pAAVS1-CMV-CLDN18(human)-2-3×FLAG-EF1a-CopGFP-Puro
$84.00
In stock
SKU
PL51877
NM_001002026.3;SFTA5; SFTPJ
100μg liquid plasmid(Instantly):$105
| name | pAAVS1-CMV-CLDN18(human)-2-3×FLAG-EF1a-CopGFP-Puro | another name | apAAVS1-CMV-CLDN18(human)-2-3×FLAG-EF1a-CopGFP-Puro |
| Insert Size (bp) | 786bp | Plasmid host | Mammary cell |
| Plasmid uses | Gene knock-in | Fragment type | ORF,CRISPR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CAG | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTAACGGC CGCCAGTGTG CTGGAATTCG CCCTTGGTAC CTGCTTTCTC TGACCAGCAT TCTCTCCCCT GGGCCTGTGC CGCTTTCTGT CTGCAGCTTG TGGCCTGGGT CACCTCTACG GCTGGCCCAG ATCCTTCCCT GCCGCCTCCT TCAGGTTCCG TCTTCCTCCA CTCCCTCTTC CCCTTGCTCT CTGCTGTGTT GCTGCCCAAG GATGCTCTTT CCGGAGCACT TCCTTCTCGG CGCTGCACCA CGTGATGTCC TCTGAGCGGA TCCTCCCCGT GTCTGGGTCC TCTCCGGGCA TCTCTCCTCC CTCACCCAAC CCCATGCCGT CTTCACTCGC TGGGTTCCCT TTTCCTTCTC CTTCTGGGGC CTGTGCCATC TCTCGTTTCT TAGGATGGCC TTCTCCGACG GATGTCTCCC TTGCGTCCCG CCTCCCCTTC TTGTAGGCCT GCATCATCAC CGTTTTTCTG GACAACCCCA AAGTACCCCG TCTCCCTGGC TTTAGCCACC TCTCCATCCT CTTGCTTTCT TTGCCTGGAC ACCCCGTTCT CCTGTGGATT CGGGTCACCT CTCACTCCTT TCATTTGGGC AGCTCCCCTA CCCCCCTTAC CTCTCTAGTC TGTGCTAGCT CTTCCAGCCC CCTGTCATGG CATCTTCCAG GGGTCCGAGA GCTCAGCTAG TCTTCTTCCT CCAACCCGGG CCCCTATGTC CACTTCAGGA CAGCATGTTT GCTGCCTCCA GGGATCCTGT GTCCCCGAGC TGGGACCACC TTATATTCCC AGGGCCGGTT AATGTGGCTC TGGTTCTGGG TACTTTTATC TGTCCCCTCC ACCCCACAGT GGGGCACGCG TAAGGGCGAA TTCTGCAGAT ATCCATCACA CTGGCGGCCG CTCGAGCATG CATCTAGTAT TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC GTTTAGTGAA CCGTCAGATC GCCTGGAGAC GCCATCCACG CTGTTTTGAC CTCCATAGAA GATTCTAGAG CCACCATGGC CGTGACTGCC TGTCAGGGCT TGGGGTTCGT GGTTTCACTG ATTGGGATTG CGGGCATCAT TGCTGCCACC TGCATGGACC AGTGGAGCAC CCAAGACTTG TACAACAACC CCGTAACAGC TGTTTTCAAC TACCAGGGGC TGTGGCGCTC CTGTGTCCGA GAGAGCTCTG GCTTCACCGA GTGCCGGGGC TACTTCACCC TGCTGGGGCT GCCAGCCATG CTGCAGGCAG TGCGAGCCCT GATGATCGTA GGCATCGTCC TGGGTGCCAT TGGCCTCCTG GTATCCATCT TTGCCCTGAA ATGCATCCGC ATTGGCAGCA TGGAGGACTC TGCCAAAGCC AACATGACAC TGACCTCCGG GATCATGTTC ATTGTCTCAG GTCTTTGTGC AATTGCTGGA GTGTCTGTGT TTGCCAACAT GCTGGTGACT AACTTCTGGA TGTCCACAGC TAACATGTAC ACCGGCATGG GTGGGATGGT GCAGACTGTT CAGACCAGGT ACACATTTGG TGCGGCTCTG TTCGTGGGCT GGGTCGCTGG AGGCCTCACA CTAATTGGGG GTGTGATGAT GTGCATCGCC TGCCGGGGCC TGGCACCAGA AGAAACCAAC TACAAAGCCG TTTCTTATCA TGCCTCAGGC CACAGTGTTG CCTACAAGCC TGGAGGCTTC AAGGCCAGCA CTGGCTTTGG GTCCAACACC AAAAACAAGA AGATATACGA TGGAGGTGCC CGCACAGAGG ACGAGGTACA ATCTTATCCT TCCAAGCACG ACTATGTGGT GGATCCGAGC TCGGTACCAA GCTTAAGTGA CTACAAGGAT GACGATGACA AGGATTACAA AGACGACGAT GATAAGGACT ATAAGGATGA TGACGACAAA TCTAGATAGT TCGAATTTAA ATCGGATCCC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGAGATCT GCGGCCGCAA GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG TGACCGGCGC CTACGCTAGA CGCCACCATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCAAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGGTGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGTGCTGGT GGGCAGCTTC GCCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT TCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGCTGCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC CCATCGCCTT CGCCAGATCC CGCGCTCAGT CGTCCAATTC TGCCGTGGAC GGCACCGCCG GACCCGGCTC CACCGGATCT CGCGAGGGCA GAGGAAGTCT TCTAACATGC GGTGACGTGG AGGAGAATCC CGGCCCTATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGAAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC AGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGAATT GACTCAAATG ATGTCAATTA GTCTATCAGA AGCTCATCTG GTCTCCCTTC CGGGGGACAA GACATCCCTG TTTAATATTT AAACAGCAGT GTTCCCAAAC TGGGTTCTTA TATCCCTTGC TCTGGTCAAC CAGGTTGCAG GGTTTCCTGT CCTCACAGGA ACGAAGTCCC TAAAGAAACA GTGGCAGCCA GGTTTAGCCC CGGAATTGAC TGGATTCCTT TTTTAGGGCC CATTGGTATG GTGTACACTA CTAGGGACAG GATTGGTGAC AGAAAAGCCC CATCCTTAGG CCTCCTCCTT CCTAGTCTCC TGATATTGGG TCTAACCCCC ACCTCCTGTT AGGCAGATTC CTTATCTGGT GACACACCCC CATTTCCTGG AGCCATCTCT CTCCTTGCCA GAACCTCTAA GGTTTGCTTA CGATGGAGCC AGAGAGGATC CTGGGAGGGA GAGCTTGGCA GGGGGTGGGA GGGAAGGGGG GGATGCGTGA CCTGCCCGGT TCTCAGTGGC CACCCTGCGC TACCCTCTCC CAGAACCTGA GCTGCTCTGA CGCGGCTGTC TGGTGCGTTT CACTGATCCT GGTGCTGCAG CTTCCTTACA CTTCCCAAGA GGAGAAGCAG TTTGGAAAAA CAAAATCAGA ATAAGTTGGT CCTGAGTTCT AACTTTGGCT CTTCACCTTT CTAGTCCCCA ATTTATATTG TTCCTCCGTG CGTCAGTTTT ACCTGTGAGA TAAGGCCAGT AGCCAGCCCC GTCCTGGCAG GGCTGTGGTG AGGAGGGGGG TGTCCGTGTG GAAAACTCCC TTTGTGAGAA TGGTGCGTCC TAGGTGTTCA CCAGGTCGTG GCCGCCTCTA CTCCCTTTCT CTTTCTCCAT CCTTCTTTCC TTAAAGAGTC CCCAGTGCTA TCTGGGACAT ATTCCTCCGC CCAGAGCAGG GTCCCGCTTC CCTAAGGCCC TGCTCTGGGC TTCTGGGTTT GAGTCCTTGG CAAGCCCAGG AGAGGCGCTC AGGCTTCCCT GTCCCCCTTC CTCGTCCACC ATCTCATGCC CCTGGCTCTC CTGCCCCTTC CCTACAGGTG TTCCTGGCTC TGCTCTCTCG AGATGCATGC GTCAATTTTA CGCAGACTAT CTTTCTAGGG TTAATCTAGC TGCATCAGGA TCATATCGTC GGGTCTTTTT TCCGGCTCAG TCATCGCCCA AGCTGGCGCT ATCTGGGCAT CGGGGAGGAA GAAGCCCGTG CCTTTTCCCG CGAGGTTGAA GCGGCATGGA AAGAGTTTGC CGAGGATGAC TGCTGCTGCA TTGACGTTGA GCGAAAACGC ACGTTTACCA TGATGATTCG GGAAGGTGTG GCCATGCACG CCTTTAACGG TGAACTGTTC GTTCAGGCCA CCTGGGATAC CAGTTCGTCG CGGCTTTTCC GGACACAGTT CCGGATGGTC AGCCCGAAGC GCATCAGCAA CCCGAACAAT ACCGGCGACA GCCGGAACTG CCGTGCCGGT GTGCAGATTA ATGACAGCGG TGCGGCGCTG GGATATTACG TCAGCGAGGA CGGGTATCCT GGCTGGATGC CGCAGAAATG GACATGGATA CCCCGTGAGT TACCCGGCGG GCGCGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC AT
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