pSYVN1(human)-Fluc-Puro
$84.00
In stock
SKU
PL50387
100μg liquid plasmid(Instantly):$105
| name | pSYVN1(human)-Fluc-Puro | another name | apSYVN1(human)-Fluc-Puro |
| Insert Size (bp) | 2001bp | Plasmid host | Mammary cell |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Fluc |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTAACTG GCCGGTACCG TGCAGTGGCA CGATCTCAGC TCACTGCAAG CTCCGCCTCC CAGGTTCACG CCATTCTCCT GCATCAGCCT CCGGAGTAGC TGGGACTACA GACGCCCGCC ACCAAGCTCG GCCAATTTTT GTGTTTTTTT ATCAGAGACG GGGTTTCACC GTGTTAGCCA CGATGGTCTC GATCTCCTCA CCTCGTGATC CAACCGCCTT GGCATCCCAA AGTGCTGGGA TTACAGGGTC AGCCACCACA CCCAGCCTGG AGTGACTTTA TTACACCTAA GTCAGAGCAT GTCACACCTC TGCTCAGCAC CCTCCAATGA CTTCTCACGT CACTCAGAGG AAAAGCCAAG GTCCCTGTGA TGGCCAATGC GATCTTGCCC TGGCCTCTTT CCTACGCACC CTTCACTAGT TCCACTATAG CCACACTCAT CTCCTTCAAC AAAGCAGCAC TGCTCCACCT TTATTGAGCT GTAACTCTTT ACCCAGACAT CCACTTGGCT CATAACCTCA CTTCCTTTAA GTCTTTGCTC AAATGTCACC TTCTCAAGGA AGCTTACCCG ATTATCCTCG CTGATACTGC AACCAGCTTC AAGTACCCCA CCACATCCTG ATCCCCTTTA TTCTGTTCTA CTTTTTTCCT ATAGCACTGA TCATCTTCCA GCGTATTAGA TTTTTCACTT ATGTCTGTGG TTTGCTGTCA CATCTACTAG GATAAGCTCC ACAAAGGTAG AGATCTTTAT TTTGTTCACT GACATCCTAA GTCCCTAGAA CAGGAGACAC TTGATCCATA TTTGTAGACT AACTGAATAA ATGACTTAAT TACCAGTTTG GATGTGGGGG CAGATAGTGA GCATGATGCC CGTTTCCGGA GCTGGGGTGC AGACAGTGTC TAGGGACACT GAACTGTTTT AAAAGCAGGA TAGATCCCGG CTGGAGACCA CACAAGGAAA TCATCAGCAC CTGGGTCAGG GGCTGGACTG GAGCAGAGGA AATCATGCAG GAAAAGTAAA GAGAAGGACA TCAGGTAAAG AGAAGAGGAC ACATGCATAG CCAGAGAGAA AAGAGGAGCA GAGGCATGTG GATCACAGAA GCTTAGGGAG GAGACTTTCA AGAAGGGGAG AGAGGTTGAG TCAAGCAAGG GCTGAAAGCC AACCATTGGA TGCAGTCACT AGAAAGTTAC AGATAGGCAA GGTGTTGTGG CTCACGCCTG TAATCCCAAC ACCTTGTGGG GCTGAGGTGG GAGGATCGCT TGAGCCCGGG AGGTCGAGGC TGCAATGAGC CCTGATGGCG CCAATGCACT CCAGCCTGGG CGACAGAGCA AGACCCTGTC GCAAAAATTA ATAAATAAAT AAATAAAAAG AAAAGGGGGA AAAAAAGTTA TACGTGGCCT TACGGGGAAG CCAACTCTGA CTGGTTATAA GCTGAAACTG TCAAGTCAAC AGGTGGCAGG GAAGATGGCT GAGACCAACA GCACAGAGAT TTAGAGGCAG ACAGACCTGG CGCCAATCCT AGGACAGGTT TTGGTAAGCC TTTGAATTTC AATTGCCCCA CGTTTCGGGG GAGGGGGTAG CACCCCCTAG CTCATAAACC TTAGTGATTG ATGATTAAAT GAGATGACGG AGGAAAACGC AAGGCACAAA GTGGATGCAT TAGCTCCATT TTGTTAATCA GCAGGCTTAG TTGGCTGCGA CCCAGACACG AACTAAAATA CAGTGCAGCC CAGGACCAGT GGGGGTCTTG CTTATGGCTC AGAGCTGAAC AACACATGGG CAGCAAAATC AGACACTGAG ATGCGGGCAG GCCTGCGACG CTGAAGTCAA TTCCTTTGAA CAAACAGAAC ACTTCCGTCC CAAGATTAGC AGGAATTAAT CTCCCAGTCT CGGGTACACC TGGTTGTCCC TCCCTGTCCT GGCGCGGCAA ACGTTCCCGG AGGCCAGCCA GGGATCACTC GCCCAAGGAC TGAGCTTTCC CTACTCTCAG CCAACTGGAG CGGGACCAGG GCCTAGGCAA AAGCTTGGCA ATCCGGTACT GTTGGTAAAG CCACCATGGA AGATGCCAAA AACATTAAGA AGGGCCCAGC GCCATTCTAC CCACTCGAAG ACGGGACCGC CGGCGAGCAG CTGCACAAAG CCATGAAGCG CTACGCCCTG GTGCCCGGCA CCATCGCCTT TACCGACGCA CATATCGAGG TGGACATTAC CTACGCCGAG TACTTCGAGA TGAGCGTTCG GCTGGCAGAA GCTATGAAGC GCTATGGGCT GAATACAAAC CATCGGATCG TGGTGTGCAG CGAGAATAGC TTGCAGTTCT TCATGCCCGT GTTGGGTGCC CTGTTCATCG GTGTGGCTGT GGCCCCAGCT AACGACATCT ACAACGAGCG CGAGCTGCTG AACAGCATGG GCATCAGCCA GCCCACCGTC GTATTCGTGA GCAAGAAAGG GCTGCAAAAG ATCCTCAACG TGCAAAAGAA GCTACCGATC ATACAAAAGA TCATCATCAT GGATAGCAAG ACCGACTACC AGGGCTTCCA AAGCATGTAC ACCTTCGTGA CTTCCCATTT GCCACCCGGC TTCAACGAGT ACGACTTCGT GCCCGAGAGC TTCGACCGGG ACAAAACCAT CGCCCTGATC ATGAACAGTA GTGGCAGTAC CGGATTGCCC AAGGGCGTAG CCCTACCGCA CCGCACCGCT TGTGTCCGAT TCAGTCATGC CCGCGACCCC ATCTTCGGCA ACCAGATCAT CCCCGACACC GCTATCCTCA GCGTGGTGCC ATTTCACCAC GGCTTCGGCA TGTTCACCAC GCTGGGCTAC TTGATCTGCG GCTTTCGGGT CGTGCTCATG TACCGCTTCG AGGAGGAGCT ATTCTTGCGC AGCTTGCAAG ACTATAAGAT TCAATCTGCC CTGCTGGTGC CCACACTATT TAGCTTCTTC GCTAAGAGCA CTCTCATCGA CAAGTACGAC CTAAGCAACT TGCACGAGAT CGCCAGCGGC GGGGCGCCGC TCAGCAAGGA GGTAGGTGAG GCCGTGGCCA AACGCTTCCA CCTACCAGGC ATCCGCCAGG GCTACGGCCT GACAGAAACA ACCAGCGCCA TTCTGATCAC CCCCGAAGGG GACGACAAGC CTGGCGCAGT AGGCAAGGTG GTGCCCTTCT TCGAGGCTAA GGTGGTGGAC TTGGACACCG GTAAGACACT GGGTGTGAAC CAGCGCGGCG AGCTGTGCGT CCGTGGCCCC ATGATCATGA GCGGCTACGT TAACAACCCC GAGGCTACAA ACGCTCTCAT CGACAAGGAC GGCTGGCTGC ACAGCGGCGA CATCGCCTAC TGGGACGAGG ACGAGCACTT CTTCATCGTG GACCGGCTGA AGAGCCTGAT CAAATACAAG GGCTACCAGG TAGCCCCAGC CGAACTGGAG AGCATCCTGC TGCAACACCC CAACATCTTC GACGCCGGGG TCGCCGGCCT GCCCGACGAC GATGCCGGCG AGCTGCCCGC CGCAGTCGTC GTGCTGGAAC ACGGTAAAAC CATGACCGAG AAGGAGATCG TGGACTATGT GGCCAGCCAG GTTACAACCG CCAAGAAGCT GCGCGGTGGT GTTGTGTTCG TGGACGAGGT GCCTAAAGGA CTGACCGGCA AGTTGGACGC CCGCAAGATC CGCGAGATTC TCATTAAGGC CAAGAAGGGC GGCAAGATCG CCGTGTAATA ATTCTAGAGT CGGGGCGGCC GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG ATAAGGATCC GTTTGCGTAT TGGGCGCTCT TCCGCTGATC TGCGCAGCAC CATGGCCTGA AATAACCTCT GAAAGAGGAA CTTGGTTAGC TACCTTCTGA GGCGGAAAGA ACCAGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC GATTCTTCTG ACACTAGCGC CACCATGACC GAGTACAAGC CTACCGTGCG CCTGGCCACT CGCGATGATG TGCCCCGCGC CGTCCGCACT CTGGCCGCCG CTTTCGCCGA CTACCCCGCT ACCCGGCACA CCGTGGACCC CGACCGGCAC ATCGAGCGTG TGACAGAGTT GCAGGAGCTG TTCCTGACCC GCGTCGGGCT GGACATCGGC AAGGTGTGGG TAGCCGACGA CGGCGCGGCC GTGGCCGTGT GGACTACCCC CGAGAGCGTT GAGGCCGGCG CCGTGTTCGC CGAGATCGGC CCCCGAATGG CCGAGCTGAG CGGCAGCCGC CTGGCCGCCC AGCAGCAAAT GGAGGGCCTG CTTGCCCCCC ATCGTCCCAA GGAGCCTGCC TGGTTTCTGG CCACTGTAGG AGTGAGCCCC GACCACCAGG GCAAGGGCTT GGGCAGCGCC GTCGTGTTGC CCGGCGTAGA GGCCGCCGAA CGCGCCGGTG TGCCCGCCTT TCTCGAAACA AGCGCACCAA GAAACCTTCC ATTCTACGAG CGCCTGGGCT TCACCGTGAC CGCCGATGTC GAGGTGCCCG AGGGACCTAG GACCTGGTGT ATGACACGAA AACCTGGCGC CTAATGATCT AGAACCGGTC ATGGCCGCAA TAAAATATCT TTATTTTCAT TACATCTGTG TGTTGGTTTT TTGTGTGTTC GAACTAGATG CTGTCGACCG ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGCGGCCGCA AATGCTAAAC CACTGCAGTG GTTACCAGTG CTTGATCAGT GAGGCACCGA TCTCAGCGAT CTGCCTATTT CGTTCGTCCA TAGTGGCCTG ACTCCCCGTC GTGTAGATCA CTACGATTCG TGAGGGCTTA CCATCAGGCC CCAGCGCAGC AATGATGCCG CGAGAGCCGC GTTCACCGGC CCCCGATTTG TCAGCAATGA ACCAGCCAGC AGGGAGGGCC GAGCGAAGAA GTGGTCCTGC TACTTTGTCC GCCTCCATCC AGTCTATGAG CTGCTGTCGT GATGCTAGAG TAAGAAGTTC GCCAGTGAGT AGTTTCCGAA GAGTTGTGGC CATTGCTACT GGCATCGTGG TATCACGCTC GTCGTTCGGT ATGGCTTCGT TCAACTCTGG TTCCCAGCGG TCAAGCCGGG TCACATGATC ACCCATATTA TGAAGAAATG CAGTCAGCTC CTTAGGGCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCG GTGTTGTCGC TCATGGTAAT GGCAGCACTA CACAATTCTC TTACCGTCAT GCCATCCGTA AGATGCTTTT CCGTGACCGG CGAGTACTCA ACCAAGTCGT TTTGTGAGTA GTGTATACGG CGACCAAGCT GCTCTTGCCC GGCGTCTATA CGGGACAACA CCGCGCCACA TAGCAGTACT TTGAAAGTGC TCATCATCGG GAATCGTTCT TCGGGGCGGA AAGACTCAAG GATCTTGCCG CTATTGAGAT CCAGTTCGAT ATAGCCCACT CTTGCACCCA GTTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCGGG GTGTGCAAAA ACAGGCAAGC AAAATGCCGC AAAGAAGGGA ATGAGTGCGA CACGAAAATG TTGGATGCTC ATACTCGTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTACTAGTA CGTCTCTCAA GGATAAGTAA GTAATATTAA GGTACGGGAG GTATTGGACA GGCCGCAATA AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT GTGTGAATCG ATAGTACTAA CATACGCTCT CCATCAAAAC AAAACGAAAC AAAACAAACT AGCAAAATAG GCTGTCCCCA GTGCAAGTGC AGGTGCCAGA ACATTTCTCT
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