pLV3-CMV-ALK(human)-3×FLAG-EF1a-CopGFP-Puro
$113.00
In stock
SKU
PL50609
100μg liquid plasmid(Instantly):$134
| name | pLV3-CMV-ALK(human)-3×FLAG-EF1a-CopGFP-Puro | another name | apLV3-CMV-ALK(human)-3×FLAG-EF1a-CopGFP-Puro |
| Insert Size (bp) | 4860bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT AGAGCTAGCG AATTCGCCAC CATGGGAGCC ATCGGGCTCC TGTGGCTGCT GCCGCTGCTG CTTTCCACGG CAGCTGTGGG CTCCGGGATG GGGACCGGCC AGCGCGCGGG CTCCCCAGCT GCGGGGCCGC CGCTGCAGCC CCGGGAGCCA CTCAGCTACT CGCGCCTGCA GAGGAAGAGT CTGGCAGTTG ACTTCGTGGT GCCCTCGCTC TTCCGTGTCT ACGCCCGGGA CCTACTGCTG CCACCATCCT CCTCGGAGCT GAAGGCTGGC AGGCCCGAGG CCCGCGGCTC GCTAGCTCTG GACTGCGCCC CGCTGCTCAG GTTGCTGGGG CCGGCGCCGG GGGTCTCCTG GACCGCCGGT TCACCAGCCC CGGCAGAGGC CCGGACGCTG TCCAGGGTGC TGAAGGGCGG CTCCGTGCGC AAGCTCCGGC GTGCCAAGCA GTTGGTGCTG GAGCTGGGCG AGGAGGCGAT CTTGGAGGGT TGCGTCGGGC CCCCCGGGGA GGCGGCTGTG GGGCTGCTCC AGTTCAATCT CAGCGAGCTG TTCAGTTGGT GGATTCGCCA AGGCGAAGGG CGACTGAGGA TCCGCCTGAT GCCCGAGAAG AAGGCGTCGG AAGTGGGCAG AGAGGGAAGG CTGTCCGCGG CAATTCGCGC CTCCCAGCCC CGCCTTCTCT TCCAGATCTT CGGGACTGGT CATAGCTCCT TGGAATCACC AACAAACATG CCATCTCCTT CTCCTGATTA TTTTACATGG AATCTCACCT GGATAATGAA AGACTCCTTC CCTTTCCTGT CTCATCGCAG CCGATATGGT CTGGAGTGCA GCTTTGACTT CCCCTGTGAG CTGGAGTATT CCCCTCCACT GCATGACCTC AGGAACCAGA GCTGGTCCTG GCGCCGCATC CCCTCCGAGG AGGCCTCCCA GATGGACTTG CTGGATGGGC CTGGGGCAGA GCGTTCTAAG GAGATGCCCA GAGGCTCCTT TCTCCTTCTC AACACCTCAG CTGACTCCAA GCACACCATC CTGAGTCCGT GGATGAGGAG CAGCAGTGAG CACTGCACAC TGGCCGTCTC GGTGCACAGG CACCTGCAGC CCTCTGGAAG GTACATTGCC CAGCTGCTGC CCCACAACGA GGCTGCAAGA GAGATCCTCC TGATGCCCAC TCCAGGGAAG CATGGTTGGA CAGTGCTCCA GGGAAGAATC GGGCGTCCAG ACAACCCATT TCGAGTGGCC CTGGAATACA TCTCCAGTGG AAACCGCAGC TTGTCTGCAG TGGACTTCTT TGCCCTGAAG AACTGCAGTG AAGGAACATC CCCAGGCTCC AAGATGGCCC TGCAGAGCTC CTTCACTTGT TGGAATGGGA CAGTCCTCCA GCTTGGGCAG GCCTGTGACT TCCACCAGGA CTGTGCCCAG GGAGAAGATG AGAGCCAGAT GTGCCGGAAA CTGCCTGTGG GTTTTTACTG CAACTTTGAA GATGGCTTCT GTGGCTGGAC CCAAGGCACA CTGTCACCCC ACACTCCTCA GTGGCAGGTC AGGACCCTAA AGGATGCCCG GTTCCAGGAC CACCAAGACC ATGCTCTATT GCTCAGTACC ACTGATGTCC CCGCTTCTGA AAGTGCTACA GTGACCAGTG CTACGTTTCC TGCACCGATC AAGAGCTCTC CATGTGAGCT CCGAATGTCC TGGCTCATTC GTGGAGTCTT GAGGGGAAAC GTGTCCTTGG TGCTAGTGGA GAACAAAACC GGGAAGGAGC AAGGCAGGAT GGTCTGGCAT GTCGCCGCCT ATGAAGGCTT GAGCCTGTGG CAGTGGATGG TGTTGCCTCT CCTCGATGTG TCTGACAGGT TCTGGCTGCA GATGGTCGCA TGGTGGGGAC AAGGATCCAG AGCCATCGTG GCTTTTGACA ATATCTCCAT CAGCCTGGAC TGCTACCTCA CCATTAGCGG AGAGGACAAG ATCCTGCAGA ATACAGCACC CAAATCAAGA AACCTGTTTG AGAGAAACCC AAACAAGGAG CTGAAACCCG GGGAAAATTC ACCAAGACAG ACCCCCATCT TTGACCCTAC AGTTCATTGG CTGTTCACCA CATGTGGGGC CAGCGGGCCC CATGGCCCCA CCCAGGCACA GTGCAACAAC GCCTACCAGA ACTCCAACCT GAGCGTGGAG GTGGGGAGCG AGGGCCCCCT GAAAGGCATC CAGATCTGGA AGGTGCCAGC CACCGACACC TACAGCATCT CGGGCTACGG AGCTGCTGGC GGGAAAGGCG GGAAGAACAC CATGATGCGG TCCCACGGCG TGTCTGTGCT GGGCATCTTC AACCTGGAGA AGGATGACAT GCTGTACATC CTGGTTGGGC AGCAGGGAGA GGACGCCTGC CCCAGTACAA ACCAGTTAAT CCAGAAAGTC TGCATTGGAG AGAACAATGT GATAGAAGAA GAAATCCGTG TGAACAGAAG CGTGCATGAG TGGGCAGGAG GCGGAGGAGG AGGGGGTGGA GCCACCTACG TATTTAAGAT GAAGGATGGA GTGCCGGTGC CCCTGATCAT TGCAGCCGGA GGTGGTGGCA GGGCCTACGG GGCCAAGACA GACACGTTCC ACCCAGAGAG ACTGGAGAAT AACTCCTCGG TTCTAGGGCT AAACGGCAAT TCCGGAGCCG CAGGTGGTGG AGGTGGCTGG AATGATAACA CTTCCTTGCT CTGGGCCGGA AAATCTTTGC AGGAGGGTGC CACCGGAGGA CATTCCTGCC CCCAGGCCAT GAAGAAGTGG GGGTGGGAGA CAAGAGGGGG TTTCGGAGGG GGTGGAGGGG GGTGCTCCTC AGGTGGAGGA GGCGGAGGAT ATATAGGCGG CAATGCAGCC TCAAACAATG ACCCCGAAAT GGATGGGGAA GATGGGGTTT CCTTCATCAG TCCACTGGGC ATCCTGTACA CCCCAGCTTT AAAAGTGATG GAAGGCCACG GGGAAGTGAA TATTAAGCAT TATCTAAACT GCAGTCACTG TGAGGTAGAC GAATGTCACA TGGACCCTGA AAGCCACAAG GTCATCTGCT TCTGTGACCA CGGGACGGTG CTGGCTGAGG ATGGCGTCTC CTGCATTGTG TCACCCACCC CGGAGCCACA CCTGCCACTC TCGCTGATCC TCTCTGTGGT GACCTCTGCC CTCGTGGCCG CCCTGGTCCT GGCTTTCTCC GGCATCATGA TTGTGTACCG CCGGAAGCAC CAGGAGCTGC AAGCCATGCA GATGGAGCTG CAGAGCCCTG AGTACAAGCT GAGCAAGCTC CGCACCTCGA CCATCATGAC CGACTACAAC CCCAACTACT GCTTTGCTGG CAAGACCTCC TCCATCAGTG ACCTGAAGGA GGTGCCGCGG AAAAACATCA CCCTCATTCG GGGTCTGGGC CATGGCGCCT TTGGGGAGGT GTATGAAGGC CAGGTGTCCG GAATGCCCAA CGACCCAAGC CCCCTGCAAG TGGCTGTGAA GACGCTGCCT GAAGTGTGCT CTGAACAGGA CGAACTGGAT TTCCTCATGG AAGCCCTGAT CATCAGCAAA TTCAACCACC AGAACATTGT TCGCTGCATT GGGGTGAGCC TGCAATCCCT GCCCCGGTTC ATCCTGCTGG AGCTCATGGC GGGGGGAGAC CTCAAGTCCT TCCTCCGAGA GACCCGCCCT CGCCCGAGCC AGCCCTCCTC CCTGGCCATG CTGGACCTTC TGCACGTGGC TCGGGACATT GCCTGTGGCT GTCAGTATTT GGAGGAAAAC CACTTCATCC ACCGAGACAT TGCTGCCAGA AACTGCCTCT TGACCTGTCC AGGCCCTGGA AGAGTGGCCA AGATTGGAGA CTTCGGGATG GCCCGAGACA TCTACAGGGC GAGCTACTAT AGAAAGGGAG GCTGTGCCAT GCTGCCAGTT AAGTGGATGC CCCCAGAGGC CTTCATGGAA GGAATATTCA CTTCTAAAAC AGACACATGG TCCTTTGGAG TGCTGCTATG GGAAATCTTT TCTCTTGGAT ATATGCCATA CCCCAGCAAA AGCAACCAGG AAGTTCTGGA GTTTGTCACC AGTGGAGGCC GGATGGACCC ACCCAAGAAC TGCCCTGGGC CTGTATACCG GATAATGACT CAGTGCTGGC AACATCAGCC TGAAGACAGG CCCAACTTTG CCATCATTTT GGAGAGGATT GAATACTGCA CCCAGGACCC GGATGTAATC AACACCGCTT TGCCGATAGA ATATGGTCCA CTTGTGGAAG AGGAAGAGAA AGTGCCTGTG AGGCCCAAGG ACCCTGAGGG GGTTCCTCCT CTCCTGGTCT CTCAACAGGC AAAACGGGAG GAGGAGCGCA GCCCAGCTGC CCCACCACCT CTGCCTACCA CCTCCTCTGG CAAGGCTGCA AAGAAACCCA CAGCTGCAGA GATCTCTGTT CGAGTCCCTA GAGGGCCGGC CGTGGAAGGG GGACACGTGA ATATGGCATT CTCTCAGTCC AACCCTCCTT CGGAGTTGCA CAAGGTCCAC GGATCCAGAA ACAAGCCCAC CAGCTTGTGG AACCCAACGT ACGGCTCCTG GTTTACAGAG AAACCCACCA AAAAGAATAA TCCTATAGCA AAGAAGGAGC CACACGACAG GGGTAACCTG GGGCTGGAGG GAAGCTGTAC TGTCCCACCT AACGTTGCAA CTGGGAGACT TCCGGGGGCC TCACTGCTCC TAGAGCCCTC TTCGCTGACT GCCAATATGA AGGAGGTACC TCTGTTCAGG CTACGTCACT TCCCTTGTGG GAATGTCAAT TACGGCTACC AGCAACAGGG CTTGCCCTTA GAAGCCGCTA CTGCCCCTGG AGCTGGTCAT TACGAGGATA CCATTCTGAA AAGCAAGAAT AGCATGAACC AGCCTGGGCC CGGATCCGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGTGAG CGGCCGCGAA GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG TGACCGGCGC CTACGCTAGA CGCCACCATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCAAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGGTGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGTGCTGGT GGGCAGCTTC GCCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT TCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGCTGCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC CCATCGCCTT CGCCAGATCC CGCGCTCAGT CGTCCAATTC TGCCGTGGAC GGCACCGCCG GACCCGGCTC CACCGGATCT CGCGAGGGCA GAGGAAGTCT TCTAACATGC GGTGACGTGG AGGAGAATCC CGGCCCTATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGAAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCCTGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAAATA AGATCTGCTT TTTGCTTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGTAGTAG TTCATGTCAT CTTATTATTC AGTATTTATA ACTTGCAAAG AAATGAATAT CAGAGAGTGA GAGGAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGCTCT AGCTATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGACTTTTG CAGAGACGGC CCAAATTCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCAAG CTG
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