pBAM1-EGFP
$99.00
In stock
SKU
PL50425
100μg liquid plasmid(Instantly):$142
| name | pBAM1-EGFP | another name | apBAM1-EGFP |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | Replicon | R6K | |
| Competence | S17-1λpir | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCATGTCAGC CGTTAAGTGT TCCTGTGTCA CTCAAAATTG CTTTGAGAGG CTCTAAGGGC TTCTCAGTGC GTTACATCCC TGGCTTGTTG TCCACAACCG TTAAACCTTA AAGGCTTTAA AAGCCTTATA TATTCTTTTT TTTCTTATAA AACTTAAAAC CTTAGAGGCT ATTTAAGTTG CTGATTTATA TTAATTTTAT TGTTCAAACA TGAGAGCTTA GTACGTGAAA CATGAGAGCT TAGTACGTTA GCCATGAGAG CTTAGTACGT TAGCCATGAG GGTTTAGTTC GTTAAACATG AGAGCTTAGT ACGTTAAACA TGAGAGCTTA GTACGTGAAA CATGAGAGCT TAGTACGTAC TATCAACAGG TTGAACTGCT GATCTTCAGA TCGGCGCGCC GGCCGGCCTA CGGCCAGCCT CGCAGAGCAG GATTCCCGTT GAGCACCGCC AGGTGCGAAT AAGGGACAGT GAAGAAGGAA CACCCGCTCG CGGGTGGGCC TACTTCACCT ATCCTGCCCG GCTGACGCCG TTGGATACAC CAAGGAAAGT CTACACGAAC CCTTTGGCAA AATCCTGTAT ATCGTGCGAA AAAGGATGGA TATACCGAAA AAATCGCTAT AATGACCCCG AAGCAGGGTT ATGCAGCGGA AAAGGACAAG GGTCGTCCAA AAAAAAAGGC TCCAAAAGGA GCCTTTAATT GTATCGGTTT ACCAATGTTT AATCAGGCTC GCGCCAATTT CCGCAATCTG ACGGTTACGT TCATCCATCG TCGCCTGGCT GCCGGTGGTA TAAATCACCA CAATACGGCT CGGTTTACCA TCCGGGCCCA GCGCCGCAAT AATGCCACGG CTGCCACGTT CGCCCGCACC GCTTTTATCC GCAATAAACC AGCCAGCCGG CAGCGCGCTA CGCAGCAGCG GACCCGCCAC TTTATCCGCT TCCATCCAAT CAATCAGTTG CTGGCGGCTT GCCAGGGTCA GCAGCTCACC CGTCAGCAGT TTACGCAGGG TGGTCGCCAT TGCTGCCGGC ATGGTGGTAT CACGTTCATC GTTCGGAATC GCTTCGTTCA GTTCCGGTTC CCAACGATCC AGACGGGTCA CATGATCGCC CATGTTATGC AGAAACGCGG TCAGTTCTTT CGGACCGCCA ATGGTCGTCA GCAGCAGGTT CGCCGCGGTG TTATCGCTCA TGGTAATCGC CGCGCTGCAC AGTTCACGCA CGGTCATGCC ATCGGTCAGA TGTTTTTCGG TCACCGGGCT ATATTCCACC AGATCGTTCT GGCTATAATG AATACGACGG CCCAGTTGTT CCTGGCCCGC ATCCACACGG CTCAGAACCG CACCGCACAG CAGCACTTTA AAGGTGCTCA TCATCGGAAA ACGTTCTTCC GGACGAAAAG ATTCCAGAAT TTTGCCGCTG TTCAGATCCA GTTCAATATA GCCCACGCGC GCACCCAGTT GATCTTCCGC ATCTTTCACT TTCACCAGGG TTTCCGGATG CGCAAACACC GGCAGGCAAA ACGCCGCAAA AAACGGAATC AGCGCCACAC GAAAATGCTG AATGCTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGAAT TAAAAAAAAG CCCGCTCATT AGGCGGGCTA CTAATTTAAA TCGATTAGAT TTTAATGCCC TGCGCCATCA GGTCTTTCGC GGCCAGAAAG CCATCCAGTT TGCTTTGCAG CGCTTCCCAA CCTTCCCACA GCGCACCCCA GCTCGCAATG CCGGTACGTT TGCTATCCAT AAAGCCGCCC AGACGCGCAA TCGCCATATA CGCCCATTGC AGGCTGCCCG CTTTTTCTTT GCGTTTGCGT TTGCCTTTAT CCAGATAGCC CAGCAGTTGG CATTCATCCG GGGTCAGCAC GGTTTCCGCG CTCTGGCTTT CAACGTGTTC CGCTTCTTTC AGCAGGCCCT GCGCACGCAG TGCTTGCGGC GGAGTAAAAG ATTCACGCAG TTGCAGCAGA CGCACCGCCA CAAAGCTCAG AATGCTCACC ATACGTTCCA GGTTATCCGG TTCTTCCATA CGCTGACGTT CCGCACCCGC ACCCGTTTTC CACGCTTTGT GAAATTCTTC AATGCGCCAA CGATGGGTAT AAATATCAAT CACACGCAGC GCTTGGGCCA GACTTTCCAC CGGCTCGCTG GTCAGCAGCA GCCATTTCAG CGGGGTTTCG CCTTTCGGCG GATTAATTTC TTCGGCCAGC ACCGCGTTCA GGGTAATGTT GCCCTGTTTC AGGGTAATAC GGCCGCTACG CAGGCTCAGG CTCGCTTTAC GCGCCGGACG GTTTTTACGT TTGCCACGTT TATCCACCAC GCCTTTCTGC GGAATGCTAA TCTGATAGCC GCCCAGTTCC GGCTGGTTTT TCAGGTGATC ATACAGATAC AGGCCGCTTT CCACATCTTT ACGCGGATGT TTGCTACGCA CCACAAAACG TTCGTTATGG GCCAGTTTAT CTTGCAGATA CGCATGAATA TCCGCTTCAC GATCGCACAC CGCAATCACG TTGCTCATCA TGCTGCCCAT TCTCAGACGC GAAGTTGCAG CAGCGGCCAG CCATTTGCCG CTTTCTTTTT CATCCGCATC CGCCGGATCA TCCGGACGCA TCCACCATTC TTGATGCAGC AGGCCCACGG TACGAAAGGT GGTCGCTTCC AGCAGCAGCA CGCTATGCAC CCACCAACCA CGGCTTTTAT CCTGAATGCT ACCCAGTTTG CCCAGTTCTT CCGCCACCTG ATGACGATAG CTCAGAGAGG TGGTATCTTC AATTGCCAGC AGTTCCGGAA ATTCCTGGGC CAGTTTCACG GTCTGCATGG CACCCGCTTT ACGAATCGCT TCCGCGCTCA CGTTCGGATT ACGAATAAAA CGATACGCGC CTTCCTGCAT GGCTTTGCTG CCTTCGCTGC TAATGGTAAT GCTTTTGCCG CTATATTTGG CCAGTTGCGC CGCAACATTC ACCAGACGCG CGGTACGACG CGGATCACCC AGCGCAGCAC TAGAAAACAC GCTTTTCGCC CAATCCGCCG CACGATGCAG TGCACTGGTA ATCATGAACG TTACCATGTT AGGAGGTCAC ATGGAACATC AGATTCTGGA AAACGGGAAA GGTTCCGTTC AGGACGCTAC TTGTGTAGTT TAAACCAGCT GTCTCTTATA CACATCTGAC GTCTTGTGTC TCAAAATCTC TGATGTTAGA TTGCACAAGA TAAAAATATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGGTT TCTAAAGGTG AAGAACTGTT CACCGGTGTT GTTCCGATCC TGGTTGAACT GGACGGTGAC GTTAACGGTC ACAAATTCTC TGTTTCTGGT GAAGGTGAAG GTGACGCTAC CTACGGTAAA CTGACCCTGA AATTCATCTG CACCACCGGT AAACTGCCGG TTCCGTGGCC GACCCTGGTT ACCACCCTGA CCTACGGTGT TCAGTGCTTC TCTCGTTACC CGGACCACAT GAAACAGCAC GACTTCTTCA AATCTGCTAT GCCGGAAGGT TACGTTCAGG AACGTACCAT CTTCTTCAAA GACGACGGTA ACTACAAAAC CCGTGCTGAA GTTAAATTCG AAGGTGACAC CCTGGTTAAC CGTATCGAAC TGAAAGGTAT CGACTTCAAA GAAGACGGTA ACATCCTGGG TCACAAACTG GAATACAACT ACAACTCTCA CAACGTTTAC ATCATGGCTG ACAAACAGAA AAACGGTATC AAAGTTAACT TCAAAATCCG TCACAACATC GAAGACGGTT CTGTTCAGCT GGCTGACCAC TACCAGCAGA ACACCCCGAT CGGTGACGGT CCGGTTCTGC TGCCGGACAA CCACTACCTG TCTACCCAGT CTGCTCTGTC TAAAGACCCG AACGAAAAAC GTGACCACAT GGTTCTGCTG GAATTCGTTA CCGCTGCTGG TATCACCCTG GGTATGGACG AACTGTACAA ATAATAATTA ATTGGGGACC CTAGAGGTCC CCTTTTTTAT TTTAAAAATT TTTTCACAAA ACGGTTTACA AGCATAACTA GTGCGGCCGC AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GTACCGAGCT CGAATTCGCG CGGCCGCGGC CTAGGCGGCC TTAATTAAAG ATGTGTATAA GAGACAGCTG GGCGCGCC
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