NM_001174078.1;b2b508.1Clo; b2b692Clo; BAF190A; Brg1;HP172; SNF2beta; SW1/SNF
100μg liquid plasmid(Instantly):$192
| name | pCMV-SMARCA4(mouse)-FLAG-Neo | another name | apCMV-SMARCA4(mouse)-FLAG-Neo |
| Insert Size (bp) | 4854bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CCGCCACCAT GTCTACTCCA GACCCACCCT TGGGTGGGAC TCCTCGGCCT GGTCCTTCCC CAGGCCCTGG TCCTTCACCT GGTGCAATGC TGGGTCCTAG CCCTGGCCCC TCACCAGGTT CTGCCCACAG CATGATGGGG CCAAGCCCAG GACCTCCTTC AGCAGGACAT CCCATGCCCA CCCAGGGGCC TGGAGGGTAC CCCCAGGACA ACATGCATCA GATGCACAAG CCTATGGAGT CCATGCACGA GAAGGGCATG CCTGATGACC CACGATACAA CCAGATGAAA GGGATGGGCA TGCGGTCAGG GGCCCACACA GGCATGGCAC CTCCACCTAG TCCCATGGAC CAGCATTCTC AAGGTTACCC CTCACCCCTC GGCGGCTCTG AACATGCCTC CAGTCCTGTC CCAGCCAGTG GCCCATCTTC AGGCCCCCAG ATGTCCTCTG GGCCAGGAGG GGCCCCACTA GATGGTTCTG ATCCCCAGGC CTTGGGACAG CAAAACAGAG GCCCAACCCC ATTTAACCAG AACCAGCTGC ATCAACTCAG AGCTCAGATA ATGGCCTACA AGATGTTGGC CAGGGGCCAG CCATTGCCCG ACCACCTGCA GATGGCCGTG CAAGGCAAGC GGCCGATGCC TGGAATGCAG CAACAGATGC CAACACTACC TCCACCCTCA GTGTCCGCCA CAGGACCCGG ACCTGGACCC GGCCCTGGCC CTGGCCCTGG CCCAGGACCA GCCCCTCCAA ATTACAGTAG ACCCCATGGT ATGGGAGGGC CCAACATGCC TCCCCCAGGA CCCTCAGGTG TGCCCCCCGG GATGCCTGGT CAGCCGCCTG GAGGGCCTCC CAAGCCATGG CCTGAAGGAC CCATGGCCAA TGCTGCTGCC CCCACAAGCA CCCCACAGAA GCTGATTCCT CCGCAACCAA CAGGCCGTCC TTCACCTGCA CCTCCTGCTG TCCCGCCTGC TGCCTCACCT GTAATGCCAC CACAAACACA GTCCCCAGGG CAGCCAGCCC AGCCTGCTCC ATTGGTGCCA CTGCACCAGA AGCAGAGCCG AATCACCCCC ATCCAGAAGC CCCGAGGCCT TGACCCTGTG GAGATCCTAC AAGAGCGGGA GTACAGGCTT CAGGCTCGAA TCGCACACAG AATTCAGGAA CTTGAAAACC TCCCTGGGTC CCTGGCTGGG GACCTTCGAA CCAAAGCAAC CATCGAACTC AAGGCCCTTA GGTTGCTGAA CTTCCAGAGG CAGCTGCGCC AGGAGGTGGT GGTGTGCATG CGAAGAGACA CAGCCCTGGA GACAGCCCTC AATGCCAAGG CCTACAAGCG CAGCAAACGT CAGTCACTAC GGGAGGCCCG CATCACTGAG AAGTTGGAGA AGCAGCAGAA GATTGAACAG GAGCGCAAGC GCCGCCAGAA GCACCAGGAG TACCTCAACA GCATTCTGCA GCATGCCAAG GACTTCAGGG AGTATCACAG ATCAGTCACA GGCAAACTCC AGAAACTCAC CAAGGCTGTG GCCACCTACC ATGCCAACAC TGAGCGGGAG CAGAAGAAAG AAAATGAGCG CATTGAGAAG GAGCGAATGC GGAGGCTTAT GGCTGAAGAT GAGGAGGGCT ACCGCAAACT CATTGACCAG AAGAAGGACA AGCGCCTGGC CTACCTTCTG CAGCAGACAG ATGAGTATGT GGCCAACCTC ACAGAGCTGG TGCGGCAGCA CAAAGCTGCC CAGGTTGCCA AGGAGAAGAA GAAGAAAAAG AAAAAGAAGA AGGCAGAAAA TGCTGAAGGA CAGACACCTG CTATTGGACC AGATGGTGAG CCTCTGGATG AGACCAGCCA GATGAGTGAC CTCCCTGTGA AGGTGATCCA CGTGGAGAGT GGCAAGATCC TCACTGGCAC AGATGCCCCA AAAGCCGGGC AGCTGGAAGC CTGGCTTGAA ATGAACCCAG GGTATGAAGT AGCCCCCAGG TCAGACAGTG AAGAAAGTGG CTCTGAAGAG GAGGAGGAGG AGGAGGAAGA GGAGCAGCCT CAGCCCGCAC AGCCCCCTAC ACTGCCTGTG GAAGAAAAGA AGAAGATTCC AGACCCAGAC AGCGATGATG TCTCTGAGGT GGACGCCCGA CACATTATTG AGAACGCCAA GCAAGATGTG GACGATGAGT ACGGTGTGTC CCAGGCCCTT GCTCGTGGCC TGCAGTCTTA CTATGCTGTG GCCCATGCAG TCACAGAGAG AGTAGATAAG CAGTCCGCCC TCATGGTCAA CGGTGTCCTC AAACAGTACC AGATCAAGGG TTTGGAGTGG CTGGTGTCCC TGTACAACAA CAACCTGAAT GGCATCCTGG CTGATGAGAT GGGGCTGGGG AAGACCATCC AGACCATCGC GCTCATCACA TACCTCATGG AGCACAAGCG CATCAACGGG CCTTTCCTCA TCATCGTGCC TCTCTCGACA CTGTCAAACT GGGCGTATGA ATTTGACAAG TGGGCCCCCT CTGTGGTGAA GGTTTCTTAC AAGGGCTCTC CAGCTGCAAG GCGAGCTTTT GTCCCACAGC TTCGCAGTGG GAAGTTCAAC GTCTTACTGA CCACCTATGA ATATATCATC AAAGACAAGC ATATCCTAGC CAAGATCCGC TGGAAGTACA TGATTGTGGA TGAAGGCCAC CGCATGAAAA ACCACCACTG CAAGTTGACG CAGGTCCTTA ACACACACTA CGTGGCCCCT CGGCGCCTGC TTCTTACAGG CACACCACTG CAGAACAAGC TACCGGAGCT CTGGGCCCTG CTTAACTTCC TGCTCCCCAC TATCTTCAAG AGCTGCAGCA CCTTCGAACA GTGGTTCAAT GCACCCTTTG CCATGACTGG AGAAAAGGTG GACCTGAATG AAGAGGAGAC TATCCTCATT ATTCGTCGCC TACACAAAGT TCTGCGGCCC TTCCTGCTGC GGCGGCTCAA GAAGGAAGTT GAAGCCCAGC TCCCTGAGAA GGTAGAGTAT GTCATCAAAT GCGACATGTC AGCCCTGCAG CGTGTGCTGT ACCGTCACAT GCAGGCCAAA GGTGTGCTGC TGACTGACGG CTCCGAGAAG GACAAGAAGG GCAAAGGTGG CACCAAGACA CTGATGAACA CTATTATGCA ACTGCGTAAG ATCTGCAACC ACCCCTACAT GTTCCAGCAC ATCGAGGAGT CCTTTTCTGA GCACTTGGGG TTCACCGGCG GCATCGTGCA AGGATTGGAC CTTTACCGTG CCTCAGGGAA ATTTGAACTT CTTGATAGAA TTCTACCCAA ACTCCGTGCA ACGAACCATA AAGTGCTCCT CTTTTGCCAA ATGACCTCCC TCATGACCAT CATGGAAGAC TACTTTGCAT ACCGTGGCTT CAAATACCTC AGGCTTGATG GAACCACAAA AGCAGAAGAC CGGGGCATGC TGTTGAAAAC CTTTAATGAA CCTGGCTCTG AGTATTTCAT TTTCCTGCTC AGTACCCGTG CTGGGGGGCT GGGCCTGAAT CTGCAGTCAG CTGACACTGT GATCATCTTT GACAGTGACT GGAATCCCCA CCAGGACCTG CAAGCACAGG ATCGAGCCCA TCGCATTGGA CAGCAGAATG AGGTGCGTGT TCTTCGCCTG TGCACGGTCA ACAGTGTGGA AGAGAAGATA CTGGCTGCTG CCAAATACAA ACTCAATGTG GATCAGAAGG TGATCCAGGC AGGCATGTTC GACCAGAAGT CGTCCAGCCA TGAGAGGCGT GCCTTCCTGC AGGCCATCCT GGAGCACGAG GAGCAGGATG AGGAGGAAGA TGAGGTGCCT GATGATGAGA CCGTCAACCA GATGATTGCC CGGCACGAAG AAGAGTTTGA CCTCTTCATG CGCATGGACT TGGACCGCCG GCGTGAAGAA GCCCGCAACC CCAAGCGGAA GCCACGCCTG ATGGAAGAGG ATGAGCTCCC ATCCTGGATC ATCAAGGATG ATGCCGAGGT GGAGCGGCTG ACATGTGAAG AGGAAGAGGA GAAGATGTTC GGCCGTGGTT CTCGCCACCG CAAGGAGGTA GACTACAGCG ACTCACTGAC AGAGAAGCAG TGGCTCAAGA CCCTGAAGGC TATCGAGGAG GGCACGCTGG AGGAGATCGA AGAGGAGGTC CGGCAGAAGA AATCTTCACG TAAGCGTAAG CGAGACAGCG AGGCCGGCTC CTCCACCCCG ACCACCAGCA CCCGCAGCCG TGACAAGGAT GAGGAGAGCA AGAAGCAGAA GAAACGTGGG CGGCCACCTG CTGAGAAGCT GTCCCCAAAC CCACCTAACC TCACCAAGAA GATGAAGAAG ATCGTGGATG CTGTGATCAA GTACAAAGAC AGCAGCAGTG GACGTCAGCT CAGCGAGGTG TTCATCCAGC TCCCCTCTCG CAAGGAGCTT CCTGAGTACT ATGAGCTCAT CCGAAAGCCT GTGGACTTCA AGAAGATCAA GGAACGCATC CGAAACCACA AGTACCGCAG CCTCAATGAC CTGGAGAAGG ATGTGATGCT GCTGTGCCAG AACGCTCAGA CGTTCAACCT CGAGGGTTCC CTGATCTATG AGGACTCCAT CGTCCTGCAG TCTGTCTTCA CCAGCGTACG GCAGAAGATT GAGAAGGAGG ACGACAGTGA AGGCGAGGAA AGCGAGGAGG AGGAGGAGGG CGAGGAGGAA GGCTCCGAGT CTGAGTCCCG CTCCGTCAAG GTGAAGATCA AGCTGGGCCG CAAGGAGAAG GCCCAGGACC GACTCAAGGG GGGCCGCCGG CGGCCAAGCC GGGGATCCCG GGCCAAGCCG GTTGTGAGTG ACGATGACAG TGAGGAGGAG CAGGAGGAGG ACCGCTCAGG AAGTGGCAGT GAGGAAGACG AATTCGACTA CAAGGACGAC GATGATAAGT GACTCGAGCA TGCATCTAGA GGGCCCTATT CTATAGTGTC ACCTAAATGC TAGAGCTCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG CATCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGGGGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTC
