NM_012073.5;CCT-epsilon; CCTE; HEL-S-69; PNAS-102;TCP-1-epsilon
100μg liquid plasmid(Instantly):$105
| name | pADH1-GAL4AD-HA-CCT5(human)-LEU2 | another name | apADH1-GAL4AD-HA-CCT5(human)-LEU2 |
| Insert Size (bp) | 1626bp | Plasmid host | Yeast bacterium |
| Plasmid uses | Protein expression,hybridization | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | ADH1 | Replicon | pUC,2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGCATGCCTG CAGGTCGAGA TCCGGGATCG AAGAAATGAT GGTAAATGAA ATAGGAAATC AAGGAGCATG AAGGCAAAAG ACAAATATAA GGGTCGAACG AAAAATAAAG TGAAAAGTGT TGATATGATG TATTTGGCTT TGCGGCGCCG AAAAAACGAG TTTACGCAAT TGCACAATCA TGCTGACTCT GTGGCGGACC CGCGCTCTTG CCGGCCCGGC GATAACGCTG GGCGTGAGGC TGTGCCCGGC GGAGTTTTTT GCGCCTGCAT TTTCCAAGGT TTACCCTGCG CTAAGGGGCG AGATTGGAGA AGCAATAAGA ATGCCGGTTG GGGTTGCGAT GATGACGACC ACGACAACTG GTGTCATTAT TTAAGTTGCC GAAAGAACCT GAGTGCATTT GCAACATGAG TATACTAGAA GAATGAGCCA AGACTTGCGA GACGCGAGTT TGCCGGTGGT GCGAACAATA GAGCGACCAT GACCTTGAAG GTGAGACGCG CATAACCGCT AGAGTACTTT GAAGAGGAAA CAGCAATAGG GTTGCTACCA GTATAAATAG ACAGGTACAT ACAACACTGG AAATGGTTGT CTGTTTGAGT ACGCTTTCAA TTCATTTGGG TGTGCACTTT ATTATGTTAC AATATGGAAG GGAACTTTAC ACTTCTCCTA TGCACATATA TTAATTAAAG TCCAATGCTA GTAGAGAAGG GGGGTAACAC CCCTCCGCGC TCTTTTCCGA TTTTTTTCTA AACCGTGGAA TATTTCGGAT ATCCTTTTGT TGTTTCCGGG TGTACAATAT GGACTTCCTC TTTTCTGGCA ACCAAACCCA TACATCGGGA TTCCTATAAT ACCTTCGTTG GTCTCCCTAA CATGTAGGTG GCGGAGGGGA GATATACAAT AGAACAGATA CCAGACAAGA CATAATGGGC TAAACAAGAC TACACCAATT ACACTGCCTC ATTGATGGTG GTACATAACG AACTAATACT GTAGCCCTAG ACTTGATAGC CATCATCATA TCGAAGTTTC ACTACCCTTT TTCCATTTGC CATCTATTGA AGTAATAATA GGCGCATGCA ACTTCTTTTC TTTTTTTTTC TTTTCTCTCT CCCCCGTTGT TGTCTCACCA TATCCGCAAT GACAAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT CCAGTTACTT GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTTGCAAA GATGGATAAA GCGGAATTAA TTCCCGAGCC TCCAAAAAAG AAGAGAAAGG TCGAATTGGG TACCGCCGCC AATTTTAATC AAAGTGGGAA TATTGCTGAT AGCTCATTGT CCTTCACTTT CACTAACAGT AGCAACGGTC CGAACCTCAT AACAACTCAA ACAAATTCTC AAGCGCTTTC ACAACCAATT GCCTCCTCTA ACGTTCATGA TAACTTCATG AATAATGAAA TCACGGCTAG TAAAATTGAT GATGGTAATA ATTCAAAACC ACTGTCACCT GGTTGGACGG ACCAAACTGC GTATAACGCG TTTGGAATCA CTACAGGGAT GTTTAATACC ACTACAATGG ATGATGTATA TAACTATCTA TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGA TCTTTAATAC GACTCACTAT AGGGCGAGCG CCGCCATGGA GTACCCATAC GACGTACCAG ATTACGCTCA TATGGCCATG GAGGCCAGTG AATTCATGGC GTCCATGGGG ACCCTCGCCT TCGATGAATA TGGGCGCCCT TTCCTCATCA TCAAGGATCA GGACCGCAAG TCCCGTCTTA TGGGACTTGA GGCCCTCAAG TCTCATATAA TGGCAGCAAA GGCTGTAGCA AATACAATGA GAACATCACT TGGACCAAAT GGGCTTGATA AGATGATGGT GGATAAGGAT GGAGATGTGA CTGTAACTAA TGATGGGGCC ACCATCTTAA GCATGATGGA TGTTGATCAT CAGATTGCCA AGCTGATGGT GGAACTGTCC AAGTCTCAGG ATGATGAAAT TGGAGATGGA ACCACAGGAG TGGTTGTCCT GGCTGGTGCC TTGTTAGAAG AAGCGGAGCA ATTGCTAGAC CGAGGCATTC ACCCAATCAG AATAGCCGAT GGCTATGAGC AGGCTGCTCG TGTTGCTATT GAACACCTGG ACAAGATCAG CGATAGCGTC CTTGTTGACA TAAAGGACAC CGAACCCCTG ATTCAGACAG CAAAAACCAC GCTGGGCTCC AAAGTGGTCA ACAGTTGTCA CCGACAGATG GCTGAGATTG CTGTGAATGC CGTCCTCACT GTAGCAGATA TGGAGCGGAG AGACGTTGAC TTTGAGCTTA TCAAAGTAGA AGGCAAAGTG GGCGGCAGGC TGGAGGACAC TAAACTGATT AAGGGCGTGA TTGTGGACAA GGATTTCAGT CACCCACAGA TGCCAAAAAA AGTGGAAGAT GCGAAGATTG CAATTCTCAC ATGTCCATTT GAACCACCCA AACCAAAAAC AAAGCATAAG CTGGATGTGA CCTCTGTCGA AGATTATAAA GCCCTTCAGA AATACGAAAA GGAGAAATTT GAAGAGATGA TTCAACAAAT TAAAGAGACT GGTGCTAACC TAGCAATTTG TCAGTGGGGC TTTGATGATG AAGCAAATCA CTTACTTCTT CAGAACAACT TGCCTGCGGT TCGCTGGGTA GGAGGACCTG AAATTGAGCT GATTGCCATC GCAACAGGAG GGCGGATCGT CCCCAGGTTC TCAGAGCTCA CAGCCGAGAA GCTGGGCTTT GCTGGTCTTG TACAGGAGAT CTCATTTGGG ACAACTAAGG ATAAAATGCT GGTCATCGAG CAGTGTAAGA ACTCCAGAGC TGTAACCATT TTTATTAGAG GAGGAAATAA GATGATCATT GAGGAGGCGA AACGATCCCT TCACGATGCT TTGTGTGTCA TCCGGAACCT CATCCGCGAT AATCGTGTGG TGTATGGAGG AGGGGCTGCT GAGATATCCT GTGCCCTGGC AGTTAGCCAA GAGGCGGATA AGTGCCCCAC CTTAGAACAG TATGCCATGA GAGCGTTTGC CGACGCACTG GAGGTCATCC CCATGGCCCT CTCTGAAAAC AGTGGCATGA ATCCCATCCA GACTATGACC GAAGTCCGAG CCAGACAGGT GAAGGAGATG AACCCTGCTC TTGGCATCGA CTGTTTGCAC AAGGGGACAA ATGATATGAA GCAACAGCAT GTCATAGAAA CCTTGATTGG CAAAAAGCAA CAGATATCTC TTGCAACACA AATGGTTAGA ATGATTTTGA AGATTGATGA CATTCGTAAG CCTGGAGAAT CTGAAGAATG AGGATCCATC GAGCTCGAGC TGCAGATGAA TCGTAGATAC TGAAAAACCC CGCAAGTTCA CTTCAACTGT GCATCGTGCA CCATCTCAAT TTCTTTCATT TATACATCGT TTTGCCTTCT TTTATGTAAC TATACTCCTC TAAGTTTCAA TCTTGGCCAT GTAACCTCTG ATCTATAGAA TTTTTTAAAT GACTAGAATT AATGCCCATC TTTTTTTTGG ACCTAAATTC TTCATGAAAA TATATTACGA GGGCTTATTC AGAAGCTTTG GACTTCTTCG CCAGAGGTTT GGTCAAGTCT CCAATCAAGG TTGTCGGCTT GTCTACCTTG CCAGAAATTT ACGAAAAGAT GGAAAAGGGT CAAATCGTTG GTAGATACGT TGTTGACACT TCTAAATAAG CGAATTTCTT ATGATTTATG ATTTTTATTA TTAAATAAGT TATAAAAAAA ATAAGTGTAT ACAAATTTTA AAGTGACTCT TAGGTTTTAA AACGAAAATT CTTATTCTTG AGTAACTCTT TCCTGTAGGT CAGGTTGCTT TCTCAGGTAT AGCATGAGGT CGCTCTTATT GACCACACCT CTACCGGCCG GTCGAAATTC CCCTACCCTA TGAACATATT CCATTTTGTA ATTTCGTGTC GTTTCTATTA TGAATTTCAT TTATAAAGTT TATGTACAAA TATCATAAAA AAAGAGAATC TTTTTAAGCA AGGATTTTCT TAACTTCTTC GGCGACAGCA TCACCGACTT CGGTGGTACT GTTGGAACCA CCTAAATCAC CAGTTCTGAT ACCTGCATCC AAAACCTTTT TAACTGCATC TTCAATGGCC TTACCTTCTT CAGGCAAGTT CAATGACAAT TTCAACATCA TTGCAGCAGA CAAGATAGTG GCGATAGGGT TGACCTTATT CTTTGGCAAA TCTGGAGCAG AACCGTGGCA TGGTTCGTAC AAACCAAATG CGGTGTTCTT GTCTGGCAAA GAGGCCAAGG ACGCAGATGG CAACAAACCC AAGGAACCTG GGATAACGGA GGCTTCATCG GAGATGATAT CACCAAACAT GTTGCTGGTG ATTATAATAC CATTTAGGTG GGTTGGGTTC TTAACTAGGA TCATGGCGGC AGAATCAATC AATTGATGTT GAACCTTCAA TGTAGGAAAT TCGTTCTTGA TGGTTTCCTC CACAGTTTTT CTCCATAATC TTGAAGAGGC CAAAACATTA GCTTTATCCA AGGACCAAAT AGGCAATGGT GGCTCATGTT GTAGGGCCAT GAAAGCGGCC ATTCTTGTGA TTCTTTGCAC TTCTGGAACG GTGTATTGTT CACTATCCCA AGCGACACCA TCACCATCGT CTTCCTTTCT CTTACCAAAG TAAATACCTC CCACTAATTC TCTGACAACA ACGAAGTCAG TACCTTTAGC AAATTGTGGC TTGATTGGAG ATAAGTCTAA AAGAGAGTCG GATGCAAAGT TACATGGTCT TAAGTTGGCG TACAATTGAA GTTCTTTACG GATTTTTAGT AAACCTTGTT CAGGTCTAAC ACTACCTGTA CCCCATTTAG GACCACCCAC AGCACCTAAC AAAACGGCAT CAACCTTCTT GGAGGCTTCC AGCGCCTCAT CTGGAAGTGG GACACCTGTA GCGTCGATAG CAGCACCACC AATTAAATGA TTTTCGAAAT CGAACTTGAC ATTGGAACGA ACATCAGAAA TAGCTTTAAG AACCTTAATG GCTTCGGCTG TGATTTCTTG ACCAACGTGG TCACCTGGCA AAACGACGAT CTTCTTAGGG GCAGACATTA GAATGGTATA TCCTTGAAAT ATATATATAT ATTGCTGAAA TGTAAAAGGT AAGAAAAGTT AGAAAGTAAG ACGATTGCTA ACCACCTATT GGAAAAAACA ATAGGTCCTT AAATAATATT GTCAACTTCA AGTATTGTGA TGCAAGCATT TAGTCATGAA CGCTTCTCTA TTCTATATGA AAAGCCGGTT CCGGCGCTCT CACCTTTCCT TTTTCTCCCA ATTTTTCAGT TGAAAAAGGT ATATGCGTCA GGCGACCTCT GAAATTAACA AAAAATTTCC AGTCATCGAA TTTGATTCTG TGCGATAGCG CCCCTGTGTG TTCTCGTTAT GTTGAGGAAA AAAATAATGG TTGCTAAGAG ATTCGAACTC TTGCATCTTA CGATACCTGA GTATTCCCAC AGTTGGGGAT CTCGACTCTA GCTAGAGGAT CAATTCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGATAACT TCGTATAATG TATGCTATAC GAAGTTATTA GGTCTGAAGA GGAGTTTACG TCCAGCCAAG CTAGCTTGGC TGCAGGTCGA GCGGCCGCGA TCCGGAACCC TTAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTATC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATAACGCAT TTAAGCATAA ACACGCACTA TGCCGTTCTT CTCATGTATA TATATATACA GGCAACACGC AGATATAGGT GCGACGTGAA CAGTGAGCTG TATGTGCGCA GCTCGCGTTG CATTTTCGGA AGCGCTCGTT TTCGGAAACG CTTTGAAGTT CCTATTCCGA AGTTCCTATT CTCTAGCTAG AAAGTATAGG AACTTCAGAG CGCTTTTGAA AACCAAAAGC GCTCTGAAGA CGCACTTTCA AAAAACCAAA AACGCACCGG ACTGTAACGA GCTACTAAAA TATTGCGAAT ACCGCTTCCA CAAACATTGC TCAAAAGTAT CTCTTTGCTA TATATCTCTG TGCTATATCC CTATATAACC TACCCATCCA CCTTTCGCTC CTTGAACTTG CATCTAAACT CGACCTCTAC ATTTTTTATG TTTATCTCTA GTATTACTCT TTAGACAAAA AAATTGTAGT AAGAACTATT CATAGAGTGA ATCGAAAACA ATACGAAAAT GTAAACATTT CCTATACGTA GTATATAGAG ACAAAATAGA AGAAACCGTT CATAATTTTC TGACCAATGA AGAATCATCA ACGCTATCAC TTTCTGTTCA CAAAGTATGC GCAATCCACA TCGGTATAGA ATATAATCGG GGATGCCTTT ATCTTGAAAA AATGCACCCG CAGCTTCGCT AGTAATCAGT AAACGCGGGA AGTGGAGTCA GGCTTTTTTT ATGGAAGAGA AAATAGACAC CAAAGTAGCC TTCTTCTAAC CTTAACGGAC CTACAGTGCA AAAAGTTATC AAGAGACTGC ATTATAGAGC GCACAAAGGA GAAAAAAAGT AATCTAAGAT GCTTTGTTAG AAAAATAGCG CTCTCGGGAT GCATTTTTGT AGAACAAAAA AGAAGTATAG ATTCTTTGTT GGTAAAATAG CGCTCTCGCG TTGCATTTCT GTTCTGTAAA AATGCAGCTC AGATTCTTTG TTTGAAAAAT TAGCGCTCTC GCGTTGCATT TTTGTTTTAC AAAAATGAAG CACAGATTCT TCGTTGGTAA AATAGCGCTT TCGCGTTGCA TTTCTGTTCT GTAAAAATGC AGCTCAGATT CTTTGTTTGA AAAATTAGCG CTCTCGCGTT GCATTTTTGT TCTACAAAAT GAAGCACAGA TGCTTCGTTG CT
