pJ23119-EGFP-pJ23119-SUL1-Tet-tetG
$113.00
In stock
SKU
PL49628
100μg liquid plasmid(Instantly):$134
| name | pJ23119-EGFP-pJ23119-SUL1-Tet-tetG | another name | apJ23119-EGFP-pJ23119-SUL1-Tet-tetG |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | J23119 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTGACAGCT AGCTCAGTCC TAGGTATAAT ACTAGTTTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTTAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAGCT GATAGACTTC GAAATTTGAC AGCTAGCTCA GTCCTAGGTA TAATACTAGT TTTCACACAG GAAACAGTTG TTTAACTTTA AGAAGGAGTT ACGCCAAGCT TATGGTGACG GTGTTCGGCA TTCTGAATCT CACCGAGGAC TCCTTCTTCG ATGAGAGCCG GCGGCTAGAC CCCGCCGGCG CTGTCACCGC GGCGATCGAA ATGCTGCGAG TCGGATCAGA CGTCGTGGAT GTCGGACCGG CCGCCAGCCA TCCGGACGCG AGGCCTGTAT CGCCGGCCGA TGAGATCAGA CGTATTGCGC CGCTCTTAGA CGCCCTGTCC GATCAGATGC ACCGTGTTTC AATCGACAGC TTCCAACCGG AAACCCAGCG CTATGCGCTC AAGCGCGGCG TGGGCTACCT GAACGATATC CAAGGATTTC CTGACCCTGC GCTCTATCCC GATATTGCTG AGGCGGACTG CAGGCTGGTG GTTATGCACT CAGCGCAGCG GGATGGCATC GCCACCCGCA CCGGTCACCT TCGACCCGAA GACGCGCTCG ACGAGATTGT GCGGTTCTTC GAGGCGCGGG TTTCCGCCTT GCGACGGAGC GGGGTCGCTG CCGACCGGCT CATCCTCGAT CCGGGGATGG GATTTTTCTT GAGCCCCGCA CCGGAAACAT CGCTGCACGT GCTGTCGAAC CTTCAAAAGC TGAAGTCGGC GTTGGGGCTT CCGCTATTGG TCTCGGTGTC GCGGAAATCC TTCTTGGGCG CCACCGTTGG CCTTCCTGTA AAGGATCTGG GTCCAGCGAG CCTTGCGGCG GAACTTCACG CGATCGGCAA TGGCGCTGAC TACGTCCGCA CCCACGCGCC TGGAGATCTG CGAAGCGCAA TCACCTTCTC GGAAACCCTC GCGAAATTTC GCAGTCGCGA CGCCAGAGAC CGAGGGTTAG ATCATGCCTG AAAGCGACAG GCCATGTTTG ACAGCTTATC ATCGATAAGC TTTAATGCGG TAGTTTATCA CAGTTAAATT GCTAACGCAA TGGGTCTCGG CCTCATCATG CCCGTCCTTC CGACGCTTCT GCGTGAGCTT GTGCCAGCAG AGCAGGTCGC TGGACACTAT GGTGCCTTGC TGTCGCTCTA TGCATTGATG CAGGTCGTCT TCGCGCCCAT GCTTGGACAG CTTTCGGATT CTTACGGTCG GCGTCCGGTA CTTCTGGCTT CTCTTGCAGG AGCCGCAGTC GATTACACGA TTATGGCATC AGCGCCGGTC TTATGGGTGC TCTATATCGG CCGACTCGTG TCCGGCGTCA CGGGCGCAAC CGGAGCTGTA GCAGCCTCAA CCATTGCCGA TTCGACGGGG GAAGGTTCTC GCGCACGCTG GTTCGGCTAC ATGGGGGCCT GTTATGGGGC GGGCATGATT GCCGGGCCAG CACTTGGTGG CATGCTCGGT GGTATCTCTG CTCATGCCCC GTTTATCGCC GCCGCCCTTC TCAACGGGTT CGCGTTCCTG CTTGCCTGCA TTTTCCTCAA GGAGACTCAT CACAGCCATG GCGGGACCGG AAAGCCGGTT CGCATCAAAC CATTCGTTCT GTTACGGCTG GATGATGCAT TGCGCGGGCT AGGTGCGCTT TTCGCAGTTT TCTTCATTAT TCAACTGATC GGCCAAGTGC CTGCAGCCCT ATGGGTCATA TATGGCGAGG ACCGTTTTCA GTGGAACACC GCGACCGTTG GTTTGTCGCT CGCGGCGTTT GGGGCAACAC ATGCGATCTT CCAAGCGTTT GTTACCGGCC CGCTTTCAAG CCGGCTTGGA GAGCGGCGCA CGCTGCTGTT TGGCATGGCT GCGGATGCGA CTGGCTTCGT TCTTCTGGCT TTTGCCACGC AGGGATGGAT GGTGTTCCCG ATTCTGTTGC TGCTTGCCGC CGGGGGTGTT GGCATGCCGG CCTTGCAGGC AATGCTCTCA AACAATGTCA GCAGTAACAA GCAAGGGGCT TTGCAAGGAA CGCTAACGAG CCTCACCAAT CTAAGCTCTA TCGCAGGACC GCTTGGCTTC ACAGCACTCT ATTCTGCCAC CGCCGGGGCA TGGAACGGTT GGGTTTGGAT TGTCGGCGCG ATCCTCTATT TAATATGTCT GCCAATACTA CGCAGACCAT TCGCAACTTC ATTGTGAGAA TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
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