NM_030752.3;CCT-alpha; CCT1; CCTa; D6S230E;TCP-1-alpha
100μg liquid plasmid(Instantly):$105
| name | pADH1-GAL4AD-HA-TCP1(human)-LEU2 | another name | apADH1-GAL4AD-HA-TCP1(human)-LEU2 |
| Insert Size (bp) | 1671bp | Plasmid host | Yeast bacterium |
| Plasmid uses | Protein expression,hybridization | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | ADH1 | Replicon | pUC,2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGCATGCCTG CAGGTCGAGA TCCGGGATCG AAGAAATGAT GGTAAATGAA ATAGGAAATC AAGGAGCATG AAGGCAAAAG ACAAATATAA GGGTCGAACG AAAAATAAAG TGAAAAGTGT TGATATGATG TATTTGGCTT TGCGGCGCCG AAAAAACGAG TTTACGCAAT TGCACAATCA TGCTGACTCT GTGGCGGACC CGCGCTCTTG CCGGCCCGGC GATAACGCTG GGCGTGAGGC TGTGCCCGGC GGAGTTTTTT GCGCCTGCAT TTTCCAAGGT TTACCCTGCG CTAAGGGGCG AGATTGGAGA AGCAATAAGA ATGCCGGTTG GGGTTGCGAT GATGACGACC ACGACAACTG GTGTCATTAT TTAAGTTGCC GAAAGAACCT GAGTGCATTT GCAACATGAG TATACTAGAA GAATGAGCCA AGACTTGCGA GACGCGAGTT TGCCGGTGGT GCGAACAATA GAGCGACCAT GACCTTGAAG GTGAGACGCG CATAACCGCT AGAGTACTTT GAAGAGGAAA CAGCAATAGG GTTGCTACCA GTATAAATAG ACAGGTACAT ACAACACTGG AAATGGTTGT CTGTTTGAGT ACGCTTTCAA TTCATTTGGG TGTGCACTTT ATTATGTTAC AATATGGAAG GGAACTTTAC ACTTCTCCTA TGCACATATA TTAATTAAAG TCCAATGCTA GTAGAGAAGG GGGGTAACAC CCCTCCGCGC TCTTTTCCGA TTTTTTTCTA AACCGTGGAA TATTTCGGAT ATCCTTTTGT TGTTTCCGGG TGTACAATAT GGACTTCCTC TTTTCTGGCA ACCAAACCCA TACATCGGGA TTCCTATAAT ACCTTCGTTG GTCTCCCTAA CATGTAGGTG GCGGAGGGGA GATATACAAT AGAACAGATA CCAGACAAGA CATAATGGGC TAAACAAGAC TACACCAATT ACACTGCCTC ATTGATGGTG GTACATAACG AACTAATACT GTAGCCCTAG ACTTGATAGC CATCATCATA TCGAAGTTTC ACTACCCTTT TTCCATTTGC CATCTATTGA AGTAATAATA GGCGCATGCA ACTTCTTTTC TTTTTTTTTC TTTTCTCTCT CCCCCGTTGT TGTCTCACCA TATCCGCAAT GACAAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT CCAGTTACTT GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTTGCAAA GATGGATAAA GCGGAATTAA TTCCCGAGCC TCCAAAAAAG AAGAGAAAGG TCGAATTGGG TACCGCCGCC AATTTTAATC AAAGTGGGAA TATTGCTGAT AGCTCATTGT CCTTCACTTT CACTAACAGT AGCAACGGTC CGAACCTCAT AACAACTCAA ACAAATTCTC AAGCGCTTTC ACAACCAATT GCCTCCTCTA ACGTTCATGA TAACTTCATG AATAATGAAA TCACGGCTAG TAAAATTGAT GATGGTAATA ATTCAAAACC ACTGTCACCT GGTTGGACGG ACCAAACTGC GTATAACGCG TTTGGAATCA CTACAGGGAT GTTTAATACC ACTACAATGG ATGATGTATA TAACTATCTA TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGA TCTTTAATAC GACTCACTAT AGGGCGAGCG CCGCCATGGA GTACCCATAC GACGTACCAG ATTACGCTCA TATGGCCATG GAGGCCAGTG AATTCATGGA GGGGCCTTTG TCCGTGTTCG GTGACCGCAG CACTGGGGAA ACGATCCGCT CCCAAAACGT TATGGCTGCA GCTTCGATTG CCAATATTGT AAAAAGTTCT CTTGGTCCAG TTGGCTTGGA TAAAATGTTG GTGGATGATA TTGGTGATGT AACCATTACT AACGATGGTG CAACCATCCT GAAGTTACTG GAGGTAGAAC ATCCTGCAGC TAAAGTTCTT TGTGAGCTGG CTGATCTGCA AGACAAAGAA GTTGGAGATG GAACTACTTC AGTGGTTATT ATTGCAGCAG AACTCCTAAA AAATGCAGAT GAATTAGTCA AACAGAAAAT TCATCCCACA TCAGTTATTA GTGGCTATCG ACTTGCTTGC AAGGAAGCAG TGCGTTATAT CAATGAAAAC CTAATTGTTA ACACAGATGA ACTGGGAAGA GATTGCCTGA TTAATGCTGC TAAGACATCC ATGTCTTCCA AAATCATTGG AATAAATGGT GATTTCTTTG CTAACATGGT AGTAGATGCT GTACTTGCTA TTAAATACAC AGACATAAGA GGCCAGCCAC GCTATCCAGT CAACTCTGTT AATATTTTGA AAGCCCATGG GAGAAGTCAA ATGGAGAGTA TGCTCATCAG TGGCTATGCA CTCAACTGTG TGGTGGGATC CCAGGGCATG CCCAAGAGAA TCGTAAATGC AAAAATTGCT TGCCTTGACT TCAGCCTGCA AAAAACAAAA ATGAAGCTTG GTGTACAGGT GGTCATTACA GACCCTGAAA AACTGGACCA AATTAGACAG AGAGAATCAG ATATCACCAA GGAGAGAATT CAGAAGATCC TGGCAACTGG TGCCAATGTT ATTCTAACCA CTGGTGGAAT TGATGATATG TGTCTGAAGT ATTTTGTGGA GGCTGGTGCT ATGGCAGTTA GAAGAGTTTT AAAAAGGGAC CTTAAACGCA TTGCCAAAGC TTCTGGAGCA ACTATTCTGT CAACCCTGGC CAATTTGGAA GGTGAAGAAA CTTTTGAAGC TGCAATGTTG GGACAGGCAG AAGAAGTGGT ACAGGAGAGA ATTTGTGATG ATGAGCTGAT CTTAATCAAA AATACTAAGG CTCGTACGTC TGCATCGATT ATCTTACGTG GGGCAAATGA TTTCATGTGT GATGAGATGG AGCGCTCTTT ACATGATGCA CTTTGTGTAG TGAAGAGAGT TTTGGAGTCA AAATCTGTGG TTCCCGGTGG GGGTGCTGTA GAAGCAGCCC TTTCCATATA CCTTGAAAAC TATGCAACCA GCATGGGGTC TCGGGAACAG CTTGCGATTG CAGAGTTTGC AAGATCACTT CTTGTTATTC CCAATACACT AGCAGTTAAT GCTGCCCAGG ACTCCACAGA TCTGGTTGCA AAATTAAGAG CTTTTCATAA TGAGGCCCAG GTTAACCCAG AACGTAAAAA TCTAAAATGG ATTGGTCTTG ATTTGAGCAA TGGTAAACCT CGAGACAACA AACAAGCAGG GGTGTTTGAA CCAACCATAG TTAAAGTTAA GAGTTTGAAA TTTGCAACAG AAGCTGCAAT CACCATTCTT CGAATTGATG ATCTTATTAA ATTACATCCA GAAAGTAAAG ATGATAAACA TGGAAGTTAT GAAGATGCTG TTCACTCTGG AGCCCTTAAT GATTGAGGAT CCATCGAGCT CGAGCTGCAG ATGAATCGTA GATACTGAAA AACCCCGCAA GTTCACTTCA ACTGTGCATC GTGCACCATC TCAATTTCTT TCATTTATAC ATCGTTTTGC CTTCTTTTAT GTAACTATAC TCCTCTAAGT TTCAATCTTG GCCATGTAAC CTCTGATCTA TAGAATTTTT TAAATGACTA GAATTAATGC CCATCTTTTT TTTGGACCTA AATTCTTCAT GAAAATATAT TACGAGGGCT TATTCAGAAG CTTTGGACTT CTTCGCCAGA GGTTTGGTCA AGTCTCCAAT CAAGGTTGTC GGCTTGTCTA CCTTGCCAGA AATTTACGAA AAGATGGAAA AGGGTCAAAT CGTTGGTAGA TACGTTGTTG ACACTTCTAA ATAAGCGAAT TTCTTATGAT TTATGATTTT TATTATTAAA TAAGTTATAA AAAAAATAAG TGTATACAAA TTTTAAAGTG ACTCTTAGGT TTTAAAACGA AAATTCTTAT TCTTGAGTAA CTCTTTCCTG TAGGTCAGGT TGCTTTCTCA GGTATAGCAT GAGGTCGCTC TTATTGACCA CACCTCTACC GGCCGGTCGA AATTCCCCTA CCCTATGAAC ATATTCCATT TTGTAATTTC GTGTCGTTTC TATTATGAAT TTCATTTATA AAGTTTATGT ACAAATATCA TAAAAAAAGA GAATCTTTTT AAGCAAGGAT TTTCTTAACT TCTTCGGCGA CAGCATCACC GACTTCGGTG GTACTGTTGG AACCACCTAA ATCACCAGTT CTGATACCTG CATCCAAAAC CTTTTTAACT GCATCTTCAA TGGCCTTACC TTCTTCAGGC AAGTTCAATG ACAATTTCAA CATCATTGCA GCAGACAAGA TAGTGGCGAT AGGGTTGACC TTATTCTTTG GCAAATCTGG AGCAGAACCG TGGCATGGTT CGTACAAACC AAATGCGGTG TTCTTGTCTG GCAAAGAGGC CAAGGACGCA GATGGCAACA AACCCAAGGA ACCTGGGATA ACGGAGGCTT CATCGGAGAT GATATCACCA AACATGTTGC TGGTGATTAT AATACCATTT AGGTGGGTTG GGTTCTTAAC TAGGATCATG GCGGCAGAAT CAATCAATTG ATGTTGAACC TTCAATGTAG GAAATTCGTT CTTGATGGTT TCCTCCACAG TTTTTCTCCA TAATCTTGAA GAGGCCAAAA CATTAGCTTT ATCCAAGGAC CAAATAGGCA ATGGTGGCTC ATGTTGTAGG GCCATGAAAG CGGCCATTCT TGTGATTCTT TGCACTTCTG GAACGGTGTA TTGTTCACTA TCCCAAGCGA CACCATCACC ATCGTCTTCC TTTCTCTTAC CAAAGTAAAT ACCTCCCACT AATTCTCTGA CAACAACGAA GTCAGTACCT TTAGCAAATT GTGGCTTGAT TGGAGATAAG TCTAAAAGAG AGTCGGATGC AAAGTTACAT GGTCTTAAGT TGGCGTACAA TTGAAGTTCT TTACGGATTT TTAGTAAACC TTGTTCAGGT CTAACACTAC CTGTACCCCA TTTAGGACCA CCCACAGCAC CTAACAAAAC GGCATCAACC TTCTTGGAGG CTTCCAGCGC CTCATCTGGA AGTGGGACAC CTGTAGCGTC GATAGCAGCA CCACCAATTA AATGATTTTC GAAATCGAAC TTGACATTGG AACGAACATC AGAAATAGCT TTAAGAACCT TAATGGCTTC GGCTGTGATT TCTTGACCAA CGTGGTCACC TGGCAAAACG ACGATCTTCT TAGGGGCAGA CATTAGAATG GTATATCCTT GAAATATATA TATATATTGC TGAAATGTAA AAGGTAAGAA AAGTTAGAAA GTAAGACGAT TGCTAACCAC CTATTGGAAA AAACAATAGG TCCTTAAATA ATATTGTCAA CTTCAAGTAT TGTGATGCAA GCATTTAGTC ATGAACGCTT CTCTATTCTA TATGAAAAGC CGGTTCCGGC GCTCTCACCT TTCCTTTTTC TCCCAATTTT TCAGTTGAAA AAGGTATATG CGTCAGGCGA CCTCTGAAAT TAACAAAAAA TTTCCAGTCA TCGAATTTGA TTCTGTGCGA TAGCGCCCCT GTGTGTTCTC GTTATGTTGA GGAAAAAAAT AATGGTTGCT AAGAGATTCG AACTCTTGCA TCTTACGATA CCTGAGTATT CCCACAGTTG GGGATCTCGA CTCTAGCTAG AGGATCAATT CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGA TAACTTCGTA TAATGTATGC TATACGAAGT TATTAGGTCT GAAGAGGAGT TTACGTCCAG CCAAGCTAGC TTGGCTGCAG GTCGAGCGGC CGCGATCCGG AACCCTTAAT ATAACTTCGT ATAATGTATG CTATACGAAG TTATCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAA CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT GTATATATAT ATACAGGCAA CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT GCGCAGCTCG CGTTGCATTT TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT TCCGAAGTTC CTATTCTCTA GCTAGAAAGT ATAGGAACTT CAGAGCGCTT TTGAAAACCA AAAGCGCTCT GAAGACGCAC TTTCAAAAAA CCAAAAACGC ACCGGACTGT AACGAGCTAC TAAAATATTG CGAATACCGC TTCCACAAAC ATTGCTCAAA AGTATCTCTT TGCTATATAT CTCTGTGCTA TATCCCTATA TAACCTACCC ATCCACCTTT CGCTCCTTGA ACTTGCATCT AAACTCGACC TCTACATTTT TTATGTTTAT CTCTAGTATT ACTCTTTAGA CAAAAAAATT GTAGTAAGAA CTATTCATAG AGTGAATCGA AAACAATACG AAAATGTAAA CATTTCCTAT ACGTAGTATA TAGAGACAAA ATAGAAGAAA CCGTTCATAA TTTTCTGACC AATGAAGAAT CATCAACGCT ATCACTTTCT GTTCACAAAG TATGCGCAAT CCACATCGGT ATAGAATATA ATCGGGGATG CCTTTATCTT GAAAAAATGC ACCCGCAGCT TCGCTAGTAA TCAGTAAACG CGGGAAGTGG AGTCAGGCTT TTTTTATGGA AGAGAAAATA GACACCAAAG TAGCCTTCTT CTAACCTTAA CGGACCTACA GTGCAAAAAG TTATCAAGAG ACTGCATTAT AGAGCGCACA AAGGAGAAAA AAAGTAATCT AAGATGCTTT GTTAGAAAAA TAGCGCTCTC GGGATGCATT TTTGTAGAAC AAAAAAGAAG TATAGATTCT TTGTTGGTAA AATAGCGCTC TCGCGTTGCA TTTCTGTTCT GTAAAAATGC AGCTCAGATT CTTTGTTTGA AAAATTAGCG CTCTCGCGTT GCATTTTTGT TTTACAAAAA TGAAGCACAG ATTCTTCGTT GGTAAAATAG CGCTTTCGCG TTGCATTTCT GTTCTGTAAA AATGCAGCTC AGATTCTTTG TTTGAAAAAT TAGCGCTCTC GCGTTGCATT TTTGTTCTAC AAAATGAAGC ACAGATGCTT CGTTGCT
