NM_201414.3;AAA; ABETA; ABPP; AD1; alpha-sAPP; APPI;CTFgamma; CVAP; PN-II; PN2; preA4
100μg liquid plasmid(Instantly):$120
| name | pTol2-appb-APPsw-CMV-EGFP | another name | apTol2-appb-APPsw-CMV-EGFP |
| Insert Size (bp) | 2088bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | APPB | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA CCAAGGTAGC TTATTGTAAA GCGTACTAAT AAGTATTAGA CAAATATAAT AAAAAATAAA AGAATAATGT CATGCTGACC TTTGCCTTAT ATATCCTCTT GCAGAAGCTC CTCGCGCAAC CGATCGCTCT CAGGGAAGTG AAATGAATGG ATCACACATT CGTGTAGTTT CTCATTTAAG ACGCGCCTAC GCATCATCCT ACTCACACAC GCCGCAGAGC CACGGGGGTG TGACTGACTT CTCTGTTTTA TTGATGTTGT CAGTTACCCA AATGTGACCT ATAATCCTAC AGGTGACTGG TGCACATGAC TGTCTAACTT GTTTACTATT TTGAGAAGCA AGTTATAGCA AGGTGAGACA AAATCTCAGG TTTTGCTGGA ATTTACTTCT GTTTTTGTCC ATGAGATGGC GCTAGAGGTG TTTTCCATAA CACTTTTACT TGTGGCTGAT TTTTCGTATT TAAATGTTGT TGTGGAAAGT TTATTGACAT ATTGGCTGTA TTGCAGGTGA AGTGTAATAC TCGTTTGTAT TCAAAGGTGT AGCTTATAAA TGCTGATGGA GAATGCAGAA TTATTGTTAT GGTTTACTAT TCAGGGTGTC CGCGAGGTCC TAAATTTCAA AAACTAAATG TTAGACCTTA AAAAGTCTTA GATACACTTA AGTATTTTGT TGTAGGTCCT AAGTCATGTT AAAGAGGTCT TAATTTTGTC CAAGTAAAGC TACCCAATCA CCTACTCAAA GTTGTAAAAC CTCAGATTTA ACTTCAGTAG AAGCTGATTC TCAAAATTGT TAGTGTCAAG CCTAGCTCTT TTGGGGCTGA AAAGCAATCC TGCAGTGCTG AAAAGCCTCT CACAGGCAGC CGATGCGGGA AGAGGTGTAT TAGTCTTGAT AGAGAGGCTG CAAATAGCAG GAAACGTGAG CAGAGACTCC CTGGTGTCTG AAACACAGGC CAGATATGCT GCCCGGTTTG GCACTGCTCC TGCTGGCCGC CTGGACGGCT CGGGCGCTGG AGGTACCCAC TGATGGTAAT GCTGGCCTGC TGGCTGAACC CCAGATTGCC ATGTTCTGTG GCAGACTGAA CATGCACATG AATGTCCAGA ATGGGAAGTG GGATTCAGAT CCATCAGGGA CCAAAACCTG CATTGATACC AAGGAAGGCA TCCTGCAGTA TTGCCAAGAA GTCTACCCTG AACTGCAGAT CACCAATGTG GTAGAAGCCA ACCAACCAGT GACCATCCAG AACTGGTGCA AGCGGGGCCG CAAGCAGTGC AAGACCCATC CCCACTTTGT GATTCCCTAC CGCTGCTTAG TTGGTGAGTT TGTAAGTGAT GCCCTTCTCG TTCCTGACAA GTGCAAATTC TTACACCAGG AGAGGATGGA TGTTTGCGAA ACTCATCTTC ACTGGCACAC CGTCGCCAAA GAGACATGCA GTGAGAAGAG TACCAACTTG CATGACTACG GCATGTTGCT GCCCTGCGGA ATTGACAAGT TCCGAGGGGT AGAGTTTGTG TGTTGCCCAC TGGCTGAAGA AAGTGACAAT GTGGATTCTG CTGATGCGGA GGAGGATGAC TCGGATGTCT GGTGGGGCGG AGCAGACACA GACTATGCAG ATGGGAGTGA AGACAAAGTA GTAGAAGTAG CAGAGGAGGA AGAAGTGGCT GAGGTGGAAG AAGAAGAAGC CGATGATGAC GAGGACGATG AGGATGGTGA TGAGGTAGAG GAAGAGGCTG AGGAACCCTA CGAAGAAGCC ACAGAGAGAA CCACCAGCAT TGCCACCACC ACCACCACCA CCACAGAGTC TGTGGAAGAG GTGGTTCGAG TTCCTACAAC AGCAGCCAGT ACCCCTGATG CCGTTGACAA GTATCTCGAG ACACCTGGGG ATGAGAATGA ACATGCCCAT TTCCAGAAAG CCAAAGAGAG GCTTGAGGCC AAGCACCGAG AGAGAATGTC CCAGGTCATG AGAGAATGGG AAGAGGCAGA ACGTCAAGCA AAGAACTTGC CTAAAGCTGA TAAGAAGGCA GTTATCCAGC ATTTCCAGGA GAAAGTGGAA TCTTTGGAAC AGGAAGCAGC CAACGAGAGA CAGCAGCTGG TGGAGACACA CATGGCCAGA GTGGAAGCCA TGCTCAATGA CCGCCGCCGC CTGGCCCTGG AGAACTACAT CACCGCTCTG CAGGCTGTTC CTCCTCGGCC TCGTCACGTG TTCAATATGC TAAAGAAGTA TGTCCGCGCA GAACAGAAGG ACAGACAGCA CACCCTAAAG CATTTCGAGC ATGTGCGCAT GGTGGATCCC AAGAAAGCCG CTCAGATCCG GTCCCAGGTT ATGACACACC TCCGTGTGAT TTATGAGCGC ATGAATCAGT CTCTCTCCCT GCTCTACAAC GTGCCTGCAG TGGCCGAGGA GATTCAGGAT GAAGTTGATG AGCTGCTTCA GAAAGAGCAA AACTATTCAG ATGACGTCTT GGCCAACATG ATTAGTGAAC CAAGGATCAG TTACGGAAAC GATGCTCTCA TGCCATCTTT GACCGAAACG AAAACCACCG TGGAGCTCCT TCCCGTGAAT GGAGAGTTCA GCCTGGACGA TCTCCAGCCG TGGCATTCTT TTGGGGCTGA CTCTGTGCCA GCCAACACAG AAAACGAAGT TGAGCCTGTT GATGCCCGCC CTGCTGCCGA CCGAGGACTG ACCACTCGAC CAGGTTCTGG GTTGACAAAT ATCAAGACGG AGGAGATCTC TGAAGTGAAT CTGGATGCAG AATTCCGACA TGACTCAGGA TATGAAGTTC ATCATCAAAA ATTGGTGTTC TTTGCAGAAG ATGTGGGTTC AAACAAAGGT GCAATCATTG GACTCATGGT GGGCGGTGTT GTCATAGCGA CAGTGATCGT CATCACCTTG GTGATGCTGA AGAAGAAACA GTACACATCC ATTCATCATG GTGTGGTGGA GGTTGACGCC GCTGTCACCC CAGAGGAGCG CCACCTGTCC AAGATGCAGC AGAACGGCTA CGAAAATCCA ACCTACAAGT TCTTTGAGCA GATGCAGAAC TAGGATAACC GTATTACCGC CATGCATTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTAGAA TTCCTCGACG GATCCACCGG TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCGCCAC CGCGGTGGAG CTCGAATTAA TTCATCGATG ATGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCCTCTAGA TCAGATCCGA AGATACGGCC ACGGGTGCTC TTGATCCTGT GGCTGATTTT GGACTGTGCT GCTCGCAGCT GCTGATGAAT CACATACTTC CTCCATTTTC TTCCACTGAT TGACTGTTAT AATTTCCCTA ATTTCCAGGT CAAGGTGCTG TGCATTGTGG TAATAGATGT GACATGACGT CACTTCCAAA GGACCAATGA ACATGTCTGA CCAATTTCAT ATAATGTGAA AACGATTTTC ATAGGCAGAA TAAATAACAT TTAAATTAAA CTGGGCATCA GCGCAATTCA ATTGGTTTGG TAATAGCAAG GGAAAATAGA ATGAAGTGAT CTCCAAAAAA TAAGTACTTT TTGACTGTAA ATAAAATTGT AAGGAGTAAA AAGTACTTTT TTTTCTAAAA AAATGTAATT AAGTAAAAGT AAAAGTATTG ATTTTTAATT GTACTCAAGT AAAGTAAAAA TCCCCAAAAA TAATACTTAA GTACAGTAAT CAAGTAAAAT TACTCAAGTA CTTTACACCT CTGGTTCTTG ACCCCCTACC TTCAGCAAGC CCAGCAGATC CACTAGTTCT AGAGCGGCCG CCACCGCGGT GGAGCTCCAG CTTTTGTTCC CTTTAGTGAG GGTTAATTGC GCGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAG
