pLV3-U6-MCS-sgRNA-EF1a-RfxCas13d-Puro
$70.00
In stock
SKU
PL50185
100μg liquid plasmid(Instantly):$91
| name | pLV3-U6-MCS-sgRNA-EF1a-RfxCas13d-Puro | another name | apLV3-U6-MCS-sgRNA-EF1a-RfxCas13d-Puro |
| Insert Size (bp) | Plasmid host | Mammary cell,Lentiviral | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | U6 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | U6-F：GAGGGCCTATTTCCCATGA | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTTGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA GTAGCACCCA CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG ACGCTGACGG TACAGGCCAG ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG CTGAGGGCTA TTGAGGCGCA ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCAGA CCCACCTCCC AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA GAAGGTGGAG AGAGAGACAG AGACAGATCC ATTCGATTAG TGAACGGATC GGCACTGCGT GCGCCAATTC TGCAGACAAA TGGCAGTATT CATCCACAAT TTTAAAAGAA AAGGGGGGAT TGGGGGGTAC AGTGCAGGGG AAAGAATAGT AGACATAATA GCAACAGACA TACAAACTAA AGAATTACAA AAACAAATTA CAAAAATTCA AAATTTTCGG GTTTATTACA GGGACAGCAG AGATCCAGTT TGGTTAATTA AGGTACCGAG GGCCTATTTC CCATGATTCC TTCATATTTG CATATACGAT ACAAGGCTGT TAGAGAGATA ATTAGAATTA ATTTGACTGT AAACACAAAG ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGGAG ACGGTTGTAA ATGAGCACAC AAAATACACA TGCTAAAATA TTATATTCTA TGACCTTTAT AAAATCAACC AAAATCTTCT TTTTAATAAC TTTAGTATCA ATAATTAGAA TTTTTATGTT CCTTTTTGCA AACTTTTAAT AAAAATGAGC AAAATAAAAA AACGCTAGTT TTAGTAACTC GCGTTGTTTT CTTCACCTTT AATAATAGCT ACTCCACCAC TTGTTCCTAA GCGGTCAGCT CCTGCTTCAA TCATTTTTTG AGCATCTTCA AATGTTCTAA CTCCACCAGC TGCTTTAACT AAAGCATTGT CTTTAACAAC TGACTTCATT AGTTTAACAT CTTCAAATGT TGCACCTGAT TTTGAAAATC CTGTTGATGT TTTAACAAAT TCTAATCCAG CTTCAACAGC TATTTCACAA GCTTTCATGA TTTCTTCTTT TGTTAATAAA CAATTTTCCA TAATACATTT AACAACATGT GATCCAGCTG CTTTTTTTAC AGCTTTCATG TCTTCTAAAA CTAATTCATA ATTTTTGTCT TTTAATGCAC CAATATTTAA TACCATATCA ATTTCTGTTG CACCATCTTT AATTGCTTCA GAAACTTCGA ATGCTTTTGT AGCTGTTGTG CATGCACCTA GAGGAAAACC TACAACATTT GTTATTCCTA CATTTGTGCC TTTTAATAAT TCTTTACAAT AGCTTGTTCA ATATGAATTA ACACAAACTG TTGCAAAATC AAATTCAATT GCTTCATCAC ATAATTGTTT AATTTCAGCT TTCGTAGCAT CTTGTTTTAA TAATGTGTGA TCTATATATT TGTTTAGTTT CATTTTTTCT CCTATATATT CATTTTTAAT TTTAATTCTT TAATAATTTC GTCTACTTTA ACTTTAGCGT TTTGAACAGA TTCACCAACA CCTATAAAAT AAATTTTTAG TTTAGGTTCA GTTCCACTTG GGCGAACAGC AAATCATGAC TTATCTTCTA AATAAAATTT TAGTAAGTCT TGTCCTGGCA TATTATACAT TCCATCGATG TAGTCTTCAA CATTAACAAC TTTAAGTCCA GCAATTTGAG TTAAGGGTGT TGCTCTCAAT GATTTCATTA ATGGTTCAAT TTTTAATTTC TTTTCTTCTG GTTTAAAATT CAAGTTTAAA GTGAAAGTGT AATATGCACC CATTTCTTTA AATAAATCTT CTAAATAGTC TACTAATGTT TTATTTTGTT TTTTATAAAA TCAAGCAGCC TCTGCTATTA ATATAGAAGC TTGTATTCCA TCTTTATCTC TAGCTGAGTC ATCAATTACA TATCCATAAC TTTCTTCATA AGCAAAAACA AAATTTAATC CGTTATCTTC TTCTTTAGCA ATTTCTCTAC CCATTCATTT AAATCCAGTT AAAGTTTTTA CAATATTAAC TCCATATTTT TCATGAGCGA TTCTATCACC CAAATCACTT GTTACAAAAC TTGAATATAG AGCCGGATTT TTTGGAATGC TATTTAAGCG TTTTAGATTT GATAATTTTC AATCAATTAA AATTGGTCCT GTTTGATTTC CATCTAATCT TACAAAATGA CCATCATGTT TTATTGCCAT TCCAAATCTG TCAGCATCTG GGTCATTCAT AATAATAATA TCTGCATCAT GTTTAATACC ATATTCAAGC GGTATTTTTC ATGCAGGATC AAATTCTGGA TTTGGATTTA CAACATTTTT AAATGTTTCA TCTTCAAATG CATGCTCTTC AACCTCAATA ACGTTATATC CTGATTCACG TAATATTTTT GGGGTAAATT TAGTTCCTGT TCCATTAACT GCGCTAAAAA TAATTTTTAA ATCTTTTTTA GCTTCTTGCT CTTTTTTGTA CGTCTCTGTT TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC ACCGAGTCGG TGCTTTTTTG AATTCGCTAG CTAGGTCTTG AAAGGAGTGG GAATTGGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT TGATCCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGGACC GGTTCTAGAG CGCTGCCACC ATGATCGAAA AAAAAAAGTC CTTCGCCAAG GGCATGGGCG TGAAGTCCAC ACTCGTGTCC GGCTCCAAAG TGTACATGAC AACCTTCGCC GAAGGCAGCG ACGCCAGGCT GGAAAAGATC GTGGAGGGCG ACAGCATCAG GAGCGTGAAT GAGGGCGAGG CCTTCAGCGC TGAAATGGCC GATAAAAACG CCGGCTATAA GATCGGCAAC GCCAAATTCA GCCATCCTAA GGGCTACGCC GTGGTGGCTA ACAACCCTCT GTATACAGGA CCCGTCCAGC AGGATATGCT CGGCCTGAAG GAAACTCTGG AAAAGAGGTA CTTCGGCGAG AGCGCTGATG GCAATGACAA TATTTGTATC CAGGTGATCC ATAACATCCT GGACATTGAA AAAATCCTCG CCGAATACAT TACCAACGCC GCCTACGCCG TCAACAATAT CTCCGGCCTG GATAAGGACA TTATTGGATT CGGCAAGTTC TCCACAGTGT ATACCTACGA CGAATTCAAA GACCCCGAGC ACCATAGGGC CGCTTTCAAC AATAACGATA AGCTCATCAA CGCCATCAAG GCCCAGTATG ACGAGTTCGA CAACTTCCTC GATAACCCCA GACTCGGCTA TTTCGGCCAG GCCTTTTTCA GCAAGGAGGG CAGAAATTAC ATCATCAATT ACGGCAACGA ATGCTATGAC ATTCTGGCCC TCCTGAGCGG ACTGAGGCAC TGGGTGGTCC ATAACAACGA AGAAGAGTCC AGGATCTCCA GGACCTGGCT CTACAACCTC GATAAGAACC TCGACAACGA ATACATCTCC ACCCTCAACT ACCTCTACGA CAGGATCACC AATGAGCTGA CCAACTCCTT CTCCAAGAAC TCCGCCGCCA ACGTGAACTA TATTGCCGAA ACTCTGGGAA TCAACCCTGC CGAATTCGCC GAACAATATT TCAGATTCAG CATTATGAAA GAGCAGAAAA ACCTCGGATT CAATATCACC AAGCTCAGGG AAGTGATGCT GGACAGGAAG GATATGTCCG AGATCAGGAA AAATCATAAG GTGTTCGACT CCATCAGGAC CAAGGTCTAC ACCATGATGG ACTTTGTGAT TTATAGGTAT TACATCGAAG AGGATGCCAA GGTGGCTGCC GCCAATAAGT CCCTCCCCGA TAATGAGAAG TCCCTGAGCG AGAAGGATAT CTTTGTGATT AACCTGAGGG GCTCCTTCAA CGACGACCAG AAGGATGCCC TCTACTACGA TGAAGCTAAT AGAATTTGGA GAAAGCTCGA AAATATCATG CACAACATCA AGGAATTTAG GGGAAACAAG ACAAGAGAGT ATAAGAAGAA GGACGCCCCT AGACTGCCCA GAATCCTGCC CGCTGGCCGT GATGTTTCCG CCTTCAGCAA ACTCATGTAT GCCCTGACCA TGTTCCTGGA TGGCAAGGAG ATCAACGACC TCCTGACCAC CCTGATTAAT AAATTCGATA ACATCCAGAG CTTCCTGAAG GTGATGCCTC TCATCGGAGT CAACGCTAAG TTCGTGGAGG AATACGCCTT TTTCAAAGAC TCCGCCAAGA TCGCCGATGA GCTGAGGCTG ATCAAGTCCT TCGCTAGAAT GGGAGAACCT ATTGCCGATG CCAGGAGGGC CATGTATATC GACGCCATCC GTATTTTAGG AACCAACCTG TCCTATGATG AGCTCAAGGC CCTCGCCGAC ACCTTTTCCC TGGACGAGAA CGGAAACAAG CTCAAGAAAG GCAAGCACGG CATGAGAAAT TTCATTATTA ATAACGTGAT CAGCAATAAA AGGTTCCACT ACCTGATCAG ATACGGTGAT CCTGCCCACC TCCATGAGAT CGCCAAAAAC GAGGCCGTGG TGAAGTTCGT GCTCGGCAGG ATCGCTGACA TCCAGAAAAA ACAGGGCCAG AACGGCAAGA ACCAGATCGA CAGGTACTAC GAAACTTGTA TCGGAAAGGA TAAGGGCAAG AGCGTGAGCG AAAAGGTGGA CGCTCTCACA AAGATCATCA CCGGAATGAA CTACGACCAA TTCGACAAGA AAAGGAGCGT CATTGAGGAC ACCGGCAGGG AAAACGCCGA GAGGGAGAAG TTTAAAAAGA TCATCAGCCT GTACCTCACC GTGATCTACC ACATCCTCAA GAATATTGTC AATATCAACG CCAGGTACGT CATCGGATTC CATTGCGTCG AGCGTGATGC TCAACTGTAC AAGGAGAAAG GCTACGACAT CAATCTCAAG AAACTGGAAG AGAAGGGATT CAGCTCCGTC ACCAAGCTCT GCGCTGGCAT TGATGAAACT GCCCCCGATA AGAGAAAGGA CGTGGAAAAG GAGATGGCTG AAAGAGCCAA GGAGAGCATT GACAGCCTCG AGAGCGCCAA CCCCAAGCTG TATGCCAATT ACATCAAATA CAGCGACGAG AAGAAAGCCG AGGAGTTCAC CAGGCAGATT AACAGGGAGA AGGCCAAAAC CGCCCTGAAC GCCTACCTGA GGAACACCAA GTGGAATGTG ATCATCAGGG AGGACCTCCT GAGAATTGAC AACAAGACAT GTACCCTGTT CAGAAACAAG GCCGTCCACC TGGAAGTGGC CAGGTATGTC CACGCCTATA TCAACGACAT TGCCGAGGTC AATTCCTACT TCCAACTGTA CCATTACATC ATGCAGAGAA TTATCATGAA TGAGAGGTAC GAGAAAAGCA GCGGAAAGGT GTCCGAGTAC TTCGACGCTG TGAATGACGA GAAGAAGTAC AACGATAGGC TCCTGAAACT GCTGTGTGTG CCTTTCGGCT ACTGTATCCC CAGGTTTAAG AACCTGAGCA TCGAGGCCCT GTTCGATAGG AACGAGGCCG CCAAGTTCGA CAAGGAGAAA AAGAAGGTGT CCGGCAATCT TCAACTGCCT CCACTTGAAA GACTGACACT GGGTAGTTCC GGATCCGGCG CAACAAACTT CTCTCTGCTG AAACAAGCCG GAGATGTCGA AGAGAATCCT GGACCGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGA GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAACGCGTT AAGTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCGTC GACTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATCTGCTTT TTGCTTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGAC
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