BC047521.1;BANDDOS; C-FMS; CD115; CSF-1R; CSFR; FIM2;FMS; HDLS; M-CSF-R
100μg liquid plasmid(Instantly):$105
| name | pLV2-TRE3GS-mCherry-CSF1R(human)-Myc-FLAG-TetOne-Puro | another name | apLV2-TRE3GS-mCherry-CSF1R(human)-Myc-FLAG-TetOne-Puro |
| Insert Size (bp) | 2919bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Red |
| Promoter | TRE3GS | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTACTAAGT TTGTAGTACA TATTTAACAA ATACAATTTC TTTAAAAACT ACTAAGTTTG TAGTACATAT TTAACAAATA CAATTTCTTT AAAATGAAAA TAATTCAGAG GAATCACAGG TTTAGAGTAA ATGAAACCAC TAGGTAATTG GCAGTGGTAA TAGGGTATGG GGTGGGAAGT TTGGGATGAT TTTGGTTAGC TTGAGTTATC CAGTTGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT GATCTCCCGA TCCGTCGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCT AGCTAGAGGT CGACGGTAAA ACGCTAGCAA TGTCAAGGCC TCTCACTCTC TGATATTCAT TTCTTTGCAA GTTATAAATA CTGAATAATA AGATGACATG AACTACTACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGGCA AAAAGCAGCT GCTTATATGC AGGATCTGAG GGCTCGCCAC TCCCCAGTCC CGCCCAGGCC ACGCCTCCCT GGAAAGTCCC CAGCGGAAAG TCCCTTGTAG CAAGCTCGAT ATCAGCAGTT CTTGAAGTAC TCCGGATGCA GCTCTCGGGC CACGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AACACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCTGGTG TTCTCTCCTT TATTGGCCTC TTCTACCTTA TCTGGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGACCCCT GGCCCTGGTG TGTAGTTCTG CTAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCT CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACTGCAGAA TTAATTCCAG GCGGGGAGGC GGCCCAAAGG GAGATCCGAC TCGTCTGAGG GCGAAGGCGA AGACGCGGAA GAGGCCGCAG AGCCGGCAGC AGGCCGCGGG AAGGAAGGTC CGCTGGATTG AGGGCCGAAG GGACGTAGCA GAAGGACGTC CCGCGCAGAA TCCAGGTGGC AACACAGGCG AGCAGCCATG GAAAGGACGT CAGCTTCCCC GACAACACCA CGGAATTGTC AGTGCCCAAC AGCCGAGCCC CTGTCCAGCA GCGGGCAAGG CAGGCGGCGA TGAGTTCCGC CGTGGCAATA GGGAGGGGGA AAGCGAAAGT CCCGGAAAGG AGCTGACAGG TGGTGGCAAT GCCCCAACCA GTGGGGGTTG CGTCAGCAAA CACAGTGCAC ACCACGCCAC GTTGCCTGAC AACGGGCCAC AACTCCTCAT AAAGAGACAG CAACCAGGAT TTATACAAGG AGGAGAAAAT GAAAGCCATA CGGGAAGCAA TAGCATGATA CAAAGGCATT AAAGCAGCGT ATCCACATAG CGTAAAAGGA GCAACATAGT TAAGAATACC AGTCAATCTT TCACAAATTT TGTAATCCAG AGGTTGATTG TTCCAGACGC GTTCAGGCAC CGGGCTTGCG GGTCATGCAC CAGGTGCGCG GTCCTTCGGG CACCTCGACG TCGGCGGTGA CGGTGAAGCC GAGCCGCTCG TAGAAGGGGA GGTTGCGGGG CGCGGAGGTC TCCAGGAAGG CGGGCACCCC GGCGCGCTCG GCCGCCTCCA CTCCGGGGAG CACGACGGCG CTGCCCAGAC CCTTGCCCTG GTGGTCGGGC GAGACGCCGA CGGTGGCCAG GAACCACGCG GGCTCCTTGG GCCGGTGCGG CGCCAGGAGG CCTTCCATCT GTTGCTGCGC GGCCAGCCGG GAACCGCTCA ACTCGGCCAT GCGCGGGCCG ATCTCGGCGA ACACCGCCCC CGCTTCGACG CTCTCCGGCG TGGTCCAGAC CGCCACCGCG GCGCCGTCGT CCGCGACCCA CACCTTGCCG ATGTCGAGCC CGACGCGCGT GAGGAAGAGT TCTTGCAGCT CGGTGACCCG CTCGATGTGG CGGTCCGGGT CGACGGTGTG GCGCGTGGCG GGGTAGTCGG CGAACGCGGC GGCGAGGGTG CGTACGGCCC GGGGGACGTC GTCGCGGGTG GCGAGGCGCA CCGTGGGCTT GTACTCGGTC ATGGTCGCGT TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC ATTCGCGCGT TTACCCGGGG AGCATGTCAA GGTCAAAATC GTCAAGAGCG TCAGCAGGCA GCATATCAAG GTCAAAGTCG TCAAGGGCAT CGGCTGGGAG CATGTCTAAG TCAAAATCGT CAAGGGCGTC GGTCGGCCCG CCGCTTTCGC ACTTTAGCTG TTTCTCCAGG CCACATATGA TTAGTTCCAG GCCGAAAAGG AAGGCAGGTT CGGCTCCCTG CCGGTCGAAC AGCTCAATTG CTTGTTTCAG AAGTGGGGGC ATAGAATCGG TGGTAGGTGT CTCTCTTTCC TCTTTTGCTA CTTGATGCTC CTGTTCCTCC AATACGCAGC CCAGTGTAAA GTGGCCCACG GCGGACAGAG CGTACAGTGC GTTCTCCAGG GAGAAGCCTT GCTGACACAG GAACGCGAGC TGATTTTCCA GGGTTTCGTA CTGTTTCTCT GTTGGGCGGG TGCCGAGATG CACTTTAGCC CCGTCGCGAT GTGAGAGGAG AGCACAGCGG TATGACTTGG CGTTGTTCCG CAGAAAGTCT TGCCATGACT CGCCTTCCAG GGGGCAGGAG TGGGTATGAT GCCTGTCCAG CATCTCGATT GGCAGGGCAT CGAGCAGGGC CCGCTTGTTC TTCACGTGCC AGTACAGGGT AGGCTGCTCA ACTCCCAGCT TTTGAGCGAG TTTCCTTGTC GTCAGGCCTT CGATACCGAC TCCATTGAGT AATTCCAGAG CAGAGTTTAT GACTTTGCTC TTGTCCAGTC TAGACATGGT AATTCGATGA TCCCCCTGGG GAGAGAGGTC GGTGATTCGG TCAACGAGGG AGCCGACTGC CGACGTGCGC TCCGGAGGCT TGCAGAATGC GGAACACCGC GCGGGCAGGA ACAGGGCCCA CACTACCGCC CCACACCCCG CCTCCCGCAC CGCCCCTTCC CGGCCGCTGC TCTCGGCGCG CCCTGCTGAG CAGCCGCTAT TGGCCACAGC CCATCGCGGT CGGCGCGCTG CCATTGCTCC CTGGCGCTGT CCGTCTGCGA GGGTACTAGT GAGACGTGCG GCTTCCGTTT GTCACGTCCG GCACGCCGCG AACCGCAAGG AACCTTCCCG ACTTAGGGGC GGAGCAGGAA GCGTCGCCGG GGGGCCCACA AGGGTAGCGG CGAAGATCCG GGTGACGCTG CGAACGGACG TGAAGAATGT GCGAGACCCA GGGTCGGCGC CGCTGCGTTT CCCGGAACCA CGCCCAGAGC AGCCGCGTCC CTGCGCAAAC CCAGGGCTGC CTTGGAAAAG GCGCAACCCC AACCCCGTGG AATTATCACC TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGAAGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGCAGACTTT ACTCCCTATC AGTGATAGAG AACGTATAAG GAGTTTACTC CCTATCAGTG ATAGAGAACG TATGACCAGT TTACTCCCTA TCAGTGATAG AGAACGTATC TACAGTTTAC TCCCTATCAG TGATAGAGAA CGTATATCCA GTTTACTCCC TATCAGTGAT AGAGAACGTA TAAGCTTTGC TTATGTAAAC CAGGGCGCCT ATAAAAGAGT GCTGATTTTT TGAGTAAACT TCAATTCCAC AACACTTTTG TCTTATACCA ACTTTCCGTA CCACTTCCTA CCCTCGTAAA GAATTCTTCC GTATACACCG GTGCCACCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGGGAG GAGGGGGAAG CATGGGCCCA GGAGTTCTGC TGCTCCTGCT GGTGGCCACA GCTTGGCATG GTCAGGGAAT CCCAGTGATA GAGCCCAGTG TCCCCGAGCT GGTCGTGAAG CCAGGAGCAA CGGTGACCTT GCGATGTGTG GGCAATGGCA GCGTGGAATG GGATGGCCCC CCATCACCTC ACTGGACCCT GTACTCTGAT GGCTCCAGCA GCATCCTCAG CACCAACAAC GCTACCTTCC AAAACACGGG GACCTATCGC TGCACTGAGC CTGGAGACCC CCTGGGAGGC AGCGCCGCCA TCCACCTCTA TGTCAAAGAC CCTGCCCGGC CCTGGAACGT GCTAGCACAG GAGGTGGTCG TGTTCGAGGA CCAGGACGCA CTACTGCCCT GTCTGCTCAC AGACCCGGTG CTGGAAGCAG GCGTCTCGCT GGTGCGTGTG CGTGGCCGGC CCCTCATGCG CCACACCAAC TACTCCTTCT CGCCCTGGCA TGGCTTCACC ATCCACAGGG CCAAGTTCAT TCAGAGCCAG GACTATCAAT GCAGTGCCCT GATGGGTGGC AGGAAGGTGA TGTCCATCAG CATCCGGCTG AAAGTGCAGA AAGTCATCCC AGGGCCCCCA GCCTTGACAC TGGTGCCTGC AGAGCTGGTG CGGATTCGAG GGGAGGCTGC CCAGATCGTG TGCTCAGCCA GCAGCGTTGA TGTTAACTTT GATGTCTTCC TCCAACACAA CAACACCAAG CTCGCAATCC ATCAACAATC TGACTTTCAT AATAACCGTT ACCAAAAAGT CCTGACCCTC AACCTCGATC AAGTAGATTT CCAACATGCC GGCAACTACT CCTGCGTGGC CAGCAACGTG CAGGGCAAGC ACTCCACCTC CATGTTCTTC CGGGTGGTAG AGAGTGCCTA CTTGAACTTG AGCTCTGAGC AGAACCTCAT CCAGGAGGTG ACCGTGGGGG AGGGGCTCAA CCTCAAAGTC ATGGTGGAGG CCTACCCAGG CCTGCAAGGT TTTAACTGGA CCTACCTGGG ACCCTTTTCT GACCACCAGC CTGAGCCCAA GCTTGCTAAT GTTACCACCA AGGACACATA CAGGCACACC TTCACCCTCT CTCTGCCCCG CCTGAAGCCC TCTGAGGCTG GCCGCTACTC CTTCCTGGCC AGAAACCCAG GAGGCTGGAG AGCTCTGACG TTTGAGCTCA CCCTTCGATA CCCCCCAGAG GTAAGCGTCA TATGGACATT CATCAACGGC TCTGGCACCC TTTTGTGTGC TGCCTCTGGG TACCCCCAGC CCAACGTGAC ATGGCTGCAG TGCAGTGGCC ACACTGATAG GTGTGATGAG GCCCAAGTGC TGCAGGTCTG GGATGACCCA TACCCTGAGG TCCTGAGCCA GGAGCCCTTC CACAAGGTGA CGGTGCAGAG CCTGCTGACT GTTGAGACCT TAGAGCACAA CCAAACCTAC GAGTGCAGGG CCCACAACAG CGTGGGGAGT GGCTCCTGGG CCTTCATACC CATCTCTGCA GGAGCCCACA CGCATCCCCC GGATGAGTTC CTCTTCACAC CAGTGGTGGT CGCCTGCATG TCCATCATGG CCTTGCTGCT GCTGCTGCTC CTGCTGCTAT TGTACAAGTA TAAGCAGAAG CCCAAGTACC AGGTCCGCTG GAAGATCATC GAGAGCTATG AGGGCAACAG TTATACTTTC ATCGACCCCA CGCAGCTGCC TTACAACGAG AAGTGGGAGT TCCCCCGGAA CAACCTGCAG TTTGGTAAGA CCCTCGGAGC TGGAGCCTTT GGGAAGGTGG TGGAGGCCAC GGCCTTTGGT CTGGGCAAGG AGGATGCTGT CCTGAAGGTG GCTGTGAAGA TGCTGAAGTC CACGGCCCAT GCTGATGAGA AGGAGTCCCT CATGTCCGAG CTGAAGATCA TGAGCCACCT GGGCCAGCAC GAGAACATCG TCAACCTTCT GGGAGCCTGT ACCCATGGAG GCCCTGTACT GGTCATCACG GAGTACTGTT GCTATGGCGA CCTGCTCAAC TTTCTGCGAA GGAAGGCTGA GGCCATGCTG GGACCCAGCC TGAGCCCCGG CCAGGACCCC GAGGGAGGCG TCGACTATAA GAACATCCAC CTCGAGAAGA AATATGTCCG CAGGGACAGT GGCTTCTCCA GCCAGGGTGT GGACACCTAT GTGGAGATGA GGCCTGTCTC CACTTCTTCA AATGACTCCT TCTCTGAGCA AGACCTGGAC AAGGAGGATG GACGGCCCCT GGAGCTCCGG GACCTGCTTC ACTTCTCCAG CCAAGTAGCC CAGGGCATGG CCTTCCTCGC TTCCAAGAAT TGCATCCACC GGGACGTGGC AGCGCGTAAC GTGCTGTTGA CCAATGGTCA TGTGGCCAAG ATTGGGGACT TCGGGCTGGC TAGGGACATC ATGAATGACT CCAACTACAT TGTCAAGGGC AATGCCCGCC TGCCTGTGAA GTGGATGGCC CCAGAGAGCA TCTTTGACTG TGTCTACACG GTTCAGAGCG ACGTCTGGTC CTATGGCATC CTCCTCTGGG AGATCTTCTC ACTTGGGCTG AATCCCTACC CTGGCATCCT GGTGAACAGC AAGTTCTATA AACTGGTGAA GGATGGATAC CAAATGGCCC AGCCTGCATT TGCCCCAAAG AATATATACA GCATCATGCA GGCCTGCTGG GCCTTGGAGC CCACCCACAG ACCCACCTTC CAGCAGATCT GCTCCTTCCT TCAGGAGCAG GCCCAAGAGG ACAGGAGAGA GCGGGACTAT ACCAATCTGC CGAGCAGCAG CAGAAGCGGT GGCAGCGGCA GCAGCAGCAG TGAGCTGGAG GAGGAGAGCT CTAGTGAGCA CCTGACCTGC TGCGAGCAAG GGGATATCGC CCAGCCCTTG CTGCAGCCCA ACAACTATCA GTTCTGCGAG CAAAAGCTCA TTTCTGAAGA GGACTTGGAT TACAAGGACG ACGATGACAA GTAGGATCCA GACCACCTCC CCTGCGAGCT AAGCTGGACA GCCAATGACG GGTAAGAGAG TGACATTTTT CACTAACCTA AGACAGGAGG GCCGTCAGAG CTACTGCCTA ATCCAAAGAC GGGTAAAAGT GATAAAAATG TATCACTCCA ACCTAAGACA GGCGCAGCTT CCGAGGGATT TGAGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCAAAAC TAGAATGCAG TGAAAAAAAT GCCTTATTTG TGAAATTTGT GATGCTATTG CCTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA GTCGATAAAC TGGATCTCTG CTGTCCCTGT AATAAACCCG AAAATTTTGA ATTTTTGTAA TTTGTTTTTG TAGTTCTTTA GTTTGTATGT CTGTTGCTAT TATGTCTACT ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAGGC GATACCGTCG AGATCCGTTC ACTAATCGAA TGGATCTGTC TCTGTCTCTC TCTCCACCTT CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG GTCTGAAACG ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA AAGTACAGCA AAAACTATTC TTAAACCTAC CAAGCCTCCT ACTATCATTA TGAATAATTT TATATACCAC AGCCAATTTG TTATGTTAAA CCAATTCCAC AAACTTGCCC ATTTATCTAA TTCCAATAAT TCTTGTTCAT TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAAGGAGTG TATTAAGCTT GTGTAATTGT TAATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGATTCCA AATCTGTTCC AGAGATTTAT TACTCCAACT AGCATTCCAA GGCACAGCAG TGGTGCAAAT GAGTTTTCCA GAGCAACCCC AAATCCCCAG GAGCTGTTGA TCCTTTAGGT ATCTTTCCAC AGCCAGGATT CTTGCCTGGA GCTGCTTGAT GCCCCAGACT GTGAGTTGCA ACAGATGCTG TTGCGCCTCA ATAGCCCTCA GCAAATTGTT CTGCTGCTGC ACTATACCAG ACAATAATTG TCTGGCCTGT ACCGTCAGCG TCATTGACGC TGCGCCCATA GTGCTTCCTG CTGCTCCCAA GAACCCAAGG AACAAAGCTC CTATTCCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTTG GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGGCC GCTTGCTGTG CGGTGGTCTT ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT TTATCTCTAT CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA GTTCTTCTGA TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT CTGATGTTTC TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA CTATATGTTT TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC GCGATCTAAT TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC CTCCGCTAGT CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC GCGCTTCAGC AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA AGTCCCTGTT CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGGC AAAAAGCAGC TGCTTATATG CAGGATCTGA GGGCTCGCCA CTCCCCAGTC CCGCCCAGGC CACGCCTCCC TGGAAAGTCC CCAGCGGAAA GTCCCTTGTA GCAAGCTCGA TATCAGCAGT TCTTGAAGTA CTCCGGATGC AGCTCTCGGG CCACGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAACACTTCT CTCTCCGGGT CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCTGGT GTTCTCTCCT TTATTGGCCT CTTCTACCTT ATCTGGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGACCCC TGGCCCTGGT GTGTAGTTCT GCTAATCAGG GAAGTAGCCT TGTGTGTGGT AGATCCACAG ATCAAGGATA TCTTGTCTTC TTTGGGAGTG AATTAGCCCT TCCA
