pADH1-GAL4AD-HA-CCT4(human)-1-LEU2
$84.00
In stock
SKU
PL51270
NM_006430.4;CCT-DELTA; Cctd; SRB
100μg liquid plasmid(Instantly):$105
| name | pADH1-GAL4AD-HA-CCT4(human)-1-LEU2 | another name | apADH1-GAL4AD-HA-CCT4(human)-1-LEU2 |
| Insert Size (bp) | 1620bp | Plasmid host | Yeast bacterium |
| Plasmid uses | Protein expression,hybridization | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | ADH1 | Replicon | pUC,2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGCATGCCTG CAGGTCGAGA TCCGGGATCG AAGAAATGAT GGTAAATGAA ATAGGAAATC AAGGAGCATG AAGGCAAAAG ACAAATATAA GGGTCGAACG AAAAATAAAG TGAAAAGTGT TGATATGATG TATTTGGCTT TGCGGCGCCG AAAAAACGAG TTTACGCAAT TGCACAATCA TGCTGACTCT GTGGCGGACC CGCGCTCTTG CCGGCCCGGC GATAACGCTG GGCGTGAGGC TGTGCCCGGC GGAGTTTTTT GCGCCTGCAT TTTCCAAGGT TTACCCTGCG CTAAGGGGCG AGATTGGAGA AGCAATAAGA ATGCCGGTTG GGGTTGCGAT GATGACGACC ACGACAACTG GTGTCATTAT TTAAGTTGCC GAAAGAACCT GAGTGCATTT GCAACATGAG TATACTAGAA GAATGAGCCA AGACTTGCGA GACGCGAGTT TGCCGGTGGT GCGAACAATA GAGCGACCAT GACCTTGAAG GTGAGACGCG CATAACCGCT AGAGTACTTT GAAGAGGAAA CAGCAATAGG GTTGCTACCA GTATAAATAG ACAGGTACAT ACAACACTGG AAATGGTTGT CTGTTTGAGT ACGCTTTCAA TTCATTTGGG TGTGCACTTT ATTATGTTAC AATATGGAAG GGAACTTTAC ACTTCTCCTA TGCACATATA TTAATTAAAG TCCAATGCTA GTAGAGAAGG GGGGTAACAC CCCTCCGCGC TCTTTTCCGA TTTTTTTCTA AACCGTGGAA TATTTCGGAT ATCCTTTTGT TGTTTCCGGG TGTACAATAT GGACTTCCTC TTTTCTGGCA ACCAAACCCA TACATCGGGA TTCCTATAAT ACCTTCGTTG GTCTCCCTAA CATGTAGGTG GCGGAGGGGA GATATACAAT AGAACAGATA CCAGACAAGA CATAATGGGC TAAACAAGAC TACACCAATT ACACTGCCTC ATTGATGGTG GTACATAACG AACTAATACT GTAGCCCTAG ACTTGATAGC CATCATCATA TCGAAGTTTC ACTACCCTTT TTCCATTTGC CATCTATTGA AGTAATAATA GGCGCATGCA ACTTCTTTTC TTTTTTTTTC TTTTCTCTCT CCCCCGTTGT TGTCTCACCA TATCCGCAAT GACAAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT CCAGTTACTT GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTTGCAAA GATGGATAAA GCGGAATTAA TTCCCGAGCC TCCAAAAAAG AAGAGAAAGG TCGAATTGGG TACCGCCGCC AATTTTAATC AAAGTGGGAA TATTGCTGAT AGCTCATTGT CCTTCACTTT CACTAACAGT AGCAACGGTC CGAACCTCAT AACAACTCAA ACAAATTCTC AAGCGCTTTC ACAACCAATT GCCTCCTCTA ACGTTCATGA TAACTTCATG AATAATGAAA TCACGGCTAG TAAAATTGAT GATGGTAATA ATTCAAAACC ACTGTCACCT GGTTGGACGG ACCAAACTGC GTATAACGCG TTTGGAATCA CTACAGGGAT GTTTAATACC ACTACAATGG ATGATGTATA TAACTATCTA TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGA TCTTTAATAC GACTCACTAT AGGGCGAGCG CCGCCATGGA GTACCCATAC GACGTACCAG ATTACGCTCA TATGGCCATG GAGGCCAGTG AATTCATGCC CGAGAATGTG GCACCCCGGA GCGGGGCGAC TGCCGGGGCT GCCGGCGGCC GCGGGAAAGG CGCCTATCAG GACCGCGACA AGCCAGCCCA GATCCGCTTC AGCAACATTT CCGCCGCCAA AGCGGTTGCT GATGCTATTA GAACAAGCCT TGGACCAAAA GGAATGGATA AAATGATTCA AGATGGAAAA GGTGATGTAA CCATTACAAA TGATGGTGCT ACCATTCTGA AACAAATGCA AGTATTACAT CCAGCAGCCA GAATGCTGGT GGAGCTGTCT AAGGCTCAAG ATATAGAAGC AGGAGATGGC ACCACATCAG TAGTCATCAT TGCTGGCTCC CTCTTAGATT CTTGTACCAA GCTTCTTCAG AAAGGGATTC ATCCAACCAT CATTTCTGAG TCATTCCAGA AGGCCCTGGA AAAGGGCATT GAAATCTTGA CTGACATGTC TCGACCTGTG GAACTGAGTG ACAGAGAAAC TTTGTTAAAT AGTGCAACCA CTTCACTGAA CTCAAAGGTG GTTTCTCAGT ATTCAAGTCT GCTTTCTCCA ATGAGTGTAA ATGCAGTGAT GAAAGTGATT GACCCAGCCA CAGCCACCAG TGTAGATCTT AGAGATATTA AAATAGTTAA GAAGCTTGGT GGGACAATTG ATGACTGTGA GTTGGTGGAA GGGCTGGTTC TCACCCAAAA AGTGTCAAAT TCTGGCATAA CCAGAGTTGA AAAGGCCAAG ATTGGGCTTA TTCAGTTTTG CTTATCTGCT CCCAAAACAG ACATGGATAA TCAAATAGTG GTTTCTGACT ATGCCCAGAT GGACCGAGTG CTGCGAGAAG AGAGAGCCTA TATTTTAAAT TTAGTGAAGC AAATTAAAAA AACAGGATGT AATGTCCTTC TCATACAGAA ATCTATTCTA AGAGATGCTC TTAGTGATCT TGCATTACAC TTTCTGAATA AAATGAAGAT CATGGTGATT AAGGATATTG AAAGAGAAGA CATTGAATTC ATTTGTAAGA CAATTGGAAC CAAGCCAGTT GCTCATATTG ACCAATTTAC TGCTGACATG CTGGGTTCTG CTGAGTTAGC TGAGGAGGTC AATTTAAATG GTTCTGGCAA ACTGCTCAAG ATTACAGGCT GTGCCAGCCC TGGAAAAACA GTTACAATTG TTGTTCGTGG TTCTAACAAA CTGGTGATTG AAGAAGCTGA GCGCTCCATT CATGATGCCC TATGTGTTAT TCGTTGTTTA GTGAAGAAGA GGGCTCTTAT TGCAGGAGGT GGTGCTCCAG AAATAGAGTT GGCCCTACGA TTAACTGAAT ATTCACGAAC ACTGAGTGGT ATGGAATCCT ACTGCGTTCG TGCTTTTGCA GATGCTATGG AGGTCATTCC ATCTACACTA GCTGAAAATG CCGGCCTGAA TCCCATTTCT ACAGTAACAG AACTAAGAAA CCGGCATGCC CAGGGAGAAA AAACTGCAGG CATTAATGTC CGAAAGGGTG GTATTTCCAA CATTTTGGAG GAACTGGTTG TCCAGCCTCT GTTGGTATCA GTCAGTGCTC TGACTCTTGC AACTGAAACT GTTCGGAGCA TTCTGAAAAT AGATGATGTG GTAAACACTC GATAAGGATC CATCGAGCTC GAGCTGCAGA TGAATCGTAG ATACTGAAAA ACCCCGCAAG TTCACTTCAA CTGTGCATCG TGCACCATCT CAATTTCTTT CATTTATACA TCGTTTTGCC TTCTTTTATG TAACTATACT CCTCTAAGTT TCAATCTTGG CCATGTAACC TCTGATCTAT AGAATTTTTT AAATGACTAG AATTAATGCC CATCTTTTTT TTGGACCTAA ATTCTTCATG AAAATATATT ACGAGGGCTT ATTCAGAAGC TTTGGACTTC TTCGCCAGAG GTTTGGTCAA GTCTCCAATC AAGGTTGTCG GCTTGTCTAC CTTGCCAGAA ATTTACGAAA AGATGGAAAA GGGTCAAATC GTTGGTAGAT ACGTTGTTGA CACTTCTAAA TAAGCGAATT TCTTATGATT TATGATTTTT ATTATTAAAT AAGTTATAAA AAAAATAAGT GTATACAAAT TTTAAAGTGA CTCTTAGGTT TTAAAACGAA AATTCTTATT CTTGAGTAAC TCTTTCCTGT AGGTCAGGTT GCTTTCTCAG GTATAGCATG AGGTCGCTCT TATTGACCAC ACCTCTACCG GCCGGTCGAA ATTCCCCTAC CCTATGAACA TATTCCATTT TGTAATTTCG TGTCGTTTCT ATTATGAATT TCATTTATAA AGTTTATGTA CAAATATCAT AAAAAAAGAG AATCTTTTTA AGCAAGGATT TTCTTAACTT CTTCGGCGAC AGCATCACCG ACTTCGGTGG TACTGTTGGA ACCACCTAAA TCACCAGTTC TGATACCTGC ATCCAAAACC TTTTTAACTG CATCTTCAAT GGCCTTACCT TCTTCAGGCA AGTTCAATGA CAATTTCAAC ATCATTGCAG CAGACAAGAT AGTGGCGATA GGGTTGACCT TATTCTTTGG CAAATCTGGA GCAGAACCGT GGCATGGTTC GTACAAACCA AATGCGGTGT TCTTGTCTGG CAAAGAGGCC AAGGACGCAG ATGGCAACAA ACCCAAGGAA CCTGGGATAA CGGAGGCTTC ATCGGAGATG ATATCACCAA ACATGTTGCT GGTGATTATA ATACCATTTA GGTGGGTTGG GTTCTTAACT AGGATCATGG CGGCAGAATC AATCAATTGA TGTTGAACCT TCAATGTAGG AAATTCGTTC TTGATGGTTT CCTCCACAGT TTTTCTCCAT AATCTTGAAG AGGCCAAAAC ATTAGCTTTA TCCAAGGACC AAATAGGCAA TGGTGGCTCA TGTTGTAGGG CCATGAAAGC GGCCATTCTT GTGATTCTTT GCACTTCTGG AACGGTGTAT TGTTCACTAT CCCAAGCGAC ACCATCACCA TCGTCTTCCT TTCTCTTACC AAAGTAAATA CCTCCCACTA ATTCTCTGAC AACAACGAAG TCAGTACCTT TAGCAAATTG TGGCTTGATT GGAGATAAGT CTAAAAGAGA GTCGGATGCA AAGTTACATG GTCTTAAGTT GGCGTACAAT TGAAGTTCTT TACGGATTTT TAGTAAACCT TGTTCAGGTC TAACACTACC TGTACCCCAT TTAGGACCAC CCACAGCACC TAACAAAACG GCATCAACCT TCTTGGAGGC TTCCAGCGCC TCATCTGGAA GTGGGACACC TGTAGCGTCG ATAGCAGCAC CACCAATTAA ATGATTTTCG AAATCGAACT TGACATTGGA ACGAACATCA GAAATAGCTT TAAGAACCTT AATGGCTTCG GCTGTGATTT CTTGACCAAC GTGGTCACCT GGCAAAACGA CGATCTTCTT AGGGGCAGAC ATTAGAATGG TATATCCTTG AAATATATAT ATATATTGCT GAAATGTAAA AGGTAAGAAA AGTTAGAAAG TAAGACGATT GCTAACCACC TATTGGAAAA AACAATAGGT CCTTAAATAA TATTGTCAAC TTCAAGTATT GTGATGCAAG CATTTAGTCA TGAACGCTTC TCTATTCTAT ATGAAAAGCC GGTTCCGGCG CTCTCACCTT TCCTTTTTCT CCCAATTTTT CAGTTGAAAA AGGTATATGC GTCAGGCGAC CTCTGAAATT AACAAAAAAT TTCCAGTCAT CGAATTTGAT TCTGTGCGAT AGCGCCCCTG TGTGTTCTCG TTATGTTGAG GAAAAAAATA ATGGTTGCTA AGAGATTCGA ACTCTTGCAT CTTACGATAC CTGAGTATTC CCACAGTTGG GGATCTCGAC TCTAGCTAGA GGATCAATTC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGAT AACTTCGTAT AATGTATGCT ATACGAAGTT ATTAGGTCTG AAGAGGAGTT TACGTCCAGC CAAGCTAGCT TGGCTGCAGG TCGAGCGGCC GCGATCCGGA ACCCTTAATA TAACTTCGTA TAATGTATGC TATACGAAGT TATCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATAAC GCATTTAAGC ATAAACACGC ACTATGCCGT TCTTCTCATG TATATATATA TACAGGCAAC ACGCAGATAT AGGTGCGACG TGAACAGTGA GCTGTATGTG CGCAGCTCGC GTTGCATTTT CGGAAGCGCT CGTTTTCGGA AACGCTTTGA AGTTCCTATT CCGAAGTTCC TATTCTCTAG CTAGAAAGTA TAGGAACTTC AGAGCGCTTT TGAAAACCAA AAGCGCTCTG AAGACGCACT TTCAAAAAAC CAAAAACGCA CCGGACTGTA ACGAGCTACT AAAATATTGC GAATACCGCT TCCACAAACA TTGCTCAAAA GTATCTCTTT GCTATATATC TCTGTGCTAT ATCCCTATAT AACCTACCCA TCCACCTTTC GCTCCTTGAA CTTGCATCTA AACTCGACCT CTACATTTTT TATGTTTATC TCTAGTATTA CTCTTTAGAC AAAAAAATTG TAGTAAGAAC TATTCATAGA GTGAATCGAA AACAATACGA AAATGTAAAC ATTTCCTATA CGTAGTATAT AGAGACAAAA TAGAAGAAAC CGTTCATAAT TTTCTGACCA ATGAAGAATC ATCAACGCTA TCACTTTCTG TTCACAAAGT ATGCGCAATC CACATCGGTA TAGAATATAA TCGGGGATGC CTTTATCTTG AAAAAATGCA CCCGCAGCTT CGCTAGTAAT CAGTAAACGC GGGAAGTGGA GTCAGGCTTT TTTTATGGAA GAGAAAATAG ACACCAAAGT AGCCTTCTTC TAACCTTAAC GGACCTACAG TGCAAAAAGT TATCAAGAGA CTGCATTATA GAGCGCACAA AGGAGAAAAA AAGTAATCTA AGATGCTTTG TTAGAAAAAT AGCGCTCTCG GGATGCATTT TTGTAGAACA AAAAAGAAGT ATAGATTCTT TGTTGGTAAA ATAGCGCTCT CGCGTTGCAT TTCTGTTCTG TAAAAATGCA GCTCAGATTC TTTGTTTGAA AAATTAGCGC TCTCGCGTTG CATTTTTGTT TTACAAAAAT GAAGCACAGA TTCTTCGTTG GTAAAATAGC GCTTTCGCGT TGCATTTCTG TTCTGTAAAA ATGCAGCTCA GATTCTTTGT TTGAAAAATT AGCGCTCTCG CGTTGCATTT TTGTTCTACA AAATGAAGCA CAGATGCTTC GTTGCT
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