pLV2-U6-KOMP(mouse)-sgRNA1-Cas9-Neo
$70.00
In stock
SKU
PL50071
100μg liquid plasmid(Instantly):$91
| name | pLV2-U6-KOMP(mouse)-sgRNA1-Cas9-Neo | another name | apLV2-U6-KOMP(mouse)-sgRNA1-Cas9-Neo |
| Insert Size (bp) | Plasmid host | Mammary cell,Lentiviral | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | U6 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGCGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GCGCGTTTTG CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA GGGAAACCAG AGGAGCTCTC TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA GGCGAGGGGC GGCGACTGGT GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG AGAGATGGGT GCGAGAGCGT CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA ATTCGGTTAA GGCCAGGGGG AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC AGGGAGCTAG AACGATTCGC AGTTAATCCT GGCCTGTTAG AAACATCAGA AGGCTGTAGA CAAATACTGG GACAGCTACA ACCATCCCTT CAGACAGGAT CAGAAGAACT TAGATCATTA TATAATACAG TAGCAACCCT CTATTGTGTG CATCAAAGGA TAGAGATAAA AGACACCAAG GAAGCTTTAG ACAAGATAGA GGAAGAGCAA AACAAAAGTA AGACCACCGC ACAGCAAGCG GCCGCTGATC TTCAGACCTG GAGGAGGAGA TATGAGGGAC AATTGGAGAA GTGAATTATA TAAATATAAA GTAGTAAAAA TTGAACCATT AGGAGTAGCA CCCACCAAGG CAAAGAGAAG AGTGGTGCAG AGAGAAAAAA GAGCAGTGGG AATAGGAGCT TTGTTCCTTG GGTTCTTGGG AGCAGCAGGA AGCACTATGG GCGCAGCGTC AATGACGCTG ACGGTACAGG CCAGACAATT ATTGTCTGGT ATAGTGCAGC AGCAGAACAA TTTGCTGAGG GCTATTGAGG CGCAACAGCA TCTGTTGCAA CTCACAGTCT GGGGCATCAA GCAGCTCCAG GCAAGAATCC TGGCTGTGGA AAGATACCTA AAGGATCAAC AGCTCCTGGG GATTTGGGGT TGCTCTGGAA AACTCATTTG CACCACTGCT GTGCCTTGGA ATGCTAGTTG GAGTAATAAA TCTCTGGAAC AGATTTGGAA TCACACGACC TGGATGGAGT GGGACAGAGA AATTAACAAT TACACAAGCT TAATACACTC CTTAATTGAA GAATCGCAAA ACCAGCAAGA AAAGAATGAA CAAGAATTAT TGGAATTAGA TAAATGGGCA AGTTTGTGGA ATTGGTTTAA CATAACAAAT TGGCTGTGGT ATATAAAATT ATTCATAATG ATAGTAGGAG GCTTGGTAGG TTTAAGAATA GTTTTTGCTG TACTTTCTAT AGTGAATAGA GTTAGGCAGG GATATTCACC ATTATCGTTT CAGACCCACC TCCCAACCCC GAGGGGACCC GACAGGCCCG AAGGAATAGA AGAAGAAGGT GGAGAGAGAG ACAGAGACAG ATCCATTCGA TTAGTGAACG GATCGGCACT GCGTGCGCCA ATTCTGCAGA CAAATGGCAG TATTCATCCA CAATTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TCGGGTTTAT TACAGGGACA GCAGAGATCC AGTTTGGTTA ATTAAGGTAC CGAGGGCCTA TTTCCCATGA TTCCTTCATA TTTGCATATA CGATACAAGG CTGTTAGAGA GATAATTAGA ATTAATTTGA CTGTAAACAC AAAGATATTA GTACAAAATA CGTGACGTAG AAAGTAATAA TTTCTTGGGT AGTTTGCAGT TTTAAAATTA TGTTTTAAAA TGGACTATCA TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAACACC GGGATGGAGA GTAACTAGAC AAGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG TCCGTTATCA ACTTGAAAAA GTGGCACCGA GTCGGTGCTT TTTTGAATTC GCTAGCTAGG TCTTGAAAGG AGTGGGAATT GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGATC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGACCGGTTC TAGAGCGCTG CCACCATGGA CAAGAAGTAC AGCATCGGCC TGGACATCGG CACCAACTCT GTGGGCTGGG CCGTGATCAC CGACGAGTAC AAGGTGCCCA GCAAGAAATT CAAGGTGCTG GGCAACACCG ACCGGCACAG CATCAAGAAG AACCTGATCG GAGCCCTGCT GTTCGACAGC GGCGAAACAG CCGAGGCCAC CCGGCTGAAG AGAACCGCCA GAAGAAGATA CACCAGACGG AAGAACCGGA TCTGCTATCT GCAAGAGATC TTCAGCAACG AGATGGCCAA GGTGGACGAC AGCTTCTTCC ACAGACTGGA AGAGTCCTTC CTGGTGGAAG AGGATAAGAA GCACGAGCGG CACCCCATCT TCGGCAACAT CGTGGACGAG GTGGCCTACC ACGAGAAGTA CCCCACCATC TACCACCTGA GAAAGAAACT GGTGGACAGC ACCGACAAGG CCGACCTGCG GCTGATCTAT CTGGCCCTGG CCCACATGAT CAAGTTCCGG GGCCACTTCC TGATCGAGGG CGACCTGAAC CCCGACAACA GCGACGTGGA CAAGCTGTTC ATCCAGCTGG TGCAGACCTA CAACCAGCTG TTCGAGGAAA ACCCCATCAA CGCCAGCGGC GTGGACGCCA AGGCCATCCT GTCTGCCAGA CTGAGCAAGA GCAGACGGCT GGAAAATCTG ATCGCCCAGC TGCCCGGCGA GAAGAAGAAT GGCCTGTTCG GAAACCTGAT TGCCCTGAGC CTGGGCCTGA CCCCCAACTT CAAGAGCAAC TTCGACCTGG CCGAGGATGC CAAACTGCAG CTGAGCAAGG ACACCTACGA CGACGACCTG GACAACCTGC TGGCCCAGAT CGGCGACCAG TACGCCGACC TGTTTCTGGC CGCCAAGAAC CTGTCCGACG CCATCCTGCT GAGCGACATC CTGAGAGTGA ACACCGAGAT CACCAAGGCC CCCCTGAGCG CCTCTATGAT CAAGAGATAC GACGAGCACC ACCAGGACCT GACCCTGCTG AAAGCTCTCG TGCGGCAGCA GCTGCCTGAG AAGTACAAAG AGATTTTCTT CGACCAGAGC AAGAACGGCT ACGCCGGCTA CATTGACGGC GGAGCCAGCC AGGAAGAGTT CTACAAGTTC ATCAAGCCCA TCCTGGAAAA GATGGACGGC ACCGAGGAAC TGCTCGTGAA GCTGAACAGA GAGGACCTGC TGCGGAAGCA GCGGACCTTC GACAACGGCA GCATCCCCCA CCAGATCCAC CTGGGAGAGC TGCACGCCAT TCTGCGGCGG CAGGAAGATT TTTACCCATT CCTGAAGGAC AACCGGGAAA AGATCGAGAA GATCCTGACC TTCCGCATCC CCTACTACGT GGGCCCTCTG GCCAGGGGAA ACAGCAGATT CGCCTGGATG ACCAGAAAGA GCGAGGAAAC CATCACCCCC TGGAACTTCG AGGAAGTGGT GGACAAGGGC GCTTCCGCCC AGAGCTTCAT CGAGCGGATG ACCAACTTCG ATAAGAACCT GCCCAACGAG AAGGTGCTGC CCAAGCACAG CCTGCTGTAC GAGTACTTCA CCGTGTATAA CGAGCTGACC AAAGTGAAAT ACGTGACCGA GGGAATGAGA AAGCCCGCCT TCCTGAGCGG CGAGCAGAAA AAGGCCATCG TGGACCTGCT GTTCAAGACC AACCGGAAAG TGACCGTGAA GCAGCTGAAA GAGGACTACT TCAAGAAAAT CGAGTGCTTC GACTCCGTGG AAATCTCCGG CGTGGAAGAT CGGTTCAACG CCTCCCTGGG CACATACCAC GATCTGCTGA AAATTATCAA GGACAAGGAC TTCCTGGACA ATGAGGAAAA CGAGGACATT CTGGAAGATA TCGTGCTGAC CCTGACACTG TTTGAGGACA GAGAGATGAT CGAGGAACGG CTGAAAACCT ATGCCCACCT GTTCGACGAC AAAGTGATGA AGCAGCTGAA GCGGCGGAGA TACACCGGCT GGGGCAGGCT GAGCCGGAAG CTGATCAACG GCATCCGGGA CAAGCAGTCC GGCAAGACAA TCCTGGATTT CCTGAAGTCC GACGGCTTCG CCAACAGAAA CTTCATGCAG CTGATCCACG ACGACAGCCT GACCTTTAAA GAGGACATCC AGAAAGCCCA GGTGTCCGGC CAGGGCGATA GCCTGCACGA GCACATTGCC AATCTGGCCG GCAGCCCCGC CATTAAGAAG GGCATCCTGC AGACAGTGAA GGTGGTGGAC GAGCTCGTGA AAGTGATGGG CCGGCACAAG CCCGAGAACA TCGTGATCGA AATGGCCAGA GAGAACCAGA CCACCCAGAA GGGACAGAAG AACAGCCGCG AGAGAATGAA GCGGATCGAA GAGGGCATCA AAGAGCTGGG CAGCCAGATC CTGAAAGAAC ACCCCGTGGA AAACACCCAG CTGCAGAACG AGAAGCTGTA CCTGTACTAC CTGCAGAATG GGCGGGATAT GTACGTGGAC CAGGAACTGG ACATCAACCG GCTGTCCGAC TACGATGTGG ACCATATCGT GCCTCAGAGC TTTCTGAAGG ACGACTCCAT CGACAACAAG GTGCTGACCA GAAGCGACAA GAACCGGGGC AAGAGCGACA ACGTGCCCTC CGAAGAGGTC GTGAAGAAGA TGAAGAACTA CTGGCGGCAG CTGCTGAACG CCAAGCTGAT TACCCAGAGA AAGTTCGACA ATCTGACCAA GGCCGAGAGA GGCGGCCTGA GCGAACTGGA TAAGGCCGGC TTCATCAAGA GACAGCTGGT GGAAACCCGG CAGATCACAA AGCACGTGGC ACAGATCCTG GACTCCCGGA TGAACACTAA GTACGACGAG AATGACAAGC TGATCCGGGA AGTGAAAGTG ATCACCCTGA AGTCCAAGCT GGTGTCCGAT TTCCGGAAGG ATTTCCAGTT TTACAAAGTG CGCGAGATCA ACAACTACCA CCACGCCCAC GACGCCTACC TGAACGCCGT CGTGGGAACC GCCCTGATCA AAAAGTACCC TAAGCTGGAA AGCGAGTTCG TGTACGGCGA CTACAAGGTG TACGACGTGC GGAAGATGAT CGCCAAGAGC GAGCAGGAAA TCGGCAAGGC TACCGCCAAG TACTTCTTCT ACAGCAACAT CATGAACTTT TTCAAGACCG AGATTACCCT GGCCAACGGC GAGATCCGGA AGCGGCCTCT GATCGAGACA AACGGCGAAA CCGGGGAGAT CGTGTGGGAT AAGGGCCGGG ATTTTGCCAC CGTGCGGAAA GTGCTGAGCA TGCCCCAAGT GAATATCGTG AAAAAGACCG AGGTGCAGAC AGGCGGCTTC AGCAAAGAGT CTATCCTGCC CAAGAGGAAC AGCGATAAGC TGATCGCCAG AAAGAAGGAC TGGGACCCTA AGAAGTACGG CGGCTTCGAC AGCCCCACCG TGGCCTATTC TGTGCTGGTG GTGGCCAAAG TGGAAAAGGG CAAGTCCAAG AAACTGAAGA GTGTGAAAGA GCTGCTGGGG ATCACCATCA TGGAAAGAAG CAGCTTCGAG AAGAATCCCA TCGACTTTCT GGAAGCCAAG GGCTACAAAG AAGTGAAAAA GGACCTGATC ATCAAGCTGC CTAAGTACTC CCTGTTCGAG CTGGAAAACG GCCGGAAGAG AATGCTGGCC TCTGCCGGCG AACTGCAGAA GGGAAACGAA CTGGCCCTGC CCTCCAAATA TGTGAACTTC CTGTACCTGG CCAGCCACTA TGAGAAGCTG AAGGGCTCCC CCGAGGATAA TGAGCAGAAA CAGCTGTTTG TGGAACAGCA CAAGCACTAC CTGGACGAGA TCATCGAGCA GATCAGCGAG TTCTCCAAGA GAGTGATCCT GGCCGACGCT AATCTGGACA AAGTGCTGTC CGCCTACAAC AAGCACCGGG ATAAGCCCAT CAGAGAGCAG GCCGAGAATA TCATCCACCT GTTTACCCTG ACCAATCTGG GAGCCCCTGC CGCCTTCAAG TACTTTGACA CCACCATCGA CCGGAAGAGG TACACCAGCA CCAAAGAGGT GCTGGACGCC ACCCTGATCC ACCAGAGCAT CACCGGCCTG TACGAGACAC GGATCGACCT GTCTCAGCTG GGAGGCGACA AGCGACCTGC CGCCACAAAG AAGGCTGGAC AGGCTAAGAA GAAGAAAGAT TACAAAGACG ATGACGATAA GGGATCCGGC GCAACAAACT TCTCTCTGCT GAAACAAGCC GGAGATGTCG AAGAGAATCC TGGACCGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAA CGCGTTAAGT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCGTCGACT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GAC
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