pCMV-PCDHGA8(mouse)-EGFP
$84.00
In stock
SKU
PL51844
NM_033591.3;mKIAA4054
100μg liquid plasmid(Instantly):$105
| name | pCMV-PCDHGA8(mouse)-EGFP | another name | apCMV-PCDHGA8(mouse)-EGFP |
| Insert Size (bp) | 2799bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTACATTTAT ATTGGCTCAT GTCCAATATG ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCCTCA CTCTCTTCCG CATCGCTGTC TGCGAGGGCC AGCTGTTGGG CTCGCGGTTG AGGACAAACT CTTCGCGGTC TTTCCAGTAC TCTTGGATCG GAAACCCGTC GGCCTCCGAA CGGTACTCCG CCACCGAGGG ACCTGAGCGA GTCCGCATCG ACCGGATCGG AAAACCTCTC GAGCCACCAT GGCTGCTTCC AAGAATCAAC ACAGGCATGG CCGGCTGGTG CTGCTGTGTA CGCTGATGGG GACGCTGTGC AAGATCAGCG TAGGACAGAT TCGCTACTCC GTGCCAGAAG AGACAGACAA AGGCACTGTC GTGGGCAACA TCTCCAAGGA CCTGGGGCTG GAGCCCCGCG AGCTGGCGGA GCGCGGGGTC CGTATCGTCT CCAGAGGTAG GTCGCAGCTT TTCTCCCTGA ATCCGCGGGG CGGCAGCTTG GTCACCGCAG GCAGGATAGA CCGGGAGGAG CTGTGCGCTC AGAGCACGCC TTGTCTTGTA AATATCAACA TCCTAGTTGA GGAGAAAGGA AAACTCTTTG GGGTAGAAAT TGAAATAACT GATATTAACG ATAATAATCC GAAGTTCCAT GTTGGAGATC TGGAGGTGAA AATTAATGAA ATCGCTGCCC CTGGAGCACG GTATCCACTC CCAGAAGCGG TTGACCCAGA TGTGGGCATA AACTCCCTCC AGAGTTACCA GCTCAGCCCC AATCGCCACT TCTCTCTCCA CCTGCAAACT GGAGACGACG GAACTATAAA CCCAGAGCTG GTGCTAGAGC GCACTCTGGA CCGAGAGGAA GAACCCACTC ACCACCTGGT CCTCACGGCC TCCGATGGCG GCGAGCCACG CCGCTCCAGC ACAGCGCTTA TCCAGATCAC AGTGTTGGAT ACTAACGACA ATGCCCCTGT TTTTGACCAA CCGGTTTACC GCGTGAAAGT CCTTGAGAAT GTGGCCCCAG GCACCCTGCT GCTCACAGTA AGAGCTAGCG ACCCGGATGA GGGCGTCAAT GGGAAGGTGA CATACAAATT CCGGAAAATT AATGAAAAGC AGTCGCTGTT GTTCCATCTT CATGAAAACA CGGGAGAAAT GACAGTAGCG AAAAATCTGG ACTATGAAGA ATGTTCATTG TATGAAATGG AAATACAGGC TGAAGACGTA GGGGCGCTTC TGGGGAGGAG CAAAGTGATA ATTATGGTAG AAGATGTAAA TGACAATCGG CCAGAAGTAA CCATTACATC CTTGTTTAAT CCGGTATTGG AAAATTCTCT TCCTGGAACA GTAATCGCCT TCTTGAATGT ACATGACCAA GACTCTGGAA AGAACGGCCA AGTTGTCTGT TACACGCATG ATAACTTACC CTTTAAACTA GAAAAGTCAA TAGATAATTA TTATAGATTG GTAACATGGA AATACTTGGA CCGAGAAAAA GTCTCTACCT ACAATATCAC GGTGATAGCT TCGGACCTAG GAGCCCCACC TCTGTCTACT GAAACTTATA TTGCCCTGAC TGTGGCAGAC ACTAATGACA ACCCTCCTCG TTTTTCTCAC ACTTCCTACA CAGCCTATCT CCCAGAGAAC AACCTGAGAG GTGCCTCCAT CTTCTCATTG ACTGCACATG ACCCTGACAG CCAGGAGAAT GCCCAGGTCA CTTACTCTGT GTCTGAGGAC ACTATACAGG GAGTGCCTTT GTCCTCTTAC ATCTCCATCA ACTCTGACAC TGGCATCCTG TACGCACTGC AATCTTTTGA CTTTGAGAAG ATACAAGACT TGCAGCTATT GGTTATTGCC ACTGACAGTG GAAGCCCACC TCTCAGCAGC AATGTGTCCC TGAGCTTGTT TGTGTTGGAC CAGAATGACA ATGCACCTGA GATCCTATAC CCCAGCTTCC CCACAGATGG TTCCACTGGC GTAGAATTAG CGCCCCGCTC TGCAGAGCCT GGATACCTGG TGACCAAAGT GGTGGCAGTG GACAAAGACT CGGGACAGAA TGCTTGGCTG TCCTACCGTC TGCTCAAGGC CAGCGAACCT GGGCTCTTCT CTGTAGGGCT TCACACGGGT GAGGTGCGTA CAGCGAGGGC CCTGCTGGAC AGAGATGCTC TCAAACAGAA CCTGGTGATG GCTGTGCAGG ACCATGGCCA GCCTCCTCTC TCAGCCACTG TAACTCTCAC TGTGGCCGTG GCTAACAGCA TCCCTGAAGT GTTGGCTGAC TTGAGCAGCA TTATGACCCC TGAGGTACCA GAGGATTCTG ATCTCACGCT CCACCTGGTG GTGGCAGTGG CTGTGGTCTC CTGTGTCTTC CTTGTCTTTG TCATTGTCCT CCTAGCTCTC AGACTTCAGC GCTGGCAGAA GTCTCGCCAG CTCCAGGGCT CCAGAAGTGG ATTGGCCCCT GCACCTCCAT CACATTTTGT GGGCATTGAC GGGGTACAAG CTTTTCTACA AACCTATTCT CATGAAGTCT CACTCACTTC AGGCTCCCAG ACAAGCCACA TTATCTTTCC GCAGCCCAAC TATGCAGACA TGCTCATTAA CCAAGAAAGC TGTGAGAAAA ATGATTCCTT ATTAACATCC ATAGATTTCC ATGAGAATAA GGATGAAGAT GCTTGCGCTC CGCAAGCCCC GCCCAACACT GACTGGCGTT TCTCTCAAGC CCAGAGACCC GGCACGAGCG GATCCCAAAA TGGTGATGAA ACTGGCACCT GGCCCAACAA CCAGTTTGAT ACAGAGATGC TGCAAGCCAT GATCTTGGCC TCTGCCAGCG AAGCTGCTGA TGGGAGCTCT ACCCTGGGAG GGGGCGCTGG CACCATGGGT CTGAGCGCTC GATACGGACC CCAGTTTACC CTGCAGCACG TGCCTGACTA CCGCCAGAAC GTGTACATCC CTGGCAGCAA TGCCACGCTG ACCAATGCCG CTGGCAAACG AGATGGCAAG GCTCCAGCAG GTGGTAATGG CAACAAGAAG AAATCGGGCA AGAAAGAGAA GAAGGGTAGT GGATCTATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAGGAT CCCCCGACCT CGACCTCTGG CTAATAAAGG AAATTTATTT TCATTGCAAT AGTGTGTTGG AATTTTTTGT GTCTCTCACT CGGAAGGACA TATGGGAGGG CAAATCATTT GGTCGAGATC CCTCGGAGAT CTCTAGCTAG AGGATCGATC CCCGCCCCGG ACGAACTAAA CCTGACTACG ACATCTCTGC CCCTTCTTCG CGGGGCAGTG CATGTAATCC CTTCAGTTGG TTGGTACAAC TTGCCAACTG AACCCTAAAC GGGTAGCATA TGCTTCCCGG GTAGTAGTAT ATACTATCCA GACTAACCCT AATTCAATAG CATATGTTAC CCAACGGGAA GCATATGCTA TCGAATTAGG GTTAGTAAAA GGGTCCTAAG GAACAGCGAT GTAGGTGGGC GGGCCAAGAT AGGGGCGCGA TTGCTGCGAT CTGGAGGACA AATTACACAC ACTTGCGCCT GAGCGCCAAG CACAGGGTTG TTGGTCCTCA TATTCACGAG GTCGCTGAGA GCACGGTGGG CTAATGTTGC CATGGGTAGC ATATACTACC CAAATATCTG GATAGCATAT GCTATCCTAA TCTATATCTG GGTAGCATAG GCTATCCTAA TCTATATCTG GGTAGCATAT GCTATCCTAA TCTATATCTG GGTAGTATAT GCTATCCTAA TTTATATCTG GGTAGCATAG GCTATCCTAA TCTATATCTG GGTAGCATAT GCTATCCTAA TCTATATCTG GGTAGTATAT GCTATCCTAA TCTGTATCCG GGTAGCATAT GCTATCCTAA TAGAGATTAG GGTAGTATAT GCTATCCTAA TTTATATCTG GGTAGCATAT ACTACCCAAA TATCTGGATA GCATATGCTA TCCTAATCTA TATCTGGGTA GCATATGCTA TCCTAATCTA TATCTGGGTA GCATAGGCTA TCCTAATCTA TATCTGGGTA GCATATGCTA TCCTAATCTA TATCTGGGTA GTATATGCTA TCCTAATTTA TATCTGGGTA GCATAGGCTA TCCTAATCTA TATCTGGGTA GCATATGCTA TCCTAATCTA TATCTGGGTA GTATATGCTA TCCTAATCTG TATCCGGGTA GCATATGCTA TCCTCATGAT AAGCTGTCAA ACATGAGAAT TAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGC
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