pCMV-PCDHA7(mouse)-EGFP
$84.00
In stock
SKU
PL51907
NM_009957.2;Cnr4; Crnr4
100μg liquid plasmid(Instantly):$105
| name | pCMV-PCDHA7(mouse)-EGFP | another name | apCMV-PCDHA7(mouse)-EGFP |
| Insert Size (bp) | 2841bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTACATTTAT ATTGGCTCAT GTCCAATATG ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCCTCA CTCTCTTCCG CATCGCTGTC TGCGAGGGCC AGCTGTTGGG CTCGCGGTTG AGGACAAACT CTTCGCGGTC TTTCCAGTAC TCTTGGATCG GAAACCCGTC GGCCTCCGAA CGGTACTCCG CCACCGAGGG ACCTGAGCGA GTCCGCATCG ACCGGATCGG AAAACCTCTC GAGCCACCAT GAGAATAAAA CGTCAGCAAT TTGAAATGGT AAACCTGAGA GGATACAACT GGAAGAGCCA ACAATTACTC CTGTTCCTGA TAATTGTGGC AGCCTGGGAG GCAGGGAGCG GCCAGCTCCA CTACTCCGTC CCAGAGGAAG CCAAACACGG CACCTTCGTG GGCCGCATCG CGCAGGACCT GGGGCTGGAG CTGACGGAGC TGGTGCCCCG CCTGTTCAGG GTGGCGTCCA AGGACCGCGG GGACCTTCTG GAGGTAAATC TTCAGAATGG CATTTTGTTT GTGAATTCTC GCATCGACCG GGAGGAACTG TGCGGGCGGA GCGCGGAGTG TAGCATCCAC CTGGAGGTGA TCGTGGACAG GCCGCTGCAG GTTTTCCACG TGGAGGTGGA GGTGAAGGAC ATTAATGACA ACCCTCCCAT GTTTCCAGCG ACACAAAAGG CTCTGTTTAT CCTGGAGTCC AGACTGCTTG ACTCGCGGTT TCCACTAGAG GGCGCGTCAG ATGCAGATGT CGGTTCCAAT GCTCTGCTGA CTTACAGACT GAGCACCAAT GAACATTTCT CTCTGGATGT ACCGCCCAAC CACGAGCAGG TGAAACCTCT TGGGCTGGTT TTGCGGAAAC CTTTAGACAG AGAAGAAGCT GCAGAGATTC GCTTATTGCT CACGGCCACC GACGGAGGCA AACCGGAGCT GACAGGCACC GTTCAATTAC TCATCACCGT GCTGGATGTC AATGACAATG CTCCAGTTTT TGACAGATCT CTGTATACTG TGAAATTGCC AGAAAACGTT CCAAATGGAA CATTGGTAAT CAAAGTCAAT GCCTCAGATT TGGACGAAGG CGTGAATGGG GACGTTATGT ACTCATTTTC TAGTGATGTT TCTTCAGATA TAAAATCCAA GTTCCACATG GACACTGTGA GTGGAGAGAT TACAGTTATA GGAATTATTG ATTTCGAAGA GAGTAAAGCT TACAAGATTC CATTGGAGGC ACGTGATAAA GGGTTCCCAC AACTGCCTGG ACACTGTACC ATTCTTGTGG AAGTTGTAGA TGCTAATGAC AACGCTCCAC AGTTGACGGT TAGTTCCTTG TCTCTCCCAG TATCCGAGGA CAGTCAACCT GGAAGAGTCG TCACTTTAAT TAGCGTATTT GACCGTGATT CCGGTGCCAA TGGGCAGGTG ACCTGCTCCC TGACTCCTCA TATCCCCTTC AAGCTGGTGT CCACATTCAA AAATTACTAT TCGCTCGTGC TGGACAGTGC TCTGGACCGA GAGACCATCG CTAACTATGA TGTGATTGTG ACCGCTCGCG ATGGCGGTTC GCCCTCCCTG TGGGCCACGG CCAGCGTGTC CGTGGAGGTG GCTGATGTGA ACGACAATGC TCCACTGTTT GCGCAGCCTG AATACACGGT GTTCGTGAAG GAGAACAACC CGCCTGGTGC GCACATCTTT ACAGTGTCAG CGATGGATGC TGATGCGCAG GAGAATGCGC TGGTGTCCTA CTCGCTGGTG GAACGGAGGG TGGGCGAGCG CTTGCTGTCG AGCTATGTGT CTGTGCACGC GGAGAGCGGC AAGGTGTTCG CTCTGCAGCC TCTGGACCAT GAGGAGCTGG AACTGCTGCA GTTCCAGGTG AGCGCGCGGG ATGCTGGTGT GCCTGCCCTG GGCAGCAATG TGACTCTGCA GGTGTTTGTA CTGGACGAGA ACGACAATGC ACCTACCCTG CTGGGGCCTT TGGCTAACGG GGTTGGTGGC ACAATGAATG AGGTAGTATC AAGAGCTTTG GTGCCTGGTC AGGTTGTGGC GAAAGTCCGG GCCGTAGATG AGGACTCTGG ATACAATGCG TGGTTGTCTT TTGAGCTGCA GCCAGTTGCG GGTGGAGTTC GTAGTCCCTT CCGTGTAGGC CTGTACACAG GAGAAATCAG CACGACACGT GCTCTGGAGG AGACAGATGC CTTGCGGCAG TGCTTGTTAG TGTTGGTGAA AGATCACGGA GAACCTTCTC TGACCGCCAC AGCCACGGTG TTGCTTTCTC TGGTAGACAG CGGCCAAACA CAGAAGGTCT CATCTAAGGT GTCAGCTGGA GCCTCCAGAG TGGATCAGAG GCTGGTGGAT GTCAACGTGT ACCTGATCAT CGCCATCTGC GCGGTGTCCA GCCTGTTGGT GCTCACGCTA CTGCTGTACA CAGCGCTGCG CTGTTCGGCG ACGCCCACCG ATGGAGCCTG TGCTCCAGGG AAGCCCATGC TGGTGTGCTC CAGCGCGGTG GGGAGCTGGT CATACTCGCA GCAGAGGCGA CAGAGAGTGT GCTCTGGAGA AGGCCCACCC AAGACCGACC TCATGGCCTT CAGCCCCAGC CTACCTCAGG GTCCCAGCTC TACAGATAAC CCCCGGCAGC CCAACCCTGA CTGGCGCTAC TCTGCCTCGC TAAGAGCAGG CATGCACAGC TCTGTGCACC TGGAGGAGGC TGGCATTCTA CGGGCTGGTC CAGGAGGGCC TGATCAGCAG TGGCCAACAG TATCCAGTGC AACACCAGAA CCTGAGGCAG GAGAGGTGTC CCCTCCGGTG GGCGCCGGTG TCAACAGCAA CAGCTGGACC TTTAAATACG GACCAGGCAA CCCCAAACAG TCCGGTCCCG GTGAGTTGCC AGACAAATTC ATTATCCCAG GATCTCCTGC AATCATCTCC ATCCGGCAGG AGCCTGCTAA CAACCAAATT GACAAAAGCG ATTTTATAAC CTTCGGCAAA AAGGAGGAGA CCAAGAAAAA GAAGAAAAAG AAGAAGGGTA ACAAGACCCA GGAGAAAAAA GAGAAAGGGA ACAGCACGAC GGACAACAGT GACCAGGGTA GTGGATCTAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAGG ATCCCCCGAC CTCGACCTCT GGCTAATAAA GGAAATTTAT TTTCATTGCA ATAGTGTGTT GGAATTTTTT GTGTCTCTCA CTCGGAAGGA CATATGGGAG GGCAAATCAT TTGGTCGAGA TCCCTCGGAG ATCTCTAGCT AGAGGATCGA TCCCCGCCCC GGACGAACTA AACCTGACTA CGACATCTCT GCCCCTTCTT CGCGGGGCAG TGCATGTAAT CCCTTCAGTT GGTTGGTACA ACTTGCCAAC TGAACCCTAA ACGGGTAGCA TATGCTTCCC GGGTAGTAGT ATATACTATC CAGACTAACC CTAATTCAAT AGCATATGTT ACCCAACGGG AAGCATATGC TATCGAATTA GGGTTAGTAA AAGGGTCCTA AGGAACAGCG ATGTAGGTGG GCGGGCCAAG ATAGGGGCGC GATTGCTGCG ATCTGGAGGA CAAATTACAC ACACTTGCGC CTGAGCGCCA AGCACAGGGT TGTTGGTCCT CATATTCACG AGGTCGCTGA GAGCACGGTG GGCTAATGTT GCCATGGGTA GCATATACTA CCCAAATATC TGGATAGCAT ATGCTATCCT AATCTATATC TGGGTAGCAT AGGCTATCCT AATCTATATC TGGGTAGCAT ATGCTATCCT AATCTATATC TGGGTAGTAT ATGCTATCCT AATTTATATC TGGGTAGCAT AGGCTATCCT AATCTATATC TGGGTAGCAT ATGCTATCCT AATCTATATC TGGGTAGTAT ATGCTATCCT AATCTGTATC CGGGTAGCAT ATGCTATCCT AATAGAGATT AGGGTAGTAT ATGCTATCCT AATTTATATC TGGGTAGCAT ATACTACCCA AATATCTGGA TAGCATATGC TATCCTAATC TATATCTGGG TAGCATATGC TATCCTAATC TATATCTGGG TAGCATAGGC TATCCTAATC TATATCTGGG TAGCATATGC TATCCTAATC TATATCTGGG TAGTATATGC TATCCTAATT TATATCTGGG TAGCATAGGC TATCCTAATC TATATCTGGG TAGCATATGC TATCCTAATC TATATCTGGG TAGTATATGC TATCCTAATC TGTATCCGGG TAGCATATGC TATCCTCATG ATAAGCTGTC AAACATGAGA ATTAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGC
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