pxylA-BmSQS-ScFPP-6×His
$113.00
In stock
SKU
PL52060
100μg liquid plasmid(Instantly):$134
| name | pxylA-BmSQS-ScFPP-6×His | another name | apxylA-BmSQS-ScFPP-6×His |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Erm | Fluorescent labeling | |
| Promoter | xylA | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATCCTGATAT TGTCTGCATT TGCGCCGGAG CAAACCAAAA ACCTGGTGAG ACACGCCTTG AATTAGTAGA AAAGAACTTG AAGATTTTCA AAGGCATCGT TAGTGAAGTC ATGGCGAGCG GATTTGACGG CATTTTCTTA GTCGCGACGC GAGGCTGGAT GGCCTTCCCC ATTATGATTC TTCTCGCTTC CGGCGGCATC GGGATGCCCG CGTTGCAGGC CATGCTGTCC AGGCAGGTAG ATGACGACCA TCAGGGACAG CTTCAAGGAT CGCTCGCGGC TCTTACCAGC CTAACTTCGA TCACTGGACC GCTGATCGTC ACGGCGATTT ATGCCGCCTC GGCGAGCACA TGGAACGGGT TGGCATGGAT TGTAGGCGCC GCCCTATACC TTGTCTGCCT CCCCGCGTTG CGTCGCGGTG CATGGAGCCG GGCCACCTCG ACCTGAATGG AAGCCGGCGG CACCTCGCTA ACGGATTCAC CACTCCAAGA ATTGGAGCCA ATCAATTCTT GCGGAGAACT GTGAATGCGC AAACCAACCC TTGGCAGAAC ATATCCATCG CGTCCGCCAT CTCCAGCAGC CGCACGCGGC GCATCTCGGG CAGCGTTGGG TCCTGGCCAC GGGTGCGCAT GATCGTGCTC CTGTCGTTGA GGACCCGGCT AGGCTGGCGG GGTTGCCTTA CTGGTTAGCA GAATGAATCA CCGATACGCG AGCGAACGTG AAGCGACTGC TGCTGCAAAA CGTCTGCGAC CTGAGCAACA ACATGAATGG TCTTCGGTTT CCGTGTTTCG TAAAGTCTGG AAACGCGGAA GTCAGCGCCC TGCACCATTA TGTTCCGGAT CTGCATCGCA GGATGCTGCT GGCTACCCTG TGGAACACCT ACATCTGTAT TAACGAAGCG CTGGCATTGA CCCTGAGTGA TTTTTCTCTG GTCCCGCCGC ATCCATACCG CCAGTTGTTT ACCCTCACAA CGTTCCAGTA ACCGGGCATG TTCATCATCA GTAACCCGTA TCGTGAGCAT CCTCTCTCGT TTCATCGGTA TCATTACCCC CATGAACAGA AATTCCCCCT TACACGGAGG CATCAAGTGA CCAAACAGGA AAAAACCGCC CTTAACATGG CCCGCTTTAT CAGAAGCCAG ACATTAACGC TTCTGGAGAA ACTCAACGAG CTGGACGCGG ATGAACAGGC AGACATCTGT GAATCGCTTC ACGACCACGC TGATGAGCTT TACCGCAGCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA CCCAGTCACG TAGCGATAGC GGAGTGTATA CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT GCAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ACACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTTCAA GAATGATGGG CTAATGTGTG TTTACGACAA TTCTCACTTC ATACTTTTCC ATCGTCAGGT CGCCTGACAA TATGTCTCCT GTCATTATGT CCTTCACACT CTGATCAAAC GTGACCAGCT GTTTTTCTTC CGTGAAATTC ATGACAAAAA TATAATCATT GTCCTGATCC TGCCTCGCTT GTACGGAGAC GCCTTTTCCG TGCCGAACCG GAAAAACTGG AGAGAGAGAC AGGTCTGTGA TCAGACCCTC ATAGAAATCA CGCTGAAATT GATCCTCCAA ACGCGCGCCG ATAAAATACG CCTTGCCCTG CTGATACTCA TGGCTTGTGA CCGCTGGCGT GCGCGCATAA AAATCTTCTT GATACACCGC TTCCACTGAA GCTGTCTTTA CATCAATCAC GGTTGCATAA TCCTTCATTT CATATATTTG GCTGCGGTAG CTGACAGCGT TTCGATCCTT CGGATACAGG GTGTCCGTTT CAAGAGGCTC AACTCCAAAT ATAGCTTGAA ATCGATATCT CTGCAGTCGC GATGATTAAT TAATTCAGAA CGCTCGGTTG CCGCCGGGCG TTTTTTATGC AGCAATGGCA AGAACGTCCC GGGGAGCTCC TAACTTATAG GGGTAACACT TAAAAAAGAA TCAATAACGA TAGAAACCGC TCCTAAAGCA GGTGCATTTT TTCCTAACGA AGAAGGCAAT AGTTCACATT TATTGTCTAA ATGAGAATGG ACTCTAGAAG AAACTTCGTT TTTAATCGTA TTTAAAACAA TGGGATGAGA TTCAATTATA TGATTTCTCA AGATAACAGC TTCTATATCA AATGTATTAA GGATATTGGT TAATCCAATT CCGATATAAA AGCCAAAGTT TTGAAGTGCA TTTAACATTT CTACATCATT TTTATTTGCG CGTTCCACAA TCTCTTTTCG AGAAATATTC TTTTCTTCTT TAGAGAGCGA AGCCAGTAAC GCTTTTTCAG AAGCATATAA TTCCCAACAG CCTCGATTTC CACAGCTGCA TTTGGGTCCA TTAAAATCTA TCGTCATATG ACCCATTTCC CCAGAAAAAC CCTGAACACC TTTATACAAT TCGTTGTTAA TAACAAGTCC AGTTCCAATT CCGATATTAA TACTGATGTA AACGATGTTT TCATAGTTTT TTGTCATACC AAATACTTTT TCACCGTATG CTCCTGCATT AGCTTCATTT TCAACAAAAA CCGGAACATT AAACTCACTC TCAATTAAAA ACTGCAAATC TTTGATATTC CAATTTAAGT TAGGCATGAA AATAATTTGC TGATGACGAT CTACAAGGCC TGGAACACAA ATTCCTATTC CGACTAGACC ATAAGGGGAC TCAGGCATAT GGGTTACAAA ACCATGAATA AGTGCAAATA AAATCTCTTT TACTTCACTA GCGGAAGAAC TAGACAAGTC AGAAGTCTTC TCGAGAATAA TATTTCCTTC TAAGTCGGTT AGAATTCCGT TAAGATAGTC GACTCCTATA TCAATACCAA TCGAGTAGCC TGCATTCTTA TTAAAAACAA GCATTACAGG TCTTCTGCCG CCTCTAGATT GCCCTGCCCC AATTTCAAAA ATAAAATCTT TTTCAAGCAG TGTATTTACT TGAGAGGAGA CAGTAGACTT GTTTAATCCT GTAATCTCAG AGAGAGTTGC CCTGGAGACA GGGGAGTTCT TCAAAATTTC ATCTAATATT AATTTTTGAT TCATTTTTTT TACTAAAGCT TGATCTGCAA TTTGAATAAT AACCACTCCT TTGTTTATCC ACCGAACTAA GTTGGTGTTT TTTGAAGCTT GAATTAGATA TTTAAAAGTA TCATATCTAA TATTATAACT AAATTTTCTA AAAAAAACAT TGAAATAAAC ATTTATTTTG TATATGATGA GATAAAGTTA GTTTATTGGA TAAACAAACT AACTCAATTA AGATAGTTGA TGGATAAACT TGTTCACTTA AATCAAAGGG GGAAATGACA AATGGTCCAA ACTAGTGATA TCTAAAAATC AAAGGGGGAA ATGGGATCCG GTACCATTCT GCTAGCAGAT GTCTGTTCCT AACAAACTTC GTGATAACGC TATGGTTATG CTTAAAGAAA CATCTCGTAC ATTCTTCATC CCTATCTCTC ATCTTCCTGC TGAACTTCAA AACGCTGTTG GCTCTGCTTA CCTTTGCATG CGTGCTATCG ATGAAATCGA AGATCATCCT GAACTTGAAG CTGGCGTTAA ATCTCGTCTT CTTTACGCTA TCTCTGATCT TCTTAAAAAA TCTTTCAACG AAGATGAATA CATGGCTCTT GTTGGCCCTT ACCAAGCTGA TCTTCCTGAT GTTACACTTC AACTTGGCAA CTGGATCGCT CTTTGCCCTA CAGGCGTTGC TGATCAAGTT CTTGATGCTA CATCTATCAT GGCTAAAGGC ATGGCTGATT GGGTTGAAAA AGATTGGCAT ATCCAAAACG AAGCTGATCT TGATGATTAC ACATTCTACG TTGCTGGCCT TGTTGGCGTT ATGCTTAACG ATATCTGGAA ATGGTACGAT GGCACAGAAA CAGATAAAGA ACTTGCTATC GCTTTCGGCC GTGGCCTTCA ATCTGTTAAC ATCCTTCGTA ACACATCTGA AGATTCTGAA CGTGGCGTTT CTTTCTTCCC TAACAACTGG TCTCGTGAAG ATATGTTCGC TTACGCTCGT CGTAACCTTT CTCTTGGCAA AAAATACCTT GAAGATGTTA AATCTCTTCC TATCCTTCAT TTCTGCAAAA TCCCTCTTGC TCTTGCTGAT GGCACACTTA ACGCTCTTAT GAAAGGCAAA GAAAAAATGA CACGTGATGA TGTTAACAAA ACAGTTAACG AAGTTGTTGA TATGCATTAA AGGAGAAATA AGAGGAGGAG AAATATGGCA AGCGAAAAAG AAATCAGAAG AGAAAGATTT CTGAACGTGT TTCCGAAACT GGTTGAAGAA TTAAACGCGA GCCTGCTGGC GTATGGCATG CCGAAAGAAG CATGCGATTG GTATGCACAT TCACTGAATT ATAATACACC GGGCGGCAAA CTGAATCGCG GCCTGTCAGT GGTTGATACG TATGCAATTT TATCTAATAA AACGGTGGAA CAACTGGGAC AAGAAGAATA TGAAAAAGTT GCAATCCTGG GCTGGTGCAT CGAACTGCTG CAGGCATATT TTTTAGTGGC GGATGATATG ATGGATAAAA GCATCACAAG AAGAGGACAA CCTTGCTGGT ATAAAGTGCC GGAAGTGGGA GAAATTGCAA TTAATGATGC GTTTATGCTT GAAGCGGCGA TTTATAAACT GCTGAAAAGC CATTTTCGCA ACGAAAAATA TTATATCGAT ATCACGGAAC TGTTTCATGA AGTTACATTT CAGACAGAAC TGGGCCAATT GATGGATCTG ATTACAGCAC CGGAAGATAA AGTGGATCTT TCAAAATTTA GCCTGAAAAA GCATTCATTT ATCGTGACAT TTAAAACGGC ATATTATTCA TTTTATCTGC CGGTAGCCCT GGCAATGTAT GTTGCAGGCA TTACAGATGA AAAAGATCTG AAACAAGCAA GAGATGTGCT TATCCCGCTG GGCGAATATT TTCAAATTCA AGATGATTAT CTGGATTGCT TTGGAACACC GGAACAGATT GGAAAAATCG GCACAGATAT CCAAGATAAC AAATGTTCAT GGGTTATTAA CAAAGCACTG GAACTGGCAT CAGCAGAACA AAGAAAAACA CTGGATGAAA ATTATGGCAA AAAGGATAGC GTTGCAGAAG CAAAATGCAA AAAGATTTTT AACGATCTGA AAATCGAACA GCTGTATCAT GAATATGAAG AAAGCATCGC AAAAGATCTG AAAGCAAAAA TTTCACAAGT GGATGAATCA AGAGGATTTA AAGCGGATGT ACTGACAGCC TTTCTGAACA AAGTGTATAA AAGAAGCAAA CATCATCATC ATCATCATTA ACTCCCACGC GTCAGCTCCT GTACAGGATG AGCCGCGGGC GGCCGCACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTTCA AGAATTGATC CTCTAGCACA AAAGAAAAAC GAAATGATAC ACCAATCAGT GCAAAAAAAG ATATAATGGG AGATAAGACG GTTCGTGTTC GTGCTGACTT GCACCATATC ATAAAAATCG AAACAGCAAA GAATGGCGGA AACGTAAAAG AAGTTATGGA AATAAGACTT AGAAGCAAAC TTAAGAGTGT GTTGATAGTG CAGTATCTTA AAATTTTGTA TAATAGGAAT TGAAGTTAAA TTAGATGCTA AAAATTTGTA ATTAAGAAGG AGTGATTACA TGAACAAAAA TATAAAATAT TCTCAAAACT TTTTAACGAG TGAAAAAGTA CTCAACCAAA TAATAAAACA ATTGAATTTA AAAGAAACCG ATACCGTTTA CGAAATTGGA ACAGGTAAAG GGCATTTAAC GACGAAACTG GCTAAAATAA GTAAACAGGT AACGTCTATT GAATTAGACA GTCATCTATT CAACTTATCG TCAGAAAAAT TAAAACTGAA TACTCGTGTC ACTTTAATTC ACCAAGATAT TCTACAGTTT CAATTCCCTA ACAAACAGAG GTATAAAATT GTTGGGAGTA TTCCTTACCA TTTAAGCACA CAAATTATTA AAAAAGTGGT TTTTGAAAGC CATGCGTCTG ACATCTATCT GATTGTTGAA GAAGGATTCT ACAAGCGTAC CTTGGATATT CACCGAACAC TAGGGTTGCT CTTGCACACT CAAGTCTCGA TTCAGCAATT GCTTAAGCTG CCAGCGGAAT GCTTTCATCC TAAACCAAAA GTAAACAGTG TCTTAATAAA ACTTACCCGC CATACCACAG ATGTTCCAGA TAAATATTGG AAGCTATATA CGTACTTTGT TTCAAAATGG GTCAATCGAG AATATCGTCA ACTGTTTACT AAAAATCAGT TTCATCAAGC AATGAAACAC GCCAAAGTAA ACAATTTAAG TACCGTTACT TATGAGCAAG TATTGTCTAT TTTTAATAGT TATCTATTAT TTAACGGGAG GAAATAATTC TATGAGTCGC TTTTGTAAAT TTGGAAAGTT ACACGTTACT AAAGGGAATG TAGATAAATT ATTAGGTATA CTACTGACAG CTTCCAAGGA GCTAAAGAGG TCCCTAGACT CTAGACCCGG GGATCTCTGC AGTGAGATCT GGTAATGACT CTCTAGCTTG AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGC TCTAGCTAAG CAGAAGGCCA TCCTGACGGA TGGCCTTTTT GCGTTTCTAC AAACTCTTGT TAACTCTAGA GCTGCCTGCC GCGTTTCGGT GATGAAGATC TTCCCGATGA TTAATTAATT CAGAACGCTC GGTTGCCGCC GGGCGTTTTT TATGCAGCAA TGGCAAGAAC GTTGCTCTAG AGCGGCCGCA TCGATTCACA GTGGCAATCT CCCCCGTATT CGTTTGAAAT GTGCCACATT AACAGCGCCG GGTGATGTCC GTATCGTTCT GCTAATAAGC GGTTGATGTG CCGTGTTTTT TCTCGGTAGA CTTTAGATGT GAGGCAGTGG TTGTGCCTTC CGCCGTGCAG CTGTTTGACG CGGGAGGCAT TGACGCGCAA AACTTCCGGA TAGGTTTGCG ACAGCCAGGC CGGACGGGCT CCGCTCGGCG TTGCTAATAT GACCCGGCCG CCTATACTGT GAATCCGCTC AAAAATATCA TCCAGCCATT CAAATTGATA TACGCCCTCC TCCGGCTCAA GTGCGCTCCA TGCAAAAATG CCGACAGAAA ACGTATTCGT ATGAGAAAGC TTCATCAGTT TGATATCGTC AGCTAAAATA TCGGGCCGAT CCAGCCACTG ATCGGGGTTG TAGTCTCCCC CATGGAGCAT AAATTTTGCT TTTGTTACGT GCGTTTTTTC AAGCTTTGAC ATCACCCC
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