pADH1-GAL4AD-HA-CCT7(human)-LEU2
$84.00
In stock
SKU
PL51589
NM_006429.4;CCTETA; CCTH; NIP7-1; TCP1ETA
100μg liquid plasmid(Instantly):$105
| name | pADH1-GAL4AD-HA-CCT7(human)-LEU2 | another name | apADH1-GAL4AD-HA-CCT7(human)-LEU2 |
| Insert Size (bp) | 1632bp | Plasmid host | Yeast bacterium |
| Plasmid uses | Protein expression,hybridization | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | ADH1 | Replicon | pUC,2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGCATGCCTG CAGGTCGAGA TCCGGGATCG AAGAAATGAT GGTAAATGAA ATAGGAAATC AAGGAGCATG AAGGCAAAAG ACAAATATAA GGGTCGAACG AAAAATAAAG TGAAAAGTGT TGATATGATG TATTTGGCTT TGCGGCGCCG AAAAAACGAG TTTACGCAAT TGCACAATCA TGCTGACTCT GTGGCGGACC CGCGCTCTTG CCGGCCCGGC GATAACGCTG GGCGTGAGGC TGTGCCCGGC GGAGTTTTTT GCGCCTGCAT TTTCCAAGGT TTACCCTGCG CTAAGGGGCG AGATTGGAGA AGCAATAAGA ATGCCGGTTG GGGTTGCGAT GATGACGACC ACGACAACTG GTGTCATTAT TTAAGTTGCC GAAAGAACCT GAGTGCATTT GCAACATGAG TATACTAGAA GAATGAGCCA AGACTTGCGA GACGCGAGTT TGCCGGTGGT GCGAACAATA GAGCGACCAT GACCTTGAAG GTGAGACGCG CATAACCGCT AGAGTACTTT GAAGAGGAAA CAGCAATAGG GTTGCTACCA GTATAAATAG ACAGGTACAT ACAACACTGG AAATGGTTGT CTGTTTGAGT ACGCTTTCAA TTCATTTGGG TGTGCACTTT ATTATGTTAC AATATGGAAG GGAACTTTAC ACTTCTCCTA TGCACATATA TTAATTAAAG TCCAATGCTA GTAGAGAAGG GGGGTAACAC CCCTCCGCGC TCTTTTCCGA TTTTTTTCTA AACCGTGGAA TATTTCGGAT ATCCTTTTGT TGTTTCCGGG TGTACAATAT GGACTTCCTC TTTTCTGGCA ACCAAACCCA TACATCGGGA TTCCTATAAT ACCTTCGTTG GTCTCCCTAA CATGTAGGTG GCGGAGGGGA GATATACAAT AGAACAGATA CCAGACAAGA CATAATGGGC TAAACAAGAC TACACCAATT ACACTGCCTC ATTGATGGTG GTACATAACG AACTAATACT GTAGCCCTAG ACTTGATAGC CATCATCATA TCGAAGTTTC ACTACCCTTT TTCCATTTGC CATCTATTGA AGTAATAATA GGCGCATGCA ACTTCTTTTC TTTTTTTTTC TTTTCTCTCT CCCCCGTTGT TGTCTCACCA TATCCGCAAT GACAAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT CCAGTTACTT GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTTGCAAA GATGGATAAA GCGGAATTAA TTCCCGAGCC TCCAAAAAAG AAGAGAAAGG TCGAATTGGG TACCGCCGCC AATTTTAATC AAAGTGGGAA TATTGCTGAT AGCTCATTGT CCTTCACTTT CACTAACAGT AGCAACGGTC CGAACCTCAT AACAACTCAA ACAAATTCTC AAGCGCTTTC ACAACCAATT GCCTCCTCTA ACGTTCATGA TAACTTCATG AATAATGAAA TCACGGCTAG TAAAATTGAT GATGGTAATA ATTCAAAACC ACTGTCACCT GGTTGGACGG ACCAAACTGC GTATAACGCG TTTGGAATCA CTACAGGGAT GTTTAATACC ACTACAATGG ATGATGTATA TAACTATCTA TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGA TCTTTAATAC GACTCACTAT AGGGCGAGCG CCGCCATGGA GTACCCATAC GACGTACCAG ATTACGCTCA TATGGCCATG GAGGCCAGTG AATTCATGAT GCCCACACCA GTTATCCTAT TGAAAGAGGG GACTGATAGC TCCCAAGGCA TCCCCCAGCT TGTGAGTAAC ATCAGTGCCT GCCAGGTGAT TGCTGAGGCT GTAAGAACTA CCCTGGGTCC CCGTGGCATG GACAAGCTTA TTGTAGATGG CAGAGGCAAA GCAACAATTT CTAATGATGG GGCCACAATT CTGAAACTTC TTGATGTTGT CCATCCTGCA GCAAAGACTT TGGTAGACAT TGCCAAATCC CAAGATGCTG AGGTGGGTGA TGGCACCACC TCAGTGACCT TGCTGGCTGC AGAGTTTCTG AAGCAGGTGA AACCCTATGT GGAGGAAGGT TTACACCCCC AGATCATCAT TCGAGCTTTC CGCACAGCCA CCCAGCTGGC AGTTAACAAG ATCAAAGAGA TTGCTGTGAC CGTGAAGAAG GCAGATAAAG TGGAGCAGAG GAAGCTGCTG GAAAAGTGTG CCATGACCGC TCTGAGCTCC AAGCTGATCT CCCAGCAGAA AGCTTTCTTT GCTAAGATGG TGGTGGATGC AGTGATGATG CTCGATGATT TGCTGCAGCT TAAAATGATT GGAATCAAGA AGGTACAGGG TGGAGCCCTC GAGGATTCTC AGCTGGTAGC TGGTGTTGCA TTCAAGAAGA CTTTCTCTTA CGCTGGGTTT GAAATGCAAC CCAAAAAGTA CCACAATCCC AAGATTGCCC TTTTGAATGT CGAGCTCGAG TTGAAAGCTG AGAAAGACAA TGCTGAGATA AGAGTCCACA CAGTTGAGGA TTATCAGGCA ATTGTTGATG CTGAGTGGAA CATTCTCTAT GACAAGTTAG AGAAGATCCA TCATTCTGGA GCCAAAGTTG TCTTGTCCAA ACTCCCCATT GGGGATGTGG CCACCCAGTA CTTTGCTGAC AGGGACATGT TCTGTGCTGG CCGAGTACCT GAGGAGGATC TGAAGAGGAC AATGATGGCC TGTGGAGGCT CAATCCAGAC CAGTGTGAAT GCTCTGTCAG CAGATGTGCT GGGTCGATGC CAGGTGTTTG AAGAGACCCA GATTGGAGGC GAGAGGTACA ATTTTTTTAC TGGCTGCCCC AAGGCCAAGA CATGCACCTT CATTCTCCGT GGCGGCGCCG AGCAGTTTAT GGAGGAGACA GAGCGGTCCC TGCATGATGC CATCATGATC GTCAGGAGGG CCATCAAGAA TGATTCAGTG GTGGCTGGTG GCGGGGCCAT TGAGATGGAA CTCTCCAAGT ACCTGCGGGA TTACTCAAGG ACTATTCCAG GAAAACAGCA GCTGTTGATT GGGGCATATG CCAAGGCCTT GGAGATTATC CCACGCCAGC TGTGTGACAA TGCTGGCTTT GATGCCACAA ACATTCTCAA CAAGCTGCGG GCTCGGCATG CCCAGGGGGG TACATGGTAT GGAGTAGACA TCAACAACGA GGACATTGCT GACAACTTTG AAGCTTTCGT GTGGGAGCCA GCTATGGTGC GGATCAATGC GCTGACAGCA GCCTCTGAGG CTGCGTGCCT GATCGTGTCT GTAGATGAAA CCATCAAGAA CCCCCGCTCG ACTGTGGATG CTCCCACAGC AGCAGGCCGG GGCCGTGGTC GTGGCCGCCC CCACTGAGGA TCCATCGAGC TCGAGCTGCA GATGAATCGT AGATACTGAA AAACCCCGCA AGTTCACTTC AACTGTGCAT CGTGCACCAT CTCAATTTCT TTCATTTATA CATCGTTTTG CCTTCTTTTA TGTAACTATA CTCCTCTAAG TTTCAATCTT GGCCATGTAA CCTCTGATCT ATAGAATTTT TTAAATGACT AGAATTAATG CCCATCTTTT TTTTGGACCT AAATTCTTCA TGAAAATATA TTACGAGGGC TTATTCAGAA GCTTTGGACT TCTTCGCCAG AGGTTTGGTC AAGTCTCCAA TCAAGGTTGT CGGCTTGTCT ACCTTGCCAG AAATTTACGA AAAGATGGAA AAGGGTCAAA TCGTTGGTAG ATACGTTGTT GACACTTCTA AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA AAAAAAATAA GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA TTCTTGAGTA ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGCA TGAGGTCGCT CTTATTGACC ACACCTCTAC CGGCCGGTCG AAATTCCCCT ACCCTATGAA CATATTCCAT TTTGTAATTT CGTGTCGTTT CTATTATGAA TTTCATTTAT AAAGTTTATG TACAAATATC ATAAAAAAAG AGAATCTTTT TAAGCAAGGA TTTTCTTAAC TTCTTCGGCG ACAGCATCAC CGACTTCGGT GGTACTGTTG GAACCACCTA AATCACCAGT TCTGATACCT GCATCCAAAA CCTTTTTAAC TGCATCTTCA ATGGCCTTAC CTTCTTCAGG CAAGTTCAAT GACAATTTCA ACATCATTGC AGCAGACAAG ATAGTGGCGA TAGGGTTGAC CTTATTCTTT GGCAAATCTG GAGCAGAACC GTGGCATGGT TCGTACAAAC CAAATGCGGT GTTCTTGTCT GGCAAAGAGG CCAAGGACGC AGATGGCAAC AAACCCAAGG AACCTGGGAT AACGGAGGCT TCATCGGAGA TGATATCACC AAACATGTTG CTGGTGATTA TAATACCATT TAGGTGGGTT GGGTTCTTAA CTAGGATCAT GGCGGCAGAA TCAATCAATT GATGTTGAAC CTTCAATGTA GGAAATTCGT TCTTGATGGT TTCCTCCACA GTTTTTCTCC ATAATCTTGA AGAGGCCAAA ACATTAGCTT TATCCAAGGA CCAAATAGGC AATGGTGGCT CATGTTGTAG GGCCATGAAA GCGGCCATTC TTGTGATTCT TTGCACTTCT GGAACGGTGT ATTGTTCACT ATCCCAAGCG ACACCATCAC CATCGTCTTC CTTTCTCTTA CCAAAGTAAA TACCTCCCAC TAATTCTCTG ACAACAACGA AGTCAGTACC TTTAGCAAAT TGTGGCTTGA TTGGAGATAA GTCTAAAAGA GAGTCGGATG CAAAGTTACA TGGTCTTAAG TTGGCGTACA ATTGAAGTTC TTTACGGATT TTTAGTAAAC CTTGTTCAGG TCTAACACTA CCTGTACCCC ATTTAGGACC ACCCACAGCA CCTAACAAAA CGGCATCAAC CTTCTTGGAG GCTTCCAGCG CCTCATCTGG AAGTGGGACA CCTGTAGCGT CGATAGCAGC ACCACCAATT AAATGATTTT CGAAATCGAA CTTGACATTG GAACGAACAT CAGAAATAGC TTTAAGAACC TTAATGGCTT CGGCTGTGAT TTCTTGACCA ACGTGGTCAC CTGGCAAAAC GACGATCTTC TTAGGGGCAG ACATTAGAAT GGTATATCCT TGAAATATAT ATATATATTG CTGAAATGTA AAAGGTAAGA AAAGTTAGAA AGTAAGACGA TTGCTAACCA CCTATTGGAA AAAACAATAG GTCCTTAAAT AATATTGTCA ACTTCAAGTA TTGTGATGCA AGCATTTAGT CATGAACGCT TCTCTATTCT ATATGAAAAG CCGGTTCCGG CGCTCTCACC TTTCCTTTTT CTCCCAATTT TTCAGTTGAA AAAGGTATAT GCGTCAGGCG ACCTCTGAAA TTAACAAAAA ATTTCCAGTC ATCGAATTTG ATTCTGTGCG ATAGCGCCCC TGTGTGTTCT CGTTATGTTG AGGAAAAAAA TAATGGTTGC TAAGAGATTC GAACTCTTGC ATCTTACGAT ACCTGAGTAT TCCCACAGTT GGGGATCTCG ACTCTAGCTA GAGGATCAAT TCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG ATAACTTCGT ATAATGTATG CTATACGAAG TTATTAGGTC TGAAGAGGAG TTTACGTCCA GCCAAGCTAG CTTGGCTGCA GGTCGAGCGG CCGCGATCCG GAACCCTTAA TATAACTTCG TATAATGTAT GCTATACGAA GTTATCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA ACGCATTTAA GCATAAACAC GCACTATGCC GTTCTTCTCA TGTATATATA TATACAGGCA ACACGCAGAT ATAGGTGCGA CGTGAACAGT GAGCTGTATG TGCGCAGCTC GCGTTGCATT TTCGGAAGCG CTCGTTTTCG GAAACGCTTT GAAGTTCCTA TTCCGAAGTT CCTATTCTCT AGCTAGAAAG TATAGGAACT TCAGAGCGCT TTTGAAAACC AAAAGCGCTC TGAAGACGCA CTTTCAAAAA ACCAAAAACG CACCGGACTG TAACGAGCTA CTAAAATATT GCGAATACCG CTTCCACAAA CATTGCTCAA AAGTATCTCT TTGCTATATA TCTCTGTGCT ATATCCCTAT ATAACCTACC CATCCACCTT TCGCTCCTTG AACTTGCATC TAAACTCGAC CTCTACATTT TTTATGTTTA TCTCTAGTAT TACTCTTTAG ACAAAAAAAT TGTAGTAAGA ACTATTCATA GAGTGAATCG AAAACAATAC GAAAATGTAA ACATTTCCTA TACGTAGTAT ATAGAGACAA AATAGAAGAA ACCGTTCATA ATTTTCTGAC CAATGAAGAA TCATCAACGC TATCACTTTC TGTTCACAAA GTATGCGCAA TCCACATCGG TATAGAATAT AATCGGGGAT GCCTTTATCT TGAAAAAATG CACCCGCAGC TTCGCTAGTA ATCAGTAAAC GCGGGAAGTG GAGTCAGGCT TTTTTTATGG AAGAGAAAAT AGACACCAAA GTAGCCTTCT TCTAACCTTA ACGGACCTAC AGTGCAAAAA GTTATCAAGA GACTGCATTA TAGAGCGCAC AAAGGAGAAA AAAAGTAATC TAAGATGCTT TGTTAGAAAA ATAGCGCTCT CGGGATGCAT TTTTGTAGAA CAAAAAAGAA GTATAGATTC TTTGTTGGTA AAATAGCGCT CTCGCGTTGC ATTTCTGTTC TGTAAAAATG CAGCTCAGAT TCTTTGTTTG AAAAATTAGC GCTCTCGCGT TGCATTTTTG TTTTACAAAA ATGAAGCACA GATTCTTCGT TGGTAAAATA GCGCTTTCGC GTTGCATTTC TGTTCTGTAA AAATGCAGCT CAGATTCTTT GTTTGAAAAA TTAGCGCTCT CGCGTTGCAT TTTTGTTCTA CAAAATGAAG CACAGATGCT TCGTTGCT
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