pPLPR-OmpA-TSHR(human)
$99.00
In stock
SKU
PL49655
NM_000369.5;CHNG1; hTSHR-I; LGR3
100μg liquid plasmid(Instantly):$120
| name | pPLPR-OmpA-TSHR(human) | another name | apPLPR-OmpA-TSHR(human) |
| Insert Size (bp) | 2295bp | Plasmid host | Escherichia coli |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | PL,PR | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTCTTAAAAA TTAAGCCCTG AAGAAGGGCA GCATTCAAAG CAGAAGGCTT TGGGGTGTGT GATACGAAAC GAAGCATTGG TTAAAAATTA AGGAGGGAAT TCATGTTTCA CACAGGAAAC AGCTATGACC ATGATTACGG ATTCACTGGA ACTCTAGATA ACGAGGCGCA AAAAATGAAA AAGACAGCTA TCGCGATTGC AGTGGCACTG GCAGGTTTCG CTACCGTCGC TCAGGCTATG AGGCCGGCGG ACTTGCTGCA GCTGGTGCTG CTGCTCGACC TGCCCAGGGA CCTGGGCGGA ATGGGGTGTT CGTCTCCACC CTGCGAGTGC CATCAGGAGG AGGACTTCAG AGTCACCTGC AAGGATATTC AACGCATCCC CAGCTTACCG CCCAGTACGC AGACTCTGAA GCTTATTGAG ACTCACCTGA GAACTATTCC AAGTCATGCA TTTTCTAATC TGCCCAATAT TTCCAGAATC TACGTATCTA TAGATGTGAC TCTGCAGCAG CTGGAATCAC ACTCCTTCTA CAATTTGAGT AAAGTGACTC ACATAGAAAT TCGGAATACC AGGAACTTAA CTTACATAGA CCCTGATGCC CTCAAAGAGC TCCCCCTCCT AAAGTTCCTT GGCATTTTCA ACACTGGACT TAAAATGTTC CCTGACCTGA CCAAAGTTTA TTCCACTGAT ATATTCTTTA TACTTGAAAT TACAGACAAC CCTTACATGA CGTCAATCCC TGTGAATGCT TTTCAGGGAC TATGCAATGA AACCTTGACA CTGAAGCTGT ACAACAATGG CTTTACTTCA GTCCAAGGAT ATGCTTTCAA TGGGACAAAG CTGGATGCTG TTTACCTAAA CAAGAATAAA TACCTGACAG TTATTGACAA AGATGCATTT GGAGGAGTAT ACAGTGGACC AAGCTTGCTG GACGTGTCTC AAACCAGTGT CACTGCCCTT CCATCCAAAG GCCTGGAGCA CCTGAAGGAA CTGATAGCAA GAAACACCTG GACTCTTAAG AAACTTCCAC TTTCCTTGAG TTTCCTTCAC CTCACACGGG CTGACCTTTC TTACCCAAGC CACTGCTGTG CTTTTAAGAA TCAGAAGAAA ATCAGAGGAA TCCTTGAGTC CTTGATGTGT AATGAGAGCA GTATGCAGAG CTTGCGCCAG AGAAAATCTG TGAATGCCTT GAATAGCCCC CTCCACCAGG AATATGAAGA GAATCTGGGT GACAGCATTG TTGGGTACAA GGAAAAGTCC AAGTTCCAGG ATACTCATAA CAACGCTCAT TATTACGTCT TCTTTGAAGA ACAAGAGGAT GAGATCATTG GTTTTGGCCA GGAGCTCAAA AACCCCCAGG AAGAGACTCT ACAAGCTTTT GACAGCCATT ATGACTACAC CATATGTGGG GACAGTGAAG ACATGGTGTG TACCCCCAAG TCCGATGAGT TCAACCCGTG TGAAGACATA ATGGGCTACA AGTTCCTGAG AATTGTGGTG TGGTTCGTTA GTCTGCTGGC TCTCCTGGGC AATGTCTTTG TCCTGCTTAT TCTCCTCACC AGCCACTACA AACTGAACGT CCCCCGCTTT CTCATGTGCA ACCTGGCCTT TGCGGATTTC TGCATGGGGA TGTACCTGCT CCTCATCGCC TCTGTAGACC TCTACACTCA CTCTGAGTAC TACAACCATG CCATCGACTG GCAGACAGGC CCTGGGTGCA ACACGGCTGG TTTCTTCACT GTCTTTGCAA GCGAGTTATC GGTGTATACG CTGACGGTCA TCACCCTGGA GCGCTGGTAT GCCATCACCT TCGCCATGCG CCTGGACCGG AAGATCCGCC TCAGGCACGC ATGTGCCATC ATGGTTGGGG GCTGGGTTTG CTGCTTCCTT CTCGCCCTGC TTCCTTTGGT GGGAATAAGT AGCTATGCCA AAGTCAGTAT CTGCCTGCCC ATGGACACCG AGACCCCTCT TGCTCTGGCA TATATTGTTT TTGTTCTGAC GCTCAACATA GTTGCCTTCG TCATCGTCTG CTGCTGTTAT GTGAAGATCT ACATCACAGT CCGAAATCCG CAGTACAACC CAGGGGACAA AGATACCAAA ATTGCCAAGA GGATGGCTGT GTTGATCTTC ACCGACTTCA TATGCATGGC CCCAATCTCA TTCTATGCTC TGTCAGCAAT TCTGAACAAG CCTCTCATCA CTGTTAGCAA CTCCAAAATC TTGCTGGTAC TCTTCTATCC ACTTAACTCC TGTGCCAATC CATTCCTCTA TGCTATTTTC ACCAAGGCCT TCCAGAGGGA TGTGTTCATC CTACTCAGCA AGTTTGGCAT CTGTAAACGC CAGGCTCAGG CATACCGGGG GCAGAGGGTT CCTCCAAAGA ACAGCACTGA TATTCAGGTT CAAAAGGTTA CCCACGAGAT GAGGCAGGGT CTCCACAACA TGGAAGATGT CTATGAACTG ATTGAAAACT CCCATCTAAC CCCAAAGAAG CAAGGCCAAA TCTCAGAAGA GTATATGCAA ACGGTTTTGT AAGGATCCGT CGACCTGCAG CCAAGCTTGG CTGTTTTGGC GGATGAGAGA AGATTTTCAG CCTGATACAG ATTAAATCAG AACGCAGAAG CGGTCTGATA AAACAGAATT TGCCTGGCGG CAGTAGCGCG GTGGTCCCAC CTGACCCCAT GCCGAACTCA GAAGTGAAAC GCCGTAGCGC CGATGGTAGT GTGGGGTCTC CCCATGCGAG AGTAGGGAAC TGCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG CCGGGAGCGG ATTTGAACGT TGCGAAGCAA CGGCCCGGAG GGTGGCGGGC AGGACGCCCG CCATAAACTG CCAGGCATCA AATTAAGCAG AAGGCCATCC TGACGGATGG CCTTTTTGCG TTTCTACAAA CTCTTTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCTTATCTTT CCCTTTATTT TTGCTGCGGT AAGTCGCATA AAAACCATTC TTCATAATTC AATCCATTTA CTATGTTATG TTCTGAGGGG AGTGAAAATT CCCCTAATTC GATGAAGATT CTTGCTCAAT TGTTATCAGC TATGCGCCGA CCAGAACACC TTGCCGATCA GCCAAACGTC TCTTCAGGCC ACTGACTAGC GATAACTTTC CCCACAACGG AACAACTCTC ATTGCATGGG ATCATTGGGT ACTGTGGGTT TAGTGGTTGT AAAAACACCT GACCGCTATC CCTGATCAGT TTCTTGAAGG TAAACTCATC ACCCCCAAGT CTGGCTATGC AGAAATCACC TGGCTCAACA GCCTGCTCAG GGTCAACGAG AATTAACATT CCGTCAGGAA AGCTTGGCTT GGAGCCTGTT GGTGCGGTCA TGGAATTACC TTCAACCTCA AGCCAGAATG CAGAATCACT GGCTTTTTTG GTTGTGCTTA CCCATCTCTC CGCATCACCT TTGGTAAAGG TTCTAAGCTT AGGTGAGAAC ATCCCTGCCT GAACATGAGA AAAAACAGGG TACTCATACT CACTTCTAAG TGACGGCTGC ATACTAACCG CTTCATACAT CTCGTAGATT TCTCTGGCGA TTGAAGGGCT AAATTCTTCA ACGCTAACTT TGAGAATTTT TGTAAGCAAT GCGGCGTTAT AAGCATTTAA TGCATTGATG CCATTAAATA AAGCACCAAC GCCTGACTGC CCCATCCCCA TCTTGTCTGC GACAGATTCC TGGGATAAGC CAAGTTCATT TTTCTTTTTT TCATAAATTG CTTTAAGGCG ACGTGCGTCC TCAAGCTGCT CTTGTGTTAA TGGTTTCTTT TTTGTGCTCA TACGTTAAAT CTATCACCGC AAGGGATAAA TATCTAACAC CGTGCGTGTT GACTATTTTA CCTCTGGCGG TGATAATGGT TGCATGTACT AAGGAGGTTG TATGGAACAA CGCATAACCC TGAAAGATTA TGCAATGCGC TTTGGGCAAA CCAAGACAGC TAAAGATCTC TCACCTACCA AACAATGCCC CCCTGCAAAA AATAAATTCA TATAAAAAAC ATACAGATAA CCATCTGCGG TGATAAATTA TCTCTGGCGG TGTTGACATA AATACCACTG GCGGTGATAC TGAGCACATC AGCAGGACGC ACTGACCACC ATGAAGGTGA CG
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