pU6-NR4A2(mouse)-sgRNA1-Cas9-mCherry-Puro
100μg liquid plasmid(Instantly):$91
100μg liquid plasmid(Instantly):$91
| name | pU6-NR4A2(mouse)-sgRNA1-Cas9-mCherry-Puro | another name | apU6-NR4A2(mouse)-sgRNA1-Cas9-mCherry-Puro |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | U6 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGACACAATT GCATGAAGAA TCTGCTTAGG GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTTG CCACCATGGA CAAGAAGTAC AGCATCGGCC TGGACATCGG TACCAACAGC GTGGGCTGGG CCGTGATCAC CGACGAGTAC AAGGTGCCCA GCAAGAAGTT CAAGGTGCTG GGCAACACCG ACCGCCACAG CATCAAGAAG AACCTGATCG GCGCCCTGCT GTTCGACAGC GGCGAGACCG CCGAGGCCAC CCGCCTGAAG CGCACCGCCC GCCGCCGCTA CACCCGCCGC AAGAACCGCA TCTGCTACCT GCAGGAGATC TTCAGCAACG AGATGGCCAA GGTGGACGAC AGCTTCTTCC ACCGCCTGGA GGAGAGCTTC CTGGTGGAGG AGGACAAGAA GCACGAGCGC CACCCCATCT TCGGCAACAT CGTGGACGAG GTGGCCTACC ACGAGAAGTA CCCCACCATC TACCACCTGC GCAAGAAGCT GGTGGACAGC ACCGACAAGG CCGACCTGCG CCTGATCTAC CTGGCCCTGG CCCACATGAT CAAGTTCCGC GGCCACTTCC TGATCGAGGG CGACCTGAAC CCCGACAACA GCGACGTGGA CAAGCTGTTC ATCCAGCTGG TGCAGACCTA CAACCAGCTG TTCGAGGAGA ACCCCATCAA CGCCAGCGGC GTGGACGCCA AGGCCATCCT GAGCGCCCGC CTGAGCAAGA GCCGCCGCCT GGAGAACCTG ATCGCCCAGC TGCCCGGCGA GAAGAAGAAC GGCCTGTTCG GCAACCTGAT CGCCCTGAGC CTGGGCCTGA CCCCCAACTT CAAGAGCAAC TTCGACCTGG CCGAGGACGC CAAGCTGCAG CTGAGCAAGG ACACCTACGA CGACGACCTG GACAACCTGC TGGCCCAGAT CGGCGACCAG TACGCCGACC TGTTCCTGGC CGCCAAGAAC CTGAGCGACG CCATCCTGCT GAGCGACATC CTGCGCGTGA ACACCGAGAT CACCAAGGCC CCCCTGAGCG CCAGCATGAT CAAGCGCTAC GACGAGCACC ACCAGGACCT GACCCTGCTG AAGGCCCTGG TGCGCCAGCA GCTGCCCGAG AAGTACAAGG AGATCTTCTT CGACCAGAGC AAGAACGGCT ACGCCGGCTA CATCGACGGC GGCGCCAGCC AGGAGGAGTT CTACAAGTTC ATCAAGCCCA TCCTGGAGAA GATGGACGGC ACCGAGGAGC TGCTGGTGAA GCTGAACCGC GAGGACCTGC TGCGCAAGCA GCGCACCTTC GACAACGGCA GCATCCCCCA CCAGATCCAC CTGGGCGAGC TGCACGCCAT CCTGCGCCGC CAGGAGGACT TCTACCCCTT CCTGAAGGAC AACCGCGAGA AGATCGAGAA GATCCTGACC TTCCGCATCC CCTACTACGT GGGCCCCCTG GCCCGCGGCA ACAGCCGCTT CGCCTGGATG ACCCGCAAGA GCGAGGAGAC CATCACCCCC TGGAACTTCG AGGAGGTGGT GGACAAGGGC GCCAGCGCCC AGAGCTTCAT CGAGCGCATG ACCAACTTCG ACAAGAACCT GCCCAACGAG AAGGTGCTGC CCAAGCACAG CCTGCTGTAC GAGTACTTCA CCGTGTACAA CGAGCTGACC AAGGTGAAGT ACGTGACCGA GGGCATGCGC AAGCCCGCCT TCCTGAGCGG CGAGCAGAAG AAGGCCATCG TGGACCTGCT GTTCAAGACC AACCGCAAGG TGACCGTGAA GCAGCTGAAG GAGGACTACT TCAAGAAGAT CGAGTGCTTC GACAGCGTGG AGATCAGCGG CGTGGAGGAC CGCTTCAACG CCAGCCTGGG CACCTACCAC GACCTGCTGA AGATCATCAA GGACAAGGAC TTCCTGGACA ACGAGGAGAA CGAGGACATC CTGGAGGACA TCGTGCTGAC CCTGACCCTG TTCGAGGACC GCGAGATGAT CGAGGAGCGC CTGAAGACCT ACGCCCACCT GTTCGACGAC AAGGTGATGA AGCAGCTGAA GCGCCGCCGC TACACCGGCT GGGGCCGCCT GAGCCGCAAG CTTATCAACG GCATCCGCGA CAAGCAGAGC GGCAAGACCA TCCTGGACTT CCTGAAGAGC GACGGCTTCG CCAACCGCAA CTTCATGCAG CTGATCCACG ACGACAGCCT GACCTTCAAG GAGGACATCC AGAAGGCCCA GGTGAGCGGC CAGGGCGACA GCCTGCACGA GCACATCGCC AACCTGGCCG GCAGCCCCGC CATCAAGAAG GGCATCCTGC AGACCGTGAA GGTGGTGGAC GAGCTGGTGA AGGTGATGGG CCGCCACAAG CCCGAGAACA TCGTGATCGA GATGGCCCGC GAGAACCAGA CCACCCAGAA GGGCCAGAAG AACAGCCGCG AGCGCATGAA GCGCATCGAG GAGGGCATCA AGGAGCTGGG CAGCCAGATC CTGAAGGAGC ACCCCGTGGA GAACACCCAG CTGCAGAACG AGAAGCTGTA CCTGTACTAC CTGCAGAACG GCCGCGACAT GTACGTGGAC CAGGAGCTGG ACATCAACCG CCTGAGCGAC TACGACGTGG ACCACATCGT GCCCCAGAGC TTCCTGAAGG ACGACAGCAT CGACAACAAG GTGCTGACCC GCAGCGACAA GAACCGCGGC AAGAGCGACA ACGTGCCCAG CGAGGAGGTG GTGAAGAAGA TGAAGAACTA CTGGCGCCAG CTGCTGAACG CCAAGCTGAT CACCCAGCGC AAGTTCGACA ACCTGACCAA GGCCGAGCGC GGCGGCCTGA GCGAGCTGGA CAAGGCCGGC TTCATCAAGC GCCAGCTGGT GGAGACCCGC CAGATCACCA AGCACGTGGC CCAGATCCTG GACAGCCGCA TGAACACCAA GTACGACGAG AACGACAAGC TGATCCGCGA GGTGAAGGTG ATCACCCTGA AGAGCAAGCT GGTGAGCGAC TTCCGCAAGG ACTTCCAGTT CTACAAGGTG CGCGAGATCA ACAACTACCA CCACGCCCAC GACGCCTACC TGAACGCCGT GGTGGGCACC GCCCTGATCA AGAAGTACCC CAAGCTGGAG AGCGAGTTCG TGTACGGCGA CTACAAGGTG TACGACGTGC GCAAGATGAT CGCCAAGAGC GAGCAGGAGA TCGGCAAGGC CACCGCCAAG TACTTCTTCT ACAGCAACAT CATGAACTTC TTCAAGACCG AGATCACCCT GGCCAACGGC GAGATCCGCA AGCGCCCCCT GATCGAGACC AACGGCGAGA CCGGCGAGAT CGTGTGGGAC AAGGGCCGCG ACTTCGCCAC CGTGCGCAAG GTGCTGAGCA TGCCCCAGGT GAACATCGTG AAGAAGACCG AGGTGCAGAC CGGCGGCTTC AGCAAGGAGA GCATCCTGCC CAAGCGCAAC AGCGACAAGC TGATCGCCCG CAAGAAGGAC TGGGACCCCA AGAAGTACGG CGGCTTCGAC AGCCCCACCG TGGCCTACAG CGTGCTGGTG GTGGCCAAGG TGGAGAAGGG CAAGAGCAAG AAGCTGAAGA GCGTGAAGGA GCTGCTGGGC ATCACCATCA TGGAGCGCAG CAGCTTCGAG AAGAACCCCA TCGACTTCCT GGAGGCCAAG GGCTACAAGG AGGTGAAGAA GGACCTGATC ATCAAGCTGC CCAAGTACAG CCTGTTCGAG CTGGAGAACG GCCGCAAGCG CATGCTGGCC AGCGCCGGCG AGCTGCAGAA GGGCAACGAG CTGGCCCTGC CCAGCAAGTA CGTGAACTTC CTGTACCTGG CCAGCCACTA CGAGAAGCTG AAGGGCAGCC CCGAGGACAA CGAGCAGAAG CAGCTGTTCG TGGAGCAGCA CAAGCACTAC CTGGACGAGA TCATCGAGCA GATCAGCGAG TTCAGCAAGC GCGTGATCCT GGCCGACGCC AACCTGGACA AGGTGCTGAG CGCCTACAAC AAGCACCGCG ACAAGCCCAT CCGCGAGCAG GCCGAGAACA TCATCCACCT GTTCACCCTG ACCAACCTGG GCGCCCCCGC CGCCTTCAAG TACTTCGACA CCACCATCGA CCGCAAGCGC TACACCAGCA CCAAGGAGGT GCTGGACGCC ACCCTGATCC ACCAGAGCAT CACCGGTCTG TACGAGACCC GCATCGACCT GAGCCAGCTG GGCGGCGACG GCGGCTCCGG ACCTCCAAAG AAAAAGAGAA AAGTATACCC CTACGACGTG CCCGACTACG CCTAATAACT CGAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCGGCGCGC CATACCGGTC GCCACCATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCTGAGG TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG ACGAGCTGTA CAAGTAGGCG GCCGCATCTA GGGCGGCCAA TTCCGCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTGATTTTCC ACCATATTGC CGTCTTTTGG CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA AGCTTCTTGA AGACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGTATAA GATACACCTG CAAAGGCGGC ACAACCCCAG TGCCACGTTG TGGGTTGGAT AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC AAGCGTATTC AACAAGGGGC TGAAGGATGC CCAGAAGGTA CCCCATTGTA TGGGATCTGA TCTGGGGCCT CGGTGCACAT GCTTTACATG TGTTTAGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAG CTTGCCACAA CCCACAAGAC CTTCCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCCGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGACC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GTGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGACGCCC GCCCCACGAC CCGCAGCGCC CGACCGAAAG GAGCGCACGA CCCCATGGCT CCGACCGAAG CCACCCGGGG CGGCCCCGCC GACCCCGCAC CCGCCCCCGA GGCCCACCGA CTCTAGATAA CTGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA ACGCGTCGAG TGCATTCTAG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGGTAAG CCTGAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTTTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACATGA TTACGAATTG CAACGATTTA GGTGACACTA TAGAAGAGAA GGAATTAATA CGACTCACTA TAGGGAGAGA GAGAGAATTA CCCTCACTAA AGGGAGGAGA AGCATGAATT CCCCAGTGGA AAGACGCGCA GGCAAAACGC ACCACGTGAC GGAGCGTGAC CGCGCGCCGA GCGCGCGCCA AGGTCGGGCA GGAAGAGGGC CTATTTCCCA TGATTCCTTC ATATTTGCAT ATACGATACA AGGCTGTTAG AGAGATAATT AGAATTAATT TGACTGTAAA CACAAAGATA TTAGTACAAA ATACGTGACG TAGAAAGTAA TAATTTCTTG GGTAGTTTGC AGTTTTAAAA TTATGTTTTA AAATGGACTA TCATATGCTT ACCGTAACTT GAAAGTATTT CGATTTCTTG GGTTTATATA TCTTGTGGAA AGGACGAGGA TCCGCAACTT CCACCAGAAC TACGGTTTTA GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC GAGTCGGTGC TTTTTTCT