pCMV-SETDB2(rat)-IRES-EGFP-Puro
$99.00
In stock
SKU
PL50771
100μg liquid plasmid(Instantly):$120
| name | pCMV-SETDB2(rat)-IRES-EGFP-Puro | another name | apCMV-SETDB2(rat)-IRES-EGFP-Puro |
| Insert Size (bp) | 3027bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Rat | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCCACC ATGGAAGAAA AAATTGGTGA TGCAAAGACT TTCTGGATGG AGCTACAAGA TGATGGTAAA GTTGACTTTA TGTTTGAGAA AGCACAAGAT GTCCTACATT CACTGAAACA GAAGATAAAA GATGGGTCTG CCACAAATGG AGACTATGTC CAAGCAATGG TTCTGGTAAA CGAAGCAACT CTGAGTAACA GTCAGACACT GGAAAAAGAT CATACACCTG TGACTCAGAA TGAACAGAAA AACAAATCAA ATGTGTTTCC CTCTACATCA TGTGAAAACC CCTCCCCTGA GGACTGTATC ATTCTATCTA CAGGAAGAAA AGGATTCCTC CCTGTGAACA ATAAAACTGA AGGTTCGGGG GAGAAGGAGC CCCCTTTGCA TGCATCTTTC CATCACCATG TGTGCTCCAG TGCTTGCCTG AGGGAAGCGC CACTGTCCTT GAAGGGAGAA AACCCTCTGC AGCTGCCACT CAGATGTCAC TTCCAGAGAC GACATGCAAA GACAAACTCT CCCTCTGCTG CCCTCCATGT AAACTATAGA ACGCCCTGCG GACGGAGTCT ACGAAACATG GAGGAAGTTT TCCATTACCT GCTTGAAACG GAGTGTAACT TTTTATTCAC AGACAACTTT TCTTTTAATA CTTATGTTCA GTTAACTCGG AATCACCCAA AGCAAAATGA AGTTGTTTCT GATGTGGATA TTAGTAATGG AGTGGAATCA GTGTCGATTT CTTTCTGTAA TGAGGTTGAC AATAGTAAAC TCCCACAGTT TAAGTATAGG ACGACAGTAT GGCCCCGAGC ATATCATCTG AACGTTTCCA GCATGTTTTC TGATTCATGT GACTGTTCTG AGGGCTGCAT AGACATAAAA AAGTGTGCAT GTCTTCAGTT GACAGCAAAG AATGCCAAAG CATGTCCCTT GTCACCTGAC GGAGAATGTA CTGGATATAA ATATAAAAGA CTGCAGAGAC TCATACCTTC TGGCATTTAT GAATGCAACC TGTTCTGCAA GTGTAACCGA CAGATGTGTC AAAACCGCGT CATCCAGCAT GGCCCCCGGG TGAGGCTGCA GGTGTTCAAA AGTGAGAAGA AGGGCTGGGG AGTGCGCTGC CTGGATGACA TTGACAAAGG GACATTTGTG TGCATTTATT CAGGAAGGTT ACTGAGCAGA GCCACTCCTG AGAAAACTAA CATTGGTGAA AGTGAAAGTG AGCAGCAGCA CACCGTGAAA AATTCATTCT CTAAAAAGAG GAAAATAGAA GTTGTATGTT CAGATCGTCA AACACACTGT GAAAGTCCTA AAACTGAGGG ATGTCCTCCT AAGTTTAGCA GTGATCTCGA AGAGCCCGTT GTGGAAATGA ATAATAGAAA TATTTCAAGA ACTCAGCGTC ATTCAGTCAT TAGAAGACCT AAATCCAAGA CAGATGTTTT TCATTACAAT GAGAAAAACA TGGGATTCGT TTGCTCATAT TCTGCTGCCC CAGAAGAGAA GAATGGATTT AAACCAGCTC AAGAACACCT GGACTCTAAA GCTAGGAGAG CTCACGAGGA CTTAAGTTCA AGCCAAGTTG GATATTCTGA AGACAAGCAG CTGACTGAAT CAGATGTGAT AGATACAACT ACCAGTAGAG AAGACACTTC CCCAGCATAC GGGTGTAAGC ACGCAGCCAT ATTGAATAGT AAGAACACTA AAAAAGTGCT TGAAGTCCCC GTAAAAACGT CCCAAGAGGA AGAGCCTGCA GCCTCTCAAA GCCAGCAGGT CTCGTGTGGT GAGGAGCTGG CAAGTGAAAG GACAAAGATT CCATCTGCTT CCCTGGTGCA GCTCAGTAAG GAGAGTCTGT TTCTATTGGA TGCTTCAAAA GAAGGAAATG TGGGCCGTTT CCTTAATCAC AGTTGCTACC CAAATCTCTG GGTGCAGAAC GTGTTTGTAG AAACCCATGA CAGGAATTTC CCATTGGCGG CCTTCTTCAC CAACAGGTAT GTGAAAGCAA GAACAGAACT AACATGGGAT TATGGTTATG AAGCTGGGAC CATGCCTGAA AAGGAAATCC TCTGCCAATG TTTCTGTCAG GTTACAGAGA AGATGGAGAA GAGGACATGT GTCCTCTGCC CTGAAGGCCA CGACTGGAGT GTGATTTACT TTTCACCATC AGCAAATATA GCTGCTCATG AGAACTGTCT GCTGTATTCA TCAGGACTGG TGGAGTGTGA GGCCCATGAT CCATTTAATA TAGCTAAAAA TTTTGATGTA AGTTCAGTAC TTGAGAAGAT CTGGAACGGA AGCACGTCGA AATGCTCATT TTGTAATAAC GAGGGAGCCA TCATGGGGTG TGATGAAACT TCCTGTGCCA AGAATTACCA CTTACTCTGT GCCAAGGAGG ACCGCGCAAT TCTACAAGTT GGAGTCAAAA GAACTTACAA AATATTTTGC CCAGAACATC CTCCACAACA AGAAGAGACC ACTGAACGTG CCAATGGCCT AAGCATCAAG AAGAGGAGAG GAAGGAAGAA ACGACGTTCA TTGGGCCCTC CCGCACAGCC AAAAACGATG AAATGCAGGA GATCTAGAAG ACATGTGACA GGAGAGGCTC TCGGCCAGAG AGATGCAGCT GTCAAAGCTC CTTTTCTTAA GAAATGCCTG GAAGCAGGAC TTCTTACTGA ATTATTTGAA CAGATACAAC AAAAAATGAT TTCAATTCAT GGAAGATTCA TGGATGAGAC TGCTTCAGAG TCAGACTATG AAGGGATCGA GACCTTAGTG TTTGGCTGTG GACTATTTAA AGACACATTA ATAAAATTAG AAGAAGCAAT CAAGAGTAAA ACTTGTGAAT ATGAAGAAAG GCTGAACCAG ATGAAGCAGA AGCTTGAGGC ACTCGCACAC TTACAACAAA ACCTGCACTC ATTTCAAGAA TGTGGGGACC TGGACCCCTC AGGCTCTACC TCAGGGTCCT TGCTACCTCC TGAGGACCAC CAGTGCAGAT GCCAGGAGTC TCCTGAAGTG CAAGCAGGCT CAGGAGACAG CCTGTGACTC GAGCTCAAGC TTCGAATTCT GCAGTCGACG GTACCGCGGG CCCGGGATCG ATCCGCCCCT CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA AGCTTCTTGA AGACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGTATAA GATACACCTG CAAAGGCGGC ACAACCCCAG TGCCACGTTG TGAGTTGGAT AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC AAGCGTATTC AACAAGGGGC TGAAGGATGC CCAGAAGGTA CCCCATTGTA TGGGATCTGA TCTGGGGCCT CGGTACACAT GCTTTACATG TGTTTAGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCTAGGAGA CGACCTTCCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC CGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC GACCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCGGGAT CCACCGGATC TAGATAACTG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAACG CGATCAAGCT CCAATTCGCC CTATAGTGAG TCGTATTACG CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGCTAG TGGATCCCCC GGGCTGCAGG AATTCGATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC G
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