pUT-mini-Tn5-Pc-Gen-Tac-mCherry-SacB
$142.00
In stock
SKU
PL50427
100μg liquid plasmid(Instantly):$163
| name | pUT-mini-Tn5-Pc-Gen-Tac-mCherry-SacB | another name | apUT-mini-Tn5-Pc-Gen-Tac-mCherry-SacB |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | Red | |
| Promoter | Tac | Replicon | R6K |
| Competence | S17-1λpir | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCCTTTTT GTCCGGTGTT GGGTTGAAGG TGAAGCCGGT CGGGGCCGCA GCGGGGGCCG GCTTTTCAGC CTTGCCCCCC TGCTTCGGCC GCCGTGGCTC CGGCGTCTTG GGTGCCGGCG CGGGTTCCGC AGCCTTGGCC TGCGGTGCGG GCACATCGGC GGGCTTGGCC TTGATGTGCC GCCTGGCGTG CGAGCGGAAC GTCTCGTAGG AGAACTTGAC CTTCCCCGTT TCCCGCATGT GCTCCCAAAT GGTGACGAGC GCATAGCCGG ACGCTAACGC CGCCTCGACA TCCGCCCTCA CCGCCAGGAA CGCAACCGCA GCCTCATCAC GCCGGCGCTT CTTGGCCGCG CGGGATTCAA CCCACTCGGC CAGCTCGTCG GTGTAGCTCT TTGGCATCGT CTCTCGCCTG TCCCCTCAGT TCAGTAATTT CCTGCATTTG CCTGTTTCCA GTCGGTAGAT ATTCCACAAA ACAGCAGGGA AGCAGCGCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GGCTACCGCC GGCGTAACAG ATGAGGGCAA GCGGATGGCT GATGAAACCA AGCCAACCAG GAAGGGCAGC CCACCTATCA AGGTGTACTG CCTTCCAGAC GAACGAAGAG CGATTGAGGA AAAGGCGGCG GCGGCCGGCA TGAGCCTGTC GGCCTACCTG CTGGCCGTCG GCCAGGGCTA CAAAATCACG GGCGTCGTGG ACTATGAGCA CGTCCGCGAG CTGGCCCGCA TCAATGGCGA CCTGGGCCGC CTGGGCGGCC TGCTGAAACT CTGGCTCACC GACGACCCGC GCACGGCGCG GTTCGGTGAT GCCACGATCC TCGCCCTGCT GGCGAAGATC GAAGAGAAGC AGGACGAGCT TGGCAAGGTC ATGATGGGCG TGGTCCGCCC GAGGGCAGAG CCATGACTTT TTTAGCCGCT AAAACGGCCG GGGGGTGCGC GTGATTGCCA AGCACGTCCC CATGCGCTCC ATCAAGAAGA GCGACTTCGC GGAGCTGGTG AAGTACATCA CCGACGAGCA AGGCAAGACC GAGCGCCTGG GTCACGTGCG CGTCACGAAC TGCGAGGCAA ACACCCTGCC CGCTGTCATG GCCGAGGTGA TGGCGACCCA GCACGGCAAC ACCCGTTCCG AGGCCGACAA GACCTATCAC CTGCTGGTTA GCTTCCGCGC GGGAGAGAAG CCCGACGCGG AGACGTTGCG CGCGATTGAG GACCGCATCT GCGCTGGGCT TGGCTTCGCC GAGCATCAGC GCGTCAGTGC CGTGCATCAC GACACCGACA ACCTGCACAT CCATATCGCC ATCAACAAGA TTCACCCGAC CCGAAACACC ATCCATGAGC CGTATCGGGC CTACCGCGCC CTCGCTGACC TCTGCGCGAC GCTCGAACGG GACTACGGGC TTGAGCGTGA CAATCACGAA ACGCGGCAGC GCGTTTCCGA GAACCGCGCG AACGACATGG AGCGGCACGC GGGCGTGGAA AGCCTGGTCG GCTGGATCCG GCCACGATGC GTCCGGCGTA GAGGATCTGA AGATCAGCAG TTCAACCTGT TGATAGTACG TACTAAGCTC TCATGTTTCA CGTACTAAGC TCTCATGTTT AACGTACTAA GCTCTCATGT TTAACGAACT AAACCCTCAT GGCTAACGTA CTAAGCTCTC ATGGCTAACG TACTAAGCTC TCATGTTTCA CGTACTAAGC TCTCATGTTT GAACAATAAA ATTAATATAA ATCAGCAACT TAAATAGCCT CTAAGGTTTT AAGTTTTATA AGAAAAAAAA GAATATATAA GGCTTTTAAA GCTTTTAAGG TTTAACGGTT GTGGACAACA AGCCAGGGAT GTAACGCACT GAGAAGCCCT TAGAGCCTCT CAAAGCAATT TTGAGTGACA CAGGAACACT TAACGGCTGA CATGGGAATT CCACATGTGG AATTCCACAT GTGGAATTGT GAGCGGATAA CAATTTGTGG AATTCCCGGG AGAGCTCCTG ACTCTTATAC ACAAGTTCGG CCACGGCCTA GGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCCAGG GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT TGCCCTTCAC CGCCTGGCCC TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG ATGGTGGTTA ACGGCGGGAT ATAACATGAG CTGTCTTCGG TATCGTCGTA TCCCACTACC GAGATATCCG CACCAACGCG CAGCCCGGAC TCGGTAATGG CGCGCATTGC GCCCAGCGCC ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC CCTCATTCAG CATTTGCATG GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC GTTCCGCTAT CGGCTGAATT TGATTGCGAG TGAGATATTT ATGCCAGCCA GCCAGACGCA GACGCGCCGA GACAGAACTT AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG CGACCAGATG CTCCACGCCC AGTCGCGTAC CGTCTTCATG GGAGAAAATA ATACTGTTGA TGGGTGTCTG GTCAGAGACA TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA CAGCAATGGC ATCCTGGTCA TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACGCGTTGCG CGAGAAGATT GTGCACCGCC GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT GGCACCCAGT TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG GCGCGTGCAG GGCCAGACTG GAGGTGGCAA CGCCAATCAG CAACGACTGT TTGCCCGCCA GTTGTTGTGC CACGCGGTTG GGAATGTAAT TCAGCTCCGC CATCGCCGCT TCCACTTTTT CCCGCGTTTT CGCAGAAACG TGGCTGGCCT GGTTCACCAC GCGGGAAACG GTCTGATAAG AGACACCGGC ATACTCTGCG ACATCGTATA ACGTTACTGG TTTCACATTC ACCACCCTGA ATTGACTCTC TTCCGGGCGC TATCATGCCA TACCGCGAAA GGTTTTGCGC CATTCGATGG TGTCCGGGAT CTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAATT TGACAATTAA TCATCGGCTC GTATAATGTG TGGAATGTCT CATGAGCGGA TATTTCACAC AGGAAACAGT ATTCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAA CGTCAACATC AAGTTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA AGTAATTAAC CTAGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGGCGG CCGCAGCTTG TCGACGAATT CCCGGGGATC CGGTGATTGA TTGAGCAAGC TTTATGCTTG TAAACCGTTT TGTGAAAAAA TTTTTAAAAT AAAAAAGGGG ACCTCTAGGG TCCCCAATTA ATTAGTAATA TAATCTATTA AAGGTCATTC AAAAGGTCAT CCACCGGATC AATTCCCCTG CTCACACAGA CTGGGTACCA AGCTCTCGGT AACATCAAGG CCCGATCCTT GGAGCCCTTG CCCTCCCGAC GATGATCGTG CCGTGATCGA AATCCAGATC CTTGACCCAC AGTTGCAAAC CCTCACTGAT CCGTCCCGTC CGGGTCGACC AAAGCGGCCA TCGTGCCTCC CCACTCCTGC AGTTCGGGGG CATGGATGCG CGGATAGCCG CTGCTGGTTT CCTGGATGCC GACGGATTTG CACTGCCGGT AGAACTCCGC GAGGTCGTCC AGCCTCAGGC AGCAGCTGAA CCAACTCGCG AGGGGATCGA GCCCGGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTTA GGTGGCGGTA CTTGGGTCGA TATCAAAGTG CATCACTTCT TCCCGTATGC CCAACTTTGT ATAGAGAGCC ACTGCGGGAT CGTCACCGTA ATCTGCTTGC ACGTAGATCA CATAAGCACC AAGCGCGTTG GCCTCATGCT TGAGGAGATT GATGAGCGCG GTGGCAATGC CCTGCCTCCG GTGCTCGCCG GAGACTGCGA GATCATAGAT ATAGATCTCA CTACGCGGCT GCTCAAACCT GGGCAGAACG TAAGCCGCGA GAGCGCCAAC AACCGCTTCT TGGTCGAAGG CAGCAAGCGC GATGAATGTC TTACTACGGA GCAAGTTCCC GAGGTAATCG GAGTCCGGCT GATGTTGGGA GTAGGTGGCT ACGTCTCCGA ACTCACGACC GAAAAGATCA AGAGCAGCCC GCATGGATTT GACTTGGTCA GGGCCGAGCC TACATGTGCG AATGATGCCC ATACTTGAGC CACCTAACTT TGTTTTAGGG CGACTGCCCT GCTGCGTAAC ATCGTTGCTG CTGCGTAACA TCGTTGCTGC TCCATAACAT CAAACATCGA CCCACGGCGT AACGCGCTTG CTGCTTGGAT GCCCGAGGCA TAGACTGTAC AAAAAAACAG TCATAACAAG CCATGAAAAC CGCCACTGCG CCGTTACCAC CGCTGCGTTC GGTCAAGGTT CTGGACCAGT TGCGTGAGCG CATACGCTAC TTGCATTACA GTTTACGAAC CGAACAGGCT TATGTCAAGC GAAACGATCC TCATCCTGTC TCTTGATCAG ATCTTGATCC CCTGCGCCAT CAGATCCTTG GCGGCAAGAA AGCCATCCAG TTTACTTTGC AGGGCTTCCC AACCTTACCA GAGGGCGCCC CAGCTGGCAA TTCCGGTTCG CTTGCTGTCC ATAAAACCGC CCAGTCTAGC TATCGTCATG TAAGCCCACT GCAAGCTACC TCTTTCTCTT TGCGCTTGCG TTTTCCCTTG TCCAGATAGC CCAGTAGCTG ACATTCATCC GGGGTAGCAC CGTTTCTGCG GACTGGCTTT CTACGTGTTC CGCTTCCTTT AGCAGCCCTT GCGCCCTGAT GCTTGCGGCA GCGTGAAGCT CGCGCAGATC AGTTGGTTGA TTTTGTCCAC TACGTGAAAG GCGAATCACC AAGGTAGTCG GCAAATAATG TCTAACAATT CGTTCAAGCC GACGCCGCTT CGCGGCGCGG TTAACTCAAG CGTTAGATGC ACTAAGCACA TAATTGCTCA CAGCCAAACT ATCAGGTCAA GTCGCTTTTA TTATTTTTAA GCGTGCATAA TAAGCCCTAC ACAAAATTGG GAGATATATC ATGAAAGGCG CTTTTCTTGT TATCGCAATA GTTGGGCGAA GTAATCGCAA CATCCGCATT AACAATCTAG CGAGGGCTTG GTAAGGTGAT CCGGTGGATG ACCTTTTGAA TGACCTTTAA TAGATTATAT TACTAATTAA TTGGGGACCC TAGAGGTCCC CTTTTTTATT TTAAAAATTT TTTCACAAAA CGGTTTACAA GCATAAAGCT TGCTCAATCA ATCACCGGAT CCCCGGGAAT TCGGCCTAGG TGGCCAGATC TGATCAAGAG ACAGAAACGC AAAAGAAAAT GCCGATATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG GATAACAATT CCCCTCTAGA AATAATTTTG TTAACTTTAA GAAGGAGATA TACATATGAT TACCAGTGCA CTGCATCGTG CGGCGGATTG GGCGAAAAGC GTGTTTTCTA GTGCTGCGCT GGGTGATCCG CGTCGTACCG CGCGTCTGGT GAATGTTGCG GCGCAACTGG CCAAATATAG CGGCAAAAGC ATTACCATTA GCAGCGAAGG CAGCAAAGCC ATGCAGGAAG GCGCGTATCG TTTTATTCGT AATCCGAACG TGAGCGCGGA AGCGATTCGT AAAGCGGGTG CCATGCAGAC CGTGAAACTG GCCCAGGAAT TTCCGGAACT GCTGGCAATT GAAGATACCA CCTCTCTGAG CTATCGTCAT CAGGTGGCGG AAGAACTGGG CAAACTGGGT AGCATTCAGG ATAAAAGCCG TGGTTGGTGG GTGCATAGCG TGCTGCTGCT GGAAGCGACC ACCTTTCGTA CCGTGGGCCT GCTGCATCAA GAATGGTGGA TGCGTCCGGA TGATCCGGCG GATGCGGATG AAAAAGAAAG CGGCAAATGG CTGGCCGCTG CTGCAACTTC GCGTCTGAGA ATGGGCAGCA TGATGAGCAA CGTGATTGCG GTGTGCGATC GTGAAGCGGA TATTCATGCG TATCTGCAAG ATAAACTGGC CCATAACGAA CGTTTTGTGG TGCGTAGCAA ACATCCGCGT AAAGATGTGG AAAGCGGCCT GTATCTGTAT GATCACCTGA AAAACCAGCC GGAACTGGGC GGCTATCAGA TTAGCATTCC GCAGAAAGGC GTGGTGGATA AACGTGGCAA ACGTAAAAAC CGTCCGGCGC GTAAAGCGAG CCTGAGCCTG CGTAGCGGCC GTATTACCCT GAAACAGGGC AACATTACCC TGAACGCGGT GCTGGCCGAA GAAATTAATC CGCCGAAAGG CGAAACCCCG CTGAAATGGC TGCTGCTGAC CAGCGAGCCG GTGGAAAGTC TGGCCCAAGC GCTGCGTGTG ATTGATATTT ATACCCATCG TTGGCGCATT GAAGAATTTC ACAAAGCGTG GAAAACGGGT GCGGGTGCGG AACGTCAGCG TATGGAAGAA CCGGATAACC TGGAACGTAT GGTGAGCATT CTGAGCTTTG TGGCGGTGCG TCTGCTGCAA CTGCGTGAAT CTTTTACTCC GCCGCAAGCA CTGCGTGCGC AGGGCCTGCT GAAAGAAGCG GAACACGTTG AAAGCCAGAG CGCGGAAACC GTGCTGACCC CGGATGAATG CCAACTGCTG GGCTATCTGG ATAAAGGCAA ACGCAAACGC AAAGAAAAAG CGGGCAGCCT GCAATGGGCG TATATGGCGA TTGCGCGTCT GGGCGGCTTT ATGGATAGCA AACGTACCGG CATTGCGAGC TGGGGTGCGC TGTGGGAAGG TTGGGAAGCG CTGCAAAGCA AACTGGATGG CTTTCTGGCC GCGAAAGACC TGATGGCGCA GGGCATTAAA ATCTAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGACGCGT GCTATCGTCA TGTAAGCCCA CTGATCGGCA TTTTCTTTTG CGTTTTTATT TGTTAACTGT TAATTGTCCT TGTTCAAGGA TGCTGTCTTT GACAACAGAT GTTTTCTTGC CTTTGATGTT CAGCAGGAAG CTCGGCGCAA ACGTTGATTG TTTGTCTGCG TAGAATCCTC TGTTTGTCAT ATAGCTTGTA ATCACGACAT TGTTTCCTTT CGCTTGAGGT ACAGCGAAGT GTGAGTAAGT AAAGGTTACA TCGTTAGGAT CAAGATCCAT TTTTAACACA AGGCCAGTTT TGTTCAGCGG CTTGTATGGG CCAGTTAAAG AATTAGAAAC ATAACCAAGC ATGTAAATAT CGTTAGACGT AATGCCGTCA ATCGTCATTT TTGATCCGCG GGAGTCAGTG AACAGGTACC ATTTGCCGTT CATTTTAAAG ACGTTCGCGC GTTCAATTTC ATCTGTTACT GTGTTAGATG CAATCAGCGG TTTCATCACT TTTTTCAGTG TGTAATCATC GTTTAGCTCA ATCATACCGA GAGCGCCGTT TGCTAACTCA GCCGTGCGTT TTTTATCGCT TTGCAGAAGT TTTTGACTTT CTTGACGGAA GAATGATGTG CTTTTGCCAT AGTATGCTTT GTTAAATAAA GATTCTTCGC CTTGGTAGCC ATCTTCAGTT CCAGTGTTTG CTTCAAATAC TAAGTATTTG TGGCCTTTAT CTTCTACGTA GTGAGGATCT CTCAGCGTAT GGTTGTCGCC TGAGCTGTAG TTGCCTTCAT CGATGAACTG CTGTACATTT TGATACGTTT TTCCGTCACC GTCAAAGATT GATTTATAAT CCTCTACACC GTTGATGTTC AAAGAGCTGT CTGATGCTGA TACGTTAACT TGTGCAGTTG TCAGTGTTTG TTTGCCGTAA TGTTTACCGG AGAAATCAGT GTAGAATAAA CGGATTTTTC CGTCAGATGT AAATGTGGCT GAACCTGACC ATTCTTGTGT TTGGTCTTTT AGGATAGAAT CATTTGCATC GAATTTGTCG CTGTCTTTAA AGACGCGGCC AGCGTTTTTC CAGCTGTCAA TAGAAGTTTC GCCGACTTTT TGATAGAACA TGTAAATCGA TGTGTCATCC GCATTTTTAG GATCTCCGGC TAATGCAAAG ACGATGTGGT AGCCGTGATA GTTTGCGACA GTGCCGTCAG CGTTTTGTAA TGGCCAGCTG TCCCAAACGT CCAGGCCTTT TGCAGAAGAG ATATTTTTAA TTGTGGACGA ATCAAATTCA GAAACTTGAT ATTTTTCATT TTTTTGCTGT TCAGGGATTT GCAGCATATC ATGGCGTGTA ATATGGGAAA TGCCGTATGT TTCCTTATAT GGCTTTTGGT TCGTTTCTTT CGCAAACGCT TGAGTTGCGC CTCCTGCCAG CAGTGCGGTA GTAAAGGTTA ATACTGTTGC TTGTTTTGCA AACTTTTTGA TGTTCATCGT TCATGTCTCC TTTTTTATGT ACTGTGTTAG CGGTCTGCTT CTTCCAGCCC TCCTGTTTGA AGATGGCAAG TTAGTTACGC ACAATAAAAA AAGACCTAAA ATATGTAAGG GGTGACGCCA AAGTATACAC TTTGCCCTTT ACACATTTTA GGTCTTGCCT GCTTTATCAG TAACAAACCC GCGCGATTTA CTTTTCGACC TCATTCTATT AGACTCTCGT TTGGATTGCA ACTGGTCTAT TTTCCTCTTT TGTTTGATAG AAAATCATAA AAGGATTTGC AGACTACGGG CCTAAAGAAC TAAAAAATCT ATCTGTTTCT TTTCATTCTC TGTATTTTTT ATAGTTTCTG TTGCATGGGC ATAAAGTTGC CTTTTTAATC ACAATTCAGA AAATATCATA ATATCTCATT TCACTAAATA ATAGTGAACG GCAGGTATAT GTGGGTTACC GACTGCGGCC TGAGTTTTTT AAGTGACGTA AAATCGTGTT GAGGCCAACG CCCATAATGC GGGCTGTTGC CCGGCATCCA ACGCCATTCA TGGCCATATC AATGATTTTC TGGTGCGTAC CGGGTTGAGA AGCGGTGTAA GTGAACTGCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCA
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