pAAVS1-CMV-amilRFP-EF1a-CopGFP-Puro
$84.00
In stock
SKU
PL51916
100μg liquid plasmid(Instantly):$105
| name | pAAVS1-CMV-amilRFP-EF1a-CopGFP-Puro | another name | apAAVS1-CMV-amilRFP-EF1a-CopGFP-Puro |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene knock-in | Fragment type | ORF,CRISPR |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CAG | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTAACGGC CGCCAGTGTG CTGGAATTCG CCCTTGGTAC CTGCTTTCTC TGACCAGCAT TCTCTCCCCT GGGCCTGTGC CGCTTTCTGT CTGCAGCTTG TGGCCTGGGT CACCTCTACG GCTGGCCCAG ATCCTTCCCT GCCGCCTCCT TCAGGTTCCG TCTTCCTCCA CTCCCTCTTC CCCTTGCTCT CTGCTGTGTT GCTGCCCAAG GATGCTCTTT CCGGAGCACT TCCTTCTCGG CGCTGCACCA CGTGATGTCC TCTGAGCGGA TCCTCCCCGT GTCTGGGTCC TCTCCGGGCA TCTCTCCTCC CTCACCCAAC CCCATGCCGT CTTCACTCGC TGGGTTCCCT TTTCCTTCTC CTTCTGGGGC CTGTGCCATC TCTCGTTTCT TAGGATGGCC TTCTCCGACG GATGTCTCCC TTGCGTCCCG CCTCCCCTTC TTGTAGGCCT GCATCATCAC CGTTTTTCTG GACAACCCCA AAGTACCCCG TCTCCCTGGC TTTAGCCACC TCTCCATCCT CTTGCTTTCT TTGCCTGGAC ACCCCGTTCT CCTGTGGATT CGGGTCACCT CTCACTCCTT TCATTTGGGC AGCTCCCCTA CCCCCCTTAC CTCTCTAGTC TGTGCTAGCT CTTCCAGCCC CCTGTCATGG CATCTTCCAG GGGTCCGAGA GCTCAGCTAG TCTTCTTCCT CCAACCCGGG CCCCTATGTC CACTTCAGGA CAGCATGTTT GCTGCCTCCA GGGATCCTGT GTCCCCGAGC TGGGACCACC TTATATTCCC AGGGCCGGTT AATGTGGCTC TGGTTCTGGG TACTTTTATC TGTCCCCTCC ACCCCACAGT GGGGCACGCG TAAGGGCGAA TTCTGCAGAT ATCCATCACA CTGGCGGCCG CTCGAGCATG CATCTAGTAT TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC GTTTAGTGAA CCGTCAGATC GCCTGGAGAC GCCATCCACG CTGTTTTGAC CTCCATAGAA GATTCTAGAA TGGCCCTTAG TAAGCACGGC CTGACTAAGG ACATGACCAT GAAGTACCAT ATGGAGGGCT CGGTGGACGG GCACAAGTTC GTGATTACGG GCCACGGGAA CGGCAACCCA TTCGAGGGGA AACAGACAAT GAACCTGTGT GTGGTGGAAG GCGGACCCCT GCCCTTCTCG GAGGACATCC TGTCCGCAAC CTTTGACTAC GGCAATCGCG TGTTCACAGA GTATCCACAG GGTATGGTGG ATTTCTTTAA GAACAGCTGT CCCGCAGGAT ACACCTGGCA CCGCAGCCTG CTCTTCGAGG ACGGGGCGGT GTGTACCACC TCCGCTGATA TCACCGTGTC CGTCGAGGAG AACTGCTTCT ACCACAACAG CAAATTCCAC GGCGTGAACT TTCCTGCCGA CGGGCCTGTG ATGAAGAAAA TGACTACGAA TTGGGAGCCC AGCTGCGAGA AAATCATCCC GGTCCCTCGT CAGGGAATCT TGAAGGGCGA CATCGCAATG TACCTCCTGC TCAAGGACGG GGGAAGATAC CGATGCCAGT TCGATACCAT CTACAAGGCA AAGTCCGATC CCAAGGAGAT GCCAGAGTGG CACTTCATCC AGCACAAACT CACCCGCGAG GACCGCTCAG ATGCCAAGAA CCAGAAGTGG CAACTGGTCG AGCACGCCGT GGCATCCCGT AGCGCCCTGC CAGGGTAATT TAAACGGCGC GCCTTCGAAT TTAAATCGGA TCCCTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGAG ATCTGCGGCC GCAAGGATCT GCGATCGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT TGAACGGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGCTGA AGCTTCGAGG GGCTCGCATC TCTCCTTCAC GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT GAGTCGCGTT CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG CTCAGGTCGA GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG ACTCAGCCGG CTCTCCACGC TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC GTTTTCTGTT CTGCGCCGTT ACAGATCCAA GCTGTGACCG GCGCCTACGC TAGACGCCAC CATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCA AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG GTGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGTGC TGGTGGGCAG CTTCGCCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTTCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGC TGCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCCATCG CCTTCGCCAG ATCCCGCGCT CAGTCGTCCA ATTCTGCCGT GGACGGCACC GCCGGACCCG GCTCCACCGG ATCTCGCGAG GGCAGAGGAA GTCTTCTAAC ATGCGGTGAC GTGGAGGAGA ATCCCGGCCC TATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG AAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCAGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG AATTGACTCA AATGATGTCA ATTAGTCTAT CAGAAGCTCA TCTGGTCTCC CTTCCGGGGG ACAAGACATC CCTGTTTAAT ATTTAAACAG CAGTGTTCCC AAACTGGGTT CTTATATCCC TTGCTCTGGT CAACCAGGTT GCAGGGTTTC CTGTCCTCAC AGGAACGAAG TCCCTAAAGA AACAGTGGCA GCCAGGTTTA GCCCCGGAAT TGACTGGATT CCTTTTTTAG GGCCCATTGG TATGGTGTAC ACTACTAGGG ACAGGATTGG TGACAGAAAA GCCCCATCCT TAGGCCTCCT CCTTCCTAGT CTCCTGATAT TGGGTCTAAC CCCCACCTCC TGTTAGGCAG ATTCCTTATC TGGTGACACA CCCCCATTTC CTGGAGCCAT CTCTCTCCTT GCCAGAACCT CTAAGGTTTG CTTACGATGG AGCCAGAGAG GATCCTGGGA GGGAGAGCTT GGCAGGGGGT GGGAGGGAAG GGGGGGATGC GTGACCTGCC CGGTTCTCAG TGGCCACCCT GCGCTACCCT CTCCCAGAAC CTGAGCTGCT CTGACGCGGC TGTCTGGTGC GTTTCACTGA TCCTGGTGCT GCAGCTTCCT TACACTTCCC AAGAGGAGAA GCAGTTTGGA AAAACAAAAT CAGAATAAGT TGGTCCTGAG TTCTAACTTT GGCTCTTCAC CTTTCTAGTC CCCAATTTAT ATTGTTCCTC CGTGCGTCAG TTTTACCTGT GAGATAAGGC CAGTAGCCAG CCCCGTCCTG GCAGGGCTGT GGTGAGGAGG GGGGTGTCCG TGTGGAAAAC TCCCTTTGTG AGAATGGTGC GTCCTAGGTG TTCACCAGGT CGTGGCCGCC TCTACTCCCT TTCTCTTTCT CCATCCTTCT TTCCTTAAAG AGTCCCCAGT GCTATCTGGG ACATATTCCT CCGCCCAGAG CAGGGTCCCG CTTCCCTAAG GCCCTGCTCT GGGCTTCTGG GTTTGAGTCC TTGGCAAGCC CAGGAGAGGC GCTCAGGCTT CCCTGTCCCC CTTCCTCGTC CACCATCTCA TGCCCCTGGC TCTCCTGCCC CTTCCCTACA GGTGTTCCTG GCTCTGCTCT CTCGAGATGC ATGCGTCAAT TTTACGCAGA CTATCTTTCT AGGGTTAATC TAGCTGCATC AGGATCATAT CGTCGGGTCT TTTTTCCGGC TCAGTCATCG CCCAAGCTGG CGCTATCTGG GCATCGGGGA GGAAGAAGCC CGTGCCTTTT CCCGCGAGGT TGAAGCGGCA TGGAAAGAGT TTGCCGAGGA TGACTGCTGC TGCATTGACG TTGAGCGAAA ACGCACGTTT ACCATGATGA TTCGGGAAGG TGTGGCCATG CACGCCTTTA ACGGTGAACT GTTCGTTCAG GCCACCTGGG ATACCAGTTC GTCGCGGCTT TTCCGGACAC AGTTCCGGAT GGTCAGCCCG AAGCGCATCA GCAACCCGAA CAATACCGGC GACAGCCGGA ACTGCCGTGC CGGTGTGCAG ATTAATGACA GCGGTGCGGC GCTGGGATAT TACGTCAGCG AGGACGGGTA TCCTGGCTGG ATGCCGCAGA AATGGACATG GATACCCCGT GAGTTACCCG GCGGGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCAT
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