pPH-FLAG-METTL14(human)-P10-6×His-METTL3(human)-Gen
$99.00
In stock
SKU
PL50342
100μg liquid plasmid(Instantly):$120
| name | pPH-FLAG-METTL14(human)-P10-6×His-METTL3(human)-Gen | another name | apPH-FLAG-METTL14(human)-P10-6×His-METTL3(human)-Gen |
| Insert Size (bp) | 3108bp | Plasmid host | Insect cells |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp,Gen |
| Selective marker | Fluorescent labeling | ||
| Promoter | PH | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AAAGTAGCCG AAGATGACGG TTTGTCACAT GGAGTTGGCA GGATGTTTGA TTAAAAACAT AACAGGAAGA AAAATGCCCC GCTGTGGGCG GACAAAATAG TTGGGAACTG GGAGGGGTGG AAATGGAGTT TTTAAGGATT ATTTAGGGAA GAGTGACAAA ATAGATGGGA ACTGGGTGTA GCGTCGTAAG CTAATACGAA AATTAAAAAT GACAAAATAG TTTGGAACTA GATTTCACTT ATCTGGTTCG GATCTCCTAG GCTCAAGCAG TGATCAGATC CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCCTC TAGTACTTCT CGACAAGCTT GTCGAGACTG CAGGCTCTAG ATTCGAAAGC GGCCGCGACT AGTTAGTGAT GATGATGATG ATGTCGAGGT GGAAAGCCAC CTCTGTGTGC TCCTCCACGG CCCCCTCTAA AGCCACCTCT TTCTCCTCGG AAGTTAGATC TATTTCTTTC TCGTCCACGG CCAGCAGAAG TTCCACCTCT TCCTCCACCT CTGGGAGCTC CACCTCCTCG GTCAGATTTA GATTTGGGAG GAGGCGATTT TGGTCGAAGT CTCTCAATTT CTTCTGTACA ACCAGTCAAG TAGGAATTAG GAGCACTGAA ATAGGATGCA TATGTTTCTG CATTGTAGTT GCTATTTGTA AGCGTTGGTC CAACTGTGAG CCAGCCTGGT CGAATTGTAC TATCTCTTCC AAATAGATGA AGGCGTCTTC TACCAAGACA AAAATGCTCA ATTATATGAA AAATTTCTAC AGGTTTTTCT ATATTGCCAA TTTCAGGTTC TTCTGTGATA ATTAAGTCAA TGTCAACATT AGCATGAATG AAGTCCCCGT CTGTGCTACG CTTCACAGTT CCTTTGATCC CCATGAGGCA GTGTTCCTTT GTTCTCTGAA AGACAGCCTT TGGATCTAAA GTCTTAGTCT TCCCAGGATT GTTTTTATTG GTTTTAATCC AACAAATATC TTCACATCTT CTGTAACCCC ATTTTCGTAA ACACACTCTT CCAAGGTCCA ACCCCTCCCC AGAACCACAC CAGAGAAAAA TAAATGATCG AGGTGCTGCA ATCTCATCAA TTTCTAACTT CATAATATCA TCCCAAGTCC AGCATTTTTC ATTAGCAGTG ATGCCAGTTT CTCTGTAATA TTCTTCTAAA GGGGGTTCCA GAAGAATCAC ATCAAATTTG GGTGTTAGTT CTCTGATGTC AAAGGCTTCT ATATCGGCTT GTAAGTACAT GGGAGGAGTG TTAGATTTAG CTATTAACTC ATCCTTTAGC CTGATGAGCT CCCTCAGTTT AGGATATTCT TCAAATCTGT CAGCTAAACC TACATCCCTG ATGAAATTCT GAGGTCTATG TCCAGTGTCT ACAAAATGTT GGCAGTAATC ATTATGGGGA TTTAAGCTCT GTGTTCCCTT AAGAAAAGTA CTAGAATCTT TGTAAATCTC TTCTTCATAT GGCAAATTTT CTTCATCCTG TTGCATTTCT AGTTCATCCT TATATTCTTC CATTTTGTCT TCATCTGTCT CTCCTTCATC CAGATACTTA CGTTTTGCAT TTGGAGCAGA GGTATCATAG GAAGCCCTGC AAGTTTCTCT TGTTTCAGCA ATTTCTCTCT GCTCATCTTT GCTATTTAAC ACGGCACCAA TGCTGTCGGC ACTTTCAGCT CCCAACTGCT GCGCGAGGAG CTGTCGCCGT AACTTCTGCC GCTCCCGGAT CTCCTGCAAG CGGCTATCCA TACCTTGAAA ATACAGGTTT TCCTTATCAT CGTCGTCCTT GTAGTCCATG TCGACGTAGG CCTTTGAATT CCGCGCGCTT CGGACCGGGA TCCGCGCCCG ATGGTGGGAC GGTATGAATA ATCCGGAATA TTTATAGGTT TTTTTATTAC AAAACTGTTA CGAAAACAGT AAAATACTTA TTTATTTGCG AGATGGTTAT CATTTTAATT ATCTCCATGA TCTATTAATA TTCCGGAGTA TACGGACCTT TAATTCAACC CAACACAATA TATTATAGTT AAATAAGAAT TATTATCAAA TCATTTGTAT ATTAATTAAA ATACTATACT GTAAATTACA TTTTATTTAC AATCACTCGA CGAAGACTTG ATCACCCGGG ATCTCGAGAT GCATCATCAC CATCACCATG AGGATCTGTA CTTTCAGAGC ATGTCGGACA CGTGGAGCTC TATCCAGGCC CACAAGAAGC AGCTGGACTC TCTGCGGGAG AGGCTGCAGC GGAGGCGGAA GCAGGACTCG GGGCACTTGG ATCTACGGAA TCCAGAGGCA GCATTGTCTC CAACCTTCCG TAGTGACAGC CCAGTGCCTA CTGCACCCAC CTCTGGTGGC CCTAAGCCCA GCACAGCTTC AGCAGTTCCT GAATTAGCTA CAGATCCTGA GTTAGAGAAG AAGTTGCTAC ACCACCTCTC TGATCTGGCC TTAACATTGC CCACTGATGC TGTGTCCATC TGTCTTGCCA TCTCCACGCC AGATGCTCCT GCCACTCAAG ATGGGGTAGA AAGCCTCCTG CAGAAGTTTG CAGCTCAGGA GTTGATTGAG GTAAAGCGAG GTCTCCTACA AGATGATGCA CATCCTACTC TTGTAACCTA TGCTGACCAT TCCAAGCTCT CTGCCATGAT GGGTGCTGTG GCAGAAAAGA AGGGCCCTGG GGAGGTAGCA GGGACTGTCA CAGGGCAGAA GCGGCGTGCA GAACAGGACT CGACTACAGT AGCTGCCTTT GCCAGTTCGT TAGTCTCTGG TCTGAACTCT TCAGCATCGG AACCAGCAAA GGAGCCAGCC AAGAAATCAA GGAAACATGC TGCCTCAGAT GTTGATCTGG AGATAGAGAG CCTTCTGAAC CAACAGTCCA CTAAGGAACA ACAGAGCAAG AAGGTCAGTC AGGAGATCCT AGAGCTATTA AATACTACAA CAGCCAAGGA ACAATCCATT GTTGAAAAAT TTCGCTCTCG AGGTCGGGCC CAAGTGCAAG AATTCTGTGA CTATGGAACC AAGGAGGAGT GCATGAAAGC CAGTGATGCT GATCGACCCT GTCGCAAGCT GCACTTCAGA CGAATTATCA ATAAACACAC TGATGAGTCT TTAGGTGACT GCTCTTTCCT TAATACATGT TTCCACATGG ATACCTGCAA GTATGTTCAC TATGAAATTG ATGCTTGCAT GGATTCTGAG GCCCCTGGCA GCAAAGACCA CACGCCAAGC CAGGAGCTTG CTCTTACACA GAGTGTCGGA GGTGATTCCA GTGCAGACCG ACTCTTCCCA CCTCAGTGGA TCTGTTGTGA TATCCGCTAC CTGGACGTCA GTATCTTGGG CAAGTTTGCA GTTGTGATGG CTGACCCACC CTGGGATATT CACATGGAAC TGCCCTATGG GACCCTGACA GATGATGAGA TGCGCAGGCT CAACATACCC GTACTACAGG ATGATGGCTT TCTCTTCCTC TGGGTCACAG GCAGGGCCAT GGAGTTGGGG AGAGAATGTC TAAACCTCTG GGGGTATGAA CGGGTAGATG AAATTATTTG GGTGAAGACA AATCAACTGC AACGCATCAT TCGGACAGGC CGTACAGGTC ACTGGTTGAA CCATGGGAAG GAACACTGCT TGGTTGGTGT CAAAGGAAAT CCCCAAGGCT TCAACCAGGG TCTGGATTGT GATGTGATCG TAGCTGAGGT TCGTTCCACC AGTCATAAAC CAGATGAAAT CTATGGCATG ATTGAAAGAC TATCTCCTGG CACTCGCAAG ATTGAGTTAT TTGGACGACC ACACAATGTG CAACCCAACT GGATCACCCT TGGAAACCAA CTGGATGGGA TCCACCTACT AGACCCAGAT GTGGTTGCAC GGTTCAAGCA AAGGTACCCA GATGGTATCA TCTCTAAACC TAAGAATTTA TAGCTAGCAG CTGATGCATA GCATGCGGTA CCGGGAGATG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGAC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCAACCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAAGC CCTGCCATAG CCACTACGGG TACGTAGGCC AACCACTAGA ACTATAGCTA GAGTCCTGGG CGAACAAACG ATGCTCGCCT TCCAGAAAAC CGAGGATGCG AACCACTTCA TCCGGGGTCA GCACCACCGG CAAGCGCCGC GACGGCCGAG GTCTACCGAT CTCCTGAAGC CAGGGCAGAT CCGTGCACAG CACCTTGCCG TAGAAGAACA GCAAGGCCGC CAATGCCTGA CGATGCGTGG AGACCGAAAC CTTGCGCTCG TTCGCCAGCC AGGACAGAAA TGCCTCGACT TCGCTGCTGC CCAAGGTTGC CGGGTGACGC ACACCGTGGA AACGGATGAA GGCACGAACC CAGTTGACAT AAGCCTGTTC GGTTCGTAAA CTGTAATGCA AGTAGCGTAT GCGCTCACGC AACTGGTCCA GAACCTTGAC CGAACGCAGC GGTGGTAACG GCGCAGTGGC GGTTTTCATG GCTTGTTATG ACTGTTTTTT TGTACAGTCT ATGCCTCGGG CATCCAAGCA GCAAGCGCGT TACGCCGTGG GTCGATGTTT GATGTTATGG AGCAGCAACG ATGTTACGCA GCAGCAACGA TGTTACGCAG CAGGGCAGTC GCCCTAAAAC AAAGTTAGGT GGCTCAAGTA TGGGCATCAT TCGCACATGT AGGCTCGGCC CTGACCAAGT CAAATCCATG CGGGCTGCTC TTGATCTTTT CGGTCGTGAG TTCGGAGACG TAGCCACCTA CTCCCAACAT CAGCCGGACT CCGATTACCT CGGGAACTTG CTCCGTAGTA AGACATTCAT CGCGCTTGCT GCCTTCGACC AAGAAGCGGT TGTTGGCGCT CTCGCGGCTT ACGTTCTGCC CAGGTTTGAG CAGCCGCGTA GTGAGATCTA TATCTATGAT CTCGCAGTCT CCGGCGAGCA CCGGAGGCAG GGCATTGCCA CCGCGCTCAT CAATCTCCTC AAGCATGAGG CCAACGCGCT TGGTGCTTAT GTGATCTACG TGCAAGCAGA TTACGGTGAC GATCCCGCAG TGGCTCTCTA TACAAAGTTG GGCATACGGG AAGAAGTGAT GCACTTTGAT ATCGACCCAA GTACCGCCAC CTAACAATTC GTTCAAGCCG AGATCGGCTT CCCGGCCGCG GAGTTGTTCG GTAAATTGTC ACAACGCCGC GAATATAGTC TTTACCATGC CCTTGGCCAC GCCCCTCTTT AATACGACGG GCAATTTGCA CTTCAGAAAA TGAAGAGTTT GCTTTAGCCA TAACAAAAGT CCAGTATGCT TTTTCACAGC ATAACTGGAC TGATTTCAGT TTACAACTAT TCTGTCTAGT TTAAGACTTT ATTGTCATAG TTTAGATCTA TTTTGTTCAG TTTAAGACTT TATTGTCCGC CCACACCCGC TTACGCAGGG CATCCATTTA TTACTCAACC GTAACCGATT TTGCCAGGTT ACGCGGCTGG TCTGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAA ATTGTAAACG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTCCCATT CGCCATTCAG GCTGCAAATA AGCGTTGATA TTCAGTCAAT TACAAACATT AATAACGAAG AGATGACAGA AAAATTTTCA TTCTGTGACA GAGAA
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