pUT-mini-Tn5-Kan-Tac-mCherry-SacB
$142.00
In stock
SKU
PL51760
100μg liquid plasmid(Instantly):$185
| name | pUT-mini-Tn5-Kan-Tac-mCherry-SacB | another name | apUT-mini-Tn5-Kan-Tac-mCherry-SacB |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp,Kan | |
| Selective marker | Fluorescent labeling | Red | |
| Promoter | Tac | Replicon | R6K |
| Competence | S17-1λpir | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCCTTTTT GTCCGGTGTT GGGTTGAAGG TGAAGCCGGT CGGGGCCGCA GCGGGGGCCG GCTTTTCAGC CTTGCCCCCC TGCTTCGGCC GCCGTGGCTC CGGCGTCTTG GGTGCCGGCG CGGGTTCCGC AGCCTTGGCC TGCGGTGCGG GCACATCGGC GGGCTTGGCC TTGATGTGCC GCCTGGCGTG CGAGCGGAAC GTCTCGTAGG AGAACTTGAC CTTCCCCGTT TCCCGCATGT GCTCCCAAAT GGTGACGAGC GCATAGCCGG ACGCTAACGC CGCCTCGACA TCCGCCCTCA CCGCCAGGAA CGCAACCGCA GCCTCATCAC GCCGGCGCTT CTTGGCCGCG CGGGATTCAA CCCACTCGGC CAGCTCGTCG GTGTAGCTCT TTGGCATCGT CTCTCGCCTG TCCCCTCAGT TCAGTAATTT CCTGCATTTG CCTGTTTCCA GTCGGTAGAT ATTCCACAAA ACAGCAGGGA AGCAGCGCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GGCTACCGCC GGCGTAACAG ATGAGGGCAA GCGGATGGCT GATGAAACCA AGCCAACCAG GAAGGGCAGC CCACCTATCA AGGTGTACTG CCTTCCAGAC GAACGAAGAG CGATTGAGGA AAAGGCGGCG GCGGCCGGCA TGAGCCTGTC GGCCTACCTG CTGGCCGTCG GCCAGGGCTA CAAAATCACG GGCGTCGTGG ACTATGAGCA CGTCCGCGAG CTGGCCCGCA TCAATGGCGA CCTGGGCCGC CTGGGCGGCC TGCTGAAACT CTGGCTCACC GACGACCCGC GCACGGCGCG GTTCGGTGAT GCCACGATCC TCGCCCTGCT GGCGAAGATC GAAGAGAAGC AGGACGAGCT TGGCAAGGTC ATGATGGGCG TGGTCCGCCC GAGGGCAGAG CCATGACTTT TTTAGCCGCT AAAACGGCCG GGGGGTGCGC GTGATTGCCA AGCACGTCCC CATGCGCTCC ATCAAGAAGA GCGACTTCGC GGAGCTGGTG AAGTACATCA CCGACGAGCA AGGCAAGACC GAGCGCCTGG GTCACGTGCG CGTCACGAAC TGCGAGGCAA ACACCCTGCC CGCTGTCATG GCCGAGGTGA TGGCGACCCA GCACGGCAAC ACCCGTTCCG AGGCCGACAA GACCTATCAC CTGCTGGTTA GCTTCCGCGC GGGAGAGAAG CCCGACGCGG AGACGTTGCG CGCGATTGAG GACCGCATCT GCGCTGGGCT TGGCTTCGCC GAGCATCAGC GCGTCAGTGC CGTGCATCAC GACACCGACA ACCTGCACAT CCATATCGCC ATCAACAAGA TTCACCCGAC CCGAAACACC ATCCATGAGC CGTATCGGGC CTACCGCGCC CTCGCTGACC TCTGCGCGAC GCTCGAACGG GACTACGGGC TTGAGCGTGA CAATCACGAA ACGCGGCAGC GCGTTTCCGA GAACCGCGCG AACGACATGG AGCGGCACGC GGGCGTGGAA AGCCTGGTCG GCTGGATCCG GCCACGATGC GTCCGGCGTA GAGGATCTGA AGATCAGCAG TTCAACCTGT TGATAGTACG TACTAAGCTC TCATGTTTCA CGTACTAAGC TCTCATGTTT AACGTACTAA GCTCTCATGT TTAACGAACT AAACCCTCAT GGCTAACGTA CTAAGCTCTC ATGGCTAACG TACTAAGCTC TCATGTTTCA CGTACTAAGC TCTCATGTTT GAACAATAAA ATTAATATAA ATCAGCAACT TAAATAGCCT CTAAGGTTTT AAGTTTTATA AGAAAAAAAA GAATATATAA GGCTTTTAAA GCTTTTAAGG TTTAACGGTT GTGGACAACA AGCCAGGGAT GTAACGCACT GAGAAGCCCT TAGAGCCTCT CAAAGCAATT TTGAGTGACA CAGGAACACT TAACGGCTGA CATGGGAATT CCACATGTGG AATTCCACAT GTGGAATTGT GAGCGGATAA CAATTTGTGG AATTCCCGGG AGAGCTCCTG ACTCTTATAC ACAAGTTCGG CCACGGCCTA GGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCCAGG GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT TGCCCTTCAC CGCCTGGCCC TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG ATGGTGGTTA ACGGCGGGAT ATAACATGAG CTGTCTTCGG TATCGTCGTA TCCCACTACC GAGATATCCG CACCAACGCG CAGCCCGGAC TCGGTAATGG CGCGCATTGC GCCCAGCGCC ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC CCTCATTCAG CATTTGCATG GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC GTTCCGCTAT CGGCTGAATT TGATTGCGAG TGAGATATTT ATGCCAGCCA GCCAGACGCA GACGCGCCGA GACAGAACTT AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG CGACCAGATG CTCCACGCCC AGTCGCGTAC CGTCTTCATG GGAGAAAATA ATACTGTTGA TGGGTGTCTG GTCAGAGACA TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA CAGCAATGGC ATCCTGGTCA TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACGCGTTGCG CGAGAAGATT GTGCACCGCC GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT GGCACCCAGT TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG GCGCGTGCAG GGCCAGACTG GAGGTGGCAA CGCCAATCAG CAACGACTGT TTGCCCGCCA GTTGTTGTGC CACGCGGTTG GGAATGTAAT TCAGCTCCGC CATCGCCGCT TCCACTTTTT CCCGCGTTTT CGCAGAAACG TGGCTGGCCT GGTTCACCAC GCGGGAAACG GTCTGATAAG AGACACCGGC ATACTCTGCG ACATCGTATA ACGTTACTGG TTTCACATTC ACCACCCTGA ATTGACTCTC TTCCGGGCGC TATCATGCCA TACCGCGAAA GGTTTTGCGC CATTCGATGG TGTCCGGGAT CTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAATT TGACAATTAA TCATCGGCTC GTATAATGTG TGGAATGTCT CATGAGCGGA TATTTCACAC AGGAAACAGT ATTCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAA CGTCAACATC AAGTTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA AGTAATTAAC CTAGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGGCGG CCGCAGCTTG TCGACGAATT CCCGGGGATC CGGTGATTGA TTGAGCAAGC TTTATGCTTG TAAACCGTTT TGTGAAAAAA TTTTTAAAAT AAAAAAGGGG ACCTCTAGGG TCCCCAATTA ATTAGTAATA TAATCTATTA AAGGTCATTC AAAAGGTCAT CCACCGGATC AATTCCCCTG CTCACACAGA CTGGGTACCA AGCTCTCGGT AACATCAAGG CCCGATCCTT GGAGCCCTTG CCCTCCCGAC GATGATCGTG CCGTGATCGA AATCCAGATC CTTGACCCAC AGTTGCAAAC CCTCACTGAT CCGTCCCGTC CGGGTCGACC AAAGCGGCCA TCGTGCCTCC CCACTCCTGC AGTTCGGGGG CATGGATGCG CGGATAGCCG CTGCTGGTTT CCTGGATGCC GACGGATTTG CACTGCCGGT AGAACTCCGC GAGGTCGTCC AGCCTCAGGC AGCAGCTGAA CCAACTCGCG AGGGGATCGA GCCCGGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGGTCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCGCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCC TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCAGATCTTG ATCCCCTGCG CCATCAGATC CTTGGCGGCA AGAAAGCCAT CCAGTTTACT TTGCAGGGCT TCCCAACCTT ACCAGAGGGC GCCCCAGCTG GCAATTCCGG TTCGCTTGCT GTCCATAAAA CCGCCCAGTC TAGCTATCGT CATGTAAGCC CACTGCAAGC TACCTCTTTC TCTTTGCGCT TGCGTTTTCC CTTGTCCAGA TAGCCCAGTA GCTGACATTC ATCCGGGGTA GCACCGTTTC TGCGGACTGG CTTTCTACGT GTTCCGCTTC CTTTAGCAGC CCTTGCGCCC TGATGCTTGC GGCAGCGTGA AGCTCGCGCA GATCAGTTGG TTGATTTTGT CCACTACGTG AAAGGCGAAT CACCAAGGTA GTCGGCAAAT AATGTCTAAC AATTCGTTCA AGCCGACGCC GCTTCGCGGC GCGGTTAACT CAAGCGTTAG ATGCACTAAG CACATAATTG CTCACAGCCA AACTATCAGG TCAAGTCGCT TTTATTATTT TTAAGCGTGC ATAATAAGCC CTACACAAAA TTGGGAGATA TATCATGAAA GGCGCTTTTC TTGTTATCGC AATAGTTGGG CGAAGTAATC GCAACATCCG CATTAACAAT CTAGCGAGGG CTTGGTAAGG TGATCCGGTG GATGACCTTT TGAATGACCT TTAATAGATT ATATTACTAA TTAATTGGGG ACCCTAGAGG TCCCCTTTTT TATTTTAAAA ATTTTTTCAC AAAACGGTTT ACAAGCATAA AGCTTGCTCA ATCAATCACC GGATCCCCGG GAATTCGGCC TAGGTGGCCA GATCTGATCA AGAGACAGAA ACGCAAAAGA AAATGCCGAT ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC AATTCCCCTC TAGAAATAAT TTTGTTAACT TTAAGAAGGA GATATACATA TGATTACCAG TGCACTGCAT CGTGCGGCGG ATTGGGCGAA AAGCGTGTTT TCTAGTGCTG CGCTGGGTGA TCCGCGTCGT ACCGCGCGTC TGGTGAATGT TGCGGCGCAA CTGGCCAAAT ATAGCGGCAA AAGCATTACC ATTAGCAGCG AAGGCAGCAA AGCCATGCAG GAAGGCGCGT ATCGTTTTAT TCGTAATCCG AACGTGAGCG CGGAAGCGAT TCGTAAAGCG GGTGCCATGC AGACCGTGAA ACTGGCCCAG GAATTTCCGG AACTGCTGGC AATTGAAGAT ACCACCTCTC TGAGCTATCG TCATCAGGTG GCGGAAGAAC TGGGCAAACT GGGTAGCATT CAGGATAAAA GCCGTGGTTG GTGGGTGCAT AGCGTGCTGC TGCTGGAAGC GACCACCTTT CGTACCGTGG GCCTGCTGCA TCAAGAATGG TGGATGCGTC CGGATGATCC GGCGGATGCG GATGAAAAAG AAAGCGGCAA ATGGCTGGCC GCTGCTGCAA CTTCGCGTCT GAGAATGGGC AGCATGATGA GCAACGTGAT TGCGGTGTGC GATCGTGAAG CGGATATTCA TGCGTATCTG CAAGATAAAC TGGCCCATAA CGAACGTTTT GTGGTGCGTA GCAAACATCC GCGTAAAGAT GTGGAAAGCG GCCTGTATCT GTATGATCAC CTGAAAAACC AGCCGGAACT GGGCGGCTAT CAGATTAGCA TTCCGCAGAA AGGCGTGGTG GATAAACGTG GCAAACGTAA AAACCGTCCG GCGCGTAAAG CGAGCCTGAG CCTGCGTAGC GGCCGTATTA CCCTGAAACA GGGCAACATT ACCCTGAACG CGGTGCTGGC CGAAGAAATT AATCCGCCGA AAGGCGAAAC CCCGCTGAAA TGGCTGCTGC TGACCAGCGA GCCGGTGGAA AGTCTGGCCC AAGCGCTGCG TGTGATTGAT ATTTATACCC ATCGTTGGCG CATTGAAGAA TTTCACAAAG CGTGGAAAAC GGGTGCGGGT GCGGAACGTC AGCGTATGGA AGAACCGGAT AACCTGGAAC GTATGGTGAG CATTCTGAGC TTTGTGGCGG TGCGTCTGCT GCAACTGCGT GAATCTTTTA CTCCGCCGCA AGCACTGCGT GCGCAGGGCC TGCTGAAAGA AGCGGAACAC GTTGAAAGCC AGAGCGCGGA AACCGTGCTG ACCCCGGATG AATGCCAACT GCTGGGCTAT CTGGATAAAG GCAAACGCAA ACGCAAAGAA AAAGCGGGCA GCCTGCAATG GGCGTATATG GCGATTGCGC GTCTGGGCGG CTTTATGGAT AGCAAACGTA CCGGCATTGC GAGCTGGGGT GCGCTGTGGG AAGGTTGGGA AGCGCTGCAA AGCAAACTGG ATGGCTTTCT GGCCGCGAAA GACCTGATGG CGCAGGGCAT TAAAATCTAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGAC GCGTGCTATC GTCATGTAAG CCCACTGATC GGCATTTTCT TTTGCGTTTT TATTTGTTAA CTGTTAATTG TCCTTGTTCA AGGATGCTGT CTTTGACAAC AGATGTTTTC TTGCCTTTGA TGTTCAGCAG GAAGCTCGGC GCAAACGTTG ATTGTTTGTC TGCGTAGAAT CCTCTGTTTG TCATATAGCT TGTAATCACG ACATTGTTTC CTTTCGCTTG AGGTACAGCG AAGTGTGAGT AAGTAAAGGT TACATCGTTA GGATCAAGAT CCATTTTTAA CACAAGGCCA GTTTTGTTCA GCGGCTTGTA TGGGCCAGTT AAAGAATTAG AAACATAACC AAGCATGTAA ATATCGTTAG ACGTAATGCC GTCAATCGTC ATTTTTGATC CGCGGGAGTC AGTGAACAGG TACCATTTGC CGTTCATTTT AAAGACGTTC GCGCGTTCAA TTTCATCTGT TACTGTGTTA GATGCAATCA GCGGTTTCAT CACTTTTTTC AGTGTGTAAT CATCGTTTAG CTCAATCATA CCGAGAGCGC CGTTTGCTAA CTCAGCCGTG CGTTTTTTAT CGCTTTGCAG AAGTTTTTGA CTTTCTTGAC GGAAGAATGA TGTGCTTTTG CCATAGTATG CTTTGTTAAA TAAAGATTCT TCGCCTTGGT AGCCATCTTC AGTTCCAGTG TTTGCTTCAA ATACTAAGTA TTTGTGGCCT TTATCTTCTA CGTAGTGAGG ATCTCTCAGC GTATGGTTGT CGCCTGAGCT GTAGTTGCCT TCATCGATGA ACTGCTGTAC ATTTTGATAC GTTTTTCCGT CACCGTCAAA GATTGATTTA TAATCCTCTA CACCGTTGAT GTTCAAAGAG CTGTCTGATG CTGATACGTT AACTTGTGCA GTTGTCAGTG TTTGTTTGCC GTAATGTTTA CCGGAGAAAT CAGTGTAGAA TAAACGGATT TTTCCGTCAG ATGTAAATGT GGCTGAACCT GACCATTCTT GTGTTTGGTC TTTTAGGATA GAATCATTTG CATCGAATTT GTCGCTGTCT TTAAAGACGC GGCCAGCGTT TTTCCAGCTG TCAATAGAAG TTTCGCCGAC TTTTTGATAG AACATGTAAA TCGATGTGTC ATCCGCATTT TTAGGATCTC CGGCTAATGC AAAGACGATG TGGTAGCCGT GATAGTTTGC GACAGTGCCG TCAGCGTTTT GTAATGGCCA GCTGTCCCAA ACGTCCAGGC CTTTTGCAGA AGAGATATTT TTAATTGTGG ACGAATCAAA TTCAGAAACT TGATATTTTT CATTTTTTTG CTGTTCAGGG ATTTGCAGCA TATCATGGCG TGTAATATGG GAAATGCCGT ATGTTTCCTT ATATGGCTTT TGGTTCGTTT CTTTCGCAAA CGCTTGAGTT GCGCCTCCTG CCAGCAGTGC GGTAGTAAAG GTTAATACTG TTGCTTGTTT TGCAAACTTT TTGATGTTCA TCGTTCATGT CTCCTTTTTT ATGTACTGTG TTAGCGGTCT GCTTCTTCCA GCCCTCCTGT TTGAAGATGG CAAGTTAGTT ACGCACAATA AAAAAAGACC TAAAATATGT AAGGGGTGAC GCCAAAGTAT ACACTTTGCC CTTTACACAT TTTAGGTCTT GCCTGCTTTA TCAGTAACAA ACCCGCGCGA TTTACTTTTC GACCTCATTC TATTAGACTC TCGTTTGGAT TGCAACTGGT CTATTTTCCT CTTTTGTTTG ATAGAAAATC ATAAAAGGAT TTGCAGACTA CGGGCCTAAA GAACTAAAAA ATCTATCTGT TTCTTTTCAT TCTCTGTATT TTTTATAGTT TCTGTTGCAT GGGCATAAAG TTGCCTTTTT AATCACAATT CAGAAAATAT CATAATATCT CATTTCACTA AATAATAGTG AACGGCAGGT ATATGTGGGT TACCGACTGC GGCCTGAGTT TTTTAAGTGA CGTAAAATCG TGTTGAGGCC AACGCCCATA ATGCGGGCTG TTGCCCGGCA TCCAACGCCA TTCATGGCCA TATCAATGAT TTTCTGGTGC GTACCGGGTT GAGAAGCGGT GTAAGTGAAC TGCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCA
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