pBAD-Gam-Beta-Exo-Spe
$113.00
In stock
SKU
PL51206
pKD46-Spe
100μg liquid plasmid(Instantly):$134
| name | pBAD-Gam-Beta-Exo-Spe | another name | apBAD-Gam-Beta-Exo-Spe |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | Red |
| Fragment species | protease | Prokaryotic resistance | Spe |
| Selective marker | Fluorescent labeling | ||
| Promoter | BAD | Replicon | pSC101 |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGCACGATC AACGGCACTG TTGCAAATAG TCGGTGGTGA TAAACTTATC ATCCCCTTTT GCTTATGGAG CTGCACATGA ACTCACATTT CCCCGAAAAG TGCCACCTGC ATCGATTTAT TATGACAACT TGACGGCTAC ATCATTCACT TTTTCTTCAC AACCGGCACG GAACTCGCTC GGGCTGGCCC CGGTGCATTT TTTAAATACC CGCGAGAAAT AGAGTTGATC GTCAAAACCA ACATTGCGAC CGACGGTGGC GATAGGCATC CGGGTGGTGC TCAAAAGCAG CTTCGCCTGG CTGATACGTT GGTCCTCGCG CCAGCTTAAG ACGCTAATCC CTAACTGCTG GCGGAAAAGA TGTGACAGAC GCGACGGCGA CAAGCAAACA TGCTGTGCGA CGCTGGCGAT ATCAAAATTG CTGTCTGCCA GGTGATCGCT GATGTACTGA CAAGCCTCGC GTACCCGATT ATCCATCGGT GGATGGAGCG ACTCGTTAAT CGCTTCCATG CGCCGCAGTA ACAATTGCTC AAGCAGATTT ATCGCCAGCA GCTCCGAATA GCGCCCTTCC CCTTGCCCGG CGTTAATGAT TTGCCCAAAC AGGTCGCTGA AATGCGGCTG GTGCGCTTCA TCCGGGCGAA AGAACCCCGT ATTGGCAAAT ATTGACGGCC AGTTAAGCCA TTCATGCCAG TAGGCGCGCG GACGAAAGTA AACCCACTGG TGATACCATT CGCGAGCCTC CGGATGACGA CCGTAGTGAT GAATCTCTCC TGGCGGGAAC AGCAAAATAT CACCCGGTCG GCAAACAAAT TCTCGTCCCT GATTTTTCAC CACCCCCTGA CCGCGAATGG TGAGATTGAG AATATAACCT TTCATTCCCA GCGGTCGGTC GATAAAAAAA TCGAGATAAC CGTTGGCCTC AATCGGCGTT AAACCCGCCA CCAGATGGGC ATTAAACGAG TATCCCGGCA GCAGGGGATC ATTTTGCGCT TCAGCCATAC TTTTCATACT CCCGCCATTC AGAGAAGAAA CCAATTGTCC ATATTGCATC AGACATTGCC GTCACTGCGT CTTTTACTGG CTCTTCTCGC TAACCAAACC GGTAACCCCG CTTATTAAAA GCATTCTGTA ACAAAGCGGG ACCAAAGCCA TGACAAAAAC GCGTAACAAA AGTGTCTATA ATCACGGCAG AAAAGTCCAC ATTGATTATT TGCACGGCGT CACACTTTGC TATGCCATAG CATTTTTATC CATAAGATTA GCGGATCCTA CCTGACGCTT TTTATCGCAA CTCTCTACTG TTTCTCCATA CCCGTTTTTT TGGGAATTCG AGCTCTAAGG AGGTTATAAA AAATGGATAT TAATACTGAA ACTGAGATCA AGCAAAAGCA TTCACTAACC CCCTTTCCTG TTTTCCTAAT CAGCCCGGCA TTTCGCGGGC GATATTTTCA CAGCTATTTC AGGAGTTCAG CCATGAACGC TTATTACATT CAGGATCGTC TTGAGGCTCA GAGCTGGGCG CGTCACTACC AGCAGCTCGC CCGTGAAGAG AAAGAGGCAG AACTGGCAGA CGACATGGAA AAAGGCCTGC CCCAGCACCT GTTTGAATCG CTATGCATCG ATCATTTGCA ACGCCACGGG GCCAGCAAAA AATCCATTAC CCGTGCGTTT GATGACGATG TTGAGTTTCA GGAGCGCATG GCAGAACACA TCCGGTACAT GGTTGAAACC ATTGCTCACC ACCAGGTTGA TATTGATTCA GAGGTATAAA ACGAATGAGT ACTGCACTCG CAACGCTGGC TGGGAAGCTG GCTGAACGTG TCGGCATGGA TTCTGTCGAC CCACAGGAAC TGATCACCAC TCTTCGCCAG ACGGCATTTA AAGGTGATGC CAGCGATGCG CAGTTCATCG CATTACTGAT CGTTGCCAAC CAGTACGGCC TTAATCCGTG GACGAAAGAA ATTTACGCCT TTCCTGATAA GCAGAATGGC ATCGTTCCGG TGGTGGGCGT TGATGGCTGG TCCCGCATCA TCAATGAAAA CCAGCAGTTT GATGGCATGG ACTTTGAGCA GGACAATGAA TCCTGTACAT GCCGGATTTA CCGCAAGGAC CGTAATCATC CGATCTGCGT TACCGAATGG ATGGATGAAT GCCGCCGCGA ACCATTCAAA ACTCGCGAAG GCAGAGAAAT CACGGGGCCG TGGCAGTCGC ATCCCAAACG GATGTTACGT CATAAAGCCA TGATTCAGTG TGCCCGTCTG GCCTTCGGAT TTGCTGGTAT CTATGACAAG GATGAAGCCG AGCGCATTGT CGAAAATACT GCATACACTG CAGAACGTCA GCCGGAACGC GACATCACTC CGGTTAACGA TGAAACCATG CAGGAGATTA ACACTCTGCT GATCGCCCTG GATAAAACAT GGGATGACGA CTTATTGCCG CTCTGTTCCC AGATATTTCG CCGCGACATT CGTGCATCGT CAGAACTGAC ACAGGCCGAA GCAGTAAAAG CTCTTGGATT CCTGAAACAG AAAGCCGCAG AGCAGAAGGT GGCAGCATGA CACCGGACAT TATCCTGCAG CGTACCGGGA TCGATGTGAG AGCTGTCGAA CAGGGGGATG ATGCGTGGCA CAAATTACGG CTCGGCGTCA TCACCGCTTC AGAAGTTCAC AACGTGATAG CAAAACCCCG CTCCGGAAAG AAGTGGCCTG ACATGAAAAT GTCCTACTTC CACACCCTGC TTGCTGAGGT TTGCACCGGT GTGGCTCCGG AAGTTAACGC TAAAGCACTG GCCTGGGGAA AACAGTACGA GAACGACGCC AGAACCCTGT TTGAATTCAC TTCCGGCGTG AATGTTACTG AATCCCCGAT CATCTATCGC GACGAAAGTA TGCGTACCGC CTGCTCTCCC GATGGTTTAT GCAGTGACGG CAACGGCCTT GAACTGAAAT GCCCGTTTAC CTCCCGGGAT TTCATGAAGT TCCGGCTCGG TGGTTTCGAG GCCATAAAGT CAGCTTACAT GGCCCAGGTG CAGTACAGCA TGTGGGTGAC GCGAAAAAAT GCCTGGTACT TTGCCAACTA TGACCCGCGT ATGAAGCGTG AAGGCCTGCA TTATGTCGTG ATTGAGCGGG ATGAAAAGTA CATGGCGAGT TTTGACGAGA TCGTGCCGGA GTTCATCGAA AAAATGGACG AGGCACTGGC TGAAATTGGT TTTGTATTTG GGGAGCAATG GCGATGACGC ATCCTCACGA TAATATCCGG GTAGGCGCAA TCACTTTCGT CTACTCCGTT ACAAAGCGAG GCTGGGTATT TCCCGGCCTT TCTGTTATCC GAAATCCACT GAAAGCACAG CGGCTGGCTG AGGAGATAAA TAATAAACGA GGGGCTGTAT GCACAAAGCA TCTTCTGTTG AGTTAAGAAC GAGTATCGAG ATGGCACATA GCCTTGCTCA AATTGGAATC AGGTTTGTGC CAATACCAGT AGAAACAGAC GAAGAATCCA TGGGTATGGA CAGTTTTCCC TTTGATATGT AACGGTGAAC AGTTGTTCTA CTTTTGTTTG TTAGTCTTGA TGCTTCACTG ATAGATACAA GAGCCATAAG AACCTCAGAT CCTTCCGTAT TTAGCCAGTA TGTTCTCTAG TGTGGTTCGT TGTTTTTGCG TGAGCCATGA GAACGAACCA TTGAGATCAT ACTTACTTTG CATGTCACTC AAAAATTTTG CCTCAAAACT GGTGAGCTGA ATTTTTGCAG TTAAAGCATC GTGTAGTGTT TTTCTTAGTC CGTTACGTAG GTAGGAATCT GATGTAATGG TTGTTGGTAT TTTGTCACCA TTCATTTTTA TCTGGTTGTT CTCAAGTTCG GTTACGAGAT CCATTTGTCT ATCTAGTTCA ACTTGGAAAA TCAACGTATC AGTCGGGCGG CCTCGCTTAT CAACCACCAA TTTCATATTG CTGTAAGTGT TTAAATCTTT ACTTATTGGT TTCAAAACCC ATTGGTTAAG CCTTTTAAAC TCATGGTAGT TATTTTCAAG CATTAACATG AACTTAAATT CATCAAGGCT AATCTCTATA TTTGCCTTGT GAGTTTTCTT TTGTGTTAGT TCTTTTAATA ACCACTCATA AATCCTCATA GAGTATTTGT TTTCAAAAGA CTTAACATGT TCCAGATTAT ATTTTATGAA TTTTTTTAAC TGGAAAAGAT AAGGCAATAT CTCTTCACTA AAAACTAATT CTAATTTTTC GCTTGAGAAC TTGGCATAGT TTGTCCACTG GAAAATCTCA AAGCCTTTAA CCAAAGGATT CCTGATTTCC ACAGTTCTCG TCATCAGCTC TCTGGTTGCT TTAGCTAATA CACCATAAGC ATTTTCCCTA CTGATGTTCA TCATCTGAGC GTATTGGTTA TAAGTGAACG ATACCGTCCG TTCTTTCCTT GTAGGGTTTT CAATCGTGGG GTTGAGTAGT GCCACACAGC ATAAAATTAG CTTGGTTTCA TGCTCCGTTA AGTCATAGCG ACTAATCGCT AGTTCATTTG CTTTGAAAAC AACTAATTCA GACATACATC TCAATTGGTC TAGGTGATTT TAATCACTAT ACCAATTGAG ATGGGCTAGT CAATGATAAT TACTAGTCCT TTTCCTTTGA GTTGTGGGTA TCTGTAAATT CTGCTAGACC TTTGCTGGAA AACTTGTAAA TTCTGCTAGA CCCTCTGTAA ATTCCGCTAG ACCTTTGTGT GTTTTTTTTG TTTATATTCA AGTGGTTATA ATTTATAGAA TAAAGAAAGA ATAAAAAAAG ATAAAAAGAA TAGATCCCAG CCCTGTGTAT AACTCACTAC TTTAGTCAGT TCCGCAGTAT TACAAAAGGA TGTCGCAAAC GCTGTTTGCT CCTCTACAAA ACAGACCTTA AAACCCTAAA GGCTTAAGTA GCACCCTCGC AAGCTCGGTT GCGGCCGCAA TCGGGCAAAT CGCTGAATAT TCCTTTTGTC TCCGACCATC AGGCACCTGA GTCGCTGTCT TTTTCGTGAC ATTCAGTTCG CTGCGCTCAC GGCTCTGGCA GTGAATGGGG GTAAATGGCA CTACAGGCGC CTTTTATGGA TTCATGCAAG GAAACTACCC ATAATACAAG AAAAGCCCGT CACGGGCTTC TCAGGGCGTT TTATGGCGGG TCTGCTATGT GGTGCTATCT GACTTTTTGC TGTTCAGCAG TTCCTGCCCT CTGATTTTCC AGTCTGACCA CTTCGGATTA TCCCGTGACA GGTCATTCAG ACTGGCTAAT GCACCCAGTA AGGCAGCGGT ATCATCAACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGCTTC ACCTAGATCC TTTACGCGTT CGCGGAACTT CGGAATAGGA ACTTCATGAG CTCAGCCAAT CGACTGGCGA GCGGCATCTT ATTTGCCGAC TACCTTGGTG ATCTCGCCTT TCACGTAGTG GACAAATTCT TCCAACTGAT CTGCGCGCGA GGCCAAGCGA TCTTCTTCTT GTCCAAGATA AGCCTGTCTA GCTTCAAGTA TGACGGGCTG ATACTGGGCC GGCAGGCGCT CCATTGCCCA GTCGGCAGCG ACATCCTTCG GCGCGATTTT GCCGGTTACT GCGCTGTACC AAATGCGGGA CAACGTAAGC ACTACATTTC GCTCATCGCC AGCCCAGTCG GGCGGCGAGT TCCATAGCGT TAAGGTTTCA TTTAGCGCCT CAAATAGATC CTGTTCAGGA ACCGGATCAA AGAGTTCCTC CGCCGCTGGA CCTACCAAGG CAACGCTATG TTCTCTTGCT TTTGTCAGCA AGATAGCCAG ATCAATGTCG ATCGTGGCTG GCTCGAAGAT ACCTGCAAGA ATGTCATTGC GCTGCCATTC TCCAAATTGC AGTTCGCGCT TAGCTGGATA ACGCCACGGA ATGATGTCGT CGTGCACAAC AATGGTGACT TCTACAGCGC GGAGAATCTC GCTCTCTCCA GGGGAAGCCG AAGTTTCCAA AAGGTCGTTG ATCAAAGCTC GCCGCGTTGT TTCATCAAGC CTTACGGTCA CCGTAACCAG CAAATCAATA TCACTGTGTG GCTTCAGGCC GCCATCCACT GCGGAGCCGT ACAAATGTAC GGCCAGCAAC GTCGGTTCGA GATGGCGCTC GATGACGCCA ACTACCTCTG ATAGTTGAGT CGATACTTCG GCGATCACCG CTTCCCTCAT GACATTGCAC TCCACCGCTG ATGACATCAG TCGATCA
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