGACGTGCG GAAGATGATC GCCAAGAGCG AGCAGGAAAT CGGCAAGGCT ACCGCCAAGT ACTTCTTCTA CAGCAACATC ATGAACTTTT TCAAGACCGA GATTACCCTG GCCAACGGCG AGATCCGGAA GCGGCCTCTG ATCGAGACAA ACGGCGAAAC CGGGGAGATC GTGTGGGATA AGGGCCGGGA TTTTGCCACC GTGCGGAAAG TGCTGAGCAT GCCCCAAGTG AATATCGTGA AAAAGACCGA GGTGCAGACA GGCGGCTTCA GCAAAGAGTC TATCCTGCCC AAGAGGAACA GCGATAAGCT GATCGCCAGA AAGAAGGACT GGGACCCTAA GAAGTACGGC GGCTTCGACA GCCCCACCGT GGCCTATTCT GTGCTGGTGG TGGCCAAAGT GGAAAAGGGC AAGTCCAAGA AACTGAAGAG TGTGAAAGAG CTGCTGGGGA TCACCATCAT GGAAAGAAGC AGCTTCGAGA AGAATCCCAT CGACTTTCTG GAAGCCAAGG GCTACAAAGA AGTGAAAAAG GACCTGATCA TCAAGCTGCC TAAGTACTCC CTGTTCGAGC TGGAAAACGG CCGGAAGAGA ATGCTGGCCT CTGCCGGCGA ACTGCAGAAG GGAAACGAAC TGGCCCTGCC CTCCAAATAT GTGAACTTCC TGTACCTGGC CAGCCACTAT GAGAAGCTGA AGGGCTCCCC CGAGGATAAT GAGCAGAAAC AGCTGTTTGT GGAACAGCAC AAGCACTACC TGGACGAGAT CATCGAGCAG ATCAGCGAGT TCTCCAAGAG AGTGATCCTG GCCGACGCTA ATCTGGACAA AGTGCTGTCC GCCTACAACA AGCACCGGGA TAAGCCCATC AGAGAGCAGG CCGAGAATAT CATCCACCTG TTTACCCTGA CCAATCTGGG AGCCCCTGCC GCCTTCAAGT ACTTTGACAC CACCATCGAC CGGAAGAGGT ACACCAGCAC CAAAGAGGTG CTGGACGCCA CCCTGATCCA CCAGAGCATC ACCGGCCTGT ACGAGACACG GATCGACCTG TCTCAGCTGG GAGGCGACAA AAGGCCGGCG GCCACGAAAA AGGCCGGCCA GGCAAAAAAG AAAAAGTAAG AATTACCGGT GATCCCGTTT TTCTTACTTA TATATTTATA CCAATTGATT GTATTTATAA CTGTAAAAAT GTGTATGTTG TGTGCATATT TTTTTTTGTG CATGCACATG CATGTAAATA GCTAAAATTA TGAACATTTT ATTTTTTGTT CAGAAAAAAA AAACTTTACA CACATAAAAT GGCTAGTATG AATAGCCATA TTTTATATAA ATTAAATCCT ATGAATTTAT GACCATATTA AAAATTTAGA TATTTATGGA ACATAATATG TTTGAAACAA TAAGACAAAA TTATTATTAT TATTATTATT TTTACTGTTA TAATTATGTT GTCTCTTCAA TGATTCATAA ATAGTTGGAC TTGATTTTTA AAATGTTTAT AATATGATTA GCATAGTTAA ATAAAAAAAG TTGAAAAATT AAAAAAAAAC ATATAAACAC AAATGATGTT TTTTCCTTCA ATTTCGG