pCMV-HA-HK2(human)-K49R-SV40-Neo
$84.00
In stock
SKU
PL51193
NM_000189.5;HKII; HXK2
100μg liquid plasmid(Instantly):$105
| name | pCMV-HA-HK2(human)-K49R-SV40-Neo | another name | apCMV-HA-HK2(human)-K49R-SV40-Neo |
| Insert Size (bp) | 2754bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCGC CACCATGTAC CCATACGACG TCCCAGACTA CGCTGGAAGC TTGGTACCGA TGATTGCCTC GCATCTGCTT GCCTACTTCT TCACGGAGCT CAACCATGAC CAAGTGCAGA AGGTTGACCA GTATCTCTAC CACATGCGCC TCTCTGATGA GACCCTCTTG GAGATCTCTA AGCGGTTCCG CAAGGAGATG GAGCGGGGGC TTGGAGCCAC CACTCACCCT ACTGCAGCAG TGAAGATGCT GCCCACCTTT GTGAGGTCCA CTCCAGATGG GACAGAACAC GGAGAGTTCC TGGCTCTGGA TCTTGGAGGG ACCAACTTCC GTGTGCTTTG GGTGAAAGTA ACGGACAATG GGCTCCAGAA GGTGGAGATG GAGAATCAGA TCTATGCCAT CCCTGAGGAC ATCATGCGAG GCAGTGGCAC CCAGCTGTTT GACCACATTG CCGAATGCCT GGCTAACTTC ATGGATAAGC TACAAATCAA AGACAAGAAG CTCCCACTGG GTTTTACCTT CTCGTTCCCC TGCCACCAGA CTAAACTAGA CGAGAGTTTC CTGGTCTCAT GGACCAAGGG ATTCAAGTCC AGTGGAGTGG AAGGCAGAGA CGTTGTGGCT CTGATCCGGA AGGCCATCCA GAGGAGAGGG GACTTTGATA TCGACATTGT GGCTGTGGTG AATGACACAG TTGGGACCAT GATGACCTGT GGTTATGATG ACCACAACTG TGAGATTGGT CTCATTGTGG GCACGGGCAG CAACGCCTGC TACATGGAAG AGATGCGCCA CATCGACATG GTGGAAGGCG ATGAGGGGCG GATGTGTATC AATATGGAGT GGGGGGCCTT CGGGGACGAT GGCTCGCTCA ACGACATTCG CACTGAGTTT GACCAGGAGA TTGACATGGG CTCACTGAAC CCGGGAAAGC AACTGTTTGA GAAGATGATC AGTGGGATGT ACATGGGGGA GCTGGTGAGG CTTATCCTGG TGAAGATGGC CAAGGAGGAG CTGCTCTTTG GGGGGAAGCT CAGCCCAGAG CTTCTCAACA CCGGTCGCTT TGAGACCAAA GACATCTCAG ACATTGAAGG GGAGAAGGAT GGCATCCGGA AGGCCCGTGA GGTCCTGATG CGGTTGGGCC TGGACCCGAC TCAGGAGGAC TGCGTGGCCA CTCACCGGAT CTGCCAGATC GTGTCCACAC GCTCCGCCAG CCTGTGCGCA GCCACCCTGG CCGCCGTGCT GCAGCGCATC AAGGAGAACA AAGGCGAGGA GCGGCTGCGC TCTACTATTG GGGTCGACGG TTCCGTCTAC AAGAAACACC CCCATTTTGC CAAGCGTCTA CATAAGACCG TGCGGCGGCT GGTGCCCGGC TGCGATGTCC GCTTCCTCCG CTCCGAGGAT GGCAGTGGCA AAGGTGCAGC CATGGTGACA GCAGTGGCTT ACCGGCTGGC CGATCAACAC CGTGCCCGCC AGAAGACATT AGAGCATCTG CAGCTGAGCC ATGACCAGCT GCTGGAGGTC AAGAGGAGGA TGAAGGTAGA AATGGAGCGA GGTCTGAGCA AGGAGACTCA TGCCAGTGCC CCCGTCAAGA TGCTGCCCAC CTACGTGTGT GCTACCCCGG ACGGCACAGA GAAAGGGGAC TTCTTGGCCT TGGACCTTGG AGGAACAAAT TTCCGGGTCC TGCTGGTCCG TGTTCGGAAT GGGAAGTGGG GTGGAGTGGA GATGCACAAC AAGATCTACG CCATCCCGCA GGAGGTCATG CACGGCACCG GGGACGAGCT CTTTGACCAC ATTGTCCAGT GCATCGCGGA CTTCCTCGAG TACATGGGCA TGAAGGGCGT GTCCCTGCCT CTGGGTTTTA CCTTCTCCTT CCCCTGCCAG CAGAACAGCC TGGACGAGAG CATCCTCCTC AAGTGGACAA AAGGCTTCAA GGCATCTGGC TGCGAGGGCG AGGACGTGGT GACCCTGCTG AAGGAAGCGA TCCACCGGCG AGAGGAGTTT GACCTGGATG TGGTTGCTGT GGTGAACGAC ACAGTCGGAA CTATGATGAC CTGTGGCTTT GAAGACCCTC ACTGTGAAGT TGGCCTCATT GTTGGCACGG GCAGCAATGC CTGCTACATG GAGGAGATGC GCAACGTGGA ACTGGTGGAA GGAGAAGAGG GGCGGATGTG TGTGAACATG GAATGGGGGG CCTTCGGGGA CAATGGATGC CTAGATGACT TCCGCACAGA ATTTGATGTG GCTGTGGATG AGCTTTCACT CAACCCCGGC AAGCAGAGGT TCGAGAAAAT GATCAGTGGA ATGTACCTGG GTGAGATTGT CCGTAACATT CTCATCGATT TCACCAAGCG TGGACTACTC TTCCGAGGCC GCATCTCAGA GCGGCTCAAG ACAAGGGGCA TCTTTGAAAC CAAGTTCTTG TCTCAGATTG AGAGTGACTG CCTGGCCCTG CTGCAAGTCC GAGCCATCCT GCAACACTTA GGGCTTGAGA GCACCTGTGA CGACAGCATC ATTGTTAAGG AGGTGTGCAC TGTGGTGGCC CGGCGGGCAG CCCAGCTCTG TGGCGCAGGC ATGGCCGCTG TGGTGGACAG GATACGAGAA AACCGTGGGC TGGACGCTCT CAAAGTGACA GTGGGTGTGG ATGGGACCCT CTACAAGCTA CATCCTCACT TTGCCAAAGT CATGCATGAG ACAGTGAAGG ACCTGGCTCC GAAATGTGAT GTGTCTTTCC TGCAGTCAGA GGATGGCAGC GGGAAGGGGG CGGCGCTCAT CACTGCTGTG GCCTGCCGCA TCCGTGAGGC TGGACAGCGA TAGGAATTCT GCAGATATCC ATCACACTGG CGGCCGCTCG AGCATGCATC TAGAGGGCCC TATTCTATAG TGTCACCTAA ATGCTAGAGC TCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGCATCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGGG GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TC
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