pLV2-TRE3GS-TURBID-G74-Myc-UB(human)-TetOne-Hyg
$99.00
In stock
SKU
PL49410
100μg liquid plasmid(Instantly):$120
| name | pLV2-TRE3GS-TURBID-G74-Myc-UB(human)-TetOne-Hyg | another name | apLV2-TRE3GS-TURBID-G74-Myc-UB(human)-TetOne-Hyg |
| Insert Size (bp) | Plasmid host | Mammary cell,Lentiviral | |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | TRE3GS | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTACTAAGT TTGTAGTACA TATTTAACAA ATACAATTTC TTTAAAAACT ACTAAGTTTG TAGTACATAT TTAACAAATA CAATTTCTTT AAAATGAAAA TAATTCAGAG GAATCACAGG TTTAGAGTAA ATGAAACCAC TAGGTAATTG GCAGTGGTAA TAGGGTATGG GGTGGGAAGT TTGGGATGAT TTTGGTTAGC TTGAGTTATC CAGTTGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT GATCTCCCGA TCCGTCGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCT AGCTAGAGGT CGACGGTAAA ACGCTAGCAA TGTCAAGGCC TCTCACTCTC TGATATTCAT TTCTTTGCAA GTTATAAATA CTGAATAATA AGATGACATG AACTACTACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGGCA AAAAGCAGCT GCTTATATGC AGGATCTGAG GGCTCGCCAC TCCCCAGTCC CGCCCAGGCC ACGCCTCCCT GGAAAGTCCC CAGCGGAAAG TCCCTTGTAG CAAGCTCGAT ATCAGCAGTT CTTGAAGTAC TCCGGATGCA GCTCTCGGGC CACGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AACACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCTGGTG TTCTCTCCTT TATTGGCCTC TTCTACCTTA TCTGGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGACCCCT GGCCCTGGTG TGTAGTTCTG CTAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCT CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACTGCAGAA TTAATTCCAG GCGGGGAGGC GGCCCAAAGG GAGATCCGAC TCGTCTGAGG GCGAAGGCGA AGACGCGGAA GAGGCCGCAG AGCCGGCAGC AGGCCGCGGG AAGGAAGGTC CGCTGGATTG AGGGCCGAAG GGACGTAGCA GAAGGACGTC CCGCGCAGAA TCCAGGTGGC AACACAGGCG AGCAGCCATG GAAAGGACGT CAGCTTCCCC GACAACACCA CGGAATTGTC AGTGCCCAAC AGCCGAGCCC CTGTCCAGCA GCGGGCAAGG CAGGCGGCGA TGAGTTCCGC CGTGGCAATA GGGAGGGGGA AAGCGAAAGT CCCGGAAAGG AGCTGACAGG TGGTGGCAAT GCCCCAACCA GTGGGGGTTG CGTCAGCAAA CACAGTGCAC ACCACGCCAC GTTGCCTGAC AACGGGCCAC AACTCCTCAT AAAGAGACAG CAACCAGGAT TTATACAAGG AGGAGAAAAT GAAAGCCATA CGGGAAGCAA TAGCATGATA CAAAGGCATT AAAGCAGCGT ATCCACATAG CGTAAAAGGA GCAACATAGT TAAGAATACC AGTCAATCTT TCACAAATTT TGTAATCCAG AGGTTGATTG TTCCAGACGC GTCTATTCCT TTGCCCTCGG ACGAGTGCTG GGGCGTCGGT TTCCACTATC GGCGAGTACT TCTACACAGC CATCGGTCCA GACGGCCGCG CTTCTGCGGG CGATTTGTGT ACGCCCGACA GTCCCGGCTC CGGATCGGAC GATTGCGTCG CATCGACCCT GCGCCCAAGC TGCATCATCG AAATTGCCGT CAACCAAGCT CTGATAGAGT TGGTCAAGAC CAATGCGGAG CATATACGCC CGGAGCCGCG GCGATCCTGC AAGCTCCGGA TGCCTCCGCT CGAAGTAGCG CGTCTGCTGC TCCATACAAG CCAACCACGG CCTCCAGAAG AAGATGTTGG CGACCTCGTA TTGGGAATCC CCGAACATCG CCTCGCTCCA GTCAATGACC GCTGTTATGC GGCCATTGTC CGTCAGGACA TTGTTGGAGC CGAAATCCGC GTGCACGAGG TGCCGGACTT CGGGGCAGTC CTCGGCCCAA AGCATCAGCT CATCGAGAGC CTGCGCGACG GACGCACTGA CGGTGTCGTC CATCACAGTT TGCCAGTGAT ACACATGGGG ATCAGCAATC GCGCATATGA AATCACGCCA TGTAGTGTAT TGACCGATTC CTTGCGGTCC GAATGGGCCG AACCCGCTCG TCTGGCTAAG ATCGGCCGCA GCGATCGCAT CCATGGCCTC CGCGACCGGC TGCAGAACAG CGGGCAGTTC GGTTTCAGGC AGGTCTTGCA ACGTGACACC CTGTGCACGG CGGGAGATGC AATAGGTCAG GCTCTCGCTG AATTCCCCAA TGTCAAGCAC TTCCGGAATC GGGAGCGCGG CCGATGCAAA GTGCCGATAA ACATAACGAT CTTTGTAGAA ACCATCGGCG CAGCTATTTA CCCGCAGGAC ATATCCACGC CCTCCTACAT CGAAGCTGAA AGCACGAGAT TCTTCGCCCT CCGAGAGCTG CATCAGGTCG GAGACGCTGT CGAACTTTTC GATCAGAAAC TTCTCGACAG ACGTCGCGGT GAGTTCAGGC TTTTTCATCA CGTGCTGATC AGATCCGAAA ATGGATATAC AAGCTCCCGG GAGCTTTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC GCGCGTTTAC CCGGGGAGCA TGTCAAGGTC AAAATCGTCA AGAGCGTCAG CAGGCAGCAT ATCAAGGTCA AAGTCGTCAA GGGCATCGGC TGGGAGCATG TCTAAGTCAA AATCGTCAAG GGCGTCGGTC GGCCCGCCGC TTTCGCACTT TAGCTGTTTC TCCAGGCCAC ATATGATTAG TTCCAGGCCG AAAAGGAAGG CAGGTTCGGC TCCCTGCCGG TCGAACAGCT CAATTGCTTG TTTCAGAAGT GGGGGCATAG AATCGGTGGT AGGTGTCTCT CTTTCCTCTT TTGCTACTTG ATGCTCCTGT TCCTCCAATA CGCAGCCCAG TGTAAAGTGG CCCACGGCGG ACAGAGCGTA CAGTGCGTTC TCCAGGGAGA AGCCTTGCTG ACACAGGAAC GCGAGCTGAT TTTCCAGGGT TTCGTACTGT TTCTCTGTTG GGCGGGTGCC GAGATGCACT TTAGCCCCGT CGCGATGTGA GAGGAGAGCA CAGCGGTATG ACTTGGCGTT GTTCCGCAGA AAGTCTTGCC ATGACTCGCC TTCCAGGGGG CAGGAGTGGG TATGATGCCT GTCCAGCATC TCGATTGGCA GGGCATCGAG CAGGGCCCGC TTGTTCTTCA CGTGCCAGTA CAGGGTAGGC TGCTCAACTC CCAGCTTTTG AGCGAGTTTC CTTGTCGTCA GGCCTTCGAT ACCGACTCCA TTGAGTAATT CCAGAGCAGA GTTTATGACT TTGCTCTTGT CCAGTCTAGA CATGGTAATT CGATGATCCC CCTGGGGAGA GAGGTCGGTG ATTCGGTCAA CGAGGGAGCC GACTGCCGAC GTGCGCTCCG GAGGCTTGCA GAATGCGGAA CACCGCGCGG GCAGGAACAG GGCCCACACT ACCGCCCCAC ACCCCGCCTC CCGCACCGCC CCTTCCCGGC CGCTGCTCTC GGCGCGCCCT GCTGAGCAGC CGCTATTGGC CACAGCCCAT CGCGGTCGGC GCGCTGCCAT TGCTCCCTGG CGCTGTCCGT CTGCGAGGGT ACTAGTGAGA CGTGCGGCTT CCGTTTGTCA CGTCCGGCAC GCCGCGAACC GCAAGGAACC TTCCCGACTT AGGGGCGGAG CAGGAAGCGT CGCCGGGGGG CCCACAAGGG TAGCGGCGAA GATCCGGGTG ACGCTGCGAA CGGACGTGAA GAATGTGCGA GACCCAGGGT CGGCGCCGCT GCGTTTCCCG GAACCACGCC CAGAGCAGCC GCGTCCCTGC GCAAACCCAG GGCTGCCTTG GAAAAGGCGC AACCCCAACC CCGTGGAATT ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC AGTGATAGAG AACGTATAAG CTTTGCTTAT GTAAACCAGG GCGCCTATAA AAGAGTGCTG ATTTTTTGAG TAAACTTCAA TTCCACAACA CTTTTGTCTT ATACCAACTT TCCGTACCAC TTCCTACCCT CGTAAAGAAT TCCCGTATAC ACCGGTGCCA CCATGGGACA GCTGGACGGC GGGAGCGTGG CAGTCCTGCC TGTGGTCGAC TCCACCAATC AGTACCTGCT GGATCGAATC GGCGAGCTGA AGAGTGGGGA TGCTTGCATT GCAGAATATC AGCAGGCAGG GAGAGGAAGC AGAGGGAGGA AATGGTTCTC TCCTTTTGGA GCTAACCTGT ACCTGAGTAT GTTTTGGCGC CTGAAGCGGG GACCAGCAGC AATCGGCCTG GGCCCGGTCA TCGGAATTGT CATGGCAGAA GCGCTGCGAA AGCTGGGAGC AGACAAGGTG CGAGTCAAAT GGCCCAATGA CCTGTATCTG CAGGATAGAA AGCTGGCAGG CATCCTGGTG GAGCTGGCCG GAATAACAGG CGATGCTGCA CAGATCGTCA TTGGCGCCGG GATTAACGTG GCTATGAGGC GCGTGGAGGA AAGCGTGGTC AATCAGGGCT GGATCACACT GCAGGAAGCA GGGATTAACC TGGACAGGAA TACTCTGGCC GCTACGCTGA TCCGAGAGCT GCGGGCAGCC CTGGAACTGT TCGAGCAGGA AGGCCTGGCT CCATATCTGC CACGGTGGGA GAAGCTGGAT AACTTCATCA ATAGACCCGT GAAGCTGATC ATTGGGGACA AAGAGATTTT CGGGATTAGC CGGGGGATTG ATAAACAGGG AGCCCTGCTG CTGGAACAGG ACGGAGTTAT CAAACCCTGG ATGGGCGGAG AAATCAGTCT GCGGTCTGCC GAAAAGGGCT CGGGCGGGAG TACCGGTGAA CAAAAACTCA TCTCAGAAGA GGATCTGGGT AGTGGGAGCG GTATGCAGAT CTTCGTGAAA ACCCTTACCG GCAAGACCAT CACCCTTGAG GTGGAGCCCA GTGACACCAT CGAAAATGTG AAGGCCAAGA TCCAGGATAA GGAAGGCATT CCCCCCGACC AGCAGAGGCT CATCTTTGCA GGCAAGCAGC TGGAAGATGG CCGTACTCTT TCTGACTACA ACATCCAGAA GGAGTCGACC CTGCACCTGG TCCTGCGTCC AAGAGGTGGT TAAGGATCCA GACCACCTCC CCTGCGAGCT AAGCTGGACA GCCAATGACG GGTAAGAGAG TGACATTTTT CACTAACCTA AGACAGGAGG GCCGTCAGAG CTACTGCCTA ATCCAAAGAC GGGTAAAAGT GATAAAAATG TATCACTCCA ACCTAAGACA GGCGCAGCTT CCGAGGGATT TGAGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCAAAAC TAGAATGCAG TGAAAAAAAT GCCTTATTTG TGAAATTTGT GATGCTATTG CCTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA GTCGATAAAC TGGATCTCTG CTGTCCCTGT AATAAACCCG AAAATTTTGA ATTTTTGTAA TTTGTTTTTG TAGTTCTTTA GTTTGTATGT CTGTTGCTAT TATGTCTACT ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAGGC GATACCGTCG AGATCCGTTC ACTAATCGAA TGGATCTGTC TCTGTCTCTC TCTCCACCTT CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG GTCTGAAACG ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA AAGTACAGCA AAAACTATTC TTAAACCTAC CAAGCCTCCT ACTATCATTA TGAATAATTT TATATACCAC AGCCAATTTG TTATGTTAAA CCAATTCCAC AAACTTGCCC ATTTATCTAA TTCCAATAAT TCTTGTTCAT TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAAGGAGTG TATTAAGCTT GTGTAATTGT TAATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGATTCCA AATCTGTTCC AGAGATTTAT TACTCCAACT AGCATTCCAA GGCACAGCAG TGGTGCAAAT GAGTTTTCCA GAGCAACCCC AAATCCCCAG GAGCTGTTGA TCCTTTAGGT ATCTTTCCAC AGCCAGGATT CTTGCCTGGA GCTGCTTGAT GCCCCAGACT GTGAGTTGCA ACAGATGCTG TTGCGCCTCA ATAGCCCTCA GCAAATTGTT CTGCTGCTGC ACTATACCAG ACAATAATTG TCTGGCCTGT ACCGTCAGCG TCATTGACGC TGCGCCCATA GTGCTTCCTG CTGCTCCCAA GAACCCAAGG AACAAAGCTC CTATTCCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTTG GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGGCC GCTTGCTGTG CGGTGGTCTT ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT TTATCTCTAT CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA GTTCTTCTGA TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT CTGATGTTTC TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA CTATATGTTT TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC GCGATCTAAT TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC CTCCGCTAGT CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC GCGCTTCAGC AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA AGTCCCTGTT CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGGC AAAAAGCAGC TGCTTATATG CAGGATCTGA GGGCTCGCCA CTCCCCAGTC CCGCCCAGGC CACGCCTCCC TGGAAAGTCC CCAGCGGAAA GTCCCTTGTA GCAAGCTCGA TATCAGCAGT TCTTGAAGTA CTCCGGATGC AGCTCTCGGG CCACGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAACACTTCT CTCTCCGGGT CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCTGGT GTTCTCTCCT TTATTGGCCT CTTCTACCTT ATCTGGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGACCCC TGGCCCTGGT GTGTAGTTCT GCTAATCAGG GAAGTAGCCT TGTGTGTGGT AGATCCACAG ATCAAGGATA TCTTGTCTTC TTTGGGAGTG AATTAGCCCT TCCA
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