pADH1-GAL4AD-HA-CCT2(human)-LEU2
$84.00
In stock
SKU
PL51722
NM_006431.3;99D8.1; CCT-beta; CCTB; HEL-S-100n
100μg liquid plasmid(Instantly):$105
| name | pADH1-GAL4AD-HA-CCT2(human)-LEU2 | another name | apADH1-GAL4AD-HA-CCT2(human)-LEU2 |
| Insert Size (bp) | 1608bp | Plasmid host | Yeast bacterium |
| Plasmid uses | Protein expression,hybridization | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | ADH1 | Replicon | pUC,2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGCATGCCTG CAGGTCGAGA TCCGGGATCG AAGAAATGAT GGTAAATGAA ATAGGAAATC AAGGAGCATG AAGGCAAAAG ACAAATATAA GGGTCGAACG AAAAATAAAG TGAAAAGTGT TGATATGATG TATTTGGCTT TGCGGCGCCG AAAAAACGAG TTTACGCAAT TGCACAATCA TGCTGACTCT GTGGCGGACC CGCGCTCTTG CCGGCCCGGC GATAACGCTG GGCGTGAGGC TGTGCCCGGC GGAGTTTTTT GCGCCTGCAT TTTCCAAGGT TTACCCTGCG CTAAGGGGCG AGATTGGAGA AGCAATAAGA ATGCCGGTTG GGGTTGCGAT GATGACGACC ACGACAACTG GTGTCATTAT TTAAGTTGCC GAAAGAACCT GAGTGCATTT GCAACATGAG TATACTAGAA GAATGAGCCA AGACTTGCGA GACGCGAGTT TGCCGGTGGT GCGAACAATA GAGCGACCAT GACCTTGAAG GTGAGACGCG CATAACCGCT AGAGTACTTT GAAGAGGAAA CAGCAATAGG GTTGCTACCA GTATAAATAG ACAGGTACAT ACAACACTGG AAATGGTTGT CTGTTTGAGT ACGCTTTCAA TTCATTTGGG TGTGCACTTT ATTATGTTAC AATATGGAAG GGAACTTTAC ACTTCTCCTA TGCACATATA TTAATTAAAG TCCAATGCTA GTAGAGAAGG GGGGTAACAC CCCTCCGCGC TCTTTTCCGA TTTTTTTCTA AACCGTGGAA TATTTCGGAT ATCCTTTTGT TGTTTCCGGG TGTACAATAT GGACTTCCTC TTTTCTGGCA ACCAAACCCA TACATCGGGA TTCCTATAAT ACCTTCGTTG GTCTCCCTAA CATGTAGGTG GCGGAGGGGA GATATACAAT AGAACAGATA CCAGACAAGA CATAATGGGC TAAACAAGAC TACACCAATT ACACTGCCTC ATTGATGGTG GTACATAACG AACTAATACT GTAGCCCTAG ACTTGATAGC CATCATCATA TCGAAGTTTC ACTACCCTTT TTCCATTTGC CATCTATTGA AGTAATAATA GGCGCATGCA ACTTCTTTTC TTTTTTTTTC TTTTCTCTCT CCCCCGTTGT TGTCTCACCA TATCCGCAAT GACAAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT CCAGTTACTT GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTTGCAAA GATGGATAAA GCGGAATTAA TTCCCGAGCC TCCAAAAAAG AAGAGAAAGG TCGAATTGGG TACCGCCGCC AATTTTAATC AAAGTGGGAA TATTGCTGAT AGCTCATTGT CCTTCACTTT CACTAACAGT AGCAACGGTC CGAACCTCAT AACAACTCAA ACAAATTCTC AAGCGCTTTC ACAACCAATT GCCTCCTCTA ACGTTCATGA TAACTTCATG AATAATGAAA TCACGGCTAG TAAAATTGAT GATGGTAATA ATTCAAAACC ACTGTCACCT GGTTGGACGG ACCAAACTGC GTATAACGCG TTTGGAATCA CTACAGGGAT GTTTAATACC ACTACAATGG ATGATGTATA TAACTATCTA TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGA TCTTTAATAC GACTCACTAT AGGGCGAGCG CCGCCATGGA GTACCCATAC GACGTACCAG ATTACGCTCA TATGGCCATG GAGGCCAGTG AATTCATGGC GTCCCTTTCC CTTGCACCTG TTAACATCTT TAAGGCAGGA GCTGATGAAG AGAGAGCAGA GACAGCTCGT CTGACTTCTT TTATTGGTGC CATCGCCATT GGAGACTTGG TAAAGAGCAC CTTGGGACCC AAAGGCATGG ACAAAATTCT TCTAAGCAGT GGACGAGATG CCTCTCTTAT GGTAACCAAT GATGGTGCCA CTATTCTAAA AAACATTGGT GTTGACAATC CAGCAGCTAA AGTTTTAGTT GATATGTCAA GGGTTCAAGA TGATGAAGTT GGTGATGGCA CTACCTCTGT TACCGTTTTA GCAGCAGAAT TATTAAGGGA AGCAGAATCT TTAATTGCAA AAAAGATTCA TCCACAGACC ATCATAGCGG GTTGGAGAGA AGCCACGAAG GCTGCAAGAG AGGCGCTGTT GAGTTCTGCA GTTGATCATG GTTCCGATGA AGTTAAATTC CGTCAAGATT TAATGAATAT TGCGGGCACA ACATTATCCT CAAAACTTCT TACTCATCAC AAAGACCACT TTACAAAGTT AGCTGTAGAA GCAGTTCTCA GACTGAAAGG CTCTGGCAAC CTGGAGGCAA TTCATATTAT CAAGAAGCTA GGAGGAAGTT TGGCAGATTC CTATTTAGAT GAAGGCTTCC TGTTGGATAA AAAAATTGGA GTAAATCAAC CAAAACGAAT TGAAAATGCT AAAATTCTTA TTGCAAATAC TGGTATGGAT ACAGACAAAA TAAAGATATT TGGTTCCCGG GTAAGAGTTG ACTCTACAGC AAAGGTTGCA GAAATAGAAC ATGCGGAAAA GGAAAAAATG AAGGAGAAAG TTGAACGTAT TCTTAAGCAT GGAATAAATT GCTTTATTAA CAGGCAATTA ATTTATAATT ATCCTGAACA GCTCTTTGGT GCTGCTGGTG TCATGGCTAT TGAGCATGCA GATTTTGCAG GTGTGGAACG CCTAGCTCTT GTCACAGGTG GTGAAATTGC CTCTACCTTT GATCACCCAG AACTGGTGAA GCTTGGAAGT TGCAAACTTA TCGAGGAAGT CATGATTGGA GAAGACAAAC TCATTCACTT TTCTGGGGTT GCCCTTGGTG AGGCTTGTAC CATTGTTTTG CGTGGTGCCA CTCAACAAAT TTTAGATGAA GCAGAAAGAT CATTGCATGA TGCTCTTTGT GTTCTTGCGC AAACTGTAAA GGACTCTAGA ACAGTTTATG GAGGAGGCTG TTCTGAGATG TTGATGGCTC ATGCTGTGAC ACAGCTTGCC AATAGAACAC CAGGCAAAGA AGCTGTTGCA ATGGAGTCTT ATGCTAAAGC ACTGAGAATG TTGCCAACCA TCATAGCTGA CAATGCAGGC TATGACAGTG CAGACCTGGT GGCACAGCTC AGGGCTGCTC ACAGTGAAGG CAATACCACT GCTGGATTGG ATATGAGGGA AGGCACCATT GGAGATATGG CTATCCTGGG TATAACAGAA AGTTTTCAAG TGAAGCGACA GGTTCTTCTG AGTGCAGCTG AAGCAGCAGA GGTGATTCTG CGTGTGGACA ACATCATCAA AGCGGCACCC AGGAAACGTG TCCCTGATCA CCACCCCTGT TAAGGATCCA TCGAGCTCGA GCTGCAGATG AATCGTAGAT ACTGAAAAAC CCCGCAAGTT CACTTCAACT GTGCATCGTG CACCATCTCA ATTTCTTTCA TTTATACATC GTTTTGCCTT CTTTTATGTA ACTATACTCC TCTAAGTTTC AATCTTGGCC ATGTAACCTC TGATCTATAG AATTTTTTAA ATGACTAGAA TTAATGCCCA TCTTTTTTTT GGACCTAAAT TCTTCATGAA AATATATTAC GAGGGCTTAT TCAGAAGCTT TGGACTTCTT CGCCAGAGGT TTGGTCAAGT CTCCAATCAA GGTTGTCGGC TTGTCTACCT TGCCAGAAAT TTACGAAAAG ATGGAAAAGG GTCAAATCGT TGGTAGATAC GTTGTTGACA CTTCTAAATA AGCGAATTTC TTATGATTTA TGATTTTTAT TATTAAATAA GTTATAAAAA AAATAAGTGT ATACAAATTT TAAAGTGACT CTTAGGTTTT AAAACGAAAA TTCTTATTCT TGAGTAACTC TTTCCTGTAG GTCAGGTTGC TTTCTCAGGT ATAGCATGAG GTCGCTCTTA TTGACCACAC CTCTACCGGC CGGTCGAAAT TCCCCTACCC TATGAACATA TTCCATTTTG TAATTTCGTG TCGTTTCTAT TATGAATTTC ATTTATAAAG TTTATGTACA AATATCATAA AAAAAGAGAA TCTTTTTAAG CAAGGATTTT CTTAACTTCT TCGGCGACAG CATCACCGAC TTCGGTGGTA CTGTTGGAAC CACCTAAATC ACCAGTTCTG ATACCTGCAT CCAAAACCTT TTTAACTGCA TCTTCAATGG CCTTACCTTC TTCAGGCAAG TTCAATGACA ATTTCAACAT CATTGCAGCA GACAAGATAG TGGCGATAGG GTTGACCTTA TTCTTTGGCA AATCTGGAGC AGAACCGTGG CATGGTTCGT ACAAACCAAA TGCGGTGTTC TTGTCTGGCA AAGAGGCCAA GGACGCAGAT GGCAACAAAC CCAAGGAACC TGGGATAACG GAGGCTTCAT CGGAGATGAT ATCACCAAAC ATGTTGCTGG TGATTATAAT ACCATTTAGG TGGGTTGGGT TCTTAACTAG GATCATGGCG GCAGAATCAA TCAATTGATG TTGAACCTTC AATGTAGGAA ATTCGTTCTT GATGGTTTCC TCCACAGTTT TTCTCCATAA TCTTGAAGAG GCCAAAACAT TAGCTTTATC CAAGGACCAA ATAGGCAATG GTGGCTCATG TTGTAGGGCC ATGAAAGCGG CCATTCTTGT GATTCTTTGC ACTTCTGGAA CGGTGTATTG TTCACTATCC CAAGCGACAC CATCACCATC GTCTTCCTTT CTCTTACCAA AGTAAATACC TCCCACTAAT TCTCTGACAA CAACGAAGTC AGTACCTTTA GCAAATTGTG GCTTGATTGG AGATAAGTCT AAAAGAGAGT CGGATGCAAA GTTACATGGT CTTAAGTTGG CGTACAATTG AAGTTCTTTA CGGATTTTTA GTAAACCTTG TTCAGGTCTA ACACTACCTG TACCCCATTT AGGACCACCC ACAGCACCTA ACAAAACGGC ATCAACCTTC TTGGAGGCTT CCAGCGCCTC ATCTGGAAGT GGGACACCTG TAGCGTCGAT AGCAGCACCA CCAATTAAAT GATTTTCGAA ATCGAACTTG ACATTGGAAC GAACATCAGA AATAGCTTTA AGAACCTTAA TGGCTTCGGC TGTGATTTCT TGACCAACGT GGTCACCTGG CAAAACGACG ATCTTCTTAG GGGCAGACAT TAGAATGGTA TATCCTTGAA ATATATATAT ATATTGCTGA AATGTAAAAG GTAAGAAAAG TTAGAAAGTA AGACGATTGC TAACCACCTA TTGGAAAAAA CAATAGGTCC TTAAATAATA TTGTCAACTT CAAGTATTGT GATGCAAGCA TTTAGTCATG AACGCTTCTC TATTCTATAT GAAAAGCCGG TTCCGGCGCT CTCACCTTTC CTTTTTCTCC CAATTTTTCA GTTGAAAAAG GTATATGCGT CAGGCGACCT CTGAAATTAA CAAAAAATTT CCAGTCATCG AATTTGATTC TGTGCGATAG CGCCCCTGTG TGTTCTCGTT ATGTTGAGGA AAAAAATAAT GGTTGCTAAG AGATTCGAAC TCTTGCATCT TACGATACCT GAGTATTCCC ACAGTTGGGG ATCTCGACTC TAGCTAGAGG ATCAATTCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGATAA CTTCGTATAA TGTATGCTAT ACGAAGTTAT TAGGTCTGAA GAGGAGTTTA CGTCCAGCCA AGCTAGCTTG GCTGCAGGTC GAGCGGCCGC GATCCGGAAC CCTTAATATA ACTTCGTATA ATGTATGCTA TACGAAGTTA TCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAACGC ATTTAAGCAT AAACACGCAC TATGCCGTTC TTCTCATGTA TATATATATA CAGGCAACAC GCAGATATAG GTGCGACGTG AACAGTGAGC TGTATGTGCG CAGCTCGCGT TGCATTTTCG GAAGCGCTCG TTTTCGGAAA CGCTTTGAAG TTCCTATTCC GAAGTTCCTA TTCTCTAGCT AGAAAGTATA GGAACTTCAG AGCGCTTTTG AAAACCAAAA GCGCTCTGAA GACGCACTTT CAAAAAACCA AAAACGCACC GGACTGTAAC GAGCTACTAA AATATTGCGA ATACCGCTTC CACAAACATT GCTCAAAAGT ATCTCTTTGC TATATATCTC TGTGCTATAT CCCTATATAA CCTACCCATC CACCTTTCGC TCCTTGAACT TGCATCTAAA CTCGACCTCT ACATTTTTTA TGTTTATCTC TAGTATTACT CTTTAGACAA AAAAATTGTA GTAAGAACTA TTCATAGAGT GAATCGAAAA CAATACGAAA ATGTAAACAT TTCCTATACG TAGTATATAG AGACAAAATA GAAGAAACCG TTCATAATTT TCTGACCAAT GAAGAATCAT CAACGCTATC ACTTTCTGTT CACAAAGTAT GCGCAATCCA CATCGGTATA GAATATAATC GGGGATGCCT TTATCTTGAA AAAATGCACC CGCAGCTTCG CTAGTAATCA GTAAACGCGG GAAGTGGAGT CAGGCTTTTT TTATGGAAGA GAAAATAGAC ACCAAAGTAG CCTTCTTCTA ACCTTAACGG ACCTACAGTG CAAAAAGTTA TCAAGAGACT GCATTATAGA GCGCACAAAG GAGAAAAAAA GTAATCTAAG ATGCTTTGTT AGAAAAATAG CGCTCTCGGG ATGCATTTTT GTAGAACAAA AAAGAAGTAT AGATTCTTTG TTGGTAAAAT AGCGCTCTCG CGTTGCATTT CTGTTCTGTA AAAATGCAGC TCAGATTCTT TGTTTGAAAA ATTAGCGCTC TCGCGTTGCA TTTTTGTTTT ACAAAAATGA AGCACAGATT CTTCGTTGGT AAAATAGCGC TTTCGCGTTG CATTTCTGTT CTGTAAAAAT GCAGCTCAGA TTCTTTGTTT GAAAAATTAG CGCTCTCGCG TTGCATTTTT GTTCTACAAA ATGAAGCACA GATGCTTCGT TGCT
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