pLIVE-SEAP
$84.00
In stock
SKU
PL51870
100μg liquid plasmid(Instantly):$105
| name | pLIVE-SEAP | another name | apLIVE-SEAP |
| Insert Size (bp) | 1503bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Albumin | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTAATTAAGA TCTTTTTGAT GGCAGAGTTC AGTTTACCGG GTCACATTGT ACCTGGGAAG ATTCAAGGAT TTATGGAAAA AGTCAACAAC AGGAGTCAGA GCAGCCGGAA AAGCATGGAC TCTGTACTTA GGACTGCGCT TTGAGCAATG GCACAGCAAG CTTTAACCCT GTTTGCAGTC AGCACACAAA CTGTGGTTCA AAGCTCCACT TTATCTCTTC TTGTGGAATT CAGATATCAG ATCAGTTTAA ACCTTGCGGC CGCACTAGTG CTCAAATGGG AGACAAAGAG ATTAAGCTCT TATGTAAAAT TTGCTGTTTT ACATAACTTT AATGAATGGA CAAAGTCTTG TGCATGGGGG TGGGGGTGGG GTTAGAGGGG AACAGCTCCA GATGGCAAAC ATACGCAAGG GATTTAGTCA AACAACTTTT TGGCAAAGAT GGTATGATTT TGTAATGGGG TAGGAACCAA TGAAATGCGA GGTAAGTATG GTTAATAATC TACAGTTATT GGTTAAAGAA GTATATTAGA GCGAGTCTTT CTGCACACAG ATCACCTTCC TATCAACCCC ACTAGCCTCT GGCAAAGGTA CCAGTGTACA GGTTTGTTTC CTTTTTTAAA ATACATTGAG TATGCTTGCC TTTTAGATAT AGAAATATCT GATGCTGTCT TCTTCACTAA ATTTTGATTA CATGATTTGA CAGCAATATT GAAGAGTCTA ACAGCCAGCA CGCAGGTTGG TAAGTACTGT GGGAACATCA CAGATTTTGG CTCCATGCCC TAAAGAGAAA TTGGCTTTCA GATTATTTGG ATTAAAAACA AAGACTTTCT TAAGAGATGT AAAATTTTCA TGATGTTTTC TTTTTTGCTA AAACTAAAGA ATTATTCTTT TACATTTCAG TTTTTCTGCT AGCAGGCGCG CCAGTCGAGA CCATGCTGCT GCTGCTGCTG CTGCTGGGCC TGAGGCTACA GCTCTCCCTG GGCATCATCC CAGTTGAGGA GGAGAACCCG GACTTCTGGA ACCGCGAGGC AGCCGAGGCC CTGGGTGCCG CCAAGAAGCT GCAGCCTGCA CAGACAGCCG CCAAGAACCT CATCATCTTC CTGGGCGATG GGATGGGGGT GTCTACGGTG ACAGCTGCCA GGATCCTAAA AGGGCAGAAG AAGGACAAAC TGGGGCCTGA GATACCCCTG GCCATGGACC GCTTCCCATA TGTGGCTCTG TCCAAGACAT ACAATGTAGA CAAACATGTG CCAGACAGTG GAGCCACAGC CACGGCCTAC CTGTGCGGGG TCAAGGGCAA CTTCCAGACC ATTGGCTTGA GTGCAGCCGC CCGCTTTAAC CAGTGCAACA CGACACGCGG CAACGAGGTC ATCTCCGTGA TGAATCGGGC CAAGAAAGCA GGGAAGTCAG TGGGAGTGGT AACCACCACA CGAGTGCAGC ACGCCTCGCC AGCCGGCACC TACGCCCACA CGGTGAACCG CAACTGGTAC TCGGACGCCG ACGTGCCTGC CTCGGCCCGC CAGGAGGGGT GCCAGGACAT CGCTACGCAG CTCATCTCCA ACATGGACAT TGACGTGATC CTAGGTGGAG GCCGAAAGTA CATGTTTCGC ATGGGAACCC CAGACCCTGA GTACCCAGAT GACTACAGCC AAGGTGGGAC CAGGCTGGAC GGGAAGAATC TGGTGCAGGA ATGGCTGGCG AAGCGCCAGG GTGCCCGGTA TGTGTGGAAC CGCACTGAGC TCATGCAGGC TTCCCTGGAC CCGTCTGTGA CCCATCTCAT GGGTCTCTTT GAGCCTGGAG ACATGAAATA CGAGATCCAC CGAGACTCCA CACTGGACCC CTCCCTGATG GAGATGACAG AGGCTGCCCT GCGCCTGCTG AGCAGGAACC CCCGCGGCTT CTTCCTCTTC GTGGAGGGTG GTCGCATCGA CCATGGTCAT CATGAAAGCA GGGCTTACCG GGCACTGACT GAGACGATCA TGTTCGACGA CGCCATTGAG AGGGCGGGCC AGCTCACCAG CGAGGAGGAC ACGCTGAGCC TCGTCACTGC CGACCACTCC CACGTCTTCT CCTTCGGAGG CTACCCCCTG CGAGGGAGCT CCATCTTCGG GCTGGCCCCT GGCAAGGCCC GGGACAGGAA GGCCTACACG GTCCTCCTAT ACGGAAACGG TCCAGGCTAT GTGCTCAAGG ACGGCGCCCG GCCGGATGTT ACCGAGAGCG AGAGCGGGAG CCCCGAGTAT CGGCAGCAGT CAGCAGTGCC CCTGGACGAA GAGACCCACG CAGGCGAGGA CGTGGCGGTG TTCGCGCGCG GCCCGCAGGC GCACCTGGTT CACGGCGTGC AGGAGCAGAC CTTCATAGCG CACGTCATGG CCTTCGCCGC CTGCCTGGAG CCCTACACCG CCTGCGACCT GGCGCCCCCC GCCGGCACCA CCGACGCCGC GCACCCGGGT TACTCTAGAG TCGGGGCGGC CGGCCGCTTC GAGCAGACAT GACTCGACTC CCGGGATCCA GAGCTCACCG CGGACTCGAG TAACATCACA TTTAAAAGCA TCTCAGGTAA CTATATTTTG AATTTTTTAA AAAAGTAACT GTAATAGTTA TTATTAAAAT AGCAAAGATT GACCATTTCC AAGAGCCATA TAGACCAGCA CCGACCACTA TTCTAAACTA TTTATGTATG TAAATATTAG CTTTTAAAAT TCTCAAAATA GTTGCTGAGT TGGGAACCAC TATTATTTCT ATCGATTCAG CAGCCGTAAG TCTAGGACAG GCTTAAATTG TTTTCACTGG TGTAAATTGC AGAAAGATGA TCTAAGTAAT TTGGCATTTA TTTTAATAGG TTTGAAAAAC ACATGCCATT TTACAAATAA GACTTATATT TGTCCTTTTG TTTTTCAGCC TACCATGAGA ATAAGAGAAA GAAAATGAAG ATCAAAAGCT TATTCATCTG TTTTTCTTTT TCGTTGGTGT AAAGCCAACA CCCTGTCTAA AAAACATAAA TTTCTTTAAT CATTTTGCCT CTTTTCTCTG TGCTTCAATT AATAAAAAAT GGAAAGAATC TAATAGAGTG GTACAGCACT GTTATTTTTC AAAGATGTGT TGCTATCCTG AAAATTCTGT AGGTTCTGTG GAAGTTCCAG TGTTCTCTCT TATTCCACTT CGGTAGAGGA TTTCTAGTTT CTTGTGGGCT AATTAAATAA ATCATTAATA CTCTTCTAAG TTATGGATTA TAAACATTCA AAATAATATT TTGACATTAT GATAATTCTG AATAAAAGAA CAAAAACCAT GGTATAGGTA AGGAATATAA AACATGGCTT TTACCTTAGA AAAAACAATT CTAAAATTCA TATGGAATCA AAAAAGAGCC TGCAGGCGGA ATTGCCAGCT GGGGCGCCCT CTGGTAAGGT TGGGAAGCCC TGCAAATGAC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGTCTAGAG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGGCTCGAC AGATCGCGGC CGCAAGAGAT CA
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
