pADV-CMV-MCS-ZsGreen1
$70.00
In stock
SKU
PL51218
100μg liquid plasmid(Instantly):$91
| name | pADV-CMV-MCS-ZsGreen1 | another name | apADV-CMV-MCS-ZsGreen1 |
| Insert Size (bp) | Plasmid host | Mammalian cell,Adenovirus | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCCATTCGC CATTCAGGAT CGAATTAATT CTTAATTAAC ATCATCAATA ATATACCTTA TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ACTGTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGAGATCTGG TACCGTCGAC GCGGCCGCTC GAGCCTAAGC TTATGGCCCA GTCCAAGCAC GGCCTGACCA AGGAGATGAC CATGAAGTAC CGCATGGAGG GCTGCGTGGA CGGCCACAAG TTCGTGATCA CCGGCGAGGG CATCGGCTAC CCCTTCAAGG GCAAGCAGGC CATCAACCTG TGCGTGGTGG AGGGCGGCCC CTTGCCCTTC GCCGAGGACA TCTTGTCCGC CGCCTTCATG TACGGCAACC GCGTGTTCAC CGAGTACCCC CAGGACATCG TCGACTACTT CAAGAACTCC TGCCCCGCCG GCTACACCTG GGACCGCTCC TTCCTGTTCG AGGACGGCGC CGTGTGCATC TGCAACGCCG ACATCACCGT GAGCGTGGAG GAGAACTGCA TGTACCACGA GTCCAAGTTC TACGGCGTGA ACTTCCCCGC CGACGGCCCC GTGATGAAGA AGATGACCGA CAACTGGGAG CCCTCCTGCG AGAAGATCAT CCCCGTGCCC AAGCAGGGCA TCTTGAAGGG CGACGTGAGC ATGTACCTGC TGCTGAAGGA CGGTGGCCGC TTGCGCTGCC AGTTCGACAC CGTGTACAAG GCCAAGTCCG TGCCCCGCAA GATGCCCGAC TGGCACTTCA TCCAGCACAA GCTGACCCGC GAGGACCGCA GCGACGCCAA GAACCAGAAG TGGCACCTGA CCGAGCACGC CATCGCCTCC GGCTCCGCCT TGCCCTGAAT CCGATCCACC GGATCTAGAT AACTGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TAACGCGGAT CTGGGCGTGG TTAAGGGTGG GAAAGAATAT ATAAGGTGGG GGTCTTATGT AGTTTTGTAT CTGTTTTGCA GCAGCCGCCG CCGCCATGAG CACCAACTCG TTTGATGGAA GCATTGTGAG CTCATATTTG ACAACGCGCA TGCCCCCATG GGCCGGGGTG CGTCAGAATG TGATGGGCTC CAGCATTGAT GGTCGCCCCG TCCTGCCCGC AAACTCTACT ACCTTGACCT ACGAGACCGT GTCTGGAACG CCGTTGGAGA CTGCAGCCTC CGCCGCCGCT TCAGCCGCTG CAGCCACCGC CCGCGGGATT GTGACTGACT TTGCTTTCCT GAGCCCGCTT GCAAGCAGTG CAGCTTCCCG TTCATCCGCC CGCGATGACA AGTTGACGGC TCTTTTGGCA CAATTGGATT CTTTGACCCG GGAACTTAAT GTCGTTTCTC AGCAGCTGTT GGATCTGCGC CAGCAGGTTT CTGCCCTGAA GGCTTCCTCC CCTCCCAATG CGGTTTAAAA CATAAATAAA AAACCAGACT CTGTTTGGAT TTGGATCAAG CAAGTGTCTT GCTGTCTTTA TTTAGGGGTT TTGCGCGCGC GGTAGGCCCG GGACCAGCGG TCTCGGTCGT TGAGGGTCCT GTGTATTTTT TCCAGGACGT GGTAAAGGTG ACTCTGGATG TTCAGATACA TGGGCATAAG CCCGTCTCTG GGGTGGAGGT AGCACCACTG CAGAGCTTCA TGCTGCGGGG TGGTGTTGTA GATGATCCAG TCGTAGCAGG AGCGCTGGGC GTGGTGCCTA AAAATGTCTT TCAGTAGCAA GCTGATTGCC AGGGGCAGGC CCTTGGTGTA AGTGTTTACA AAGCGGTTAA GCTGGGATGG GTGCATACGT GGGGATATGA GATGCATCTT GGACTGTATT TTTAGGTTGG CTATGTTCCC AGCCATATCC CTCCGGGGAT TCATGTTGTG CAGAACCACC AGCACAGTGT ATCCGGTGCA CTTGGGAAAT TTGTCATGTA GCTTAGAAGG AAATGCGTGG AAGAACTTGG AGACGCCCTT GTGACCTCCA AGATTTTCCA TGCATTCGTC CATAATGATG GCAATGGGCC CACGGGCGGC GGCCTGGGCG AAGATATTTC TGGGATCACT AACGTCATAG TTGTGTTCCA GGATGAGATC GTCATAGGCC ATTTTTACAA AGCGCGGGCG GAGGGTGCCA GACTGCGGTA TAATGGTTCC ATCCGGCCCA GGGGCGTAGT TACCCTCACA GATTTGCATT TCCCACGCTT TGAGTTCAGA TGGGGGGATC ATGTCTACCT GCGGGGCGAT GAAGAAAACG GTTTCCGGGG TAGGGGAGAT CAGCTGGGAA GAAAGCAGGT TCCTGAGCAG CTGCGACTTA CCGCAGCCGG TGGGCCCGTA AATCACACCT ATTACCGGCT GCAACTGGTA GTTAAGAGAG CTGCAGCTGC CGTCATCCCT GAGCAGGGGG GCCACTTCGT TAAGCATGTC CCTGACTCGC ATGTTTTCCC TGACCAAATC CGCCAGAAGG CGCTCGCCGC CCAGCGATAG CAGTTCTTGC AAGGAAGCAA AGTTTTTCAA CGGTTTGAGA CCGTCCGCCG TAGGCATGCT TTTGAGCGTT TGACCAAGCA GTTCCAGGCG GTCCCACAGC TCGGTCACCT GCTCTACGGC ATCTCGATCC AGCATATCTC CTCGTTTCGC GGGTTGGGGC GGCTTTCGCT GTACGGCAGT AGTCGGTGCT CGTCCAGACG GGCCAGGGTC ATGTCTTTCC ACGGGCGCAG GGTCCTCGTC AGCGTAGTCT GGGTCACGGT GAAGGGGTGC GCTCCGGGCT GCGCGCTGGC CAGGGTGCGC TTGAGGCTGG TCCTGCTGGT GCTGAAGCGC TGCCGGTCTT CGCCCTGCGC GTCGGCCAGG TAGCATTTGA CCATGGTGTC ATAGTCCAGC CCCTCCGCGG CGTGGCCCTT GGCGCGCAGC TTGCCCTTGG AGGAGGCGCC GCACGAGGGG CAGTGCAGAC TTTTGAGGGC GTAGAGCTTG GGCGCGAGAA ATACCGATTC CGGGGAGTAG GCATCCGCGC CGCAGGCCCC GCAGACGGTC TCGCATTCCA CGAGCCAGGT GAGCTCTGGC CGTTCGGGGT CAAAAACCAG GTTTCCCCCA TGCTTTTTGA TGCGTTTCTT ACCTCTGGTT TCCATGAGCC GGTGTCCACG CTCGGTGACG AAAAGGCTGT CCGTGTCCCC GTATACAGAC TTGAGAGGGA GTTTAAACGA ATTCAATAGC TTGTTGCATG GGCGGCGATA TAAAATGCAA GGTGCTGCTC AAAAAATCAG GCAAAGCCTC GCGCAAAAAA GAAAGCACAT CGTAGTCATG CTCATGCAGA TAAAGGCAGG TAAGCTCCGG AACCACCACA GAAAAAGACA CCATTTTTCT CTCAAACATG TCTGCGGGTT TCTGCATAAA CACAAAATAA AATAACAAAA AAACATTTAA ACATTAGAAG CCTGTCTTAC AACAGGAAAA ACAACCCTTA TAAGCATAAG ACGGACTACG GCCATGCCGG CGTGACCGTA AAAAAACTGG TCACCGTGAT TAAAAAGCAC CACCGACAGC TCCTCGGTCA TGTCCGGAGT CATAATGTAA GACTCGGTAA ACACATCAGG TTGATTCACA TCGGTCAGTG CTAAAAAGCG ACCGAAATAG CCCGGGGGAA TACATACCCG CAGGCGTAGA GACAACATTA CAGCCCCCAT AGGAGGTATA ACAAAATTAA TAGGAGAGAA AAACACATAA ACACCTGAAA AACCCTCCTG CCTAGGCAAA ATAGCACCCT CCCGCTCCAG AACAACATAC AGCGCTTCCA CAGCGGCAGC CATAACAGTC AGCCTTACCA GTAAAAAAGA AAACCTATTA AAAAAACACC ACTCGACACG GCACCAGCTC AATCAGTCAC AGTGTAAAAA AGGGCCAAGT GCAGAGCGAG TATATATAGG ACTAAAAAAT GACGTAACGG TTAAAGTCCA CAAAAAACAC CCAGAAAACC GCACGCGAAC CTACGCCCAG AAACGAAAGC CAAAAAACCC ACAACTTCCT CAAATCGTCA CTTCCGTTTT CCCACGTTAC GTCACTTCCC ATTTTAAGAA AACTACAATT CCCAACACAT ACAAGTTACT CCGCCCTAAA ACCTACGTCA CCCGCCCCGT TCCCACGCCC CGCGCCACGT CACAAACTCC ACCCCCTCAT TATCATATTG GCTTCAATCC AAAATAAGGT ATATTATTGA TGATGTTAAT TAACATGCAT GGATCCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTG CAGCCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTCACGTAG AAAGCCAGTC CGCAGAAACG GTGCTGACCC CGGATGAATG TCAGCTACTG GGCTATCTGG ACAAGGGAAA ACGCAAGCGC AAAGAGAAAG CAGGTAGCTT GCAGTGGGCT TACATGGCGA TAGCTAGACT GGGCGGTTTT ATGGACAGCA AGCGAACCGG AATTGCCAGC TGGGGCGCCC TCTGGTAAGG TTGGGAAGCC CTGCAAAGTA AACTGGATGG CTTTCTTGCC GCCAAGGATC TGATGGCGCA GGGGATCAAG CTCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAA TTTTGTTAAA ATTTTTGTTA AATCA
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