pMAS-GUS-HA-35S-Hyg
$84.00
In stock
SKU
PL49291
100μg liquid plasmid(Instantly):$105
| name | pMAS-GUS-HA-35S-Hyg | another name | apMAS-GUS-HA-35S-Hyg |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Hyg | Fluorescent labeling | Gus |
| Promoter | Replicon | pVS1 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGCTGCTCTA GCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC ACGGTATCGA TAAGCTCGCG GATCCCTGAA AGCGACGTTG GATGTTAACA TCTACAAATT GCCTTTTCTT ATCGACCATG TACGTAAGCG CTTACGTTTT TGGTGGACCC TTGAGGAAAC TGGTAGCTGT TGTGGGCCTG TGGTCTCAAG ATGGATCATT AATTTCCACC TTCACCTACG ATGGGGGGCA TCGCACCGGT GAGTAATATT GTACGGCTAA GAGCGAATTT GGCCTGTAGG ATCCCTGAAA GCGACGTTGG ATGTTAACAT CTACAAATTG CCTTTTCTTA TCGACCATGT ACGTAAGCGC TTACGTTTTT GGTGGACCCT TGAGGAAACT GGTAGCTGTT GTGGGCCTGT GGTCTCAAGA TGGATCATTA ATTTCCACCT TCACCTACGA TGGGGGGCAT CGCACCGGTG AGTAATATTG TACGGCTAAG AGCGAATTTG GCCTGTAGGA TCCCTGAAAG CGACGTTGGA TGTTAACATC TACAAATTGC CTTTTCTTAT CGACCATGTA CGTAAGCGCT TACGTTTTTG GTGGACCCTT GAGGAAACTG GTAGCTGTTG TGGGCCTGTG GTCTCAAGAT GGATCATTAA TTTCCACCTT CACCTACGAT GGGGGGCATC GCACCGGTGA GTAATATTGT ACGGCTAAGA GCGAATTTGG CCTGTAGGAT CCGCGAGCTG GTCAATCCCA TTGCTTTTGA AGCAGCTCAA CATTGATCTC TTTCTCGATC GAGGGAGATT TTTCAAATCA GTGCGCAAGA CGTGACGTAA GTATCCGAGT CAGTTTTTAT TTTTCTACTA ATTTGGTCGT TTATTTCGGC GTGTAGGACA TGGCAACCGG GCCTGAATTT CGCGGGTATT CTGTTTCTAT TCCAACTTTT TCTTGATCCG CAGCCATTAA CGACTTTTGA ATAGATACGC TGACACGCCA AGCCTCGCTA GTCAAAAGTG TACCAAACAA CGCTTTACAG CAAGAACGGA ATGCGCGTGA CGCTCGCGGT GACGCCATTT CGCCTTTTCA GAAATGGATA AATAGCCTTG CTTCCTATTA TATCTTCCCA AATTACCAAT ACATTACACT AGCATCTGAA TTTCATAACC AATCTCGATA CACCAAATCG ACTCTAGAAA GCTTCTGCAG GGGCCCGGGG TCGACATTTA AATACTAGTG GATCCGGTAC CATGGTAGAT CTGACTAGTT TACGTCCTGT AGAAACCCCA ACCCGTGAAA TCAAAAAACT CGACGGCCTG TGGGCATTCA GTCTGGATCG CGAAAACTGT GGAATTGATC AGCGTTGGTG GGAAAGCGCG TTACAAGAAA GCCGGGCAAT TGCTGTGCCA GGCAGTTTTA ACGATCAGTT CGCCGATGCA GATATTCGTA ATTATGCGGG CAACGTCTGG TATCAGCGCG AAGTCTTTAT ACCGAAAGGT TGGGCAGGCC AGCGTATCGT GCTGCGTTTC GATGCGGTCA CTCATTACGG CAAAGTGTGG GTCAATAATC AGGAAGTGAT GGAGCATCAG GGCGGCTATA CGCCATTTGA AGCCGATGTC ACGCCGTATG TTATTGCCGG GAAAAGTGTA CGTATCACCG TTTGTGTGAA CAACGAACTG AACTGGCAGA CTATCCCGCC GGGAATGGTG ATTACCGACG AAAACGGCAA GAAAAAGCAG TCTTACTTCC ATGATTTCTT TAACTATGCC GGAATCCATC GCAGCGTAAT GCTCTACACC ACGCCGAACA CCTGGGTGGA CGATATCACC GTGGTGACGC ATGTCGCGCA AGACTGTAAC CACGCGTCTG TTGACTGGCA GGTGGTGGCC AATGGTGATG TCAGCGTTGA ACTGCGTGAT GCGGATCAAC AGGTGGTTGC AACTGGACAA GGCACTAGCG GGACTTTGCA AGTGGTGAAT CCGCACCTCT GGCAACCGGG TGAAGGTTAT CTCTATGAAC TGTGCGTCAC AGCCAAAAGC CAGACAGAGT GTGATATCTA CCCGCTTCGC GTCGGCATCC GGTCAGTGGC AGTGAAGGGC CAACAGTTCC TGATTAACCA CAAACCGTTC TACTTTACTG GCTTTGGTCG TCATGAAGAT GCGGACTTAC GTGGCAAAGG ATTCGATAAC GTGCTGATGG TGCACGACCA CGCATTAATG GACTGGATTG GGGCCAACTC CTACCGTACC TCGCATTACC CTTACGCTGA AGAGATGCTC GACTGGGCAG ATGAACATGG CATCGTGGTG ATTGATGAAA CTGCTGCTGT CGGCTTTCAG CTGTCTTTAG GCATTGGTTT CGAAGCGGGC AACAAGCCGA AAGAACTGTA CAGCGAAGAG GCAGTCAACG GGGAAACTCA GCAAGCGCAC TTACAGGCGA TTAAAGAGCT GATAGCGCGT GACAAAAACC ACCCAAGCGT GGTGATGTGG AGTATTGCCA ACGAACCGGA TACCCGTCCG CAAGGTGCAC GGGAATATTT CGCGCCACTG GCGGAAGCAA CGCGTAAACT CGACCCGACG CGTCCGATCA CCTGCGTCAA TGTAATGTTC TGCGACGCTC ACACCGATAC CATCAGCGAT CTCTTTGATG TGCTGTGCCT GAACCGTTAT TACGGATGGT ATGTCCAAAG CGGCGATTTG GAAACGGCAG AGAAGGTACT GGAAAAAGAA CTTCTGGCCT GGCAGGAGAA ACTGCATCAG CCGATTATCA TCACCGAATA CGGCGTGGAT ACGTTAGCCG GGCTGCACTC AATGTACACC GACATGTGGA GTGAAGAGTA TCAGTGTGCA TGGCTGGATA TGTATCACCG CGTCTTTGAT CGCGTCAGCG CCGTCGTCGG TGAACAGGTA TGGAATTTCG CCGATTTTGC GACCTCGCAA GGCATATTGC GCGTTGGCGG TAACAAGAAA GGGATCTTCA CTCGCGACCG CAAACCGAAG TCGGCGGCTT TTCTGCTGCA AAAACGCTGG ACTGGCATGA ACTTCGGTGA AAAACCGCAG CAGGGAGGCA AACAAGCTAG CTACCCATAC GATGTTCCAG ATTACGCTTG AGCTCGAATT TCCCCGATCG TTCAAACATT TGGCAATAAA GTTTCTTAAG ATTGAATCCT GTTGCCGGTC TTGCGATGAT TATCATATAA TTTCTGTTGA ATTACGTTAA GCATGTAATA ATTAACATGT AATGCATGAC GTTATTTATG AGATGGGTTT TTATGATTAG AGTCCCGCAA TTATACATTT AATACGCGAT AGAAAACAAA ATATAGCGCG CAAACTAGGA TAAATTATCG CGCGCGGTGT CATCTATGTT ACTAGATCGG GAATTCGTAA TCATGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG CTAGAGCAGC TTGCCAACAT GGTGGAGCAC GACACTCTCG TCTACTCCAA GAATATCAAA GATACAGTCT CAGAAGACCA AAGGGCTATT GAGACTTTTC AACAAAGGGT AATATCGGGA AACCTCCTCG GATTCCATTG CCCAGCTATC TGTCACTTCA TCAAAAGGAC AGTAGAAAAG GAAGGTGGCA CCTACAAATG CCATCATTGC GATAAAGGAA AGGCTATCGT TCAAGATGCC TCTGCCGACA GTGGTCCCAA AGATGGACCC CCACCCACGA GGAGCATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC AAAGCAAGTG GATTGATGTG ATATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA TCCTTCGCAA GACCCTTCCT CTATATAAGG AAGTTCATTT CATTTGGAGA GGACACGCTG AAATCACCAG TCTCTCTCTA CAAATCTATC TCTCTCGAGC TTTCGCAGAT CCGGGGGGCA ATGAGATATG AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC GAAGAATCTC GTGCTTTCAG CTTCGATGTA GGAGGGCGTG GATATGTCCT GCGGGTAAAT AGCTGCGCCG ATGGTTTCTA CAAAGATCGT TATGTTTATC GGCACTTTGC ATCGGCCGCG CTCCCGATTC CGGAAGTGCT TGACATTGGG GAGTTTAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTT CACAGGGTGT CACGTTGCAA GACCTGCCTG AAACCGAACT GCCCGCTGTT CTACAACCGG TCGCGGAGGC TATGGATGCG ATCGCTGCGG CCGATCTTAG CCAGACGAGC GGGTTCGGCC CATTCGGACC GCAAGGAATC GGTCAATACA CTACATGGCG TGATTTCATA TGCGCGATTG CTGATCCCCA TGTGTATCAC TGGCAAACTG TGATGGACGA CACCGTCAGT GCGTCCGTCG CGCAGGCTCT CGATGAGCTG ATGCTTTGGG CCGAGGACTG CCCCGAAGTC CGGCACCTCG TGCACGCGGA TTTCGGCTCC AACAATGTCC TGACGGACAA TGGCCGCATA ACAGCGGTCA TTGACTGGAG CGAGGCGATG TTCGGGGATT CCCAATACGA GGTCGCCAAC ATCTTCTTCT GGAGGCCGTG GTTGGCTTGT ATGGAGCAGC AGACGCGCTA CTTCGAGCGG AGGCATCCGG AGCTTGCAGG ATCGCCACGA CTCCGGGCGT ATATGCTCCG CATTGGTCTT GACCAACTCT ATCAGAGCTT GGTTGACGGC AATTTCGATG ATGCAGCTTG GGCGCAGGGT CGATGCGACG CAATCGTCCG ATCCGGAGCC GGGACTGTCG GGCGTACACA AATCGCCCGC AGAAGCGCGG CCGTCTGGAC CGATGGCTGT GTAGAAGTAC TCGCCGATAG TGGAAACCGA CGCCCCAGCA CTCGTCCGAG GGCAAAGAAA TAGAGTAGAT GCCGACCGGG ATCTGTCGAT CGACAAGCTC GAGTTTCTCC ATAATAATGT GTGAGTAGTT CCCAGATAAG GGAATTAGGG TTCCTATAGG GTTTCGCTCA TGTGTTGAGC ATATAAGAAA CCCTTAGTAT GTATTTGTAT TTGTAAAATA CTTCTATCAA TAAAATTTCT AATTCCTAAA ACCAAAATCC AGTACTAAAA TCCAGATCCC CCGAATTAAT TCGGCGTTAA TTCAGTACAT TAAAAACGTC CGCAATGTGT TATTAAGTTG TCTAAGCGTC AATTTGTTTA CACCACAATA TATCCTGCCA CCAGCCAGCC AACAGCTCCC CGACCGGCAG CTCGGCACAA AATCACCACT CGATACAGGC AGCCCATCAG TCCGGGACGG CGTCAGCGGG AGAGCCGTTG TAAGGCGGCA GACTTTGCTC ATGTTACCGA TGCTATTCGG AAGAACGGCA ACTAAGCTGC CGGGTTTGAA ACACGGATGA TCTCGCGGAG GGTAGCATGT TGATTGTAAC GATGACAGAG CGTTGCTGCC TGTGATCACC GCGGTTTCAA AATCGGCTCC GTCGATACTA TGTTATACGC CAACTTTGAA AACAACTTTG AAAAAGCTGT TTTCTGGTAT TTAAGGTTTT AGAATGCAAG GAACAGTGAA TTGGAGTTCG TCTTGTTATA ATTAGCTTCT TGGGGTATCT TTAAATACTG TAGAAAAGAG GAAGGAAATA ATAAATGGCT AAAATGAGAA TATCACCGGA ATTGAAAAAA CTGATCGAAA AATACCGCTG CGTAAAAGAT ACGGAAGGAA TGTCTCCTGC TAAGGTATAT AAGCTGGTGG GAGAAAATGA AAACCTATAT TTAAAAATGA CGGACAGCCG GTATAAAGGG ACCACCTATG ATGTGGAACG GGAAAAGGAC ATGATGCTAT GGCTGGAAGG AAAGCTGCCT GTTCCAAAGG TCCTGCACTT TGAACGGCAT GATGGCTGGA GCAATCTGCT CATGAGTGAG GCCGATGGCG TCCTTTGCTC GGAAGAGTAT GAAGATGAAC AAAGCCCTGA AAAGATTATC GAGCTGTATG CGGAGTGCAT CAGGCTCTTT CACTCCATCG ACATATCGGA TTGTCCCTAT ACGAATAGCT TAGACAGCCG CTTAGCCGAA TTGGATTACT TACTGAATAA CGATCTGGCC GATGTGGATT GCGAAAACTG GGAAGAAGAC ACTCCATTTA AAGATCCGCG CGAGCTGTAT GATTTTTTAA AGACGGAAAA GCCCGAAGAG GAACTTGTCT TTTCCCACGG CGACCTGGGA GACAGCAACA TCTTTGTGAA AGATGGCAAA GTAAGTGGCT TTATTGATCT TGGGAGAAGC GGCAGGGCGG ACAAGTGGTA TGACATTGCC TTCTGCGTCC GGTCGATCAG GGAGGATATC GGGGAAGAAC AGTATGTCGA GCTATTTTTT GACTTACTGG GGATCAAGCC TGATTGGGAG AAAATAAAAT ATTATATTTT ACTGGATGAA TTGTTTTAGT ACCTAGAATG CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GGGTGCCTTG ATGTGGGCGC CGGCGGTCGA GTGGCGACGG CGCGGCTTGT CCGCGCCCTG GTAGATTGCC TGGCCGTAGG CCAGCCATTT TTGAGCGGCC AGCGGCCGCG ATAGGCCGAC GCGAAGCGGC GGGGCGTAGG GAGCGCAGCG ACCGAAGGGT AGGCGCTTTT TGCAGCTCTT CGGCTGTGCG CTGGCCAGAC AGTTATGCAC AGGCCAGGCG GGTTTTAAGA GTTTTAATAA GTTTTAAAGA GTTTTAGGCG GAAAAATCGC CTTTTTTCTC TTTTATATCA GTCACTTACA TGTGTGACCG GTTCCCAATG TACGGCTTTG GGTTCCCAAT GTACGGGTTC CGGTTCCCAA TGTACGGCTT TGGGTTCCCA ATGTACGTGC TATCCACAGG AAAGAGACCT TTTCGACCTT TTTCCCCTGC TAGGGCAATT TGCCCTAGCA TCTGCTCCGT ACATTAGGAA CCGGCGGATG CTTCGCCCTC GATCAGGTTG CGGTAGCGCA TGACTAGGAT CGGGCCAGCC TGCCCCGCCT CCTCCTTCAA ATCGTACTCC GGCAGGTCAT TTGACCCGAT CAGCTTGCGC ACGGTGAAAC AGAACTTCTT GAACTCTCCG GCGCTGCCAC TGCGTTCGTA GATCGTCTTG AACAACCATC TGGCTTCTGC CTTGCCTGCG GCGCGGCGTG CCAGGCGGTA GAGAAAACGG CCGATGCCGG GATCGATCAA AAAGTAATCG GGGTGAACCG TCAGCACGTC CGGGTTCTTG CCTTCTGTGA TCTCGCGGTA CATCCAATCA GCTAGCTCGA TCTCGATGTA CTCCGGCCGC CCGGTTTCGC TCTTTACGAT CTTGTAGCGG CTAATCAAGG CTTCACCCTC GGATACCGTC ACCAGGCGGC CGTTCTTGGC CTTCTTCGTA CGCTGCATGG CAACGTGCGT GGTGTTTAAC CGAATGCAGG TTTCTACCAG GTCGTCTTTC TGCTTTCCGC CATCGGCTCG CCGGCAGAAC TTGAGTACGT CCGCAACGTG TGGACGGAAC ACGCGGCCGG GCTTGTCTCC CTTCCCTTCC CGGTATCGGT TCATGGATTC GGTTAGATGG GAAACCGCCA TCAGTACCAG GTCGTAATCC CACACACTGG CCATGCCGGC CGGCCCTGCG GAAACCTCTA CGTGCCCGTC TGGAAGCTCG TAGCGGATCA CCTCGCCAGC TCGTCGGTCA CGCTTCGACA GACGGAAAAC GGCCACGTCC ATGATGCTGC GACTATCGCG GGTGCCCACG TCATAGAGCA TCGGAACGAA AAAATCTGGT TGCTCGTCGC CCTTGGGCGG CTTCATAATC GACGGCGCAC CGGCTGCCGG CGGTTGCCGG GATTCTTTGC GGATTCGATC AGCGGCCGCT TGCCACGATT CACCGGGGCG TGCTTCTGCC TCGATGCGTT GCCGCTGGGC GGCCTGCGCG GCCTTCAACT TCTCCACCAG GTCATCACCC AGCGCCGCGC CGATTTGTAC CGGGCCGGAT GGTTTGCGAC CGCTCACGCC GATTCCTCGG GCTTGGGGGT TCCAGTGCCA TTGCAGGGCC GGCAGGCAAC CCAGCCGCTT ACGCCTGGCC AACCGCCCGT TCCTCCACAC ATGGGGCATT CCACGGCGTC GGTGCCTGGT TGTTCTTGAT TTTCCATGCC GCCTCCTTTA GCCGCTAAAA TTCATCTACT CATTTATTCA TTTGCTCATT TACTCTGGTA GCTGCGCGAT GTATTCAGAT AGCAGCTCGG TAATGGTCTT GCCTTGGCGT ACCGCGTACA TCTTCAGCTT GGTGTGATCC TCCGCCGGCA ACTGAAAGTT GACCCGCTTC ATGGCTGGCG TGTCTGCCAG GCTGGCCAAC GTTGCAGCCT TGCTGCTGCG TGCGCTCGGA CGGCCGGCAC TTAGCGTGTT TGTGCTTTTG CTCATTTTCT CTTTACCTCA TTAACTCAAA TGAGTTTTGA TTTAATTTCA GCGGCCAGCG CCTGGACCTC GCGGGCAGCG TCGCCCTCGG GTTCTGATTC AAGAACGGTT GTGCCGGCGG CGGCAGTGCC TGGGTAGCTC ACGCGCTGCG TGATACGGGA CTCAAGAATG GGCAGCTCGT ACCCGGCCAG CGCCTCGGCA ACCTCACCGC CGATGCGCGT GCCTTTGATC GCCCGCGACA CGACAAAGGC CGCTTGTAGC CTTCCATCCG TGACCTCAAT GCGCTGCTTA ACCAGCTCCA CCAGGTCGGC GGTGGCCCAT ATGTCGTAAG GGCTTGGCTG CACCGGAATC AGCACGAAGT CGGCTGCCTT GATCGCGGAC ACAGCCAAGT CCGCCGCCTG GGGCGCTCCG TCGATCACTA CGAAGTCGCG CCGGCCGATG GCCTTCACGT CGCGGTCAAT CGTCGGGCGG TCGATGCCGA CAACGGTTAG CGGTTGATCT TCCCGCACGG CCGCCCAATC GCGGGCACTG CCCTGGGGAT CGGAATCGAC TAACAGAACA TCGGCCCCGG CGAGTTGCAG GGCGCGGGCT AGATGGGTTG CGATGGTCGT CTTGCCTGAC CCGCCTTTCT GGTTAAGTAC AGCGATAACC TTCATGCGTT CCCCTTGCGT ATTTGTTTAT TTACTCATCG CATCATATAC GCAGCGACCG CATGACGCAA GCTGTTTTAC TCAAATACAC ATCACCTTTT TAGACGGCGG CGCTCGGTTT CTTCAGCGGC CAAGCTGGCC GGCCAGGCCG CCAGCTTGGC ATCAGACAAA CCGGCCAGGA TTTCATGCAG CCGCACGGTT GAGACGTGCG CGGGCGGCTC GAACACGTAC CCGGCCGCGA TCATCTCCGC CTCGATCTCT TCGGTAATGA AAAACGGTTC GTCCTGGCCG TCCTGGTGCG GTTTCATGCT TGTTCCTCTT GGCGTTCATT CTCGGCGGCC GCCAGGGCGT CGGCCTCGGT CAATGCGTCC TCACGGAAGG CACCGCGCCG CCTGGCCTCG GTGGGCGTCA CTTCCTCGCT GCGCTCAAGT GCGCGGTACA GGGTCGAGCG ATGCACGCCA AGCAGTGCAG CCGCCTCTTT CACGGTGCGG CCTTCCTGGT CGATCAGCTC GCGGGCGTGC GCGATCTGTG CCGGGGTGAG GGTAGGGCGG GGGCCAAACT TCACGCCTCG GGCCTTGGCG GCCTCGCGCC CGCTCCGGGT GCGGTCGATG ATTAGGGAAC GCTCGAACTC GGCAATGCCG GCGAACACGG TCAACACCAT GCGGCCGGCC GGCGTGGTGG TGTCGGCCCA CGGCTCTGCC AGGCTACGCA GGCCCGCGCC GGCCTCCTGG ATGCGCTCGG CAATGTCCAG TAGGTCGCGG GTGCTGCGGG CCAGGCGGTC TAGCCTGGTC ACTGTCACAA CGTCGCCAGG GCGTAGGTGG TCAAGCATCC TGGCCAGCTC CGGGCGGTCG CGCCTGGTGC CGGTGATCTT CTCGGAAAAC AGCTTGGTGC AGCCGGCCGC GTGCAGTTCG GCCCGTTGGT TGGTCAAGTC CTGGTCGTCG GTGCTGACGC GGGCATAGCC CAGCAGGCCA GCGGCGGCGC TCTTGTTCAT GGCGTAATGT CTCCGGTTCT AGTCGCAAGT ATTCTACTTT ATGCGACTAA AACACGCGAC AAGAAAACGC CAGGAAAAGG GCAGGGCGGC AGCCTGTCGC GTAACTTAGG ACTTGTGCGA CATGTCGTTT TCAGAAGACG GCTGCACTGA ACGTCAGAAG CCGACTGCAC TATAGCAGCG GAGGGGTTGG ATCAAAGTAC TTTGATCCCG AGGGGAACCC TGTGGTTGGC ATGCACATAC AAATGGACGA ACGGATAAAC CTTTTCACGC CCTTTTAAAT ATCCGATTAT TCTAATAAAC GCTCTTTTCT CTTAGGTTTA CCCGCCAATA TATCCTGTCA AACACTGATA GTTTAAACTG AAGGCGGGAA ACGACAATCT GATCCAAGCT CAAGCTGCTC TAGCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGG
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