NM_181672.3;HINCUT-1; HRNT1; MRX106; O-GLCNAC; OGT1;XLID106
100μg liquid plasmid(Instantly):$120
| name | pCMV-EYFP-OGT(human)-Neo | another name | apCMV-EYFP-OGT(human)-Neo |
| Insert Size (bp) | 3141bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | Yellow |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCT TCGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGGAATTC ATGGCGTCTT CCGTGGGCAA CGTGGCCGAC AGCACAGAAC CAACGAAACG TATGCTTTCC TTCCAAGGGT TAGCTGAGTT GGCACATCGA GAATATCAGG CAGGAGATTT TGAGGCAGCT GAGAGACACT GCATGCAGCT CTGGAGACAA GAGCCAGACA ATACTGGTGT GCTTTTATTA CTTTCATCTA TACACTTCCA GTGTCGAAGG CTGGACAGAT CTGCTCACTT TAGCACTCTG GCAATTAAAC AGAACCCCCT TCTGGCAGAA GCTTATTCGA ATTTGGGGAA TGTGTACAAG GAAAGAGGGC AGTTGCAGGA GGCAATTGAG CATTATCGAC ATGCATTGCG TCTCAAACCT GATTTCATCG ATGGTTATAT TAACCTGGCA GCCGCCTTGG TAGCAGCGGG TGACATGGAA GGGGCAGTAC AAGCTTACGT CTCTGCTCTT CAGTACAATC CTGATTTGTA CTGTGTTCGC AGTGACCTGG GGAACCTGCT CAAAGCCCTG GGTCGCTTGG AAGAAGCCAA GGCATGTTAT TTGAAAGCAA TTGAGACGCA ACCGAACTTT GCAGTAGCTT GGAGTAATCT TGGCTGTGTT TTCAATGCAC AAGGGGAAAT TTGGCTTGCA ATTCATCACT TTGAAAAGGC TGTCACCCTT GACCCAAACT TTCTGGATGC TTATATCAAT TTAGGAAATG TCTTGAAAGA GGCACGCATT TTTGACAGAG CTGTGGCAGC TTATCTTCGT GCCCTAAGTT TGAGTCCAAA TCACGCAGTG GTGCACGGCA ACCTGGCTTG TGTATACTAT GAGCAAGGCC TGATAGATCT GGCAATAGAC ACCTACAGGC GGGCTATCGA ACTACAACCA CATTTCCCTG ATGCTTACTG CAACCTAGCC AATGCTCTCA AAGAGAAGGG CAGTGTTGCT GAAGCAGAAG ATTGTTATAA TACAGCTCTC CGTCTGTGTC CCACCCATGC AGACTCTCTG AATAACCTAG CCAATATCAA ACGAGAACAG GGAAACATTG AAGAGGCAGT TCGCTTGTAT CGTAAAGCAT TAGAAGTCTT CCCAGAGTTT GCTGCTGCCC ATTCAAATTT AGCAAGTGTA CTGCAGCAGC AGGGAAAACT GCAGGAAGCT CTGATGCATT ATAAGGAGGC TATTCGAATC AGTCCTACCT TTGCTGATGC CTACTCTAAT ATGGGAAACA CTCTAAAGGA GATGCAGGAT GTTCAGGGAG CCTTGCAGTG TTATACGCGT GCCATCCAAA TTAATCCTGC ATTTGCAGAT GCACATAGCA ATCTGGCTTC CATTCATAAG GATTCAGGGA ATATTCCAGA AGCCATAGCT TCTTACCGCA CGGCTCTGAA ACTTAAGCCT GATTTTCCTG ATGCTTATTG TAACTTGGCT CATTGCCTGC AGATTGTCTG TGATTGGACA GACTATGATG AGCGAATGAA GAAGTTGGTC AGTATTGTGG CTGACCAGTT AGAGAAGAAT AGGTTGCCTT CTGTGCATCC TCATCATAGT ATGCTATATC CTCTTTCTCA TGGCTTCAGG AAGGCTATTG CTGAGAGGCA CGGCAACCTG TGCTTAGATA AGATTAATGT TCTTCATAAA CCACCATATG AACATCCAAA AGACTTGAAG CTCAGTGATG GTCGGCTGCG TGTAGGATAT GTGAGTTCCG ACTTTGGGAA TCATCCTACT TCTCACCTTA TGCAGTCTAT TCCAGGCATG CACAATCCTG ATAAATTTGA GGTGTTCTGT TATGCCCTGA GCCCAGACGA TGGCACAAAC TTCCGAGTGA AGGTGATGGC AGAAGCCAAT CATTTCATTG ATCTTTCTCA GATTCCATGC AATGGAAAAG CAGCTGATCG CATCCATCAG GATGGAATTC ATATCCTTGT AAATATGAAT GGCTATACTA AGGGCGCTCG AAATGAGCTT TTTGCTCTCA GGCCAGCTCC TATTCAGGCA ATGTGGCTGG GATACCCTGG GACGAGTGGT GCGCTTTTCA TGGATTATAT TATCACTGAT CAGGAAACTT CGCCAGCTGA AGTTGCTGAG CAGTATTCCG AGAAATTGGC TTATATGCCC CACACTTTTT TTATTGGTGA TCATGCTAAT ATGTTCCCTC ACCTGAAGAA AAAAGCAGTC ATCGATTTTA AGTCCAATGG GCACATTTAT GACAATCGGA TAGTTCTGAA TGGCATCGAC CTCAAAGCAT TTCTTGATAG TCTACCAGAT GTGAAAATTG TCAAGATGAA GTGTCCTGAT GGAGGAGACA ATGCAGATAG CAGTAACACA GCTCTTAATA TGCCTGTTAT TCCTATGAAT ACTATTGCAG AAGCAGTTAT TGAAATGATT AACCGAGGAC AGATTCAAAT AACAATTAAT GGATTCAGTA TTAGCAATGG ACTGGCAACT ACTCAGATCA ACAATAAGGC TGCAACTGGA GAGGAGGTTC CCCGTACCAT TATTGTAACC ACCCGTTCTC AGTACGGGTT ACCAGAAGAT GCCATCGTAT ACTGTAACTT TAATCAGTTG TATAAAATTG ACCCTTCTAC TTTGCAGATG TGGGCAAACA TTCTGAAGCG TGTTCCCAAT AGTGTACTCT GGCTGTTGCG TTTTCCAGCA GTAGGAGAAC CTAATATTCA ACAGTATGCA CAAAACATGG GCCTGCCCCA GAACCGTATC ATTTTTTCAC CTGTTGCTCC TAAAGAGGAA CACGTCAGGA GAGGCCAGCT GGCTGATGTC TGCTTGGACA CTCCACTCTG TAATGGGCAC ACCACAGGGA TGGATGTCCT CTGGGCAGGG ACCCCCATGG TGACTATGCC AGGAGAGACT CTTGCTTCTC GAGTTGCAGC ATCCCAGCTC ACTTGCTTAG GTTGTCTTGA GCTTATTGCT AAAAACAGAC AAGAATATGA AGACATAGCT GTGAAGCTGG GAACTGATCT AGAATACCTG AAGAAAGTTC GTGGCAAAGT CTGGAAGCAA AGAATATCTA GCCCTCTGTT CAACACCAAA CAATACACAA TGGAACTAGA GCGGCTCTAT CTACAGATGT GGGAGCATTA TGCAGCTGGC AACAAACCTG ACCACATGAT TAAGCCTGTT GAAGTCACTG AGTCAGCAAC CGGTTAGCTC GAGTCTAGAG GGCCCGTTTA AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA TGGT
