pJ23119-MCS-sgRNA-SacB-Str
$84.00
In stock
SKU
PL49320
100μg liquid plasmid(Instantly):$105
| name | pJ23119-MCS-sgRNA-SacB-Str | another name | apJ23119-MCS-sgRNA-SacB-Str |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Str | |
| Selective marker | Fluorescent labeling | ||
| Promoter | J23119 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGGGCCTAAA GAACTAAAAA ATCTATCTGT TTCTTTTCAT TCTCTGTATT TTTTATAGTT TCTGTTGCAT GGGCATAAAG TTGCAAGCTT GATATCTTGA CAGCTAGCTC AGTCCTAGGT ATAATACTAG TCGAGACCAT TGGTCTCAGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG CACCGAGTCG GTGCTTTTTT TGATATCGAA TTCCTGCAGC CCGGGGGATC CACTAGTTCT AGAGCGGCCG CCACCGCGGT GGAGCTCATC CCAATGGCGC GCCGAGCTTG GCTCGAGCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTATTTGC CGACTACCTT GGTGATCTCG CCTTTCACGT AGTGGACAAA TTCTTCCAAC TGATCTGCGC GCGAGGCCAA GCGATCTTCT TCTTGTCCAA GATAAGCCTG TCTAGCTTCA AGTATGACGG GCTGATACTG GGCCGGCAGG CGCTCCATTG CCCAGTCGGC AGCGACATCC TTCGGCGCGA TTTTGCCGGT TACTGCGCTG TACCAAATGC GGGACAACGT AAGCACTACA TTTCGCTCAT CGCCAGCCCA GTCGGGCGGC GAGTTCCATA GCGTTAAGGT TTCATTTAGC GCCTCAAATA GATCCTGTTC AGGAACCGGA TCAAAGAGTT CCTCCGCCGC TGGACCTACC AAGGCAACGC TATGTTCTCT TGCTTTTGTC AGCAAGATAG CCAGATCAAT GTCGATCGTG GCTGGCTCGA AGATACCTGC AAGAATGTCA TTGCGCTGCC ATTCTCCAAA TTGCAGTTCG CGCTTAGCTG GATAACGCCA CGGAATGATG TCGTCGTGCA CAACAATGGT GACTTCTACA GCGCGGAGAA TCTCGCTCTC TCCAGGGGAA GCCGAAGTTT CCAAAAGGTC GTTGATCAAA GCTCGCCGCG TTGTTTCATC AAGCCTTACG GTCACCGTAA CCAGCAAATC AATATCACTG TGTGGCTTCA GGCCGCCATC CACTGCGGAG CCGTACAAAT GTACGGCCAG CAACGTCGGT TCGAGATGGC GCTCGATGAC GCCAACTACC TCTGATAGTT GAGTCGATAC TTCGGCGATC ACCGCTTCCC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AATTGACGCG TATTGGGATG GTACCGGGCC CCCCCTCGAG GTCGACGGTA TCGATACGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TAATCGGCAT TTTCTTTTGC GTTTTTATTT GTTAACTGTT AATTGTCCTT GTTCAAGGAT GCTGTCTTTG ACAACAGATG TTTTCTTGCC TTTGATGTTC AGCAGGAAGC TAGGCGCAAA CGTTGATTGT TTGTCTGCGT AGAATCCTCT GTTTGTCATA TAGCTTGTAA TCACGACATT GTTTCCTTTC GCTTGAGGTA CAGCGAAGTG TGAGTAAGTA AAGGTTACAT CGTTAGGATC AAGATCCATT TTTAACACAA GGCCAGTTTT GTTCAGCGGC TTGTATGGGC CAGTTAAAGA ATTAGAAACA TAACCAAGCA TGTAAATATC GTTAGACGTA ATGCCGTCAA TCGTCATTTT TGATCCGCGG GAGTCAGTGA ACAGATACCA TTTGCCGTTC ATTTTAAAGA CGTTCGCGCG TTCAATTTCA TCTGTTACTG TGTTAGATGC AATCAGCGGT TTCATCACTT TTTTCAGTGT GTAATCATCG TTTAGCTCAA TCATACCGAG AGCGCCGTTT GCTAACTCAG CCGTGCGTTT TTTATCGCTT TGCAGAAGTT TTTGACTTTC TTGACGGAAG AATGATGTGC TTTTGCCATA GTATGCTTTG TTAAATAAAG ATTCTTCGCC TTGGTAGCCA TCTTCAGTTC CAGTGTTTGC TTCAAATACT AAGTATTTGT GGCCTTTATC TTCTACGTAG TGAGGATCTC TCAGCGTATG GTTGTCGCCT GAGCTGTAGT TGCCTTCATC GATGAACTGC TGTACATTTT GATACGTTTT TCCGTCACCG TCAAAGATTG ATTTATAATC CTCTACACCG TTGATGTTCA AAGAGCTGTC TGATGCTGAT ACGTTAACTT GTGCAGTTGT CAGTGTTTGT TTGCCGTAAT GTTTACCGGA GAAATCAGTG TAGAATAAAC GGATTTTTCC GTCAGATGTA AATGTGGCTG AACCTGACCA TTCTTGTGTT TGGTCTTTTA GGATAGAATC ATTTGCATCG AATTTGTCGC TGTCTTTAAA GACGCGGCCA GCGTTTTTCC AGCTGTCAAT AGAAGTTTCG CCGACTTTTT GATAGAACAT GTAAATCGAT GTGTCATCCG CATTTTTAGG ATCTCCGGCT AATGCAAAGA CGATGTGGTA GCCGTGATAG TTTGCGACAG TGCCGTCAGC GTTTTGTAAT GGCCAGCTGT CCCAAACGTC CAGGCCTTTT GCAGAAGAGA TATTTTTAAT TGTGGACGAA TCGAACTCAG GAACTTGATA TTTTTCATTT TTTTGCTGTT CAGGGATTTG CAGCATATCA TGGCGTGTAA TATGGGAAAT GCCGTATGTT TCCTTATATG GCTTTTGGTT CGTTTCTTTC GCAAACGCTT GAGTTGCGCC TCCTGCCAGC AGTGCGGTAG TAAAGGTTAA TACTGTTGCT TGTTTTGCAA ACTTTTTGAT GTTCATCGTT CATGTCTCCT TTTTTATGTA CTGTGTTAGC GGTCTGCTTC TTCCAGCCCT CCTGTTTGAA GATGGCAAGT TAGTTACGCA CAATAAAAAA AGACCTAAAA TATGTAAGGG GTGACGCCAA AGTATACACT TTGCCCTTTA CACATTTTAG GTCTTGCCTG CTTTATCAGT AACAAACCCG CGCGATTTAC TTTTCGACCT CATTCTATTA GACTCTCGTT TGGATTGCAA CTGGTCTATT TTCCTCTTTT GTTTGATAGA AAATCATAAA AGGATTTGCA GACTA
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