pUC-NCL(mouse)
$84.00
In stock
SKU
PL49569
NM_010880.3;B530004O11Rik; C23; D0Nds28; D1Nds28; Nucl
100μg liquid plasmid(Instantly):$105
| name | pUC-NCL(mouse) | another name | apUC-NCL(mouse) |
| Insert Size (bp) | 2124bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGT ACCTCGCGAA TGCATCTAGA TATGGTGAAG CTCGCAAAGG CTGGCAAAAC CCACGGTGAG GCCAAGAAAA TGGCTCCTCC TCCAAAGGAG GTGGAAGAGG ATAGTGAAGA TGAAGAAATG TCAGAAGATG AAGATGACAG CAGTGGAGAA GAGGAGGTTG TCATCCCTCA GAAAAAAGGC AAAAAGGCTA CCACAACCCC AGCAAAGAAG GTGGTTGTTT CACAAACAAA AAAGGCTGCA GTTCCCACAC CAGCTAAGAA AGCAGCTGTG ACCCCAGGCA AAAAGGCAGT AGCCACACCA GCTAAGAAAA ACATTACACC AGCCAAAGTC ATTCCAACAC CGGGTAAGAA GGGAGCTGCA CAAGCAAAAG CGTTGGTACC AACTCCTGGT AAAAAGGGAG CTGCCACTCC AGCTAAGGGG GCTAAGAACG GTAAGAATGC CAAGAAGGAA GACAGTGATG AGGATGAAGA TGAAGAGGAT GAAGATGATA GCGATGAGGA TGAAGATGAT GAGGAAGAGG ATGAGTTTGA GCCACCAATA GTAAAAGGAG TGAAGCCAGC AAAAGCAGCT CCTGCTGCTC CTGCCTCAGA GGATGAGGAA GATGATGAGG ATGAAGATGA TGAGGAAGAT GATGATGAAG AGGAGGAAGA TGACTCTGAG GAAGAAGTTA TGGAGATCAC AACAGCCAAA GGAAAGAAAA CTCCTGCAAA AGTTGTTCCT ATGAAAGCCA AGAGTGTGGC TGAGGAGGAG GATGATGAGG AAGAGGATGA AGATGACGAG GATGAGGATG ATGAGGAAGA GGATGACGAA GATGATGATG AGGAAGAAGA GGAGGAAGAA CCTGTTAAAG CAGCACCTGG AAAACGGAAG AAGGAGATGA CCAAGCAGAA AGAAGCCCCT GAAGCCAAGA AACAGAAAGT AGAAGGCTCA GAACCAACTA CACCTTTCAA TCTGTTCATT GGAAACCTTA ATCCAAACAA GTCTGTTAAT GAATTAAAAT TTGCCATCAG TGAACTTTTT GCTAAAAATG ATCTTGCTGT TGTGGATGTC AGAACTGGTA CAAATAGGAA ATTTGGTTAT GTGGACTTTG AGTCTGCTGA AGACCTAGAA AAGGCCTTGG AGCTCACTGG TTTAAAAGTG TTTGGCAATG AAATTAAACT AGAAAAACCA AAAGGAAGAG ATAGTAAGAA AGTTCGAGCT GCAAGAACAC TTCTAGCCAA AAACCTCTCT TTCAACATCA CTGAGGATGA ATTAAAGGAA GTGTTTGAAG ACGCCATGGA GATCAGATTA GTCAGCCAGG ATGGGAAAAG TAAAGGGATT GCTTATATTG AATTTAAGTC TGAAGCTGAT GCAGAGAAAA ATTTGGAAGA AAAGCAGGGG GCAGAAATTG ATGGACGATC TGTTTCACTC TACTATACTG GAGAGAAAGG TCAAAGGCAA GAGAGAACTG GAAAGACCAG CACTTGGAGT GGTGAATCAA AGACTTTGGT TTTAAGTAAC CTTTCCTACA GTGCAACAAA AGAAACTCTT GAGGAAGTAT TTGAGAAAGC AACTTTTATC AAAGTGCCCC AGAACCCACA TGGCAAACCT AAAGGGTATG CATTTATAGA ATTTGCTTCA TTTGAAGATG CTAAAGAAGC TTTAAATTCC TGTAATAAAA TGGAAATTGA GGGCAGAACA ATCAGGCTGG AGTTGCAAGG ATCCAATTCG AGAAGTCAAC CATCCAAAAC TCTGTTTGTC AAAGGTCTGT CTGAGGATAC CACTGAAGAG ACCTTAAAAG AATCATTTGA GGGCTCTGTT CGTGCAAGAA TAGTCACTGA TCGGGAAACT GGTTCTTCCA AAGGGTTTGG TTTTGTAGAC TTTAATAGTG AGGAAGATGC CAAAGCTGCC AAGGAGGCCA TGGAAGATGG AGAAATTGAC GGAAACAAAG TTACCTTGGA CTGGGCCAAA CCTAAGGGTG AAGGTGGCTT TGGTGGTCGA GGTGGAGGCA GAGGAGGTTT CGGAGGCAGA GGTGGAGGCA GAGGTGGAAG AGGTGGATTT GGAGGAAGAG GCCGGGGAGG CTTTGGAGGT AGAGGAGGCT TCCGAGGCGG CAGAGGAGGA GGGGGAGACT TCAAGCCACA AGGAAAGAAG ACGAAGTTTG AATAGATCGG ATCCCGGGCC CGTCGACTGC AGAGGCCTGC ATGCAAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTC
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